Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0851
(739 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 155 5e-39
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 152 4e-38
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 97 2e-21
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 69 5e-13
SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr... 28 1.6
SPAC16A10.01 |||DUF1212 family protein|Schizosaccharomyces pombe... 27 2.8
SPBC354.07c |||oxysterol binding protein |Schizosaccharomyces po... 27 2.8
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 6.4
SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom... 25 8.5
SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyce... 25 8.5
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 155 bits (377), Expect = 5e-39
Identities = 66/95 (69%), Positives = 80/95 (84%)
Frame = +2
Query: 224 FQHFLQRDRSCKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHY 403
F F K+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHY
Sbjct: 49 FSTFFSETGQGKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHY 108
Query: 404 TIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGG 508
T+GKE+VD V D+IR++AD C+GLQGFL+FHSFGG
Sbjct: 109 TVGKELVDEVTDKIRRIADNCSGLQGFLVFHSFGG 143
Score = 117 bits (282), Expect = 2e-27
Identities = 50/69 (72%), Positives = 63/69 (91%)
Frame = +1
Query: 511 TGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMV 690
TGSGF +LL+ERL+++Y KKSKL+F++YPAPQVST+VVEPYNS+LTTH TL+ +DC FMV
Sbjct: 145 TGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMV 204
Query: 691 DNEAIYDIC 717
DNE+ YDIC
Sbjct: 205 DNESCYDIC 213
Score = 94.3 bits (224), Expect = 2e-20
Identities = 41/63 (65%), Positives = 45/63 (71%)
Frame = +3
Query: 81 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAAS 260
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 261 TYP 269
P
Sbjct: 61 YVP 63
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 152 bits (369), Expect = 4e-38
Identities = 63/95 (66%), Positives = 80/95 (84%)
Frame = +2
Query: 224 FQHFLQRDRSCKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHY 403
F F K VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHY
Sbjct: 53 FGTFFSETGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHY 112
Query: 404 TIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGG 508
T+GKE++D VL+RIR++AD C+GLQGFL+FHSFGG
Sbjct: 113 TVGKEMIDSVLERIRRMADNCSGLQGFLVFHSFGG 147
Score = 120 bits (289), Expect = 2e-28
Identities = 51/69 (73%), Positives = 63/69 (91%)
Frame = +1
Query: 511 TGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMV 690
TGSG +LL+ERL+++YGKKS L+F++YPAPQVST+VVEPYNS+LTTH TL++SDC FMV
Sbjct: 149 TGSGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMV 208
Query: 691 DNEAIYDIC 717
DNEA YDIC
Sbjct: 209 DNEACYDIC 217
Score = 88.2 bits (209), Expect = 1e-18
Identities = 45/68 (66%), Positives = 47/68 (69%), Gaps = 5/68 (7%)
Frame = +3
Query: 81 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 245
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 246 TGAASTYP 269
TG P
Sbjct: 60 TGQGKFVP 67
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 97.5 bits (232), Expect = 2e-21
Identities = 43/84 (51%), Positives = 58/84 (69%)
Frame = +2
Query: 257 KHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVL 436
K+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VL
Sbjct: 58 KYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVL 117
Query: 437 DRIRKLADQCTGLQGFLIFHSFGG 508
D +R+ A+ C LQGF + HS GG
Sbjct: 118 DVVRREAEACDALQGFQLTHSLGG 141
Score = 66.1 bits (154), Expect = 5e-12
Identities = 30/70 (42%), Positives = 45/70 (64%)
Frame = +1
Query: 511 TGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMV 690
TGSG +LL+ ++ +Y + F++ PAP+ S VVEPYN+ L+ H +E+SD F +
Sbjct: 143 TGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCI 202
Query: 691 DNEAIYDICA 720
DNEA+ I A
Sbjct: 203 DNEALSSIFA 212
Score = 36.3 bits (80), Expect = 0.005
Identities = 19/63 (30%), Positives = 26/63 (41%)
Frame = +3
Query: 81 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAAS 260
MRE + + GQ G Q+G A W EHG+ G T + N +F+E
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 261 TYP 269
P
Sbjct: 59 YVP 61
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 69.3 bits (162), Expect = 5e-13
Identities = 35/94 (37%), Positives = 57/94 (60%), Gaps = 2/94 (2%)
Frame = +2
Query: 233 FLQRDRSCKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYT 406
F Q D + +++PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+
Sbjct: 52 FYQSDDT-RYIPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YS 109
Query: 407 IGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGG 508
+ I + ++D I + AD L+GF + HS G
Sbjct: 110 HAERIFEDIMDMIDREADGSDSLEGFSLLHSIAG 143
Score = 53.6 bits (123), Expect = 3e-08
Identities = 29/71 (40%), Positives = 42/71 (59%), Gaps = 1/71 (1%)
Frame = +1
Query: 511 TGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQ-VSTAVVEPYNSILTTHTTLEHSDCAFM 687
TGSG S L+ERL+ Y KK ++++P Q VS VV+PYNS+L ++D +
Sbjct: 145 TGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVV 204
Query: 688 VDNEAIYDICA 720
+DN A+ I A
Sbjct: 205 LDNAALAHIAA 215
Score = 48.8 bits (111), Expect = 8e-07
Identities = 24/55 (43%), Positives = 33/55 (60%)
Frame = +3
Query: 84 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSET 248
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQS 55
>SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1024
Score = 27.9 bits (59), Expect = 1.6
Identities = 16/37 (43%), Positives = 20/37 (54%), Gaps = 2/37 (5%)
Frame = -1
Query: 316 ADLINNSRFKIDEDS--TGYVLAAPVSLKKVLKESSP 212
AD+IN SR+ ID S T + P+SL KE P
Sbjct: 455 ADIINGSRYNIDRVSPNTAFSTNIPLSLPFANKEPQP 491
>SPAC16A10.01 |||DUF1212 family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 830
Score = 27.1 bits (57), Expect = 2.8
Identities = 14/46 (30%), Positives = 25/46 (54%)
Frame = -1
Query: 568 SCRSQRRDAPSIRK*TQSRYSTEGVEDQESLQTGTLVSELADSVQN 431
S + SIR ++SR S+ G + Q S+Q+GT ++D ++
Sbjct: 98 SSQKSSNSRKSIRSQSRSRSSSVGSDSQASIQSGTNSEVVSDDEED 143
>SPBC354.07c |||oxysterol binding protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 399
Score = 27.1 bits (57), Expect = 2.8
Identities = 14/58 (24%), Positives = 25/58 (43%), Gaps = 2/58 (3%)
Frame = -1
Query: 349 FWMKQLSVCASADLINNSRF--KIDEDSTGYVLAAPVSLKKVLKESSPPPMVLSVGIW 182
F + + S +++N+ + KID GY S K + E + P + G+W
Sbjct: 205 FMAPYIELYGSTYIVSNTNYITKIDYSGRGYFRGTKNSFKATIFEKNEDPDYIVEGVW 262
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 6.4
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +2
Query: 179 WPDAHRQDHRGWRRFFQHFLQR 244
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
>SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 534
Score = 25.4 bits (53), Expect = 8.5
Identities = 15/41 (36%), Positives = 21/41 (51%)
Frame = +1
Query: 70 LKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCP 192
L+S +L+ P SR++ P S + STASSL P
Sbjct: 170 LRSSMPLVMANSLSPPSSRALKPIHSLSNPSTASSLEPSSP 210
>SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1067
Score = 25.4 bits (53), Expect = 8.5
Identities = 12/37 (32%), Positives = 22/37 (59%)
Frame = -1
Query: 283 DEDSTGYVLAAPVSLKKVLKESSPPPMVLSVGIWPSG 173
++D T Y L P+ + L +++PP +V+ +G P G
Sbjct: 834 EKDLTKY-LGPPIYTSQRLYDTTPPGVVMGLGWTPMG 869
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,053,769
Number of Sequences: 5004
Number of extensions: 64975
Number of successful extensions: 195
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 179
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 192
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 349251756
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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