Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV30026X
         (510 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch...    60   3e-10
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces...    38   7e-04
SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce...    37   0.002
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma...    32   0.043
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb...    29   0.40 
SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual    26   3.8  
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha...    25   5.0  
SPAC25G10.08 |||translation initiation factor eIF3b |Schizosacch...    25   6.6  
SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce...    25   6.6  
SPBC4.04c |mcm2|cdc19, nda1|MCM complex subunit Mcm2 |Schizosacc...    25   6.6  
SPCC594.06c |||SNARE Vam7 |Schizosaccharomyces pombe|chr 3|||Manual    25   6.6  
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch...    25   8.7  

>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 150

 Score = 59.7 bits (138), Expect = 3e-10
 Identities = 28/88 (31%), Positives = 51/88 (57%)
 Frame = -3

Query: 508 EFPEFVTLAAKFIVEEDAEAMQKELREAFRLYDKEGIGYIPTSSLREIXXXXXXXXXXXX 329
           +F EF+T+ A+ + + D E   +E+REAF+++DK+G GYI    L  +            
Sbjct: 66  DFTEFLTMMARKMKDTDNE---EEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEE 122

Query: 328 XXXLIQEIDTDGSGTVDFDEFMEMMTGE 245
              +I+E DTDG G ++++EF  +++ +
Sbjct: 123 VADMIREADTDGDGVINYEEFSRVISSK 150



 Score = 48.4 bits (110), Expect = 6e-07
 Identities = 23/62 (37%), Positives = 34/62 (54%)
 Frame = -3

Query: 439 ELREAFRLYDKEGIGYIPTSSLREIXXXXXXXXXXXXXXXLIQEIDTDGSGTVDFDEFME 260
           E REAF L+D++  G I ++ L  +               +I E+D DG+GT+DF EF+ 
Sbjct: 13  EFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFLT 72

Query: 259 MM 254
           MM
Sbjct: 73  MM 74


>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 143

 Score = 38.3 bits (85), Expect = 7e-04
 Identities = 20/80 (25%), Positives = 41/80 (51%)
 Frame = -3

Query: 499 EFVTLAAKFIVEEDAEAMQKELREAFRLYDKEGIGYIPTSSLREIXXXXXXXXXXXXXXX 320
           +F++  +  + E ++E   +E  +AFR++DK+  GYI T+   +                
Sbjct: 62  KFMSFVSNKLRETESE---EEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQL 118

Query: 319 LIQEIDTDGSGTVDFDEFME 260
           ++QE D   SG+ D+ +F++
Sbjct: 119 MVQEADPTNSGSFDYYDFVQ 138



 Score = 27.5 bits (58), Expect = 1.2
 Identities = 12/25 (48%), Positives = 17/25 (68%)
 Frame = -3

Query: 439 ELREAFRLYDKEGIGYIPTSSLREI 365
           E++EAF LYD +  G IPTS +  +
Sbjct: 10  EMKEAFVLYDIDKDGLIPTSHVGSV 34


>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 174

 Score = 37.1 bits (82), Expect = 0.002
 Identities = 24/92 (26%), Positives = 42/92 (45%), Gaps = 5/92 (5%)
 Frame = -3

Query: 508 EFPEFVTLAAKFIVEEDAEAMQKELREAFRLYDKEGIGYIPTSSL-----REIXXXXXXX 344
           +F EF+   + F V  + E   ++L+ AF++YD +  GYI    L       +       
Sbjct: 74  DFQEFINSLSVFSVHGNKE---EKLKFAFKIYDIDRDGYISNGELYLVLKMMVGTNLRED 130

Query: 343 XXXXXXXXLIQEIDTDGSGTVDFDEFMEMMTG 248
                    I E+D D  G + F+EF ++++G
Sbjct: 131 QLQQIVDKTIMEVDKDRDGKISFEEFKDIVSG 162



 Score = 26.2 bits (55), Expect = 2.9
 Identities = 9/19 (47%), Positives = 13/19 (68%)
 Frame = -3

Query: 307 IDTDGSGTVDFDEFMEMMT 251
           +D DG G VDF EF+  ++
Sbjct: 65  VDEDGGGDVDFQEFINSLS 83


>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 176

 Score = 32.3 bits (70), Expect = 0.043
 Identities = 16/71 (22%), Positives = 32/71 (45%)
 Frame = -3

Query: 463 EDAEAMQKELREAFRLYDKEGIGYIPTSSLREIXXXXXXXXXXXXXXXLIQEIDTDGSGT 284
           E  E  ++++ EAF+L+D +    I    LR                 ++++ D  G G 
Sbjct: 30  EITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGY 89

Query: 283 VDFDEFMEMMT 251
           +  ++F+ +MT
Sbjct: 90  LQMEDFVRVMT 100


>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 141

 Score = 29.1 bits (62), Expect = 0.40
 Identities = 15/60 (25%), Positives = 31/60 (51%)
 Frame = -3

Query: 433 REAFRLYDKEGIGYIPTSSLREIXXXXXXXXXXXXXXXLIQEIDTDGSGTVDFDEFMEMM 254
           ++AF L+D+ G G IP +S+ ++                I EI++     VD ++F++++
Sbjct: 9   KQAFSLFDRHGTGRIPKTSIGDL----LRACGQNPTLAEITEIESTLPAEVDMEQFLQVL 64


>SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1236

 Score = 25.8 bits (54), Expect = 3.8
 Identities = 15/36 (41%), Positives = 21/36 (58%)
 Frame = -1

Query: 372  ARSSGNWTSS*LTMNSMASSKRSTLTEAAPSTSTSS 265
            A ++G+ TSS  T +   SS  S  T +A  +STSS
Sbjct: 968  ASATGSSTSSIATASVSGSSTSSVATASATDSSTSS 1003



 Score = 25.4 bits (53), Expect = 5.0
 Identities = 16/38 (42%), Positives = 21/38 (55%)
 Frame = -1

Query: 378  ACARSSGNWTSS*LTMNSMASSKRSTLTEAAPSTSTSS 265
            A A  +G+ TSS  T +   SS  S  T +A  +STSS
Sbjct: 1005 AAASVTGSSTSSVATASVTDSSTSSVATASATDSSTSS 1042


>SPAC926.03 |rlc1||myosin II regulatory light chain
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 184

 Score = 25.4 bits (53), Expect = 5.0
 Identities = 11/18 (61%), Positives = 14/18 (77%)
 Frame = -3

Query: 442 KELREAFRLYDKEGIGYI 389
           +EL+EAF L DK+G G I
Sbjct: 48  QELKEAFALLDKDGDGNI 65


>SPAC25G10.08 |||translation initiation factor eIF3b
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 725

 Score = 25.0 bits (52), Expect = 6.6
 Identities = 12/36 (33%), Positives = 25/36 (69%)
 Frame = -3

Query: 472 IVEEDAEAMQKELREAFRLYDKEGIGYIPTSSLREI 365
           + +ED + ++K+L++  RL+D+E I    +S+ RE+
Sbjct: 630 LTKEDMKKIRKKLKDYNRLFDEEDIAE-QSSANREL 664


>SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1036

 Score = 25.0 bits (52), Expect = 6.6
 Identities = 16/57 (28%), Positives = 32/57 (56%)
 Frame = -1

Query: 435 SGKRSDCTTRKASVTFPRRACARSSGNWTSS*LTMNSMASSKRSTLTEAAPSTSTSS 265
           S   +  ++  +S T    + + +S + TSS  T +S+ASS  ++ + A+ S ++SS
Sbjct: 82  SSSLTSSSSLASSSTNSTTSASPTSSSLTSSSATSSSLASSSTTSSSLASSSITSSS 138


>SPBC4.04c |mcm2|cdc19, nda1|MCM complex subunit Mcm2
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 830

 Score = 25.0 bits (52), Expect = 6.6
 Identities = 15/34 (44%), Positives = 20/34 (58%)
 Frame = -3

Query: 502 PEFVTLAAKFIVEEDAEAMQKELREAFRLYDKEG 401
           PEF   AA+ +VEED E +     EA  + D+EG
Sbjct: 38  PEFSDEAAEALVEEDIEDLD---GEALDVEDEEG 68


>SPCC594.06c |||SNARE Vam7 |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 341

 Score = 25.0 bits (52), Expect = 6.6
 Identities = 12/40 (30%), Positives = 17/40 (42%)
 Frame = -1

Query: 381 RACARSSGNWTSS*LTMNSMASSKRSTLTEAAPSTSTSSW 262
           R C   S +W SS +T   +  S+R  L       S + W
Sbjct: 59  RLCKLPSKSWVSSTVTNEKLRESRRLALQAYVQCLSETPW 98


>SPAC1687.14c |||EF hand family protein, unknown
           role|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 76

 Score = 24.6 bits (51), Expect = 8.7
 Identities = 12/26 (46%), Positives = 14/26 (53%)
 Frame = -3

Query: 448 MQKELREAFRLYDKEGIGYIPTSSLR 371
           M +E  EAF L+D    GYI    LR
Sbjct: 10  MDEEAEEAFDLFDVTHKGYIDFEDLR 35


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,661,819
Number of Sequences: 5004
Number of extensions: 28137
Number of successful extensions: 106
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 91
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 103
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 204242806
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)