Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0948
(633 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC2G11.10c |||URM1 activating enzyme |Schizosaccharomyces pomb... 111 1e-25
SPAC24H6.12c |uba3||NEDD8 activating enzyme|Schizosaccharomyces ... 63 4e-11
SPBC16H5.03c |fub2|uba2|SUMO E1-like activator enzyme Fub2|Schiz... 62 5e-11
SPBC1604.21c |ptr3|uba1, SPBC211.09|ubiquitin activating enzyme ... 43 4e-05
SPAC1A6.10 ||SPAC30D11.15c|Moeb/ThiF domain|Schizosaccharomyces ... 41 1e-04
SPBC6B1.05c |||ubiquitin-like conjugating enzyme|Schizosaccharom... 40 4e-04
SPAC323.06c |uba5||NEDD8 activating enzyme |Schizosaccharomyces ... 36 0.005
SPCC663.12 |cid12||poly|Schizosaccharomyces pombe|chr 3|||Manual 26 5.2
SPCC23B6.01c |||oxysterol binding protein |Schizosaccharomyces p... 25 9.1
SPAC20G8.04c |||mitochondrial electron transfer flavoprotein-ubi... 25 9.1
>SPAC2G11.10c |||URM1 activating enzyme |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 401
Score = 111 bits (266), Expect = 1e-25
Identities = 56/122 (45%), Positives = 78/122 (63%), Gaps = 1/122 (0%)
Frame = +2
Query: 269 RYSRQILLSDIGVEGQVKICSAKVLIXGAGGLGCPAAMYLAGAGIGEIGIVDYDAVDLTN 448
RY RQ+LLS+IG+ GQ+ + + VL+ GAGGLGCPA YL AGIG +GI+D D VD +N
Sbjct: 23 RYGRQMLLSEIGLPGQLSLKRSSVLVIGAGGLGCPAMQYLVAAGIGTLGIMDGDVVDKSN 82
Query: 449 VHRQLLHHESNENTSKAFSALS-L*DV*TRKLKSHHTTFSWTRRTLSKFASAYDLVLDCS 625
+HRQ++H S + KA SA L D+ + + + F+ + L YD+VLDC+
Sbjct: 83 LHRQIIHSTSKQGMHKAISAKQFLEDLNPNVIINTYLEFA-SASNLFSIIEQYDVVLDCT 141
Query: 626 DN 631
DN
Sbjct: 142 DN 143
>SPAC24H6.12c |uba3||NEDD8 activating enzyme|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 444
Score = 62.9 bits (146), Expect = 4e-11
Identities = 29/63 (46%), Positives = 43/63 (68%)
Frame = +2
Query: 329 SAKVLIXGAGGLGCPAAMYLAGAGIGEIGIVDYDAVDLTNVHRQLLHHESNENTSKAFSA 508
S+K+LI GAGGLGC LA +G ++ ++D D +D+TN++RQ L +ESN + KA A
Sbjct: 44 SSKILIIGAGGLGCEILKDLALSGFRDLSVIDMDTIDITNLNRQFLFNESNIDEPKANVA 103
Query: 509 LSL 517
S+
Sbjct: 104 ASM 106
>SPBC16H5.03c |fub2|uba2|SUMO E1-like activator enzyme
Fub2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 628
Score = 62.5 bits (145), Expect = 5e-11
Identities = 38/111 (34%), Positives = 57/111 (51%), Gaps = 2/111 (1%)
Frame = +2
Query: 305 VEGQVKICSAKVLIXGAGGLGCPAAMYLAGAGIGEIGIVDYDAVDLTNVHRQLLHHESNE 484
VE SAKVL+ GAGG+GC L +G+ E+ I+D D +DL+N++RQ L + +
Sbjct: 17 VEALRNFKSAKVLLVGAGGIGCELLKNLLMSGVKEVHIIDLDTIDLSNLNRQFLFRKKHV 76
Query: 485 NTSKAFSALSL*DV*TR--KLKSHHTTFSWTRRTLSKFASAYDLVLDCSDN 631
KA A KL+++H R ++ F +DLV + DN
Sbjct: 77 KQPKAIVAAKTASSFNPNVKLEAYHANIKEDRFNVAWFRQ-FDLVFNALDN 126
>SPBC1604.21c |ptr3|uba1, SPBC211.09|ubiquitin activating enzyme
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1012
Score = 42.7 bits (96), Expect = 4e-05
Identities = 26/71 (36%), Positives = 37/71 (52%), Gaps = 5/71 (7%)
Frame = +2
Query: 269 RYSRQILLSDIGVEGQVKICSAKVLIXGAGGLGCP-----AAMYLAGAGIGEIGIVDYDA 433
RY QI + G E Q KI S + GAG +GC A M +A G I + D D+
Sbjct: 409 RYDGQIAV--FGSEFQEKIASLSTFLVGAGAIGCEMLKNWAMMGVATGESGHISVTDMDS 466
Query: 434 VDLTNVHRQLL 466
++ +N++RQ L
Sbjct: 467 IEKSNLNRQFL 477
Score = 31.1 bits (67), Expect = 0.14
Identities = 20/76 (26%), Positives = 35/76 (46%)
Frame = +2
Query: 272 YSRQILLSDIGVEGQVKICSAKVLIXGAGGLGCPAAMYLAGAGIGEIGIVDYDAVDLTNV 451
YSRQ+ + +G E ++ + VLI G GLG A + AG+ + + D + ++
Sbjct: 20 YSRQLYV--LGHEAMKQMSQSNVLIIGCKGLGVEIAKNVCLAGVKSVTLYDPQPTRIEDL 77
Query: 452 HRQLLHHESNENTSKA 499
Q E + +A
Sbjct: 78 SSQYFLTEDDIGVPRA 93
>SPAC1A6.10 ||SPAC30D11.15c|Moeb/ThiF domain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 485
Score = 41.1 bits (92), Expect = 1e-04
Identities = 20/68 (29%), Positives = 39/68 (57%)
Frame = +2
Query: 302 GVEGQVKICSAKVLIXGAGGLGCPAAMYLAGAGIGEIGIVDYDAVDLTNVHRQLLHHESN 481
G +G ++ ++ V++ G GG+G LA +G+ +I IVD+D V L++++R + +
Sbjct: 117 GEDGMERLRNSFVIVVGCGGVGSWVINMLARSGVQKIRIVDFDQVSLSSLNRHSIATLQD 176
Query: 482 ENTSKAFS 505
T K +
Sbjct: 177 VGTPKTLA 184
>SPBC6B1.05c |||ubiquitin-like conjugating
enzyme|Schizosaccharomyces pombe|chr 2|||Manual
Length = 649
Score = 39.5 bits (88), Expect = 4e-04
Identities = 20/49 (40%), Positives = 27/49 (55%)
Frame = +2
Query: 320 KICSAKVLIXGAGGLGCPAAMYLAGAGIGEIGIVDYDAVDLTNVHRQLL 466
+I ++K L+ GAG LGC A L G+ + VDY V +N RQ L
Sbjct: 332 RIQNSKCLLLGAGTLGCGVARNLLSWGVRHVTFVDYSTVSYSNPVRQSL 380
>SPAC323.06c |uba5||NEDD8 activating enzyme |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 500
Score = 35.9 bits (79), Expect = 0.005
Identities = 26/85 (30%), Positives = 37/85 (43%)
Frame = +2
Query: 263 IERYSRQILLSDIGVEGQVKICSAKVLIXGAGGLGCPAAMYLAGAGIGEIGIVDYDAVDL 442
+++Y RQ+ L EGQ I + V + A +GC A L GIG +VD +VD
Sbjct: 7 MQKYDRQVRLWK--AEGQNAIEKSHVCLLYANTVGCEALKNLILPGIGSFAVVDDTSVDF 64
Query: 443 TNVHRQLLHHESNENTSKAFSALSL 517
+ E S+A SL
Sbjct: 65 SMDGMNFFIQYDQEGKSRARCTASL 89
>SPCC663.12 |cid12||poly|Schizosaccharomyces pombe|chr 3|||Manual
Length = 336
Score = 25.8 bits (54), Expect = 5.2
Identities = 9/23 (39%), Positives = 15/23 (65%)
Frame = -1
Query: 264 IAHLEDRHLLMILKHHLSADCDY 196
+ LE R + M+L+ +L AD D+
Sbjct: 87 VGELEKRRVTMVLRKYLDADADF 109
>SPCC23B6.01c |||oxysterol binding protein |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 489
Score = 25.0 bits (52), Expect = 9.1
Identities = 10/20 (50%), Positives = 12/20 (60%)
Frame = -3
Query: 421 HDAYFANARPGKVHGSRTSQ 362
HD Y +ARP + G R SQ
Sbjct: 296 HDVYLVSARPLEEQGERESQ 315
>SPAC20G8.04c |||mitochondrial electron transfer
flavoprotein-ubiquinone
oxidoreductase|Schizosaccharomyces pombe|chr 1|||Manual
Length = 632
Score = 25.0 bits (52), Expect = 9.1
Identities = 8/12 (66%), Positives = 11/12 (91%)
Frame = -1
Query: 159 FLIPEVHFPFPL 124
FLIP++HFP P+
Sbjct: 171 FLIPKLHFPIPV 182
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,396,169
Number of Sequences: 5004
Number of extensions: 46124
Number of successful extensions: 128
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 121
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 127
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 281707720
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -