Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0918
(676 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16E9.16c |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 35 0.012
SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyce... 34 0.016
SPBC646.09c |int6|yin6|translation initiation factor eIF3e|Schiz... 30 0.27
SPAC27F1.04c |nuf2||spindle pole body protein Nuf2|Schizosacchar... 30 0.35
SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos... 30 0.35
SPAC11E3.01c |swr1|SPAC2H10.03c|SNF2 family helicase Swr1|Schizo... 29 0.81
SPBC4F6.17c |||mitochondrial matrix chaperone Hsp78 |Schizosacch... 28 1.1
SPAC12B10.10 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 28 1.1
SPBC29A10.13 |atp7||F0-ATPase subunit D|Schizosaccharomyces pomb... 28 1.4
SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 27 1.9
SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces pomb... 27 1.9
SPAC32A11.04c |tif212|tif22, SPAC6B12.17c|translation initiation... 27 2.5
SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces... 27 3.3
SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces po... 27 3.3
SPCC2H8.05c ||SPCC63.01c|sequence orphan|Schizosaccharomyces pom... 27 3.3
SPBC16A3.19 |||histone acetyltransferase complex subunit Eaf7 |S... 27 3.3
SPBC1734.07c |||TRAPP complex subunit Trs85 |Schizosaccharomyces... 26 5.7
SPBC649.05 |cut12|stf1|spindle pole body protein Cut12 |Schizosa... 25 7.6
SPCC188.08c |ubp22|ubp5|ubiquitin C-terminal hydrolase Ubp22|Sch... 25 7.6
SPAPB21F2.03 |||ribosome biogenesis protein |Schizosaccharomyces... 25 10.0
SPCC18B5.01c |bfr1|hba2, SPCPJ732.04c|brefeldin A efflux transpo... 25 10.0
SPAC3A12.06c |||sodium/calcium exchanger |Schizosaccharomyces po... 25 10.0
SPAC17C9.11c |||zinc finger protein, zf-C2H2 type/UBA domain pro... 25 10.0
SPAC10F6.10 |||protein kinase, RIO family |Schizosaccharomyces p... 25 10.0
SPBC27B12.12c |||CorA family magnesium ion transporter |Schizosa... 25 10.0
SPAC144.17c |||6-phosphofructo-2-kinase|Schizosaccharomyces pomb... 25 10.0
>SPBC16E9.16c |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 642
Score = 34.7 bits (76), Expect = 0.012
Identities = 30/97 (30%), Positives = 52/97 (53%), Gaps = 2/97 (2%)
Frame = +3
Query: 144 IEHHTKEFHKTLEQQFNSLTKSKDAQD-FSKAWKGRLRSVLQQLNAFAKSLQGALGDANG 320
I+ K LEQ+ +L ++++A++ +A + RS + L A++ + A DA
Sbjct: 98 IQPDEKTLQDLLEQRQVALREAREAEEELQRARQYNDRSTSEALELEARAKKAAQ-DAE- 155
Query: 321 KAKEALEQSRQNIERTAEELRK-AHPDVEKNATALRE 428
A E +++ +IER+A K A + E+ ATALRE
Sbjct: 156 LASERAREAQSSIERSASLREKQAREEAERAATALRE 192
>SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 238
Score = 34.3 bits (75), Expect = 0.016
Identities = 28/107 (26%), Positives = 54/107 (50%), Gaps = 6/107 (5%)
Frame = +3
Query: 174 TLEQQFNSLTKSKDAQD---FSKAWKGRLRSVLQQLNAFAKSLQGALGDANGKAKEALEQ 344
T+E ++SL KSK + F + + + +L N + + L + +A KA+++LEQ
Sbjct: 8 TIELDYDSL-KSKISNFNSIFDRFLQEERKKLLNNKNEYLRQLS-EINEAQKKAEKSLEQ 65
Query: 345 SRQNIERTAEELRKAHPD---VEKNATALREKLQAAVQNTVQESQKL 476
+ + E L K H + E+ + +EKL A ++ + S++L
Sbjct: 66 TEARKQNFTELLEKEHEEQAITEQEIFSFQEKLDAMLKRKQKLSEEL 112
>SPBC646.09c |int6|yin6|translation initiation factor
eIF3e|Schizosaccharomyces pombe|chr 2|||Manual
Length = 501
Score = 30.3 bits (65), Expect = 0.27
Identities = 19/54 (35%), Positives = 30/54 (55%), Gaps = 2/54 (3%)
Frame = +3
Query: 219 QDFSKAWKGRLRSVLQQLNAFAKSLQGALGD-ANGKAKEALEQSR-QNIERTAE 374
+D + A+ + RSVLQ+L+ + +QG LG N AL Q + QN++ E
Sbjct: 79 EDMANAFAEKRRSVLQELSELEEEVQGILGVLENPDLIAALRQDKGQNLQHLQE 132
>SPAC27F1.04c |nuf2||spindle pole body protein
Nuf2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 441
Score = 29.9 bits (64), Expect = 0.35
Identities = 26/104 (25%), Positives = 47/104 (45%), Gaps = 13/104 (12%)
Frame = +3
Query: 246 RLRSVLQQLNAFAKSLQGALGDANGK---AKEALEQSRQNIERTAEELRKAHPDVEK-NA 413
R +L+QL+ + L+ N K AK+ ++ R+ + +E K + EK NA
Sbjct: 332 RKEQLLKQLSNAQEKLEHEQHSRNQKLEAAKQRMDNIREEYKVITQERNKKIQETEKKNA 391
Query: 414 ---------TALREKLQAAVQNTVQESQKLAKKVSSNVQETMRN 518
+RE+L++ + + E +KL V + E +RN
Sbjct: 392 MIEMTEQKIAGMREELESQISSITMEFEKLKSHVELYIAELLRN 435
>SPAC22F8.11 |plc1||phosphoinositide phospholipase C
Plc1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 899
Score = 29.9 bits (64), Expect = 0.35
Identities = 19/70 (27%), Positives = 33/70 (47%), Gaps = 3/70 (4%)
Frame = +3
Query: 114 RRDAPDFFKDIEHHTKEFHKTLEQQFNSLT---KSKDAQDFSKAWKGRLRSVLQQLNAFA 284
++ DFFK + ++ H TL ++ NSL+ +K + G L + +LN
Sbjct: 56 KKSEQDFFKMLSSRDRDAHSTLRKRSNSLSSFLSTKSTSASENKFHGGLNWLSLKLNLLL 115
Query: 285 KSLQGALGDA 314
+ LQG + A
Sbjct: 116 R-LQGRMNSA 124
>SPAC11E3.01c |swr1|SPAC2H10.03c|SNF2 family helicase
Swr1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1288
Score = 28.7 bits (61), Expect = 0.81
Identities = 24/80 (30%), Positives = 34/80 (42%)
Frame = +3
Query: 231 KAWKGRLRSVLQQLNAFAKSLQGALGDANGKAKEALEQSRQNIERTAEELRKAHPDVEKN 410
K R R + + A K L GA KAKE ++ R +RTA E+RK +E+
Sbjct: 137 KVRASRSRRISGMILAHFKRLSGA---DEKKAKEEDKRIRLLAKRTAWEIRKKWKVIERE 193
Query: 411 ATALREKLQAAVQNTVQESQ 470
R + A Q + Q
Sbjct: 194 VRRRRAERAAEAQRVAGKEQ 213
>SPBC4F6.17c |||mitochondrial matrix chaperone Hsp78
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 803
Score = 28.3 bits (60), Expect = 1.1
Identities = 24/74 (32%), Positives = 33/74 (44%), Gaps = 6/74 (8%)
Frame = +3
Query: 174 TLEQQFNSLTKSKDAQDFSKAWKGRLRSVLQQLNAFAKSLQGA------LGDANGKAKEA 335
T++ + SL K D + + +L S L L L A L D+ KAK
Sbjct: 354 TIQIELESLRKETDTTSVER--REKLESKLTDLKEEQDKLSAAWEEERKLLDSIKKAKTE 411
Query: 336 LEQSRQNIERTAEE 377
LEQ+R +ERT E
Sbjct: 412 LEQARIELERTQRE 425
>SPAC12B10.10 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 419
Score = 28.3 bits (60), Expect = 1.1
Identities = 16/78 (20%), Positives = 36/78 (46%)
Frame = +3
Query: 273 NAFAKSLQGALGDANGKAKEALEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQN 452
N + ++ AL + KA + LE+ ++ E + EE+ H + T+ + + +
Sbjct: 337 NLVSLAIYEALYEKFLKACKDLEEVSKSYEESREEIEALHETFTEEVTSFQSTKRLKEEK 396
Query: 453 TVQESQKLAKKVSSNVQE 506
+QE ++ K + Q+
Sbjct: 397 IIQEKSRVDKMIDEYRQK 414
>SPBC29A10.13 |atp7||F0-ATPase subunit D|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 175
Score = 27.9 bits (59), Expect = 1.4
Identities = 22/63 (34%), Positives = 37/63 (58%), Gaps = 10/63 (15%)
Frame = +3
Query: 249 LRSVLQQLNAF-AKSLQGALGDAN-GKAK--------EALEQSRQNIERTAEELRKAHPD 398
L S L+ +NAF AK+ +GA + KA+ + +EQ+R E T E++++A P+
Sbjct: 87 LNSQLKAINAFEAKASEGAKKNVELVKAELQNLSATLKNIEQARPTEEITIEDMKQAVPE 146
Query: 399 VEK 407
+EK
Sbjct: 147 IEK 149
>SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 412
Score = 27.5 bits (58), Expect = 1.9
Identities = 26/109 (23%), Positives = 47/109 (43%)
Frame = +3
Query: 93 LAQGAMVRRDAPDFFKDIEHHTKEFHKTLEQQFNSLTKSKDAQDFSKAWKGRLRSVLQQL 272
L + V R F + +EH+ K+LE+Q + L +SKDA A S+
Sbjct: 193 LKKSKSVPRLRGQFMEPVEHN-HPLSKSLEEQSSFLEQSKDASSNLTACNRSGSSLSSNF 251
Query: 273 NAFAKSLQGALGDANGKAKEALEQSRQNIERTAEELRKAHPDVEKNATA 419
+ S + +L N K++ +L+ ++ R P+V + +A
Sbjct: 252 YSSRLSKKTSLASLN-KSRASLQHKIMSLSRNIIRRVFHKPEVHLDPSA 299
>SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 631
Score = 27.5 bits (58), Expect = 1.9
Identities = 10/20 (50%), Positives = 13/20 (65%)
Frame = -2
Query: 351 ASTVPKPPWPCRSRLRALPG 292
AST+ K PWP + L +PG
Sbjct: 38 ASTLEKEPWPASTALLVMPG 57
>SPAC32A11.04c |tif212|tif22, SPAC6B12.17c|translation initiation
factor eIF2 beta subunit|Schizosaccharomyces pombe|chr
1|||Manual
Length = 321
Score = 27.1 bits (57), Expect = 2.5
Identities = 16/54 (29%), Positives = 26/54 (48%), Gaps = 1/54 (1%)
Frame = +3
Query: 156 TKEFHKTLEQQFNSLTKSKDAQDFSKAWKGRLRSVLQQLNAFAKSLQ-GALGDA 314
+K+ + E+Q +T DFS K + + L+AF K L+ + GDA
Sbjct: 89 SKKSSASAEEQTEDITTESGELDFSSMKKKKKKKKSADLSAFEKELEASSTGDA 142
>SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1485
Score = 26.6 bits (56), Expect = 3.3
Identities = 12/42 (28%), Positives = 19/42 (45%), Gaps = 2/42 (4%)
Frame = +3
Query: 120 DAPDFFKDIEHHTKEFHKTLEQ--QFNSLTKSKDAQDFSKAW 239
D +F D++ H K FH E+ + + +K D K W
Sbjct: 665 DMKSYFSDLDRHMKYFHAMQEKDAELIEMAFAKKKADVRKEW 706
>SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 670
Score = 26.6 bits (56), Expect = 3.3
Identities = 17/57 (29%), Positives = 26/57 (45%), Gaps = 5/57 (8%)
Frame = +3
Query: 288 SLQGALGDANGKAKEALEQSRQNIE-----RTAEELRKAHPDVEKNATALREKLQAA 443
S++ L + N + KE +E + RT +E EKN LRE+L+ A
Sbjct: 520 SMKDDLTEMNQRLKEQIESYENEVNSEITSRTLKEFETLKTQYEKNLCNLREQLKTA 576
>SPCC2H8.05c ||SPCC63.01c|sequence orphan|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 217
Score = 26.6 bits (56), Expect = 3.3
Identities = 12/33 (36%), Positives = 19/33 (57%)
Frame = +3
Query: 420 LREKLQAAVQNTVQESQKLAKKVSSNVQETMRN 518
L ++LQ Q+T+Q + L SSN +E + N
Sbjct: 51 LWDRLQPKAQSTIQRTSSLPVPSSSNFKERLNN 83
>SPBC16A3.19 |||histone acetyltransferase complex subunit Eaf7
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 272
Score = 26.6 bits (56), Expect = 3.3
Identities = 17/66 (25%), Positives = 36/66 (54%)
Frame = +3
Query: 321 KAKEALEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESQKLAKKVSSNV 500
K+++ LE S Q +E E + P+V++ +EK ++ V+ +E +K+S N+
Sbjct: 132 KSEKPLETS-QKVEIETVETKPGEPEVKQETNLQKEKKESKVKLESKE-----EKISRNL 185
Query: 501 QETMRN 518
+ + R+
Sbjct: 186 RSSSRS 191
>SPBC1734.07c |||TRAPP complex subunit Trs85 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 618
Score = 25.8 bits (54), Expect = 5.7
Identities = 12/18 (66%), Positives = 12/18 (66%)
Frame = -3
Query: 269 LLQHGSEPSFPSLAEVLC 216
L H SE SFPSL VLC
Sbjct: 119 LESHLSEASFPSLRSVLC 136
>SPBC649.05 |cut12|stf1|spindle pole body protein Cut12
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 548
Score = 25.4 bits (53), Expect = 7.6
Identities = 10/29 (34%), Positives = 18/29 (62%)
Frame = +3
Query: 378 LRKAHPDVEKNATALREKLQAAVQNTVQE 464
L AH D+E T++RE+L++ + +E
Sbjct: 267 LDSAHSDLELELTSIRERLESLILEKQEE 295
>SPCC188.08c |ubp22|ubp5|ubiquitin C-terminal hydrolase
Ubp22|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1108
Score = 25.4 bits (53), Expect = 7.6
Identities = 13/32 (40%), Positives = 19/32 (59%)
Frame = +3
Query: 315 NGKAKEALEQSRQNIERTAEELRKAHPDVEKN 410
N K KE E++ + EELR+A PD E++
Sbjct: 22 NEKLKEDFEENVSIDVKIHEELRRALPDYEES 53
>SPAPB21F2.03 |||ribosome biogenesis protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 172
Score = 25.0 bits (52), Expect = 10.0
Identities = 9/63 (14%), Positives = 31/63 (49%)
Frame = +3
Query: 327 KEALEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESQKLAKKVSSNVQE 506
K ++R+N++ + + P+++ + + +L+ +++++ Q + + V+E
Sbjct: 81 KRRNRKARENLKVNVSSIGEVLPEIDLDISVANSRLKPVIKDSLSSKQTKSSMKRNTVEE 140
Query: 507 TMR 515
R
Sbjct: 141 IER 143
>SPCC18B5.01c |bfr1|hba2, SPCPJ732.04c|brefeldin A efflux
transporter Bfr1|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1530
Score = 25.0 bits (52), Expect = 10.0
Identities = 13/26 (50%), Positives = 16/26 (61%)
Frame = -1
Query: 430 FSRRAVAFFSTSGWALRSSSAVRSMF 353
FSR V FFS AL+S S + +MF
Sbjct: 570 FSRGGVLFFSILFCALQSLSEIANMF 595
>SPAC3A12.06c |||sodium/calcium exchanger |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 743
Score = 25.0 bits (52), Expect = 10.0
Identities = 17/47 (36%), Positives = 25/47 (53%)
Frame = -3
Query: 368 RALDVLPRLFQSLLGLAVRVSERSLETLGEGVELLQHGSEPSFPSLA 228
RAL V+ L +S+LGL V + SL L + + + G FP +A
Sbjct: 608 RALGVIFNLNESILGLTVFAAGNSLSDLIADIMIARSG----FPEMA 650
>SPAC17C9.11c |||zinc finger protein, zf-C2H2 type/UBA domain
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 240
Score = 25.0 bits (52), Expect = 10.0
Identities = 14/63 (22%), Positives = 32/63 (50%), Gaps = 1/63 (1%)
Frame = +3
Query: 330 EALEQSRQNIE-RTAEELRKAHPDVEKNATALREKLQAAVQNTVQESQKLAKKVSSNVQE 506
+ E++ + I+ R+ EEL++A + + A +EK + + + K+ +K + +
Sbjct: 28 DQFEETEEEIKKRSPEELKQAIEALREKAKEKKEKERILALEEKKTNYKILQKSNDETAQ 87
Query: 507 TMR 515
MR
Sbjct: 88 AMR 90
>SPAC10F6.10 |||protein kinase, RIO family |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 521
Score = 25.0 bits (52), Expect = 10.0
Identities = 10/24 (41%), Positives = 15/24 (62%)
Frame = +3
Query: 306 GDANGKAKEALEQSRQNIERTAEE 377
G NG+AKE E+ R ++T E+
Sbjct: 473 GKGNGRAKETPEEKRARKKKTKED 496
>SPBC27B12.12c |||CorA family magnesium ion transporter
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 803
Score = 25.0 bits (52), Expect = 10.0
Identities = 10/24 (41%), Positives = 14/24 (58%)
Frame = -2
Query: 357 CSASTVPKPPWPCRSRLRALPGDS 286
C +T+PKPP+ + L LP S
Sbjct: 290 CPHTTIPKPPYQSDTDLTELPTKS 313
>SPAC144.17c |||6-phosphofructo-2-kinase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 432
Score = 25.0 bits (52), Expect = 10.0
Identities = 13/51 (25%), Positives = 26/51 (50%)
Frame = +3
Query: 342 QSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESQKLAKKVSS 494
++RQ E A+ +RK+ PDV + ++ +V + +E + +SS
Sbjct: 284 ENRQFSEYVADRIRKSFPDVSFKNLHVLSCMEDSVMSPFRELGSVTSSMSS 334
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,080,512
Number of Sequences: 5004
Number of extensions: 34935
Number of successful extensions: 198
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 188
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 197
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 309878492
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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