BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30033 (558 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 253 1e-68 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 127 1e-30 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 113 2e-26 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 109 2e-25 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 91 1e-19 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 74 2e-14 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 69 6e-13 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 67 1e-12 SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 43 4e-05 SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccha... 27 1.9 SPBC16A3.08c |||nuclear telomere cap complex subunit |Schizosacc... 25 5.7 SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces... 25 7.5 SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase |Schizosaccharo... 25 10.0 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 253 bits (619), Expect = 1e-68 Identities = 116/135 (85%), Positives = 124/135 (91%) Frame = -2 Query: 557 ELPDGQVITIGNERFRCP*ALFQPSFMGMESCGIHETVYNSIMKCGVDIRKDLYANTVMS 378 ELPDGQVITIGNERFR P ALFQPS +G+E+ GIHE YNSIMKC VDIRKDLY N VMS Sbjct: 241 ELPDGQVITIGNERFRAPEALFQPSALGLENAGIHEATYNSIMKCDVDIRKDLYGNVVMS 300 Query: 377 GGTTMYPGIADRMQKEITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKE 198 GGTTMYPGIADRMQKEI ALAPS++K+KI+APPERKYSVWIGGSILASLSTFQQMWISK+ Sbjct: 301 GGTTMYPGIADRMQKEIQALAPSSMKVKIVAPPERKYSVWIGGSILASLSTFQQMWISKQ 360 Query: 197 EYDESGPGIVXRKCF 153 EYDESGPGIV RKCF Sbjct: 361 EYDESGPGIVYRKCF 375 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 127 bits (307), Expect = 1e-30 Identities = 58/135 (42%), Positives = 89/135 (65%) Frame = -2 Query: 557 ELPDGQVITIGNERFRCP*ALFQPSFMGMESCGIHETVYNSIMKCGVDIRKDLYANTVMS 378 +LPD Q IT+G E F P LF P MG E+ G+H ++ SI+ +D+R LY+N V+S Sbjct: 246 QLPDNQTITLGTECFSAPEVLFNPEMMGSEASGLHIQLFKSILLSDIDLRSTLYSNIVLS 305 Query: 377 GGTTMYPGIADRMQKEITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKE 198 GG+T+ G +R E+ A++ ++KI A PER ++ W+GGSILASLSTF+++ I+ E Sbjct: 306 GGSTLLRGFGERFISELRAISGKKNQVKIYASPERMHNAWLGGSILASLSTFRRLLITSE 365 Query: 197 EYDESGPGIVXRKCF 153 EY ++ ++ R+ F Sbjct: 366 EY-KNDQNVIFRRRF 379 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 113 bits (271), Expect = 2e-26 Identities = 58/137 (42%), Positives = 88/137 (64%), Gaps = 12/137 (8%) Frame = -2 Query: 554 LPDGQVITIGNERFRCP*ALFQPSFMGMESCGIHETVYNSIMKCGVDIRKDLYANTVMSG 375 LPDG+VI +G+ER+ CP LFQP +G E G+ E ++++I VDIRK LY V+SG Sbjct: 242 LPDGRVIKVGSERYECPECLFQPHLVGSEQPGLSEFIFDTIQAADVDIRKYLYRAIVLSG 301 Query: 374 GTTMYPGIADRMQKEITAL--------APSTI---KIKIIAPPERKYSVWIGGSILAS-L 231 G++MY G+ R++KEI L P+ + K+KI P R+++V+IGG++LA + Sbjct: 302 GSSMYAGLPSRLEKEIKQLWFERVLHGDPARLPNFKVKIEDAPRRRHAVFIGGAVLADIM 361 Query: 230 STFQQMWISKEEYDESG 180 + MW+SK E++E G Sbjct: 362 AQNDHMWVSKAEWEEYG 378 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 109 bits (263), Expect = 2e-25 Identities = 59/137 (43%), Positives = 77/137 (56%), Gaps = 11/137 (8%) Frame = -2 Query: 557 ELPDGQVITIGNERFRCP*ALFQPSFMGMESC---------GIHETVYNSIMKCGVDIRK 405 E PDG G ERFR LF PSF S G+HE VY SI+ C ++R Sbjct: 282 EFPDGVTHKFGQERFRISEILFNPSFSASRSAETTPPQGSVGLHELVYQSILACDSELRS 341 Query: 404 DLYANTVMSGGTTMYPGIADRMQKEITALAP-STIKIKII-APPERKYSVWIGGSILASL 231 L N V++GGT++ PG+++R+Q E+ LA S I + +VW GGSILASL Sbjct: 342 PLLNNIVVTGGTSLIPGLSERLQAEVQRLATGSRINVHTAETASATSNAVWFGGSILASL 401 Query: 230 STFQQMWISKEEYDESG 180 FQ +W+SK+EYDE G Sbjct: 402 DNFQHLWVSKQEYDEVG 418 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 90.6 bits (215), Expect = 1e-19 Identities = 45/108 (41%), Positives = 69/108 (63%), Gaps = 6/108 (5%) Frame = -2 Query: 461 GIHETVYNSIMKCGVDIRKDLYANTVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAP 282 GI + N I +C VDIR L N ++ GGT++ G + R+Q E++ L P + ++KI A Sbjct: 326 GIPQLFQNCISECDVDIRASLLNNVIVCGGTSLMQGFSLRLQNELSKLYPGS-RLKIHAS 384 Query: 281 P---ERKYSVWIGGSILASLSTFQQMWISKEEYDESGP---GIVXRKC 156 ER Y+ W+GGSIL+SL TF Q+WIS++EY+E G ++ ++C Sbjct: 385 GHVVERSYASWLGGSILSSLGTFHQLWISRQEYEEHGSDRLALIEKRC 432 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 73.7 bits (173), Expect = 2e-14 Identities = 42/128 (32%), Positives = 63/128 (49%) Frame = -2 Query: 542 QVITIGNERFRCP*ALFQPSFMGMESCGIHETVYNSIMKCGVDIRKDLYANTVMSGGTTM 363 QV+ + NERF P LF PS + + GI E V S+ +I+ L N V GG Sbjct: 272 QVLNLSNERFMIPELLFSPSDIEIREAGIPEAVMQSVTHFPENIQAMLLENVVTIGGNCK 331 Query: 362 YPGIADRMQKEITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDES 183 +PG R+ E+ +LAP+ ++K+ P + W S + L + I++ EY E Sbjct: 332 FPGFHKRLSSELRSLAPANWEVKVFEPSDPICFPWKKASHM-PLEHWNANKITRSEYSEH 390 Query: 182 GPGIVXRK 159 G I+ RK Sbjct: 391 GANIMTRK 398 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 68.5 bits (160), Expect = 6e-13 Identities = 40/140 (28%), Positives = 66/140 (47%), Gaps = 15/140 (10%) Frame = -2 Query: 536 ITIGNERFRCP*ALFQPSFMGMESCG-IHETVYNSIMKCGVDIRKDLYANTVMSGGTTMY 360 I +G ERF P F P + + E V N + +D+RK LY N V+SGG+T++ Sbjct: 280 IDVGFERFLAPEIFFNPEIASSDFLTPLPELVDNVVQSSPIDVRKGLYKNIVLSGGSTLF 339 Query: 359 PGIADRMQKEITALAPSTI--------------KIKIIAPPERKYSVWIGGSILASLSTF 222 +R+Q+++ + I + +I+ ++ +VW GGS+LA F Sbjct: 340 KNFGNRLQRDLKRIVDERIHRSEMLSGAKSGGVDVNVISHKRQRNAVWFGGSLLAQTPEF 399 Query: 221 QQMWISKEEYDESGPGIVXR 162 +K +Y+E G I R Sbjct: 400 GSYCHTKADYEEYGASIARR 419 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 67.3 bits (157), Expect = 1e-12 Identities = 31/115 (26%), Positives = 61/115 (53%) Frame = -2 Query: 521 ERFRCP*ALFQPSFMGMESCGIHETVYNSIMKCGVDIRKDLYANTVMSGGTTMYPGIADR 342 E+ R P +F PS +G++ GI E + + + + ++ ++ L +N +++GG PG+ R Sbjct: 594 EQIRVPEVIFSPSIVGIDQAGILEIMRSILQRHSLEEQQKLVSNVLITGGLGSLPGMETR 653 Query: 341 MQKEITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGP 177 +++E+T++ P I + W G S + F+ +++EEY E GP Sbjct: 654 IKRELTSIMPVGSSINVFRASNPLLDAWKGASEWSVTEKFKAAKVTREEYLEKGP 708 >SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 620 Score = 42.7 bits (96), Expect = 4e-05 Identities = 20/86 (23%), Positives = 42/86 (48%), Gaps = 3/86 (3%) Frame = -2 Query: 407 KDLYANTVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAPP---ERKYSVWIGGSILA 237 K+LY++ ++ GG +PG A +++ I + + I +I PP + ++ W G I Sbjct: 527 KNLYSSILIVGGAGQFPGFAHLLEERIHSKRANIPTISVIPPPRSMDAQFVAWKGACIYN 586 Query: 236 SLSTFQQMWISKEEYDESGPGIVXRK 159 + ++WI ++ G ++ K Sbjct: 587 RIRIVSELWIKNSDWKMLGSRVLQYK 612 >SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 617 Score = 27.1 bits (57), Expect = 1.9 Identities = 14/37 (37%), Positives = 20/37 (54%) Frame = -2 Query: 335 KEITALAPSTIKIKIIAPPERKYSVWIGGSILASLST 225 KEI++ A S I + + P E+ W ILA L+T Sbjct: 212 KEISSQARSNIALPTLRPQEKNLIDWRDRKILAPLTT 248 >SPBC16A3.08c |||nuclear telomere cap complex subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 284 Score = 25.4 bits (53), Expect = 5.7 Identities = 15/43 (34%), Positives = 20/43 (46%) Frame = -1 Query: 456 PRDRVQLHHEVRRRHP*GPVRQHRHVRWYHHVPRYRRQDAEGD 328 PR+ V R+R P P + R V PR RRQ +G+ Sbjct: 41 PRELVAQSTTSRKRDPNQPTPRERTVNKKADQPRRRRQAPQGN 83 >SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces pombe|chr 3|||Manual Length = 879 Score = 25.0 bits (52), Expect = 7.5 Identities = 11/24 (45%), Positives = 15/24 (62%) Frame = -1 Query: 309 DHQDQDHRSPREEVLRMDRWIHPG 238 D D+ R+ E VLRM R++ PG Sbjct: 499 DIDDKKDRALSEHVLRMHRYLPPG 522 >SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase |Schizosaccharomyces pombe|chr 2|||Manual Length = 689 Score = 24.6 bits (51), Expect = 10.0 Identities = 12/32 (37%), Positives = 17/32 (53%) Frame = +1 Query: 268 YFLSGGAMILILMVEGARAVISFCILSAIPGY 363 Y +SGGA + +A +S CI +PGY Sbjct: 412 YCISGGAAL----AASTQAFLSSCICPVLPGY 439 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,031,439 Number of Sequences: 5004 Number of extensions: 37955 Number of successful extensions: 115 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 102 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 109 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 233995432 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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