Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0943
(618 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 176 2e-45
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 95 7e-21
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 94 2e-20
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 88 1e-18
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 60 3e-10
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 54 2e-08
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 44 1e-05
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 31 0.18
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 27 2.9
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 27 2.9
SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 3.8
SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual 26 5.0
SPAC637.07 |moe1||translation initiation factor eIF3d Moe1|Schiz... 25 6.6
SPAC6G10.10c |||human hmmtag2 homolog|Schizosaccharomyces pombe|... 25 8.8
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 176 bits (428), Expect = 2e-45
Identities = 78/82 (95%), Positives = 80/82 (97%)
Frame = +2
Query: 257 MGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLT 436
MGQKDSYVGDEAQSKRGILTLKYPIEHGIV NWDDMEKIWHHTFYNELRVAPEEHP LLT
Sbjct: 47 MGQKDSYVGDEAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLT 106
Query: 437 EAPLNPKANREKMTQIMFETFN 502
EAPLNPK+NREKMTQI+FETFN
Sbjct: 107 EAPLNPKSNREKMTQIIFETFN 128
Score = 91.5 bits (217), Expect = 9e-20
Identities = 39/44 (88%), Positives = 43/44 (97%)
Frame = +3
Query: 123 DEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMV 254
+EE+AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MV
Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMV 45
Score = 68.5 bits (160), Expect = 7e-13
Identities = 34/38 (89%), Positives = 36/38 (94%), Gaps = 1/38 (2%)
Frame = +1
Query: 502 HAP-MYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPI 612
+AP YVAIQAVLSLYASGRTTGIVLDSGDGV+HTVPI
Sbjct: 128 NAPAFYVAIQAVLSLYASGRTTGIVLDSGDGVTHTVPI 165
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 95.1 bits (226), Expect = 7e-21
Identities = 38/85 (44%), Positives = 62/85 (72%)
Frame = +2
Query: 245 RDGRMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHP 424
+ G + KD VGDEA++ R +L +KYP+E+GI+ ++++M ++W +TF+ +L++ P
Sbjct: 43 KTGNVQIKDVMVGDEAEAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRK 102
Query: 425 VLLTEAPLNPKANREKMTQIMFETF 499
+LLTE P+NP ANREKM + MFE +
Sbjct: 103 ILLTEPPMNPVANREKMCETMFERY 127
Score = 56.4 bits (130), Expect = 3e-09
Identities = 24/34 (70%), Positives = 31/34 (91%)
Frame = +1
Query: 511 MYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPI 612
+YVAIQAVLSLYA G ++G+V+DSGDGV+H VP+
Sbjct: 132 VYVAIQAVLSLYAQGLSSGVVVDSGDGVTHIVPV 165
Score = 48.0 bits (109), Expect = 1e-06
Identities = 21/35 (60%), Positives = 26/35 (74%)
Frame = +3
Query: 129 EVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP 233
E A +V+DNG+G K G+A D+ PR FPSIVGRP
Sbjct: 2 ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRP 36
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 93.9 bits (223), Expect = 2e-20
Identities = 41/81 (50%), Positives = 57/81 (70%), Gaps = 1/81 (1%)
Frame = +2
Query: 263 QKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYN-ELRVAPEEHPVLLTE 439
QKD +VG EAQ+ RG+L ++ PIE GI+ NW DME+IW + + + +L PEEHP+LLTE
Sbjct: 53 QKDMFVGSEAQNLRGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTE 112
Query: 440 APLNPKANREKMTQIMFETFN 502
PL N+EK+ + +ET N
Sbjct: 113 PPLANIRNKEKIAEYFYETLN 133
Score = 55.2 bits (127), Expect = 7e-09
Identities = 22/37 (59%), Positives = 29/37 (78%)
Frame = +3
Query: 141 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVM 251
+ +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM
Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVM 48
Score = 49.6 bits (113), Expect = 4e-07
Identities = 21/31 (67%), Positives = 28/31 (90%)
Frame = +1
Query: 520 AIQAVLSLYASGRTTGIVLDSGDGVSHTVPI 612
++Q VL+LYAS RTTGIVL+ GDG++H+VPI
Sbjct: 140 SLQPVLALYASARTTGIVLECGDGLTHSVPI 170
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 87.8 bits (208), Expect = 1e-18
Identities = 38/79 (48%), Positives = 50/79 (63%), Gaps = 1/79 (1%)
Frame = +2
Query: 269 DSYVGDEAQSKRGI-LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAP 445
D ++G++A K +L YPI HG + NWD ME+ W + + LR PE+H LLTE P
Sbjct: 73 DFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPP 132
Query: 446 LNPKANREKMTQIMFETFN 502
LNP NRE +IMFE+FN
Sbjct: 133 LNPPENRENTAEIMFESFN 151
Score = 40.7 bits (91), Expect = 2e-04
Identities = 14/28 (50%), Positives = 23/28 (82%)
Frame = +3
Query: 141 LVVDNGSGMCKAGFAGDDAPRAVFPSIV 224
+++DNG+G K G+AG+DAP VFP+++
Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVI 35
Score = 40.3 bits (90), Expect = 2e-04
Identities = 21/44 (47%), Positives = 30/44 (68%), Gaps = 8/44 (18%)
Frame = +1
Query: 505 APMYVAIQAVLSLYASGRT--------TGIVLDSGDGVSHTVPI 612
A +Y+A+QAVL+L AS + TG V+DSGDGV+H +P+
Sbjct: 153 AGLYIAVQAVLALAASWTSSKVTDRSLTGTVVDSGDGVTHIIPV 196
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 60.1 bits (139), Expect = 3e-10
Identities = 28/87 (32%), Positives = 52/87 (59%), Gaps = 1/87 (1%)
Frame = +2
Query: 245 RDGRMGQKDSYVGDEAQSKRGILTL-KYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEH 421
RD ++ + + VG++ + G ++ + P E +++NWD ME++ +TF +L + EH
Sbjct: 58 RDRKLSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEH 116
Query: 422 PVLLTEAPLNPKANREKMTQIMFETFN 502
P+ +TE NP R MT+++FE +N
Sbjct: 117 PICMTEPLANPTYVRSTMTELLFELYN 143
Score = 35.9 bits (79), Expect = 0.005
Identities = 15/32 (46%), Positives = 22/32 (68%)
Frame = +3
Query: 141 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 236
LV+DNGS +AG+ G+ P+ VF ++V R R
Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58
Score = 27.1 bits (57), Expect = 2.2
Identities = 12/33 (36%), Positives = 21/33 (63%), Gaps = 2/33 (6%)
Frame = +1
Query: 523 IQAVLSLYASGR--TTGIVLDSGDGVSHTVPIL 615
I + S Y + + ++GIVL+ G+ SH +P+L
Sbjct: 151 IDGLFSFYHNTKPSSSGIVLNLGNAASHVIPVL 183
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 54.0 bits (124), Expect = 2e-08
Identities = 24/62 (38%), Positives = 35/62 (56%)
Frame = +2
Query: 311 LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMF 490
+ +K I +G V NWD +W + +L+ P EHP+L+TE NP NR K + MF
Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMF 122
Query: 491 ET 496
E+
Sbjct: 123 ES 124
Score = 40.3 bits (90), Expect = 2e-04
Identities = 16/34 (47%), Positives = 24/34 (70%)
Frame = +3
Query: 126 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 227
+EV+A+V+D GS + GF+G+D P+ V PS G
Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG 42
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 44.4 bits (100), Expect = 1e-05
Identities = 22/78 (28%), Positives = 42/78 (53%), Gaps = 2/78 (2%)
Frame = +2
Query: 266 KDSYVGDEAQSKRGI--LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTE 439
++ YV DE Q I + +K +GI+ +W+ W +L+V P E+ +++TE
Sbjct: 49 RNKYVVDELQIHAPIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITE 108
Query: 440 APLNPKANREKMTQIMFE 493
NP++ R+++ + FE
Sbjct: 109 PSWNPQSVRQQIMEAAFE 126
Score = 36.7 bits (81), Expect = 0.003
Identities = 14/34 (41%), Positives = 23/34 (67%)
Frame = +3
Query: 126 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 227
EE+ +LV+D GS + G+AG+++P + PS G
Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPSYYG 41
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 30.7 bits (66), Expect = 0.18
Identities = 19/60 (31%), Positives = 28/60 (46%), Gaps = 4/60 (6%)
Frame = +2
Query: 326 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 493
PI+ G V +W+ ++ W H Y+ L P + +PV L +RE TQ FE
Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171
Score = 25.8 bits (54), Expect = 5.0
Identities = 12/33 (36%), Positives = 19/33 (57%)
Frame = +1
Query: 517 VAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIL 615
+A + ++ LYA G G+V+D G + PIL
Sbjct: 180 IAYEPLMGLYAIGILHGLVIDIGYEKTDITPIL 212
>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1666
Score = 26.6 bits (56), Expect = 2.9
Identities = 13/41 (31%), Positives = 24/41 (58%), Gaps = 2/41 (4%)
Frame = +2
Query: 386 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFN 502
+Y L E+HP+LLT+ A L P+ + ++ +I ++ N
Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSEN 1439
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 26.6 bits (56), Expect = 2.9
Identities = 12/32 (37%), Positives = 18/32 (56%), Gaps = 1/32 (3%)
Frame = +3
Query: 171 KAGFAGDDAP-RAVFPSIVGRPRHQGVMVVWD 263
KA F+G R+ + VG+P H G++ WD
Sbjct: 1533 KAKFSGQKGTLRSKYSLEVGKPMHYGIISEWD 1564
>SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 226
Score = 26.2 bits (55), Expect = 3.8
Identities = 10/25 (40%), Positives = 18/25 (72%)
Frame = +2
Query: 428 LLTEAPLNPKANREKMTQIMFETFN 502
+L APL+ + +KM +++FET+N
Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205
>SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1828
Score = 25.8 bits (54), Expect = 5.0
Identities = 12/23 (52%), Positives = 14/23 (60%)
Frame = -1
Query: 168 TYRSHCLLPTRQLLRRTS*ISEI 100
TYR H +PT LL T +SEI
Sbjct: 168 TYRDHDAIPTATLLNPTLDVSEI 190
>SPAC637.07 |moe1||translation initiation factor eIF3d
Moe1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 567
Score = 25.4 bits (53), Expect = 6.6
Identities = 16/40 (40%), Positives = 17/40 (42%)
Frame = -1
Query: 609 GHGVGDTVAGVQHDTGGTTGRVQREHGLDGDVHGGVLNVS 490
G G GDTV GG GR R D D H + N S
Sbjct: 139 GRGAGDTVVSRSSGAGGARGR--RFGWKDYDKHQRLRNAS 176
>SPAC6G10.10c |||human hmmtag2 homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 194
Score = 25.0 bits (52), Expect = 8.8
Identities = 18/40 (45%), Positives = 21/40 (52%)
Frame = +3
Query: 498 STRPHVRRHPSRALAVRVRSYHRYRAGLRRRCLPHRAHLT 617
ST P RH R R RS HR+ G RR P+R HL+
Sbjct: 128 STSP-ANRH-RRKEKERTRSNHRH--GSHRRHEPYRTHLS 163
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,803,943
Number of Sequences: 5004
Number of extensions: 60403
Number of successful extensions: 165
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 138
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 162
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 271646730
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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