Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV30031
(994 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces... 282 5e-77
SPAC4A8.05c |myp2|myo3|myosin II heavy chain |Schizosaccharomyce... 262 5e-71
SPCC1919.10c |myo52||myosin type V|Schizosaccharomyces pombe|chr... 209 5e-55
SPBC2D10.14c |myo51||myosin type V|Schizosaccharomyces pombe|chr... 201 1e-52
SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2... 173 2e-44
SPBC16H5.10c |prp43||ATP-dependent RNA helicase Prp43|Schizosacc... 30 0.44
SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual 28 2.3
SPCC1919.03c |||AMP-activated protein kinase beta subunit |Schiz... 27 3.1
SPAC3G6.01 |hrp3||ATP-dependent DNA helicase Hrp3|Schizosaccharo... 27 5.4
SPAC23C4.02 |crn1||actin binding protein, coronin Crn1|Schizosac... 27 5.4
SPBPB21E7.07 |aes1|SPAPB21E7.07, SPAPB21E7.07|enhancer of RNA-me... 26 7.2
SPBC887.14c |pfh1|pif1|pif1 helicase homolog Pfh1|Schizosaccharo... 26 7.2
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 26 9.5
SPAC4G8.05 |ppk14||serine/threonine protein kinase Ppk14 |Schizo... 26 9.5
>SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1526
Score = 282 bits (692), Expect = 5e-77
Identities = 140/283 (49%), Positives = 192/283 (67%), Gaps = 3/283 (1%)
Frame = +2
Query: 149 KPYDGKKACWVPDEKEGFVQGEIKATKGD---LVTVNLPGGETKDFKKDQVAQVNPPKYE 319
K D K+ W+ D + F + IK D +V N E K +D++ VNP K++
Sbjct: 19 KDIDDKRWVWISDPETAFTKAWIKEDLPDKKYVVRYNNSRDE-KIVGEDEIDPVNPAKFD 77
Query: 320 KCEDMSNLTYLNDASVLYNLKQRYYHKLIYTYSGLFCVAINPYKRFPVYTTRCAKLYRGK 499
+ DM+ LTYLN+ +V YNL+QRY IYTYSGLF VA+NPY P+YT +LY+ K
Sbjct: 78 RVNDMAELTYLNEPAVTYNLEQRYLSDQIYTYSGLFLVAVNPYCGLPIYTKDIIQLYKDK 137
Query: 500 RRSEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASQKKD 679
+ PH+FAI+D AY N+L N ENQS+L+TGESGAGKTENTK++I Y A + +S
Sbjct: 138 TQERKLPHVFAIADLAYNNLLENKENQSILVTGESGAGKTENTKRIIQYLAAIASSTTVG 197
Query: 680 PSQEKKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPSGKLAGADIETYL 859
SQ +E+Q+++TNPVLE+FGNA+TVRN+NSSRFGKFI++ F SG+++ A IE YL
Sbjct: 198 SSQ-----VEEQIIKTNPVLESFGNARTVRNNNSSRFGKFIKVEFSLSGEISNAAIEWYL 252
Query: 860 LEKARVISQQALERSYHIFYQMMSGSVNGLKAICLLSNDVMDY 988
LEK+RV+ Q ER+YH+FYQ++SG+ LK LL+++ DY
Sbjct: 253 LEKSRVVHQNEFERNYHVFYQLLSGADTALKNKLLLTDNCNDY 295
>SPAC4A8.05c |myp2|myo3|myosin II heavy chain |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2104
Score = 262 bits (642), Expect = 5e-71
Identities = 133/280 (47%), Positives = 177/280 (63%), Gaps = 1/280 (0%)
Frame = +2
Query: 155 YDGKKACWVPDEKEGFVQGEIKATKGDLVTVNLP-GGETKDFKKDQVAQVNPPKYEKCED 331
+D + W+PD KE FV+ I G+ V L G + +VNPPK++ +D
Sbjct: 34 FDERTWIWIPDSKESFVKAWIVEDLGEKYRVKLERDGSERIVDGFDAEKVNPPKFDMVDD 93
Query: 332 MSNLTYLNDASVLYNLKQRYYHKLIYTYSGLFCVAINPYKRFPVYTTRCAKLYRGKRRSE 511
M+ LT LN+ SV+ NL QRY LIYTYSGLF VA+NPY P+Y + Y+ K+ E
Sbjct: 94 MAALTCLNEPSVVNNLTQRYEKDLIYTYSGLFLVAVNPYCHLPIYGDDVVRKYQSKQFKE 153
Query: 512 VPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASQKKDPSQE 691
PHIF +D AY ++L NQS+L+TGESGAGKTE TKKVI Y +V + D Q
Sbjct: 154 TKPHIFGTADAAYRSLLERRINQSILVTGESGAGKTETTKKVIQYLTSVTDASTSDSQQ- 212
Query: 692 KKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPSGKLAGADIETYLLEKA 871
LE ++++TNPVLEAFGNA+TVRN+NSSRFGKFIRI F +G + GA+++ YLLEK+
Sbjct: 213 ----LEKKILETNPVLEAFGNAQTVRNNNSSRFGKFIRIEFSNNGSIVGANLDWYLLEKS 268
Query: 872 RVISQQALERSYHIFYQMMSGSVNGLKAICLLSNDVMDYN 991
RVI + ER+YH+FYQ++ G+ L L V Y+
Sbjct: 269 RVIHPSSNERNYHVFYQLLRGADGSLLESLFLDRYVDHYS 308
>SPCC1919.10c |myo52||myosin type V|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1516
Score = 209 bits (510), Expect = 5e-55
Identities = 109/273 (39%), Positives = 171/273 (62%), Gaps = 18/273 (6%)
Frame = +2
Query: 173 CWVPDEKEGFVQGEIKATKGDLVTVNLPGGETKDFKKDQVAQVNPPKYE----------- 319
CW+PDE+ ++ G IK + + L + + + + + YE
Sbjct: 12 CWIPDEQSQWIPGSIKDCRVEGEKAFLTVQDENENETVITVKPDDLNYEGRNGLPFLRSI 71
Query: 320 --KCEDMSNLTYLNDASVLYNLKQRYYHKLIYTYSGLFCVAINPYKRFP-VYTTRCAKLY 490
+D+++L+YLN+ SVL L RY IYTYSG+ +A+NP++R P +YT + Y
Sbjct: 72 NSDADDLTDLSYLNEPSVLDALSTRYNQLQIYTYSGIVLIAVNPFQRLPNLYTHEIVRAY 131
Query: 491 RGKRRSEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGAS- 667
K R E+ PH++AI++ +Y M H+NQ+++I+GESGAGKT + + ++ YFA+V A
Sbjct: 132 SEKSRDELDPHLYAIAEDSYKCMNQEHKNQTIIISGESGAGKTVSARYIMRYFASVQALI 191
Query: 668 QKKDPSQEKKGSL---EDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPSGKLAG 838
Q D + + L E++++ TNP++EAFGN+KT RNDNSSRFGK+I+I F + + G
Sbjct: 192 QSTDSNFHEAPQLTAVENEILATNPIMEAFGNSKTSRNDNSSRFGKYIQILFDGNATIIG 251
Query: 839 ADIETYLLEKARVISQQALERSYHIFYQMMSGS 937
A I+TYLLE++R++ Q ER+YHIFYQ+++GS
Sbjct: 252 AKIQTYLLERSRLVFQPNQERNYHIFYQILAGS 284
>SPBC2D10.14c |myo51||myosin type V|Schizosaccharomyces pombe|chr
2|||Manual
Length = 1471
Score = 201 bits (490), Expect = 1e-52
Identities = 108/260 (41%), Positives = 158/260 (60%), Gaps = 6/260 (2%)
Frame = +2
Query: 173 CWVPDEKEGFVQG---EIKATKGDLV--TVNLPGGETKDFKKDQVAQVNPPKYEKCEDMS 337
CWV + + EIK G V TV G + Q+ N + E D++
Sbjct: 12 CWVSNNNGHWDAARLIEIKDNGGGKVVATVAKSSGVLETVNYQQLQNRNIGQSESPSDLT 71
Query: 338 NLTYLNDASVLYNLKQRYYHKLIYTYSGLFCVAINPYKRFP-VYTTRCAKLYRGKRRSEV 514
NL YLN+ SVL+ L RY +K IYTYSG+ V+INPY+ P Y K + +
Sbjct: 72 NLPYLNEPSVLHALHNRYNNKQIYTYSGIVLVSINPYQNLPEFYNDNLIKHFHKDPEAAK 131
Query: 515 PPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASQKKDPSQEK 694
PH+++I+ Y + T+ +NQ+++++GESGAGKT K ++ Y +V Q D +
Sbjct: 132 VPHLYSIASSCYHALTTDSKNQTIIVSGESGAGKTVAAKYIMRYLTSV---QGVDHNGVV 188
Query: 695 KGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPSGKLAGADIETYLLEKAR 874
K S+E+QV+ TNP++EAFGNAKT+RNDNSSRFGK++ I F + + GA++ TYLLE++R
Sbjct: 189 KRSVENQVLATNPIMEAFGNAKTIRNDNSSRFGKYVTISFDENLLITGANVNTYLLERSR 248
Query: 875 VISQQALERSYHIFYQMMSG 934
V+S ER+YHIFYQ+++G
Sbjct: 249 VVSLLKGERNYHIFYQLITG 268
>SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr
2|||Manual
Length = 1217
Score = 173 bits (422), Expect = 2e-44
Identities = 83/199 (41%), Positives = 128/199 (64%)
Frame = +2
Query: 326 EDMSNLTYLNDASVLYNLKQRYYHKLIYTYSGLFCVAINPYKRFPVYTTRCAKLYRGKRR 505
+D++ L+ + D + NL+ R+ + IYTY G +++NP++ +YT K Y+GK R
Sbjct: 43 DDLTLLSKITDEEINKNLELRFRNGEIYTYIGHVLISVNPFRDLGIYTMDILKSYQGKNR 102
Query: 506 SEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASQKKDPS 685
E PH++AI++ AY M + HENQ ++I+GESGAGKTE K+++ Y V S +
Sbjct: 103 LETSPHVYAIAENAYYQMKSYHENQCIIISGESGAGKTEAAKRIMQYITHVSKSVGTEIE 162
Query: 686 QEKKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPSGKLAGADIETYLLE 865
+ + + ++ TNP+LE+FG AKT+RN+NSSR GK++ + F G GA I YLLE
Sbjct: 163 R-----VSEIILATNPLLESFGCAKTLRNNNSSRHGKYLEMIFNSGGVPVGAKITNYLLE 217
Query: 866 KARVISQQALERSYHIFYQ 922
K R+++Q ER++HIFYQ
Sbjct: 218 KNRIVNQVRNERNFHIFYQ 236
>SPBC16H5.10c |prp43||ATP-dependent RNA helicase
Prp43|Schizosaccharomyces pombe|chr 2|||Manual
Length = 735
Score = 30.3 bits (65), Expect = 0.44
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = +2
Query: 569 HENQSMLITGESGAGKTENTKKVIAY 646
HENQ ++ GE+G+GKT + + Y
Sbjct: 89 HENQIIVFVGETGSGKTTQIPQFVLY 114
>SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1489
Score = 27.9 bits (59), Expect = 2.3
Identities = 20/56 (35%), Positives = 29/56 (51%), Gaps = 4/56 (7%)
Frame = -1
Query: 958 DGFKTVDGAGHHLVEDVVRTLKSLLGDDTSL----LKQIGFDVSTSQFSGWTEMDT 803
D FK VDG+ L + TL S+L + S+ KQ+GF S+F ++ DT
Sbjct: 1048 DNFKLVDGSITALQRKNLYTLSSILSEIFSIESCDSKQLGFFRPLSEFIEVSKQDT 1103
>SPCC1919.03c |||AMP-activated protein kinase beta subunit
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 298
Score = 27.5 bits (58), Expect = 3.1
Identities = 17/51 (33%), Positives = 23/51 (45%)
Frame = +2
Query: 305 PPKYEKCEDMSNLTYLNDASVLYNLKQRYYHKLIYTYSGLFCVAINPYKRF 457
PP EKC SN Y D SVL N + L + L +A++ R+
Sbjct: 233 PPHLEKCILNSNTAYKEDQSVLPNPNHVLLNHLAAANTQLGVLALSATTRY 283
>SPAC3G6.01 |hrp3||ATP-dependent DNA helicase
Hrp3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1388
Score = 26.6 bits (56), Expect = 5.4
Identities = 10/28 (35%), Positives = 17/28 (60%)
Frame = +2
Query: 569 HENQSMLITGESGAGKTENTKKVIAYFA 652
H+N++ ++ E G GKT T ++Y A
Sbjct: 391 HKNENGILADEMGLGKTVQTVAFLSYLA 418
>SPAC23C4.02 |crn1||actin binding protein, coronin
Crn1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 601
Score = 26.6 bits (56), Expect = 5.4
Identities = 11/31 (35%), Positives = 18/31 (58%)
Frame = +2
Query: 653 TVGASQKKDPSQEKKGSLEDQVVQTNPVLEA 745
T KK+PS+EKK + D+ + P+ E+
Sbjct: 504 TFSEDVKKEPSEEKKLEVSDEAPKAAPLAES 534
>SPBPB21E7.07 |aes1|SPAPB21E7.07, SPAPB21E7.07|enhancer of
RNA-mediated gene silencing |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 296
Score = 26.2 bits (55), Expect = 7.2
Identities = 9/24 (37%), Positives = 14/24 (58%)
Frame = -2
Query: 846 MSAPASFPDGPKWIRMNLPKREEL 775
+S P DGPKW+ + LP ++
Sbjct: 156 VSPPVLIDDGPKWLVIQLPNATDV 179
>SPBC887.14c |pfh1|pif1|pif1 helicase homolog
Pfh1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 805
Score = 26.2 bits (55), Expect = 7.2
Identities = 26/107 (24%), Positives = 47/107 (43%), Gaps = 3/107 (2%)
Frame = +2
Query: 329 DMSNLTYLNDASVLYNLKQRYYHKLIYT--YSGLFCVAINPYKRFPVYTTRCAKLYR-GK 499
D SN +S L +L ++ K T S F V +N + P+ ++ L++
Sbjct: 244 DSSNKLSNGRSSSLDSLAKKRMSKSKSTPQISKKFSVPLNSASKSPIGSS----LFKTSD 299
Query: 500 RRSEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVI 640
R + P IF + + + + S+ TG +G GK+ +K+I
Sbjct: 300 SRKKSVPSIFLSDEQKRILDMVVEQQHSIFFTGSAGTGKSVLLRKII 346
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 25.8 bits (54), Expect = 9.5
Identities = 11/20 (55%), Positives = 12/20 (60%)
Frame = -2
Query: 108 YGVGSGSSPSWTTGFGIFGG 49
+G GSG P GFG FGG
Sbjct: 193 FGGGSGGPPPGPGGFGGFGG 212
>SPAC4G8.05 |ppk14||serine/threonine protein kinase Ppk14
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 566
Score = 25.8 bits (54), Expect = 9.5
Identities = 16/53 (30%), Positives = 27/53 (50%)
Frame = +2
Query: 677 DPSQEKKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPSGKLA 835
+ S+E + ++ED + + + G+ K S+F FIR+HF S K A
Sbjct: 8 ESSEEGRINVEDHLEEAKK--DDTGHWKHSGTAKPSKFRAFIRLHFKDSRKFA 58
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,073,765
Number of Sequences: 5004
Number of extensions: 86044
Number of successful extensions: 286
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 257
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 278
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 513276802
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -