Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0827
(357 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_UPI00015B63B2 Cluster: PREDICTED: similar to organic an... 40 0.013
UniRef50_Q4T1P0 Cluster: Chromosome undetermined SCAF10522, whol... 34 0.63
UniRef50_Q4QEE2 Cluster: Putative uncharacterized protein; n=1; ... 33 1.5
UniRef50_A7S9N2 Cluster: Predicted protein; n=1; Nematostella ve... 33 1.5
UniRef50_Q4P4F6 Cluster: Putative uncharacterized protein; n=1; ... 33 1.5
UniRef50_A2Q2J0 Cluster: RNA-directed DNA polymerase (Reverse tr... 32 3.4
UniRef50_Q0AD47 Cluster: Twin-arginine translocation pathway sig... 31 4.4
UniRef50_A3GFA3 Cluster: Myc-family transcription factor; n=2; P... 31 4.4
UniRef50_Q6VU67 Cluster: Laminin alpha 3 splice variant b2; n=29... 31 5.9
UniRef50_Q2U9I6 Cluster: Predicted protein; n=1; Aspergillus ory... 31 5.9
UniRef50_Q9ET78 Cluster: Junctophilin-2; n=10; Euteleostomi|Rep:... 31 5.9
>UniRef50_UPI00015B63B2 Cluster: PREDICTED: similar to organic anion
transporter; n=1; Nasonia vitripennis|Rep: PREDICTED:
similar to organic anion transporter - Nasonia
vitripennis
Length = 992
Score = 39.9 bits (89), Expect = 0.013
Identities = 16/20 (80%), Positives = 18/20 (90%)
Frame = +2
Query: 173 VXTLMEFRLDSAEYCQAQHK 232
+ T MEF+LDSAEYCQAQHK
Sbjct: 973 ILTYMEFQLDSAEYCQAQHK 992
>UniRef50_Q4T1P0 Cluster: Chromosome undetermined SCAF10522, whole
genome shotgun sequence; n=2; Tetraodon nigroviridis|Rep:
Chromosome undetermined SCAF10522, whole genome shotgun
sequence - Tetraodon nigroviridis (Green puffer)
Length = 1540
Score = 34.3 bits (75), Expect = 0.63
Identities = 22/61 (36%), Positives = 27/61 (44%), Gaps = 1/61 (1%)
Frame = -2
Query: 329 SARGAXSHRRY-RQHGSSGWRSPALASRKHFLXICVEPGSTPRCPSGTPSMSPQKGSPDT 153
S R A ++R R G RSP A +H EP + PRC S P P+K P
Sbjct: 1318 SRRAAGGNQRATRAAGGRRRRSPGPAHPRHKGPAHEEPPTAPRCDSAPPPEGPEKEKPPG 1377
Query: 152 S 150
S
Sbjct: 1378 S 1378
>UniRef50_Q4QEE2 Cluster: Putative uncharacterized protein; n=1;
Leishmania major|Rep: Putative uncharacterized protein -
Leishmania major
Length = 845
Score = 33.1 bits (72), Expect = 1.5
Identities = 20/53 (37%), Positives = 28/53 (52%), Gaps = 3/53 (5%)
Frame = -2
Query: 296 RQHGSSGWRSPALASRKHFLXICVEPGSTPRCPSGTPSM---SPQKGSPDTSA 147
RQH S P+ R+ L + + P TP S +PS+ +PQ+ SP TSA
Sbjct: 344 RQHSRSDSLLPSTPGRQQQLAVALMPSFTPPHDSTSPSLRTPAPQQTSPCTSA 396
>UniRef50_A7S9N2 Cluster: Predicted protein; n=1; Nematostella
vectensis|Rep: Predicted protein - Nematostella vectensis
Length = 1363
Score = 33.1 bits (72), Expect = 1.5
Identities = 20/62 (32%), Positives = 32/62 (51%)
Frame = -2
Query: 329 SARGAXSHRRYRQHGSSGWRSPALASRKHFLXICVEPGSTPRCPSGTPSMSPQKGSPDTS 150
S R + R R+ S R P A R+H+ + + S+P P+ + SP++ SPD+S
Sbjct: 1205 SPRESLQQRTPRRSHSEVSRLPVKADRQHYSSLDL---SSPETPTSSDFPSPRRESPDSS 1261
Query: 149 AR 144
R
Sbjct: 1262 RR 1263
>UniRef50_Q4P4F6 Cluster: Putative uncharacterized protein; n=1;
Ustilago maydis|Rep: Putative uncharacterized protein -
Ustilago maydis (Smut fungus)
Length = 1215
Score = 33.1 bits (72), Expect = 1.5
Identities = 19/64 (29%), Positives = 28/64 (43%)
Frame = -2
Query: 341 GMWNSARGAXSHRRYRQHGSSGWRSPALASRKHFLXICVEPGSTPRCPSGTPSMSPQKGS 162
G + + GA H + + SSGW SPA+AS P P + P + + S
Sbjct: 1092 GSVSGSLGASPHAQAVRRASSGWTSPAVASAGAMRHWNTSPNLRPVADASLPHSTAHRPS 1151
Query: 161 PDTS 150
P +S
Sbjct: 1152 PWSS 1155
>UniRef50_A2Q2J0 Cluster: RNA-directed DNA polymerase (Reverse
transcriptase); Ribonuclease H; n=39; core
eudicotyledons|Rep: RNA-directed DNA polymerase (Reverse
transcriptase); Ribonuclease H - Medicago truncatula
(Barrel medic)
Length = 1146
Score = 31.9 bits (69), Expect = 3.4
Identities = 12/34 (35%), Positives = 22/34 (64%)
Frame = +2
Query: 158 LEIPSVXTLMEFRLDSAEYCQAQHKXTGSVSVRR 259
+EIPS+ +ME +L AE+CQ+++ + +R
Sbjct: 1022 VEIPSLRVIMEAKLSEAEWCQSRYDQLNLIEEKR 1055
>UniRef50_Q0AD47 Cluster: Twin-arginine translocation pathway signal
precursor; n=4; Proteobacteria|Rep: Twin-arginine
translocation pathway signal precursor - Nitrosomonas
eutropha (strain C71)
Length = 194
Score = 31.5 bits (68), Expect = 4.4
Identities = 12/25 (48%), Positives = 17/25 (68%)
Frame = +1
Query: 214 LPGSTQXNRKCFREARAGERHPLDP 288
+P + Q K R+ R+GERHP+DP
Sbjct: 74 IPEALQAIAKVLRDHRSGERHPIDP 98
>UniRef50_A3GFA3 Cluster: Myc-family transcription factor; n=2;
Pichia stipitis|Rep: Myc-family transcription factor -
Pichia stipitis (Yeast)
Length = 550
Score = 31.5 bits (68), Expect = 4.4
Identities = 14/25 (56%), Positives = 14/25 (56%)
Frame = -2
Query: 218 GSTPRCPSGTPSMSPQKGSPDTSAR 144
GS R P GTP M P K SP AR
Sbjct: 334 GSKRRTPHGTPIMHPNKNSPSIKAR 358
>UniRef50_Q6VU67 Cluster: Laminin alpha 3 splice variant b2; n=29;
cellular organisms|Rep: Laminin alpha 3 splice variant
b2 - Homo sapiens (Human)
Length = 3277
Score = 31.1 bits (67), Expect = 5.9
Identities = 22/64 (34%), Positives = 28/64 (43%), Gaps = 2/64 (3%)
Frame = +1
Query: 112 NFSFVL-LTLFCLAEVSG-DPFCGDIDGVPLGQRGVLPGSTQXNRKCFREARAGERHPLD 285
NF F L + +F ++ S DP GDI G GVLP + +C R G P
Sbjct: 463 NFPFCLRIPIFPVSTPSSEDPVAGDIKGCDCNLEGVLPEICDAHGRCL--CRPGVEGPRC 520
Query: 286 PCCR 297
CR
Sbjct: 521 DTCR 524
>UniRef50_Q2U9I6 Cluster: Predicted protein; n=1; Aspergillus
oryzae|Rep: Predicted protein - Aspergillus oryzae
Length = 193
Score = 31.1 bits (67), Expect = 5.9
Identities = 16/48 (33%), Positives = 24/48 (50%)
Frame = -2
Query: 293 QHGSSGWRSPALASRKHFLXICVEPGSTPRCPSGTPSMSPQKGSPDTS 150
+HG R A +RKH + +P +P SGTP + P + D+S
Sbjct: 25 RHGDQAERMLAAQARKHNVRPPTQPLPSPFSGSGTPMVPPAMANGDSS 72
>UniRef50_Q9ET78 Cluster: Junctophilin-2; n=10; Euteleostomi|Rep:
Junctophilin-2 - Mus musculus (Mouse)
Length = 696
Score = 31.1 bits (67), Expect = 5.9
Identities = 17/59 (28%), Positives = 25/59 (42%)
Frame = -2
Query: 341 GMWNSARGAXSHRRYRQHGSSGWRSPALASRKHFLXICVEPGSTPRCPSGTPSMSPQKG 165
G WN G R +G RSPA + +H ++P P PS P ++ +G
Sbjct: 503 GSWNGEPGGEGSRPATPSDGAGRRSPARPASEHMAIEALQP---PPAPSQEPEVAMYRG 558
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 304,599,025
Number of Sequences: 1657284
Number of extensions: 5024487
Number of successful extensions: 15869
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 15315
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 15858
length of database: 575,637,011
effective HSP length: 90
effective length of database: 426,481,451
effective search space used: 11941480628
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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