Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0839
(664 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g05090.2 68416.m00553 transducin family protein / WD-40 repea... 38 0.008
At3g05090.1 68416.m00552 transducin family protein / WD-40 repea... 38 0.008
At2g04350.2 68415.m00434 long-chain-fatty-acid--CoA ligase famil... 29 2.1
At2g04350.1 68415.m00433 long-chain-fatty-acid--CoA ligase famil... 29 2.1
At3g48520.1 68416.m05296 cytochrome P450 family protein similar ... 28 6.4
At1g60570.1 68414.m06819 kelch repeat-containing F-box family pr... 27 8.4
>At3g05090.2 68416.m00553 transducin family protein / WD-40 repeat
family protein contains seven G-protein beta WD-40
repeats; similar to uncharacterized KIAA1449 protein
(gi:7959157) [Homo sapiens]
Length = 753
Score = 37.5 bits (83), Expect = 0.008
Identities = 35/123 (28%), Positives = 51/123 (41%), Gaps = 4/123 (3%)
Frame = +2
Query: 188 EKNHGAPNTGGRAPLG*--TXASANFVSIQDKTIHKYVPTTEKHTKTSLDGRVGFILPVE 361
+ N + + G P+ T S+N +S+Q H Y PT K K S+ + L +
Sbjct: 168 DANEDSSSNGANGPVTSLRTVGSSNNISVQSSPSHGYTPTIAKGHKESV-----YALAMN 222
Query: 362 GTTDQFVVGVERKFLFIQWDGEDGSKVAVLKELGEVDKDRPNNRINDGKQILVGG--CLL 535
T V G K L + WD GSK L+ G D R + G+ L G ++
Sbjct: 223 DTGTMLVSGGTEKVLRV-WDPRTGSKSMKLR--GHTDNVRVLLLDSTGRFCLSGSSDSMI 279
Query: 536 ELW 544
LW
Sbjct: 280 RLW 282
>At3g05090.1 68416.m00552 transducin family protein / WD-40 repeat
family protein contains seven G-protein beta WD-40
repeats; similar to uncharacterized KIAA1449 protein
(gi:7959157) [Homo sapiens]
Length = 753
Score = 37.5 bits (83), Expect = 0.008
Identities = 35/123 (28%), Positives = 51/123 (41%), Gaps = 4/123 (3%)
Frame = +2
Query: 188 EKNHGAPNTGGRAPLG*--TXASANFVSIQDKTIHKYVPTTEKHTKTSLDGRVGFILPVE 361
+ N + + G P+ T S+N +S+Q H Y PT K K S+ + L +
Sbjct: 168 DANEDSSSNGANGPVTSLRTVGSSNNISVQSSPSHGYTPTIAKGHKESV-----YALAMN 222
Query: 362 GTTDQFVVGVERKFLFIQWDGEDGSKVAVLKELGEVDKDRPNNRINDGKQILVGG--CLL 535
T V G K L + WD GSK L+ G D R + G+ L G ++
Sbjct: 223 DTGTMLVSGGTEKVLRV-WDPRTGSKSMKLR--GHTDNVRVLLLDSTGRFCLSGSSDSMI 279
Query: 536 ELW 544
LW
Sbjct: 280 RLW 282
>At2g04350.2 68415.m00434 long-chain-fatty-acid--CoA ligase family
protein / long-chain acyl-CoA synthetase family protein
(LACS8) similar to LACS 4 [SP|O35547] from Rattus
norvegicus, LACS 4 [SP|O60488] from Homo sapiens;
contains Pfam HMM hit: AMP-binding enzymes PF00501
Length = 720
Score = 29.5 bits (63), Expect = 2.1
Identities = 18/62 (29%), Positives = 30/62 (48%)
Frame = +2
Query: 449 LKELGEVDKDRPNNRINDGKQILVGGCLLELWVMKILQVTLRETKPLFTNWVQLKMGTRE 628
+++ G D P+ + G ++GG ++ L V +L V L TK V +K+G E
Sbjct: 1 MEDSGVNPMDSPSKGSDFGVYGIIGGGIVALLVPVLLSVVLNGTKKGKKRGVPIKVGGEE 60
Query: 629 DY 634
Y
Sbjct: 61 GY 62
>At2g04350.1 68415.m00433 long-chain-fatty-acid--CoA ligase family
protein / long-chain acyl-CoA synthetase family protein
(LACS8) similar to LACS 4 [SP|O35547] from Rattus
norvegicus, LACS 4 [SP|O60488] from Homo sapiens;
contains Pfam HMM hit: AMP-binding enzymes PF00501
Length = 720
Score = 29.5 bits (63), Expect = 2.1
Identities = 18/62 (29%), Positives = 30/62 (48%)
Frame = +2
Query: 449 LKELGEVDKDRPNNRINDGKQILVGGCLLELWVMKILQVTLRETKPLFTNWVQLKMGTRE 628
+++ G D P+ + G ++GG ++ L V +L V L TK V +K+G E
Sbjct: 1 MEDSGVNPMDSPSKGSDFGVYGIIGGGIVALLVPVLLSVVLNGTKKGKKRGVPIKVGGEE 60
Query: 629 DY 634
Y
Sbjct: 61 GY 62
>At3g48520.1 68416.m05296 cytochrome P450 family protein similar to
Cytochrome P450 94A1 (P450-dependent fatty acid
omega-hydroxylase) (SP:O81117) {Vicia sativa}; contains
Pfam profile: PF00067 cytochrome P450
Length = 506
Score = 27.9 bits (59), Expect = 6.4
Identities = 13/48 (27%), Positives = 25/48 (52%), Gaps = 3/48 (6%)
Frame = -1
Query: 295 YIFVYSFILDAHKVSACXRSSQWGP-SPSVRGSVIFFNSHRHRI--WF 161
++ F L + ++ +GP S + GS++ FN +RHR+ W+
Sbjct: 12 FLITIIFFLSSSSTKKVQENTTYGPPSYPLIGSILSFNKNRHRLLQWY 59
>At1g60570.1 68414.m06819 kelch repeat-containing F-box family
protein low similarity to SKP1 interacting partner 6
[Arabidopsis thaliana] GI:10716957; contains Pfam
profiles PF01344: Kelch motif, PF00646: F-box domain
Length = 381
Score = 27.5 bits (58), Expect = 8.4
Identities = 9/22 (40%), Positives = 16/22 (72%)
Frame = +2
Query: 464 EVDKDRPNNRINDGKQILVGGC 529
++++DRP+ + DGK + GGC
Sbjct: 156 QMERDRPSANLIDGKIYVTGGC 177
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,969,148
Number of Sequences: 28952
Number of extensions: 304327
Number of successful extensions: 745
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 734
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 745
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1393347168
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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