BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30010 (735 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC18.14c |rpp0||60S acidic ribosomal protein Rpp0 |Schizosacch... 248 8e-67 SPBC11G11.03 |||60S acidic ribosomal protein |Schizosaccharomyce... 62 6e-11 SPAC22E12.04 |ccs1|pccs, pccs|metallochaperone Ccs1 |Schizosacch... 29 0.91 SPAC823.13c |||mitochondrial inner membrane protein|Schizosaccha... 27 2.1 SPAC3H1.04c |mdm31||mitochondrial inner membrane protein Mdm31|S... 27 2.1 SPAC17G8.14c |pck1|SPAC22H10.01c|protein kinase C |Schizosacchar... 27 2.8 SPCC663.01c |ekc1|SPCC777.16c|protein phosphatase regulatory sub... 27 3.7 SPAC30.01c |sec72|sec7b|Sec7 domain|Schizosaccharomyces pombe|ch... 26 4.8 SPAC6B12.04c |||aminotransferase class I and II|Schizosaccharomy... 26 6.4 SPCC737.07c |||DNA polymerase alpha-associated DNA helicase A |S... 25 8.5 SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 25 8.5 SPBC337.15c |coq7||ubiquinone biosynthesis protein Coq7|Schizosa... 25 8.5 >SPCC18.14c |rpp0||60S acidic ribosomal protein Rpp0 |Schizosaccharomyces pombe|chr 3|||Manual Length = 312 Score = 248 bits (606), Expect = 8e-67 Identities = 116/217 (53%), Positives = 157/217 (72%) Frame = +3 Query: 84 KSNYFIKIIQLLDEYPKCFIVGADNVGSQQMQQIRXSLRGSSIVLMGKNTMMRKAIKDHL 263 K+ YF K+ L ++Y F+V DNV SQQM +R LRG++ ++MGKNTM+R+A++ + Sbjct: 8 KAQYFEKLRSLFEKYNSLFVVNIDNVSSQQMHTVRKQLRGTAELIMGKNTMIRRAMRGII 67 Query: 264 DNNPALEKLLPHIKGNVGLVFTRGDLVEVRDKLLENKVQAPARPGAIAPLSVVIPAHNTG 443 ++ P LE+LLP ++GNVG VFT DL EVR+ ++ N + APARP AIAPL V +PA NTG Sbjct: 68 NDMPELERLLPVVRGNVGFVFTNADLKEVRETIIANVIAAPARPNAIAPLDVFVPAGNTG 127 Query: 444 LGP*KTYFFQALSIPTKISKGTIEIINDVHILKPGDKVGASEATLLNMLNISPFSYGLVG 623 + P KT FFQAL IPTKI++GTIEI +DVH++ KVG SEATLLNMLNISPF+YG+ Sbjct: 128 MEPGKTSFFQALGIPTKITRGTIEITSDVHLVSKDAKVGPSEATLLNMLNISPFTYGMDV 187 Query: 624 KQVYDSGTIFAPEILDIKPEDLRAKFQAGVANVAALS 734 +YD G +F+PEILD+ EDL + + + A+S Sbjct: 188 LTIYDQGNVFSPEILDVSEEDLIGHLLSAASIITAIS 224 >SPBC11G11.03 |||60S acidic ribosomal protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 241 Score = 62.5 bits (145), Expect = 6e-11 Identities = 66/222 (29%), Positives = 105/222 (47%), Gaps = 21/222 (9%) Frame = +3 Query: 21 KFHRSPYATLSRMGREDKATWKSNYFIKIIQLLDEYPKCFIVGADNVGSQQMQQIRXSLR 200 K RS TL++ ++ K+ F + Q LD + +I N+ + +++IR + Sbjct: 3 KSRRSKVLTLAQTEKKGHEG-KAALFSGVQQSLDSFDYMWIFDVTNMRNTYLKRIRDDWK 61 Query: 201 GSSIVLMGKNTMMRKAI-----KDHLDNNPALEKLLPHIKGNVGLVFTRGDLVEVRDKLL 365 GS I MGK +M KA+ ++H +N L KLL G VGL+FT EV Sbjct: 62 GSRI-FMGKTKVMAKALGHTPEEEHAENVSKLTKLL---HGAVGLLFTNSKPDEVIG-YF 116 Query: 366 ENKVQAP-ARPGAIAPLSVVIPA----HNTGLGP*K-----TYFFQ----ALSIPTKISK 503 E+ VQ AR GA+AP + VIPA G P + T+ + L +PT + Sbjct: 117 ESFVQNDFARAGAVAPFTHVIPAGPVYSRAGQIPVEDDILLTHTLEPQVRQLGMPTVLKN 176 Query: 504 GTIEIINDVHILKPGDKVGASEATLLNMLNI--SPFSYGLVG 623 G + ++ D + G ++ + + LL + I + F GL+G Sbjct: 177 GVVTLLADFPLCTEGQQLDSRQTRLLKLFGITAAEFKVGLLG 218 >SPAC22E12.04 |ccs1|pccs, pccs|metallochaperone Ccs1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 297 Score = 28.7 bits (61), Expect = 0.91 Identities = 13/34 (38%), Positives = 21/34 (61%) Frame = +1 Query: 214 CSWEKTL*CAKPSKTTWTTIQPSRNCCHTSRATL 315 CS EKT C++ K+ T+ +PS CC ++T+ Sbjct: 264 CSTEKTSCCSQEKKSCCTSEKPS--CCSNGKSTV 295 >SPAC823.13c |||mitochondrial inner membrane protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 317 Score = 27.5 bits (58), Expect = 2.1 Identities = 10/28 (35%), Positives = 16/28 (57%) Frame = -3 Query: 280 RAGLLSRWSLMALRIIVFFPMSTILEPR 197 +A W LM + +++F + ILEPR Sbjct: 162 QASTWGTWGLMGINVVLFVVVQLILEPR 189 >SPAC3H1.04c |mdm31||mitochondrial inner membrane protein Mdm31|Schizosaccharomyces pombe|chr 1|||Manual Length = 601 Score = 27.5 bits (58), Expect = 2.1 Identities = 13/42 (30%), Positives = 24/42 (57%) Frame = -3 Query: 358 LSRTSTRSPRVNTKPTLPLMCGNSFSRAGLLSRWSLMALRII 233 L +T +S + PTLP + + S +G+LSR + + ++ I Sbjct: 35 LKQTVLQSSSFKSFPTLPRLAARNISNSGILSRTTPVIIKQI 76 >SPAC17G8.14c |pck1|SPAC22H10.01c|protein kinase C |Schizosaccharomyces pombe|chr 1|||Manual Length = 988 Score = 27.1 bits (57), Expect = 2.8 Identities = 15/36 (41%), Positives = 22/36 (61%), Gaps = 1/36 (2%) Frame = +1 Query: 433 TTPASVHRKPISSK-LFPSLPKFQRVLLKSSTMYTS 537 TT V+RKP+SS+ F +LP + LL ++ TS Sbjct: 562 TTNEKVYRKPLSSQNTFDTLPTISQGLLAATQPVTS 597 >SPCC663.01c |ekc1|SPCC777.16c|protein phosphatase regulatory subunit Ekc1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 838 Score = 26.6 bits (56), Expect = 3.7 Identities = 12/33 (36%), Positives = 18/33 (54%) Frame = +1 Query: 427 PPTTPASVHRKPISSKLFPSLPKFQRVLLKSST 525 PPTT ++ + +P FQ++LLK ST Sbjct: 309 PPTTRDPIYLGTMLRLFAEKIPVFQKILLKPST 341 >SPAC30.01c |sec72|sec7b|Sec7 domain|Schizosaccharomyces pombe|chr 1|||Manual Length = 1822 Score = 26.2 bits (55), Expect = 4.8 Identities = 16/54 (29%), Positives = 26/54 (48%) Frame = -3 Query: 373 LFSNSLSRTSTRSPRVNTKPTLPLMCGNSFSRAGLLSRWSLMALRIIVFFPMST 212 LFSN + R + R+ T L+ GN++ + W+L+A I F +T Sbjct: 1512 LFSNCICRDNITLSRIGTNCMQQLLSGNAYRFE--VKDWNLVADMFIELFKETT 1563 >SPAC6B12.04c |||aminotransferase class I and II|Schizosaccharomyces pombe|chr 1|||Manual Length = 421 Score = 25.8 bits (54), Expect = 6.4 Identities = 22/64 (34%), Positives = 28/64 (43%), Gaps = 3/64 (4%) Frame = -3 Query: 451 GPRPVLWAGMTTDNGAMAPGRAGAWTLFSNSLSRTSTRSPR---VNTKPTLPLMCGNSFS 281 G PV + + G++ P AGAW L N L T + +NT P PL G FS Sbjct: 139 GGVPVYVPIIPPEEGSVKPVSAGAWKLDMNKLRNAITEKTKMIVINT-PHNPL--GKIFS 195 Query: 280 RAGL 269 L Sbjct: 196 EEEL 199 >SPCC737.07c |||DNA polymerase alpha-associated DNA helicase A |Schizosaccharomyces pombe|chr 3|||Manual Length = 660 Score = 25.4 bits (53), Expect = 8.5 Identities = 15/46 (32%), Positives = 26/46 (56%) Frame = +3 Query: 210 IVLMGKNTMMRKAIKDHLDNNPALEKLLPHIKGNVGLVFTRGDLVE 347 I L+G N ++ DH+ +P ++ P+I LV ++GDLV+ Sbjct: 401 IPLLGMNKVILAG--DHMQLSPNVQSKRPYISMFERLVKSQGDLVK 444 >SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 583 Score = 25.4 bits (53), Expect = 8.5 Identities = 13/54 (24%), Positives = 28/54 (51%) Frame = +1 Query: 358 NCWRTKSRLQLVPVPLPHCQSSFPPTTPASVHRKPISSKLFPSLPKFQRVLLKS 519 + +R++ R L+ P +S PP TPA+ + ++P+ R++L++ Sbjct: 79 SAFRSRYRGSLLNRNSPSLRSLSPPATPATPRSRIEGESQTSAIPQTDRLILEN 132 >SPBC337.15c |coq7||ubiquinone biosynthesis protein Coq7|Schizosaccharomyces pombe|chr 2|||Manual Length = 216 Score = 25.4 bits (53), Expect = 8.5 Identities = 14/41 (34%), Positives = 21/41 (51%) Frame = -1 Query: 669 PEFQVQK*FQNHIPAYQQDHMRMERCSTC*EGWLQKLQPCH 547 PEF K ++H+ ++ D + E +T EGW K P H Sbjct: 160 PEF---KEIRSHLAEFRDDEL--EHLNTAVEGWNAKEAPAH 195 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,381,927 Number of Sequences: 5004 Number of extensions: 73995 Number of successful extensions: 210 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 201 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 210 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 347244562 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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