Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0873
(628 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 55 7e-09
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 50 4e-07
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 45 8e-06
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 38 0.002
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 36 0.005
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 34 0.015
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 30 0.31
SPBC16C6.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 29 0.55
SPCC23B6.03c |tel1||ATM checkpoint kinase|Schizosaccharomyces po... 27 1.7
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 27 2.2
SPBC32H8.08c |||mannosyltransferase complex subunit |Schizosacch... 27 2.2
SPBC354.10 |||RNAPII degradation factor |Schizosaccharomyces pom... 27 2.9
SPBC2A9.04c |||sir antagonist ortholog |Schizosaccharomyces pomb... 26 3.9
SPBC1271.14 |||glutamate N-acetyltransferase |Schizosaccharomyce... 26 5.1
SPBC3D6.05 |ptp4||phosphatidate cytidylyltransferase Ptp4 |Schiz... 25 6.8
SPAC16E8.14c |||methyltransferase |Schizosaccharomyces pombe|chr... 25 9.0
>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 492
Score = 55.2 bits (127), Expect = 7e-09
Identities = 31/77 (40%), Positives = 45/77 (58%), Gaps = 4/77 (5%)
Frame = +3
Query: 30 QAIVEFMRDPV---AQPKQKKKDVVDESWARDTDVVHLNGDSFDAILA-KAEHALVVFYA 197
+A+ +F+ D V QPK K + + + D+V L D+FD I+ + + LV FYA
Sbjct: 327 KAMTKFVGDFVDGKLQPKIKSQPIPESQ----EDLVVLVADNFDDIVMDETKDVLVEFYA 382
Query: 198 PWCGHCKRIKPEFEKAA 248
PWCGHCK + P +EK A
Sbjct: 383 PWCGHCKNLAPTYEKLA 399
Score = 49.2 bits (112), Expect = 5e-07
Identities = 18/43 (41%), Positives = 28/43 (65%)
Frame = +3
Query: 120 DVVHLNGDSFDAILAKAEHALVVFYAPWCGHCKRIKPEFEKAA 248
+V +N + + ++ + +V FYAPWCGHCK + PE+E AA
Sbjct: 23 EVPKVNKEGLNELITADKVLMVKFYAPWCGHCKALAPEYESAA 65
Score = 42.3 bits (95), Expect = 6e-05
Identities = 19/56 (33%), Positives = 30/56 (53%)
Frame = +2
Query: 263 EKINGILAAVDATKEPDLASRFGVKGYPTLKYFNKGEFKFDAGHARQEDQIISFIK 430
EK L VD T+E DL S + ++GYPTL F G+ R+ D ++ +++
Sbjct: 69 EKDGISLVEVDCTEEGDLCSEYSIRGYPTLNVFKNGKQISQYSGPRKHDALVKYMR 124
Score = 31.9 bits (69), Expect = 0.078
Identities = 11/24 (45%), Positives = 16/24 (66%)
Frame = +1
Query: 514 DFQKHTPEKSKHSIVMFYAPWCGH 585
+F +++K +V FYAPWCGH
Sbjct: 364 NFDDIVMDETKDVLVEFYAPWCGH 387
Score = 29.1 bits (62), Expect = 0.55
Identities = 9/11 (81%), Positives = 10/11 (90%)
Frame = +1
Query: 553 IVMFYAPWCGH 585
+V FYAPWCGH
Sbjct: 43 MVKFYAPWCGH 53
>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 363
Score = 49.6 bits (113), Expect = 4e-07
Identities = 18/45 (40%), Positives = 30/45 (66%)
Frame = +3
Query: 117 TDVVHLNGDSFDAILAKAEHALVVFYAPWCGHCKRIKPEFEKAAT 251
++ + LN +F + +LVVFYAPWCG+CK++ P ++K A+
Sbjct: 31 SNTIELNSKNFRKFVKAKGPSLVVFYAPWCGYCKKLVPTYQKLAS 75
Score = 32.7 bits (71), Expect = 0.045
Identities = 13/27 (48%), Positives = 19/27 (70%)
Frame = +1
Query: 514 DFQKHTPEKSKHSIVMFYAPWCGHLQK 594
+F+K K S+V+FYAPWCG+ +K
Sbjct: 40 NFRKFVKAKGP-SLVVFYAPWCGYCKK 65
>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 359
Score = 45.2 bits (102), Expect = 8e-06
Identities = 25/80 (31%), Positives = 39/80 (48%), Gaps = 1/80 (1%)
Frame = +3
Query: 3 FPYNGENKRQAIVEFMRDPVAQPKQKKKDVVDESWARDTDVVHLNGDSFD-AILAKAEHA 179
FP +G Q D + Q +K + ++VV L+ +FD ++ +
Sbjct: 102 FPPDGSEPVQYSNARDVDSLTQFVSEKTGIKKRKIVLPSNVVELDSLNFDKVVMDDKKDV 161
Query: 180 LVVFYAPWCGHCKRIKPEFE 239
LV FYA WCG+CKR+ P +E
Sbjct: 162 LVEFYADWCGYCKRLAPTYE 181
Score = 41.1 bits (92), Expect = 1e-04
Identities = 15/31 (48%), Positives = 21/31 (67%)
Frame = +3
Query: 150 DAILAKAEHALVVFYAPWCGHCKRIKPEFEK 242
+ I A + AL+ FYA WCGHCK + P +E+
Sbjct: 33 NTIRASKKGALIEFYATWCGHCKSLAPVYEE 63
Score = 32.3 bits (70), Expect = 0.059
Identities = 14/53 (26%), Positives = 27/53 (50%), Gaps = 1/53 (1%)
Frame = +2
Query: 278 ILAAVDATKEPDLASRFGVKGYPTLKYF-NKGEFKFDAGHARQEDQIISFIKD 433
++ +DA D+A ++ + G+PTL +F G +AR D + F+ +
Sbjct: 75 LIGKIDADTHSDVADKYHITGFPTLIWFPPDGSEPVQYSNARDVDSLTQFVSE 127
Score = 29.9 bits (64), Expect = 0.31
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = +1
Query: 511 LDFQKHTPEKSKHSIVMFYAPWCGHLQK 594
L+F K + K +V FYA WCG+ ++
Sbjct: 148 LNFDKVVMDDKKDVLVEFYADWCGYCKR 175
Score = 27.5 bits (58), Expect = 1.7
Identities = 9/27 (33%), Positives = 15/27 (55%)
Frame = +1
Query: 505 SILDFQKHTPEKSKHSIVMFYAPWCGH 585
S+ + + K +++ FYA WCGH
Sbjct: 27 SLNELENTIRASKKGALIEFYATWCGH 53
>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 103
Score = 37.5 bits (83), Expect = 0.002
Identities = 14/36 (38%), Positives = 23/36 (63%)
Frame = +3
Query: 135 NGDSFDAILAKAEHALVVFYAPWCGHCKRIKPEFEK 242
+ F +I+ + + +V F+A WCG CK I P+FE+
Sbjct: 7 DSSEFKSIVCQDKLVVVDFFATWCGPCKAIAPKFEQ 42
>SPBC12D12.07c |trx2||mitochondrial thioredoxin
Trx2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 121
Score = 35.9 bits (79), Expect = 0.005
Identities = 17/44 (38%), Positives = 25/44 (56%)
Frame = +3
Query: 111 RDTDVVHLNGDSFDAILAKAEHALVVFYAPWCGHCKRIKPEFEK 242
R + V GD ++ ++ + +V FYA WCG CK +KP EK
Sbjct: 17 RKVNAVESFGD-YNTRISADKVTVVDFYADWCGPCKYLKPFLEK 59
Score = 29.1 bits (62), Expect = 0.55
Identities = 14/41 (34%), Positives = 21/41 (51%)
Frame = +2
Query: 251 KVKTEKINGILAAVDATKEPDLASRFGVKGYPTLKYFNKGE 373
K+ + AV+A K D+A + GV PT+ F KG+
Sbjct: 59 KLSEQNQKASFIAVNADKFSDIAQKNGVYALPTMVLFRKGQ 99
>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
Txl1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 290
Score = 34.3 bits (75), Expect = 0.015
Identities = 14/32 (43%), Positives = 19/32 (59%)
Frame = +3
Query: 159 LAKAEHALVVFYAPWCGHCKRIKPEFEKAATK 254
+ K+ + V YA WCG CK I P F + A+K
Sbjct: 16 IPKSGYLAVDCYADWCGPCKAISPLFSQLASK 47
Score = 27.9 bits (59), Expect = 1.3
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = +2
Query: 278 ILAAVDATKEPDLASRFGVKGYPTLKYFNKGE 373
+ A V+ ++ +AS GVK PT +F G+
Sbjct: 54 VFAKVNVDEQRQIASGLGVKAMPTFVFFENGK 85
>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 29.9 bits (64), Expect = 0.31
Identities = 12/38 (31%), Positives = 21/38 (55%), Gaps = 2/38 (5%)
Frame = +3
Query: 141 DSFDAILAKAEHALVV--FYAPWCGHCKRIKPEFEKAA 248
+ F IL + +++ FYAPW CK++ F++ A
Sbjct: 9 EQFQEILQNGKEQIILLNFYAPWAAPCKQMNQVFDQFA 46
>SPBC16C6.04 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 350
Score = 29.1 bits (62), Expect = 0.55
Identities = 13/31 (41%), Positives = 18/31 (58%)
Frame = +3
Query: 477 PRKSLPVRHLDPRLSETHSGKIQTQHCHVLR 569
P K + V++L S +HSG+I C VLR
Sbjct: 120 PNKHIEVKNLKDLCSPSHSGRISKSLCPVLR 150
>SPCC23B6.03c |tel1||ATM checkpoint kinase|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 2812
Score = 27.5 bits (58), Expect = 1.7
Identities = 10/28 (35%), Positives = 16/28 (57%)
Frame = -3
Query: 245 RFFELWFNTFAVSTPGRVEYDQCVFRLC 162
+FFE +F + T + QC+F+LC
Sbjct: 230 KFFEFFFYAYTEETDAHLPALQCLFQLC 257
>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 726
Score = 27.1 bits (57), Expect = 2.2
Identities = 9/24 (37%), Positives = 14/24 (58%)
Frame = +3
Query: 183 VVFYAPWCGHCKRIKPEFEKAATK 254
+ +Y P CG CKR+ P ++ K
Sbjct: 47 IKYYLPSCGACKRLGPMWDNMVEK 70
>SPBC32H8.08c |||mannosyltransferase complex subunit
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 438
Score = 27.1 bits (57), Expect = 2.2
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = +3
Query: 69 PKQKKKDVVDESWARDTDVVHLNGDSF 149
PK KD++DES A VV+ N S+
Sbjct: 173 PKDVDKDMIDESIAEQVGVVYANFPSY 199
>SPBC354.10 |||RNAPII degradation factor |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 963
Score = 26.6 bits (56), Expect = 2.9
Identities = 13/32 (40%), Positives = 16/32 (50%)
Frame = +2
Query: 509 SSTFRNTLRKNPNTALSCSTRHGAATCKSTKP 604
SSTFRN RK+ + S T G K+ P
Sbjct: 149 SSTFRNATRKSKKPSASKDTSRGVRKSKAGAP 180
>SPBC2A9.04c |||sir antagonist ortholog |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 741
Score = 26.2 bits (55), Expect = 3.9
Identities = 10/22 (45%), Positives = 15/22 (68%)
Frame = -2
Query: 339 PLTPNLDAKSGSFVASTAANIP 274
P+T NL + SGS ST+ ++P
Sbjct: 314 PMTSNLTSNSGSMTNSTSTDLP 335
>SPBC1271.14 |||glutamate N-acetyltransferase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 445
Score = 25.8 bits (54), Expect = 5.1
Identities = 15/49 (30%), Positives = 22/49 (44%)
Frame = +2
Query: 233 VRKSGHKVKTEKINGILAAVDATKEPDLASRFGVKGYPTLKYFNKGEFK 379
+ KSG + +NGI + V A + DLA F + F K F+
Sbjct: 28 ISKSGTYPQGFSLNGIASGVKANGKKDLAILFSSRPCNAAAVFTKNAFQ 76
>SPBC3D6.05 |ptp4||phosphatidate cytidylyltransferase Ptp4
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 218
Score = 25.4 bits (53), Expect = 6.8
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = -2
Query: 333 TPNLDAKSGSFVASTAANIPLIFSVFTLWPLFRT 232
TP + AK+ S S A + +F + W LFRT
Sbjct: 133 TPKI-AKNKSLAGSLGAFVCGVFCCYVYWGLFRT 165
>SPAC16E8.14c |||methyltransferase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 219
Score = 25.0 bits (52), Expect = 9.0
Identities = 11/33 (33%), Positives = 19/33 (57%), Gaps = 1/33 (3%)
Frame = +1
Query: 493 PCATSILDFQKHTPEKSKHSIVMFYAPWC-GHL 588
PC+ + Q TPEK+++ ++ + WC HL
Sbjct: 103 PCSFINVGLQNWTPEKNRYGLI--WNQWCLSHL 133
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,421,694
Number of Sequences: 5004
Number of extensions: 43935
Number of successful extensions: 159
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 140
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 159
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 277683324
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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