Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0871
(681 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 134 8e-33
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 50 3e-07
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 44 2e-05
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 42 1e-04
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 41 2e-04
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 41 2e-04
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 38 0.002
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 38 0.002
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 38 0.002
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 36 0.007
SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 35 0.009
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 34 0.022
SPAC3F10.11c |abc2||glutathione S-conjugate-exporting ATPase Abc... 31 0.15
SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosacch... 30 0.36
SPBC359.05 |abc3||ABC transporter Abc3|Schizosaccharomyces pombe... 29 0.62
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 29 0.62
SPAC2H10.02c |||26S proteasome regulator |Schizosaccharomyces po... 29 0.82
SPAP8A3.11c |||mitochondrial GTPase Mtg2|Schizosaccharomyces pom... 27 2.5
SPBC30D10.18c |rpl102|rpl1-2, rpl10a-2|60S ribosomal protein L10... 27 2.5
SPBP8B7.22 |erd2||HDEL receptor|Schizosaccharomyces pombe|chr 2|... 25 7.7
SPMIT.06 |||mitochondrial DNA binding endonuclease|Schizosacchar... 25 7.7
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 134 bits (325), Expect = 8e-33
Identities = 60/86 (69%), Positives = 68/86 (79%)
Frame = +3
Query: 249 TLAEKQIFERTKPHCNVGTIGHVDHGKTTLTAAITKVLSDLNLAQKKGYADIDNAPEEKA 428
T A++++F R KPH N+GTIGHVDHGKTTLTAAITK LSDL A Y+ ID APEEKA
Sbjct: 40 TYADEKVFVRKKPHVNIGTIGHVDHGKTTLTAAITKCLSDLGQASFMDYSQIDKAPEEKA 99
Query: 429 RGITINVAHVEYQTEQRHYGHTDCPG 506
RGITI+ AHVEY+T RHY H DCPG
Sbjct: 100 RGITISSAHVEYETANRHYAHVDCPG 125
Score = 50.8 bits (116), Expect = 2e-07
Identities = 23/29 (79%), Positives = 25/29 (86%)
Frame = +2
Query: 500 PRSADYIKNMITGTAQMDGAILVVAATDG 586
P ADYIKNMITG A MDGAI+VV+ATDG
Sbjct: 124 PGHADYIKNMITGAATMDGAIIVVSATDG 152
Score = 36.7 bits (81), Expect = 0.003
Identities = 14/21 (66%), Positives = 19/21 (90%)
Frame = +1
Query: 589 MPQTREHLLLXQQIGIQHVVV 651
MPQTREHLLL +Q+G++ +VV
Sbjct: 154 MPQTREHLLLARQVGVKQIVV 174
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 50.0 bits (114), Expect = 3e-07
Identities = 40/133 (30%), Positives = 66/133 (49%), Gaps = 17/133 (12%)
Frame = +3
Query: 159 EISSEAKPVCKCTKHNSPVRIDSIKYNTQETLAEKQIFERT--KPHCNVGTIGHVDHGKT 332
+ + E K N P + K N + ++ +IFE++ KP ++ GHVD GK+
Sbjct: 132 QTNGEVKMKNSSESDNQPEKKKIKKQNPTDLVSVPEIFEQSNPKPVVHLVVTGHVDSGKS 191
Query: 333 TLTAAITKVLSDLN--LAQK---------KG---YA-DIDNAPEEKARGITINVAHVEYQ 467
T+ I L ++N QK KG YA +D EE+ARG+T++VA ++
Sbjct: 192 TMLGRIMFELGEINSRSMQKLHNEAANSGKGSFSYAWLLDTTEEERARGVTMDVASTTFE 251
Query: 468 TEQRHYGHTDCPG 506
++++ Y D PG
Sbjct: 252 SDKKIYEIGDAPG 264
Score = 27.1 bits (57), Expect = 2.5
Identities = 12/23 (52%), Positives = 15/23 (65%)
Frame = +1
Query: 595 QTREHLLLXQQIGIQHVVVS*TK 663
QTREH L + +GI +VVS K
Sbjct: 302 QTREHAYLLRALGISEIVVSVNK 324
Score = 25.4 bits (53), Expect = 7.7
Identities = 11/24 (45%), Positives = 14/24 (58%)
Frame = +2
Query: 500 PRSADYIKNMITGTAQMDGAILVV 571
P D+I MI G + D A+LVV
Sbjct: 263 PGHRDFISGMIAGASSADFAVLVV 286
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 44.0 bits (99), Expect = 2e-05
Identities = 29/76 (38%), Positives = 36/76 (47%), Gaps = 5/76 (6%)
Frame = +3
Query: 294 NVGTIGHVDHGKTTLTAAITKVLSDLNLAQKKGYAD-----IDNAPEEKARGITINVAHV 458
NVG I H+D GKTTLT K+L G D +D P E+ RGITIN A +
Sbjct: 30 NVGIIAHIDAGKTTLT---EKMLYYGGFTSHFGNVDTGDTVMDYLPAERQRGITINSAAI 86
Query: 459 EYQTEQRHYGHTDCPG 506
+ + D PG
Sbjct: 87 SFTWRNQRINLIDTPG 102
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 41.5 bits (93), Expect = 1e-04
Identities = 28/90 (31%), Positives = 42/90 (46%), Gaps = 15/90 (16%)
Frame = +3
Query: 282 KPHCNVGTIGHVDHGKTTLTAAIT------------KVLSDLNLAQKKGYA---DIDNAP 416
K H N+ IGHVD GK+TL I K+ + A K+ + +D+
Sbjct: 236 KEHVNIVFIGHVDAGKSTLGGNILFLTGMVDKRTMEKIEREAKEAGKESWYLSWALDSTS 295
Query: 417 EEKARGITINVAHVEYQTEQRHYGHTDCPG 506
EE+ +G T+ V ++TE R + D PG
Sbjct: 296 EEREKGKTVEVGRAYFETEHRRFSLLDAPG 325
Score = 26.2 bits (55), Expect = 4.4
Identities = 11/19 (57%), Positives = 14/19 (73%)
Frame = +1
Query: 595 QTREHLLLXQQIGIQHVVV 651
QTREH +L + GI H+VV
Sbjct: 363 QTREHAVLARTQGINHLVV 381
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 40.7 bits (91), Expect = 2e-04
Identities = 18/50 (36%), Positives = 30/50 (60%)
Frame = +3
Query: 294 NVGTIGHVDHGKTTLTAAITKVLSDLNLAQKKGYADIDNAPEEKARGITI 443
N+ I HVDHGK+TLT ++ + ++ A+ +D +E+ RG+TI
Sbjct: 21 NMSVIAHVDHGKSTLTDSLVQKAGIISAAKAGDARFMDTRADEQERGVTI 70
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 40.7 bits (91), Expect = 2e-04
Identities = 18/50 (36%), Positives = 30/50 (60%)
Frame = +3
Query: 294 NVGTIGHVDHGKTTLTAAITKVLSDLNLAQKKGYADIDNAPEEKARGITI 443
N+ I HVDHGK+TLT ++ + ++ A+ +D +E+ RG+TI
Sbjct: 21 NMSVIAHVDHGKSTLTDSLVQKAGIISAAKAGDARFMDTRADEQERGVTI 70
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 37.5 bits (83), Expect = 0.002
Identities = 28/92 (30%), Positives = 42/92 (45%), Gaps = 15/92 (16%)
Frame = +3
Query: 276 RTKPHCNVGTIGHVDHGKTTLTAAI---------------TKVLSDLNLAQKKGYADIDN 410
+ K H NV IGHVD GK+T T + K ++L K +D
Sbjct: 3 KEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVLDK 62
Query: 411 APEEKARGITINVAHVEYQTEQRHYGHTDCPG 506
E+ RGITI++A +++T + + D PG
Sbjct: 63 LKAERERGITIDIALWKFETPKYNVTVIDAPG 94
Score = 36.7 bits (81), Expect = 0.003
Identities = 16/29 (55%), Positives = 20/29 (68%)
Frame = +2
Query: 500 PRSADYIKNMITGTAQMDGAILVVAATDG 586
P D+IKNMITGT+Q D AIL++ G
Sbjct: 93 PGHRDFIKNMITGTSQADCAILIIGGGTG 121
Score = 25.8 bits (54), Expect = 5.8
Identities = 10/23 (43%), Positives = 16/23 (69%)
Frame = +1
Query: 595 QTREHLLLXQQIGIQHVVVS*TK 663
QTREH LL +G++ ++V+ K
Sbjct: 132 QTREHALLAYTLGVKQLIVAVNK 154
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 37.5 bits (83), Expect = 0.002
Identities = 28/92 (30%), Positives = 42/92 (45%), Gaps = 15/92 (16%)
Frame = +3
Query: 276 RTKPHCNVGTIGHVDHGKTTLTAAI---------------TKVLSDLNLAQKKGYADIDN 410
+ K H NV IGHVD GK+T T + K ++L K +D
Sbjct: 3 KEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVLDK 62
Query: 411 APEEKARGITINVAHVEYQTEQRHYGHTDCPG 506
E+ RGITI++A +++T + + D PG
Sbjct: 63 LKAERERGITIDIALWKFETPKYNVTVIDAPG 94
Score = 36.3 bits (80), Expect = 0.004
Identities = 15/29 (51%), Positives = 20/29 (68%)
Frame = +2
Query: 500 PRSADYIKNMITGTAQMDGAILVVAATDG 586
P D+IKNMITGT+Q D A+L++ G
Sbjct: 93 PGHRDFIKNMITGTSQADCAVLIIGGGTG 121
Score = 25.8 bits (54), Expect = 5.8
Identities = 10/23 (43%), Positives = 16/23 (69%)
Frame = +1
Query: 595 QTREHLLLXQQIGIQHVVVS*TK 663
QTREH LL +G++ ++V+ K
Sbjct: 132 QTREHALLAYTLGVKQLIVAVNK 154
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 37.5 bits (83), Expect = 0.002
Identities = 28/92 (30%), Positives = 42/92 (45%), Gaps = 15/92 (16%)
Frame = +3
Query: 276 RTKPHCNVGTIGHVDHGKTTLTAAI---------------TKVLSDLNLAQKKGYADIDN 410
+ K H NV IGHVD GK+T T + K ++L K +D
Sbjct: 3 KEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVLDK 62
Query: 411 APEEKARGITINVAHVEYQTEQRHYGHTDCPG 506
E+ RGITI++A +++T + + D PG
Sbjct: 63 LKAERERGITIDIALWKFETPKYNVTVIDAPG 94
Score = 36.7 bits (81), Expect = 0.003
Identities = 16/29 (55%), Positives = 20/29 (68%)
Frame = +2
Query: 500 PRSADYIKNMITGTAQMDGAILVVAATDG 586
P D+IKNMITGT+Q D AIL++ G
Sbjct: 93 PGHRDFIKNMITGTSQADCAILIIGGGTG 121
Score = 25.8 bits (54), Expect = 5.8
Identities = 10/23 (43%), Positives = 16/23 (69%)
Frame = +1
Query: 595 QTREHLLLXQQIGIQHVVVS*TK 663
QTREH LL +G++ ++V+ K
Sbjct: 132 QTREHALLAYTLGVKQLIVAVNK 154
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 35.5 bits (78), Expect = 0.007
Identities = 18/50 (36%), Positives = 27/50 (54%)
Frame = +3
Query: 294 NVGTIGHVDHGKTTLTAAITKVLSDLNLAQKKGYADIDNAPEEKARGITI 443
N I H+DHGK+TL+ I K+ +N + +D E+ RGIT+
Sbjct: 60 NWAVIAHIDHGKSTLSDCILKLTGVINEHNFRNQF-LDKLEVERRRGITV 108
>SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 446
Score = 35.1 bits (77), Expect = 0.009
Identities = 19/43 (44%), Positives = 27/43 (62%), Gaps = 7/43 (16%)
Frame = +3
Query: 294 NVGTIGHVDHGKTTLTAAITKVLS-------DLNLAQKKGYAD 401
N+GTIGHV HGK+T+ AI+ V + + N+ K GYA+
Sbjct: 25 NIGTIGHVAHGKSTVVKAISGVHTVRFKNELERNITIKLGYAN 67
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 33.9 bits (74), Expect = 0.022
Identities = 26/77 (33%), Positives = 38/77 (49%), Gaps = 5/77 (6%)
Frame = +3
Query: 237 NTQETLAEKQIFERTKPHCNVGTIGHVDHGKTTLTAAI---TKVLSDLNLAQKKGY--AD 401
N QE L + +R K N+G H+D GKTT T + T + D++ + K A
Sbjct: 43 NIQEQLNDNDK-KRLKQIRNIGISAHIDSGKTTFTERVLYYTGRIKDIHEVRGKDNVGAK 101
Query: 402 IDNAPEEKARGITINVA 452
+D E+ +GITI A
Sbjct: 102 MDFMELEREKGITIQSA 118
>SPAC3F10.11c |abc2||glutathione S-conjugate-exporting ATPase
Abc2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1463
Score = 31.1 bits (67), Expect = 0.15
Identities = 14/46 (30%), Positives = 23/46 (50%)
Frame = +3
Query: 225 SIKYNTQETLAEKQIFERTKPHCNVGTIGHVDHGKTTLTAAITKVL 362
S++Y L I KP +G +G GK+TLT A+ +++
Sbjct: 1230 SVRYRENLPLVLNDISVNIKPQEKIGIVGRTGAGKSTLTLALFRLI 1275
>SPBC1271.15c |||translation initiation factor
IF-2Mt|Schizosaccharomyces pombe|chr 2|||Manual
Length = 686
Score = 29.9 bits (64), Expect = 0.36
Identities = 13/20 (65%), Positives = 14/20 (70%)
Frame = +3
Query: 297 VGTIGHVDHGKTTLTAAITK 356
V +GHVDHGKTTL A K
Sbjct: 174 VTLMGHVDHGKTTLLDAFRK 193
>SPBC359.05 |abc3||ABC transporter Abc3|Schizosaccharomyces pombe|chr
2|||Manual
Length = 1465
Score = 29.1 bits (62), Expect = 0.62
Identities = 22/85 (25%), Positives = 35/85 (41%), Gaps = 1/85 (1%)
Frame = +3
Query: 159 EISSEAKPVCKCTKHNS-PVRIDSIKYNTQETLAEKQIFERTKPHCNVGTIGHVDHGKTT 335
EI E +P C+ + S KY + A I P +G +G GK+T
Sbjct: 1209 EIIPENRPPCEWPTDGAVSFNHYSAKYREDLSFALNNINIEISPREKIGIVGRTGAGKST 1268
Query: 336 LTAAITKVLSDLNLAQKKGYADIDN 410
L A+ +++ +G +IDN
Sbjct: 1269 LAMALFRIIE-----PTEGKIEIDN 1288
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1000
Score = 29.1 bits (62), Expect = 0.62
Identities = 16/50 (32%), Positives = 24/50 (48%)
Frame = +3
Query: 294 NVGTIGHVDHGKTTLTAAITKVLSDLNLAQKKGYADIDNAPEEKARGITI 443
N + HVDHGKTTL ++ ++ +D +E RGIT+
Sbjct: 21 NFTLLAHVDHGKTTLADSLLASNGIISSKLAGTVRFLDFREDEITRGITM 70
Score = 25.4 bits (53), Expect = 7.7
Identities = 10/31 (32%), Positives = 17/31 (54%)
Frame = +2
Query: 500 PRSADYIKNMITGTAQMDGAILVVAATDGVC 592
P D+ + + + DGA ++V A +GVC
Sbjct: 102 PGHVDFSSEVSSASRLCDGAFVLVDAVEGVC 132
>SPAC2H10.02c |||26S proteasome regulator |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 213
Score = 28.7 bits (61), Expect = 0.82
Identities = 12/48 (25%), Positives = 22/48 (45%)
Frame = +3
Query: 165 SSEAKPVCKCTKHNSPVRIDSIKYNTQETLAEKQIFERTKPHCNVGTI 308
S A K + P+ ++ YN + ++ +I R KP C V ++
Sbjct: 88 SLAANDETKLAQEADPLNFNAANYNMNDIISRSKILGRVKPFCVVDSV 135
>SPAP8A3.11c |||mitochondrial GTPase Mtg2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 419
Score = 27.1 bits (57), Expect = 2.5
Identities = 14/44 (31%), Positives = 24/44 (54%), Gaps = 1/44 (2%)
Frame = +3
Query: 225 SIKYNTQETLAEKQIFE-RTKPHCNVGTIGHVDHGKTTLTAAIT 353
S K+ T+ E+++ E K C +G +G + GK+TL +T
Sbjct: 214 SPKFATKGLTGEQKLIELELKTICEIGLVGLPNAGKSTLLNCLT 257
>SPBC30D10.18c |rpl102|rpl1-2, rpl10a-2|60S ribosomal protein
L10a|Schizosaccharomyces pombe|chr 2|||Manual
Length = 216
Score = 27.1 bits (57), Expect = 2.5
Identities = 20/72 (27%), Positives = 30/72 (41%)
Frame = +3
Query: 189 KCTKHNSPVRIDSIKYNTQETLAEKQIFERTKPHCNVGTIGHVDHGKTTLTAAITKVLSD 368
K K SPV Y + F+ K C +GHVD + L A ++ ++
Sbjct: 129 KAGKFPSPVSHSDDLYGKIIEVKSTIKFQLKKVLCLGVAVGHVDMAEEQLAANLSLAINF 188
Query: 369 LNLAQKKGYADI 404
L KKG+ +I
Sbjct: 189 LVSLLKKGWQNI 200
>SPBP8B7.22 |erd2||HDEL receptor|Schizosaccharomyces pombe|chr
2|||Manual
Length = 212
Score = 25.4 bits (53), Expect = 7.7
Identities = 11/37 (29%), Positives = 24/37 (64%)
Frame = -2
Query: 116 MLNELFNTKRC*ILNFQKFRLIFVWIFYNKYIHLFFR 6
+L+ + +K C L+ K L+F+ ++ +Y++LF+R
Sbjct: 19 LLHRMKKSKTCSGLSL-KSHLLFLLVYVTRYLNLFWR 54
>SPMIT.06 |||mitochondrial DNA binding
endonuclease|Schizosaccharomyces pombe|chr
mitochondrial|||Manual
Length = 807
Score = 25.4 bits (53), Expect = 7.7
Identities = 14/51 (27%), Positives = 23/51 (45%)
Frame = +3
Query: 336 LTAAITKVLSDLNLAQKKGYADIDNAPEEKARGITINVAHVEYQTEQRHYG 488
+ A IT S + L I N+ +K + N++H + T RH+G
Sbjct: 529 ILAKITCFCSSIGLTVSPTKTKITNSYTDKILFLGTNISHSKNVTFSRHFG 579
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,671,904
Number of Sequences: 5004
Number of extensions: 50642
Number of successful extensions: 156
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 129
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 155
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 313902888
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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