SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV30008
         (905 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyc...    31   0.23 
SPCC895.09c |ucp12||ATP-dependent RNA helicase Ucp1 |Schizosacch...    31   0.30 
SPBC17D1.06 |dbp3||ATP-dependent RNA helicase Dbp3 |Schizosaccha...    31   0.30 
SPAC10F6.09c |psm3|smc3|mitotic cohesin complex subunit Psm3|Sch...    30   0.52 
SPBC36.07 |iki3||RNA polymerase II elongator subunit Iki3 |Schiz...    29   0.91 
SPBC20F10.05 |||DuF1740 family protein|Schizosaccharomyces pombe...    29   0.91 
SPBC1703.14c |top1||DNA topoisomerase I|Schizosaccharomyces pomb...    29   0.91 
SPAC6G10.02c |tea3||cell end marker Tea3|Schizosaccharomyces pom...    28   1.6  
SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces...    28   1.6  
SPBC713.09 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    28   2.1  
SPAC3H8.09c |nab3||poly|Schizosaccharomyces pombe|chr 1|||Manual       28   2.1  
SPAC4F8.04 |||Brix domain protein Rpf1|Schizosaccharomyces pombe...    27   2.8  
SPBC1105.04c |cbp1|abp1|CENP-B homolog|Schizosaccharomyces pombe...    27   2.8  
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac...    27   3.7  
SPBC3E7.13c |||splicing factor, SYF2 family|Schizosaccharomyces ...    27   3.7  
SPBC25B2.05 |mis3||rRNA processing protein Mis3|Schizosaccharomy...    27   4.8  
SPAC4F8.02c |mrpl40|SPAC644.02|mitochondrial ribosomal protein s...    26   6.4  
SPCC594.05c |||COMPASS complex subunit |Schizosaccharomyces pomb...    26   8.5  
SPAC3A11.14c |pkl1|klp1, SPAC3H5.03c|kinesin-like protein Pkl1|S...    26   8.5  
SPAC6G9.06c |pcp1||pericentrin Pcp1|Schizosaccharomyces pombe|ch...    26   8.5  

>SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1085

 Score = 31.1 bits (67), Expect = 0.23
 Identities = 27/119 (22%), Positives = 57/119 (47%), Gaps = 5/119 (4%)
 Frame = -3

Query: 687 KRVCKDYHERIARLEDEKF--DLEYIVKRK-DMEISDLNSQVNDLRGKFVKPTLKKVSKY 517
           +RV + Y E++A+    K   ++E   KRK + E+  +  +      K      K+  + 
Sbjct: 554 QRVLQAYREKVAQQRQAKLLEEIEEENKRKQERELKKIREKEKKRDKKKQLKLAKEEERQ 613

Query: 516 ENKFAKLQKKAAE--FNFRNQLKVVKKKEFTLEEEDKEKKPDWSKGKPGDQKVKEEEVE 346
             +  +L ++AA+     + Q +  KK+E    + ++EKK    + +  ++K K++E E
Sbjct: 614 RREAERLAEQAAQKALEAKRQEEARKKREEQRLKREQEKKQQELERQKREEKQKQKERE 672


>SPCC895.09c |ucp12||ATP-dependent RNA helicase Ucp1
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1327

 Score = 30.7 bits (66), Expect = 0.30
 Identities = 15/65 (23%), Positives = 31/65 (47%)
 Frame = -3

Query: 543 PTLKKVSKYENKFAKLQKKAAEFNFRNQLKVVKKKEFTLEEEDKEKKPDWSKGKPGDQKV 364
           PT KK + ++ K  +  ++ +E    N  + VK   + L+  ++EK P W   +     +
Sbjct: 531 PTAKKNNSFKPKLFRRSRELSEKLCNNWSERVKSPSYQLKVREREKLPAWESRRKIMDAI 590

Query: 363 KEEEV 349
           +  +V
Sbjct: 591 QHSQV 595


>SPBC17D1.06 |dbp3||ATP-dependent RNA helicase Dbp3
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 578

 Score = 30.7 bits (66), Expect = 0.30
 Identities = 17/70 (24%), Positives = 36/70 (51%)
 Frame = -3

Query: 555 KFVKPTLKKVSKYENKFAKLQKKAAEFNFRNQLKVVKKKEFTLEEEDKEKKPDWSKGKPG 376
           K  K +++++ +  ++ A +++K  +    ++     KK+    ++D+  K D  K K G
Sbjct: 49  KMAKRSVEELKRSADEEASVKRKEKKSKHEHKKH---KKDKPSADKDRISKKDKKKSKKG 105

Query: 375 DQKVKEEEVE 346
             K KEE +E
Sbjct: 106 KSKTKEESIE 115


>SPAC10F6.09c |psm3|smc3|mitotic cohesin complex subunit
            Psm3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1194

 Score = 29.9 bits (64), Expect = 0.52
 Identities = 14/56 (25%), Positives = 29/56 (51%)
 Frame = -3

Query: 729  GKPKNIDDANEDTIKRVCKDYHERIARLEDEKFDLEYIVKRKDMEISDLNSQVNDL 562
            G    ID++  +++KR    Y  ++  ++     LE  ++R + EISD  +++  L
Sbjct: 837  GSDNRIDESELNSVKRSLLKYENKLQIIKSSSSGLEEQMQRINSEISDKRNELESL 892


>SPBC36.07 |iki3||RNA polymerase II elongator subunit Iki3
            |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1253

 Score = 29.1 bits (62), Expect = 0.91
 Identities = 13/35 (37%), Positives = 19/35 (54%)
 Frame = -3

Query: 474  NFRNQLKVVKKKEFTLEEEDKEKKPDWSKGKPGDQ 370
            +FRNQ+K   ++   L E+ KE    W +G   DQ
Sbjct: 1070 DFRNQIKSQTERILVLREKKKEDPISWMEGTMEDQ 1104


>SPBC20F10.05 |||DuF1740 family protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 972

 Score = 29.1 bits (62), Expect = 0.91
 Identities = 19/68 (27%), Positives = 34/68 (50%), Gaps = 3/68 (4%)
 Frame = -3

Query: 669 YHERIARLEDEKFDLEYIVKRKDMEISDLNS---QVNDLRGKFVKPTLKKVSKYENKFAK 499
           +H+ +   +DE     Y++  K ME  +L+    QVN +  K +K     VS + N +A+
Sbjct: 578 FHQLVFSADDEYLASVYLIYLKQMETKNLSEEKPQVNKIVKKILKKYDSSVSVW-NTYAQ 636

Query: 498 LQKKAAEF 475
           L+  +  F
Sbjct: 637 LEHLSGAF 644


>SPBC1703.14c |top1||DNA topoisomerase I|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 814

 Score = 29.1 bits (62), Expect = 0.91
 Identities = 18/59 (30%), Positives = 29/59 (49%), Gaps = 2/59 (3%)
 Frame = -3

Query: 516 ENKFAKLQKKAAEFNFRNQLKVVKKKEFTLEEEDKEKKPDWSKGKPGDQK--VKEEEVE 346
           +N F    K   E NF + +K   K +FT      E+K +  K  P +QK  +K+++ E
Sbjct: 254 DNFFRDFLKVCDECNFNHNIKEFSKCDFTQMFHHFEQKREEKKSMPKEQKKAIKQKKDE 312


>SPAC6G10.02c |tea3||cell end marker Tea3|Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1125

 Score = 28.3 bits (60), Expect = 1.6
 Identities = 16/58 (27%), Positives = 32/58 (55%)
 Frame = -3

Query: 687  KRVCKDYHERIARLEDEKFDLEYIVKRKDMEISDLNSQVNDLRGKFVKPTLKKVSKYE 514
            +R+C+   + +   + + +DL++       E S L+ QVNDL  +  K T +K+S+ +
Sbjct: 897  QRLCEGRTKELLNSQQKLYDLKHSYSSVMTEKSKLSDQVNDLTEQ-AKITQRKLSEVQ 953


>SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces
            pombe|chr 3|||Manual
          Length = 1526

 Score = 28.3 bits (60), Expect = 1.6
 Identities = 21/88 (23%), Positives = 46/88 (52%), Gaps = 1/88 (1%)
 Frame = -3

Query: 609  RKDMEISDLNSQVNDLRGKFVKPTLKKVS-KYENKFAKLQKKAAEFNFRNQLKVVKKKEF 433
            R ++E  +L  QVN+L+G  V P     S ++ N    ++ KA     ++Q+   K K F
Sbjct: 1423 RLNLEKHNLEKQVNELKGAEVSPQPTGQSLQHVNLAHAIELKA----LKDQINSEKAKMF 1478

Query: 432  TLEEEDKEKKPDWSKGKPGDQKVKEEEV 349
            +++ + ++++ +  K     +KV ++ +
Sbjct: 1479 SVQVQYEKREQELQKRIASLEKVNKDSL 1506


>SPBC713.09 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 395

 Score = 27.9 bits (59), Expect = 2.1
 Identities = 28/103 (27%), Positives = 50/103 (48%), Gaps = 2/103 (1%)
 Frame = -3

Query: 648 LEDEKFDLEYIVKRKDMEISDLNSQVNDLRGKFVKPTLK-KVSKYENKFAKLQKK-AAEF 475
           LE +   L  + ++KD +I +L  ++NDL   +   TLK      E K   + K+ AA  
Sbjct: 149 LEKQVKTLHDLNEQKDKKIKELKERINDLTYDY--ETLKANADDSEGKQTLVSKREAALE 206

Query: 474 NFRNQLKVVKKKEFTLEEEDKEKKPDWSKGKPGDQKVKEEEVE 346
            F+++L ++++ E    E     K D  K +  D + +  +VE
Sbjct: 207 EFQSKL-LIRENEINKRELKMNGKEDDLKKREKDLENRLLKVE 248


>SPAC3H8.09c |nab3||poly|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 738

 Score = 27.9 bits (59), Expect = 2.1
 Identities = 16/71 (22%), Positives = 33/71 (46%), Gaps = 1/71 (1%)
 Frame = -3

Query: 576 QVNDLRGKFVKPTLKKVSK-YENKFAKLQKKAAEFNFRNQLKVVKKKEFTLEEEDKEKKP 400
           Q N +RG+ +   + K+ K Y+N+   ++K +         +++    +      + + P
Sbjct: 418 QGNFVRGQKLHLEISKIQKKYQNQIENMKKGSHVTKSNQYSEMIGNLPYPTSSRKRTRSP 477

Query: 399 DWSKGKPGDQK 367
             SKGK  D+K
Sbjct: 478 LMSKGKSYDRK 488


>SPAC4F8.04 |||Brix domain protein Rpf1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 306

 Score = 27.5 bits (58), Expect = 2.8
 Identities = 15/43 (34%), Positives = 25/43 (58%), Gaps = 1/43 (2%)
 Frame = -3

Query: 528 VSKYENKFAKLQKKAAEFNFRNQLKVVKKKEFTLEEE-DKEKK 403
           + K +NK  + Q+     + +N+ K+ ++KE   EEE D EKK
Sbjct: 1   MGKIKNKIVRQQQYMKALHQKNKDKLERRKERAKEEEKDPEKK 43


>SPBC1105.04c |cbp1|abp1|CENP-B homolog|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 522

 Score = 27.5 bits (58), Expect = 2.8
 Identities = 13/40 (32%), Positives = 22/40 (55%)
 Frame = -3

Query: 720 KNIDDANEDTIKRVCKDYHERIARLEDEKFDLEYIVKRKD 601
           +NI D++ED I ++   Y +  A   DE+   E+ +  KD
Sbjct: 435 ENIVDSSEDIINQIASQYMDDRAFETDEEESTEFQITTKD 474


>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
            Spp42|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 2363

 Score = 27.1 bits (57), Expect = 3.7
 Identities = 19/79 (24%), Positives = 38/79 (48%), Gaps = 1/79 (1%)
 Frame = -3

Query: 612  KRKDMEISDLNSQVNDLRGKFVKPTLKK-VSKYENKFAKLQKKAAEFNFRNQLKVVKKKE 436
            K+ D  I+   S +    G+ + P L + +  +E++F   Q+  AE+  + Q  + + + 
Sbjct: 1368 KQTDTGITHFRSGMTT-NGEHLIPNLYRYIQPWESEFIDSQRVWAEYAMKRQEALQQNRR 1426

Query: 435  FTLEEEDKEKKPDWSKGKP 379
             TLE    + +  W +G P
Sbjct: 1427 LTLE----DLEDSWDRGIP 1441


>SPBC3E7.13c |||splicing factor, SYF2 family|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 229

 Score = 27.1 bits (57), Expect = 3.7
 Identities = 30/127 (23%), Positives = 58/127 (45%), Gaps = 9/127 (7%)
 Frame = -3

Query: 714 IDDANEDTIKRVCKDYHERIARLEDEKFDLEYIVKRK-DMEISDLNSQVNDLRGKFVKPT 538
           ID A E  ++R   +  E +A++E E+   ++  +R  D  I +       LR K  K T
Sbjct: 42  IDPALERRLERKRMEAEEELAKIETEEEGGDFERRRAWDWTIEESEKWDQRLRKK--KQT 99

Query: 537 LKKV--SKYENKFAK-----LQKKAAEFN-FRNQLKVVKKKEFTLEEEDKEKKPDWSKGK 382
           ++ V  S Y ++ +K     ++    ++  +    K  K K  ++   D+ ++ DW   K
Sbjct: 100 IQNVAFSDYHSQASKDYNRGIRDLKPDYEKYDKDKKEEKSKSRSVALYDEAERLDWVSNK 159

Query: 381 PGDQKVK 361
           P  + V+
Sbjct: 160 PDKEHVE 166


>SPBC25B2.05 |mis3||rRNA processing protein Mis3|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 327

 Score = 26.6 bits (56), Expect = 4.8
 Identities = 12/35 (34%), Positives = 22/35 (62%)
 Frame = -3

Query: 450 VKKKEFTLEEEDKEKKPDWSKGKPGDQKVKEEEVE 346
           ++  E+ L++E+KE+K    K +   +K KE+E E
Sbjct: 275 IESGEYFLKKEEKERKKRAEKKEQQKEKKKEKEKE 309


>SPAC4F8.02c |mrpl40|SPAC644.02|mitochondrial ribosomal protein
           subunit L40|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 279

 Score = 26.2 bits (55), Expect = 6.4
 Identities = 12/41 (29%), Positives = 20/41 (48%)
 Frame = -3

Query: 492 KKAAEFNFRNQLKVVKKKEFTLEEEDKEKKPDWSKGKPGDQ 370
           KK    + R +++    K     ++DK  KP+   GKP D+
Sbjct: 22  KKPLPLHMRTKIREHLNKSDPTVKDDKSAKPELPFGKPADR 62


>SPCC594.05c |||COMPASS complex subunit |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 424

 Score = 25.8 bits (54), Expect = 8.5
 Identities = 13/44 (29%), Positives = 22/44 (50%)
 Frame = -3

Query: 699 EDTIKRVCKDYHERIARLEDEKFDLEYIVKRKDMEISDLNSQVN 568
           ED+ +    D+H   A  E+EK     + K   ME+ ++N  V+
Sbjct: 23  EDSNRGTITDFHIETANNEEEKDANVILNKSVKMEVEEVNGHVD 66


>SPAC3A11.14c |pkl1|klp1, SPAC3H5.03c|kinesin-like protein
           Pkl1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 832

 Score = 25.8 bits (54), Expect = 8.5
 Identities = 12/41 (29%), Positives = 22/41 (53%)
 Frame = +1

Query: 622 FQIEFFIFKTSDAFMVVFANPLNSIFVGVVNVLRFTAPLLD 744
           + +++ + K +   M V  +PL S F+  +N LRF   + D
Sbjct: 781 YLLKYSLGKGAKTLMFVNVSPLKSQFMDTLNSLRFATKVND 821


>SPAC6G9.06c |pcp1||pericentrin Pcp1|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1208

 Score = 25.8 bits (54), Expect = 8.5
 Identities = 11/33 (33%), Positives = 21/33 (63%)
 Frame = -3

Query: 669 YHERIARLEDEKFDLEYIVKRKDMEISDLNSQV 571
           +HE I +L+D + +L   +++K++ IS L   V
Sbjct: 599 FHESINKLQDREKELTSNLEKKNLVISSLRETV 631


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,146,723
Number of Sequences: 5004
Number of extensions: 61395
Number of successful extensions: 232
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 221
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 231
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 458501510
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -