Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= e96h0862
         (816 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC222.12c |atp2||F1-ATPase beta subunit |Schizosaccharomyces p...   144   1e-35
SPAC14C4.14 |atp1||F1-ATPase alpha subunit|Schizosaccharomyces p...    59   9e-10
SPAC637.05c |vma2||V-type ATPase V1 subunit B |Schizosaccharomyc...    41   2e-04
SPAC343.05 |vma1||V-type ATPase subunit A|Schizosaccharomyces po...    33   0.037
SPAC23C11.10 |||conserved eukaryotic protein|Schizosaccharomyces...    28   1.8  
SPBC26H8.01 |thi2|nmt2|thiazole biosynthetic enzyme|Schizosaccha...    27   2.4  
SPBC1826.01c |mot1||TATA-binding protein associated factor Mot1|...    27   2.4  
SPBC1A4.06c |||mitochondrial matrix protein import protein|Schiz...    27   2.4  
SPBC11G11.04 |trs20||TRAPP complex subunit Trs20 |Schizosaccharo...    27   2.4  
SPAC227.16c |||GINS complex subunit Psf3|Schizosaccharomyces pom...    27   3.2  
SPAC13G7.04c |mac1||membrane anchored protein Mac1 |Schizosaccha...    27   4.2  
SPAC17G6.16c |ysh1||mRNA cleavage and polyadenylation specificit...    26   5.6  
SPAC17C9.06 |sam50||SAM complex subunit Sam50 |Schizosaccharomyc...    25   9.7  
SPAC2G11.10c |||URM1 activating enzyme |Schizosaccharomyces pomb...    25   9.7  

>SPAC222.12c |atp2||F1-ATPase beta subunit |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 525

 Score =  144 bits (349), Expect = 1e-35
 Identities = 70/85 (82%), Positives = 76/85 (89%)
 Frame = +3

Query: 255 SGVGKTVLIMELINNVAKAHGGYSVFAGVGERTREGNDLYHEMIESGVISLKDKTSKVAL 434
           +GVGKTV I ELINN+AKAHGGYSVF GVGERTREGNDLY EM E+GVI L+ + SK AL
Sbjct: 205 AGVGKTVFIQELINNIAKAHGGYSVFTGVGERTREGNDLYREMQETGVIKLEGE-SKAAL 263

Query: 435 VYGQMNEPPGARARVALTGLTVAEY 509
           V+GQMNEPPGARARVALTGLTVAEY
Sbjct: 264 VFGQMNEPPGARARVALTGLTVAEY 288



 Score =  142 bits (343), Expect = 7e-35
 Identities = 74/102 (72%), Positives = 77/102 (75%)
 Frame = +2

Query: 509 FRDQEGQDVLLFIDNIFRFTQAGSEVSALLGRIPSAVGYQPTLATDMGTMQERITTTKKG 688
           FRD EGQDVLLFIDNIFRFTQAGSEVSALLGRIPSAVGYQPTLATDMG MQERITTTKKG
Sbjct: 289 FRDIEGQDVLLFIDNIFRFTQAGSEVSALLGRIPSAVGYQPTLATDMGAMQERITTTKKG 348

Query: 689 SITSVQAIYVXXXXXXXXXXXXXXXXXXXXXYSSRAIAELGI 814
           SITSVQA+YV                       SR+I+ELGI
Sbjct: 349 SITSVQAVYVPADDLTDPAPATTFAHLDATTVLSRSISELGI 390



 Score =  124 bits (300), Expect = 1e-29
 Identities = 59/82 (71%), Positives = 67/82 (81%)
 Frame = +2

Query: 8   VLDSGSPIRIPVGAETLGRIINVIGEPIDERGPIPTDKTAAIHAEAPEFVDMSVQXEILV 187
           V+D+GSPI IPVG  TLGRI+NVIGEP+DERGPI   K + IHA+AP F + S   EIL 
Sbjct: 122 VIDTGSPISIPVGPGTLGRIMNVIGEPVDERGPIKAVKYSPIHADAPSFEEQSTTPEILE 181

Query: 188 TGIKVVDLLAPYAKGGKIGLFG 253
           TGIKVVDLLAPYA+GGKIGLFG
Sbjct: 182 TGIKVVDLLAPYARGGKIGLFG 203


>SPAC14C4.14 |atp1||F1-ATPase alpha subunit|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 536

 Score = 58.8 bits (136), Expect = 9e-10
 Identities = 24/76 (31%), Positives = 44/76 (57%)
 Frame = +2

Query: 29  IRIPVGAETLGRIINVIGEPIDERGPIPTDKTAAIHAEAPEFVDMSVQXEILVTGIKVVD 208
           + +PVG   LGR+++ +G PID +GPI T +   +  +AP  +  +   E + TG+K +D
Sbjct: 123 VDVPVGEALLGRVVDALGNPIDGKGPIKTTERRRVQLKAPGILPRTSVCEPMQTGLKAID 182

Query: 209 LLAPYAKGGKIGLFGE 256
            + P  +G +  + G+
Sbjct: 183 SMVPIGRGQRELIIGD 198


>SPAC637.05c |vma2||V-type ATPase V1 subunit B |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 503

 Score = 41.1 bits (92), Expect = 2e-04
 Identities = 25/78 (32%), Positives = 38/78 (48%)
 Frame = +2

Query: 17  SGSPIRIPVGAETLGRIINVIGEPIDERGPIPTDKTAAIHAEAPEFVDMSVQXEILVTGI 196
           +G  +RIPV  + LGR+ N  G PID+   +  +    I+             E++ TGI
Sbjct: 92  TGHSMRIPVSEDMLGRVFNGSGLPIDKGPNLLAEDYLDINGSPINPYARIYPEEMIQTGI 151

Query: 197 KVVDLLAPYAKGGKIGLF 250
             +D L   A+G KI +F
Sbjct: 152 SSIDGLNSIARGQKIPIF 169



 Score = 33.1 bits (72), Expect = 0.048
 Identities = 20/64 (31%), Positives = 31/64 (48%), Gaps = 2/64 (3%)
 Frame = +2

Query: 518 QEGQDVLLFIDNIFRFTQAGSEVSALLGRIPSAVGYQPTLATDMGTMQERI--TTTKKGS 691
           Q  + VL  + ++  +  A  EVSA    +P   GY   + TD+ T+ ER      + GS
Sbjct: 266 QTEKHVLTILTDMTSYADALREVSAAREEVPGRRGYPGYMYTDLSTIYERAGRVEGRNGS 325

Query: 692 ITSV 703
           IT +
Sbjct: 326 ITQI 329


>SPAC343.05 |vma1||V-type ATPase subunit A|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 619

 Score = 33.5 bits (73), Expect = 0.037
 Identities = 28/89 (31%), Positives = 41/89 (46%), Gaps = 3/89 (3%)
 Frame = +3

Query: 252 ASGVGKTVLIMELINNVAKAHGGYSVFAGVGERTREGNDLYHEMIESGV-ISLKDKT--S 422
           A G GKTV+   L      ++    V+ G GER  E  ++  +  E  + I+ K +    
Sbjct: 259 AFGCGKTVISQSLSKY---SNSDLIVYVGCGERGNEMAEVLMDFPELTIDINGKPEPIMK 315

Query: 423 KVALVYGQMNEPPGARARVALTGLTVAEY 509
           +  LV    N P  AR     TG+T+AEY
Sbjct: 316 RTTLVANTSNMPVAAREASIYTGITLAEY 344



 Score = 33.5 bits (73), Expect = 0.037
 Identities = 17/53 (32%), Positives = 29/53 (54%)
 Frame = +2

Query: 509 FRDQEGQDVLLFIDNIFRFTQAGSEVSALLGRIPSAVGYQPTLATDMGTMQER 667
           +RDQ G++V +  D+  R+ +A  E+S  L  +P+  GY   L   + +  ER
Sbjct: 345 YRDQ-GKNVSMMADSTSRWAEALREISGRLAEMPADSGYPAYLGAKLASFYER 396



 Score = 26.2 bits (55), Expect = 5.6
 Identities = 13/33 (39%), Positives = 20/33 (60%), Gaps = 1/33 (3%)
 Frame = +2

Query: 182 LVTGIKVVDLLAPYAKGGKIGLFGEW-CGQNCI 277
           L+TG +V+D L P  +GG   + G + CG+  I
Sbjct: 235 LLTGQRVLDALYPCVQGGTTAIPGAFGCGKTVI 267


>SPAC23C11.10 |||conserved eukaryotic protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 265

 Score = 27.9 bits (59), Expect = 1.8
 Identities = 13/50 (26%), Positives = 26/50 (52%), Gaps = 1/50 (2%)
 Frame = -3

Query: 661 LHSTHVSSQSRLISYSRWDTTKQSRHF*SSLSETENV-VNEEQYILSFLI 515
           LH +  SS +    Y  W +  +  HF  +++E  N  + ++Q+  SF++
Sbjct: 198 LHISFASSLTNEDEYQNWVSQDRESHFFKTMNEIINTKIQKDQFSESFIV 247


>SPBC26H8.01 |thi2|nmt2|thiazole biosynthetic
           enzyme|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 328

 Score = 27.5 bits (58), Expect = 2.4
 Identities = 19/82 (23%), Positives = 29/82 (35%)
 Frame = +2

Query: 68  INVIGEPIDERGPIPTDKTAAIHAEAPEFVDMSVQXEILVTGIKVVDLLAPYAKGGKIGL 247
           +N IG P ++ G     K AA+         +++    L     V DL+    K GK  +
Sbjct: 137 LNEIGVPYEDEGDYVVVKHAALFTSTVMARTLALPNVKLFNATAVEDLIVKEGKDGKQRI 196

Query: 248 FGEWCGQNCIDYGTDQQCCQSP 313
            G       +      Q C  P
Sbjct: 197 AGVVTNWTLVSLNHGLQSCMDP 218


>SPBC1826.01c |mot1||TATA-binding protein associated factor
            Mot1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1953

 Score = 27.5 bits (58), Expect = 2.4
 Identities = 7/23 (30%), Positives = 18/23 (78%)
 Frame = -1

Query: 444  DHILELPWMFCLLEKSHQIQSSR 376
            D ++++ W +C+L++ H I+++R
Sbjct: 1481 DELVKIDWNYCVLDEGHVIKNAR 1503


>SPBC1A4.06c |||mitochondrial matrix protein import
           protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 383

 Score = 27.5 bits (58), Expect = 2.4
 Identities = 22/64 (34%), Positives = 35/64 (54%), Gaps = 4/64 (6%)
 Frame = -3

Query: 292 ISSIINTVLPTPLAK----QPNLSSFGIRSEQIDDFYTSYENLLLHRHVDKLWSFSMNSS 125
           IS ++N  LP  L K    +P+ SSF   +E +     S  +LL+ + + KL SFS+ + 
Sbjct: 304 ISRLLNG-LPLNLVKILGLKPDTSSFEKCAELMLTNQISTRSLLISKSIKKLTSFSILTQ 362

Query: 124 SLVG 113
           S+ G
Sbjct: 363 SIKG 366


>SPBC11G11.04 |trs20||TRAPP complex subunit Trs20
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 136

 Score = 27.5 bits (58), Expect = 2.4
 Identities = 13/37 (35%), Positives = 19/37 (51%), Gaps = 1/37 (2%)
 Frame = -2

Query: 296 VDQFHNQYSFAHTTRQTTQSFLLWHKERADR-RLLYQ 189
           +DQFH  Y  A+ T    +  LL   + AD  +L +Q
Sbjct: 62  IDQFHEMYISAYVTPSNMRFMLLHQNQSADNIKLFFQ 98


>SPAC227.16c |||GINS complex subunit Psf3|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 166

 Score = 27.1 bits (57), Expect = 3.2
 Identities = 23/79 (29%), Positives = 34/79 (43%), Gaps = 7/79 (8%)
 Frame = -3

Query: 343 PTPANTE*PPWALATLLISSIINTVLPTPL------AKQPNLSSFGIRSEQIDDFYTSYE 182
           PT +  E P W    L I+S ++  +P P       A + N +S  IR   I   Y  + 
Sbjct: 35  PTGSKVELPFWLAEVLAINSFVSIHMPAPFSSVVRNALKANPNSVSIR--DITTHYYHFA 92

Query: 181 NLLLHR-HVDKLWSFSMNS 128
             +LH    D L   S+N+
Sbjct: 93  EKMLHLISDDSLVQISLNT 111


>SPAC13G7.04c |mac1||membrane anchored protein Mac1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 756

 Score = 26.6 bits (56), Expect = 4.2
 Identities = 11/24 (45%), Positives = 14/24 (58%)
 Frame = -1

Query: 270 FCPHHSPNNPIFPPLA*GASRSTT 199
           +CPH + N P FP L    +R TT
Sbjct: 310 YCPHKTGNRPGFPSLNIPRTRPTT 333


>SPAC17G6.16c |ysh1||mRNA cleavage and polyadenylation specificity
           factor complex subunit Ysh1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 775

 Score = 26.2 bits (55), Expect = 5.6
 Identities = 12/32 (37%), Positives = 19/32 (59%)
 Frame = -3

Query: 295 LISSIINTVLPTPLAKQPNLSSFGIRSEQIDD 200
           ++S  INT+LP   A Q ++S     +E+ DD
Sbjct: 701 VLSRAINTILPFSEASQNDVSEDDFENEESDD 732


>SPAC17C9.06 |sam50||SAM complex subunit Sam50 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 475

 Score = 25.4 bits (53), Expect = 9.7
 Identities = 11/26 (42%), Positives = 14/26 (53%)
 Frame = -2

Query: 536 VHPVLLDHGIFSNSETSKGNTSTGTR 459
           VH  +L   +F  +E   GN S GTR
Sbjct: 148 VHANVLARNVFGGAELLSGNVSYGTR 173


>SPAC2G11.10c |||URM1 activating enzyme |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 401

 Score = 25.4 bits (53), Expect = 9.7
 Identities = 9/21 (42%), Positives = 12/21 (57%)
 Frame = +3

Query: 735 QICSSYHFCYLGCYHCTLPEP 797
           Q+C  Y++C   CY C  P P
Sbjct: 172 QLCI-YNYCNGPCYRCMFPNP 191


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,317,473
Number of Sequences: 5004
Number of extensions: 67814
Number of successful extensions: 223
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 212
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 220
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 398435810
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)