Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= e96h0818
         (740 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g02760.1 68416.m00268 histidyl-tRNA synthetase, putative / hi...   130   9e-31
At3g59410.1 68416.m06626 protein kinase family protein low simil...    40   0.001
At3g46100.1 68416.m04988 histidyl-tRNA synthetase / histidine--t...    32   0.35 
At2g06990.1 68415.m00800 HUA enhancer 2 (HEN2) / DExH-box RNA he...    29   4.3  
At1g59760.1 68414.m06729 ATP-dependent RNA helicase, putative si...    28   7.5  

>At3g02760.1 68416.m00268 histidyl-tRNA synthetase, putative /
           histidine--tRNA ligase, putative similar to SP|P12081
           Histidyl-tRNA synthetase (EC 6.1.1.21) (Histidine--tRNA
           ligase) (HisRS) {Homo sapiens}; contains Pfam profiles
           PF00587: tRNA synthetase class II core domain (G, H, P,
           S and T), PF03129: Anticodon binding domain
          Length = 479

 Score =  130 bits (314), Expect = 9e-31
 Identities = 56/83 (67%), Positives = 71/83 (85%)
 Frame = +3

Query: 6   KRYHIAKVYRRDNPAMTRGRYREFYQCDFDIAGIYDPMVPDAECLKVVTEILEALDIGQY 185
           KRY IAKVYRRDNP+  +GRYREFYQCDFDIAG+++PM PD E +K++TE+L+ L+IG Y
Sbjct: 134 KRYQIAKVYRRDNPS--KGRYREFYQCDFDIAGLFEPMGPDFEIVKILTELLDELEIGDY 191

Query: 186 ILKVNHRRLLDGMFEACGVPADK 254
            +K+NHR+LLDGM E CGVP +K
Sbjct: 192 EVKLNHRKLLDGMLEICGVPPEK 214



 Score = 47.2 bits (107), Expect = 1e-05
 Identities = 20/24 (83%), Positives = 24/24 (100%)
 Frame = +1

Query: 508 DKILFDLSLARGLDYYTGVIYEAV 579
           ++I+FDLSLARGLDYYTGVI+EAV
Sbjct: 302 ERIVFDLSLARGLDYYTGVIFEAV 325



 Score = 45.2 bits (102), Expect = 5e-05
 Identities = 19/38 (50%), Positives = 28/38 (73%)
 Frame = +2

Query: 278 DKLDKSPWEEVRTEMINEKGV*PDAADRIGEYVRLNGS 391
           DKLDK  +E+V+ EM+ EKG+  + ADRIG +V+  G+
Sbjct: 223 DKLDKQSFEQVKKEMVEEKGLSSEIADRIGNFVKEKGA 260



 Score = 37.9 bits (84), Expect = 0.007
 Identities = 19/28 (67%), Positives = 23/28 (82%), Gaps = 1/28 (3%)
 Frame = +2

Query: 629 SLG-GGRYDNLVGMFDSKNKQVPCVGVS 709
           S+G GGRYDNL+GMF +  KQVP VG+S
Sbjct: 333 SIGAGGRYDNLIGMFGT--KQVPAVGMS 358


>At3g59410.1 68416.m06626 protein kinase family protein low similarity
            to GCN2 eIF2alpha kinase [Mus musculus] GI:6066585;
            contains Pfam profiles PF03129: Anticodon binding domain,
            PF00069: Protein kinase domain
          Length = 1241

 Score = 40.3 bits (90), Expect = 0.001
 Identities = 29/83 (34%), Positives = 41/83 (49%), Gaps = 1/83 (1%)
 Frame = +3

Query: 6    KRYHIAKVYRRDNPAMTRGRYREFYQCDFDIAGIYDPMVPDAECLKVVTEI-LEALDIGQ 182
            KRY I+ VYRR   A+         Q DFDI G     + +AE LKV+ +I       G 
Sbjct: 868  KRYEISHVYRR---AIGHSPPNPCLQADFDIVG-GTLSLTEAEVLKVIVDITTHIFHRGS 923

Query: 183  YILKVNHRRLLDGMFEACGVPAD 251
              + +NH  LLD ++   G+ A+
Sbjct: 924  CDIHLNHGDLLDAIWSWAGIKAE 946


>At3g46100.1 68416.m04988 histidyl-tRNA synthetase / histidine--tRNA
           ligase identical to histidyl-tRNA synthetase
           [Arabidopsis thaliana] GI:3659909
          Length = 486

 Score = 32.3 bits (70), Expect = 0.35
 Identities = 12/20 (60%), Positives = 16/20 (80%)
 Frame = +1

Query: 514 ILFDLSLARGLDYYTGVIYE 573
           I FD S+ RGL YYTG+++E
Sbjct: 324 IQFDASVVRGLAYYTGIVFE 343


>At2g06990.1 68415.m00800 HUA enhancer 2 (HEN2) / DExH-box RNA
           helicase, putative nearly identical to HUA enhancer 2
           [Arabidopsis thaliana] GI:16024936
          Length = 995

 Score = 28.7 bits (61), Expect = 4.3
 Identities = 22/60 (36%), Positives = 31/60 (51%), Gaps = 4/60 (6%)
 Frame = +3

Query: 27  VYRRDNPAMTRGRYREFYQCDFDIAGIYDPMV---PDAECLKVVTEILEA-LDIGQYILK 194
           +Y     A++  +YRE  Q +F   G+    V   P+A CL + TEIL A L  G  +LK
Sbjct: 128 IYTSPLKALSNQKYREL-QHEFKDVGLMTGDVTLSPNASCLVMTTEILRAMLYRGSEVLK 186


>At1g59760.1 68414.m06729 ATP-dependent RNA helicase, putative
           similar to SP|P47047 ATP-dependent RNA helicase DOB1
           {Saccharomyces cerevisiae}, HUA enhancer 2 [Arabidopsis
           thaliana] GI:16024936; contains Pfam profiles PF00270:
           DEAD/DEAH box helicase, PF00271: Helicase conserved
           C-terminal domain
          Length = 988

 Score = 27.9 bits (59), Expect = 7.5
 Identities = 16/51 (31%), Positives = 28/51 (54%), Gaps = 3/51 (5%)
 Frame = +3

Query: 27  VYRRDNPAMTRGRYREFYQCDFDIAGIYDPMV---PDAECLKVVTEILEAL 170
           +Y     A++  +YR+F + +F   G+    V   P+A CL + TEIL ++
Sbjct: 114 IYTSPIKALSNQKYRDFKE-EFSDVGLMTGDVTIDPNASCLVMTTEILRSM 163


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,094,363
Number of Sequences: 28952
Number of extensions: 292634
Number of successful extensions: 731
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 713
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 730
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1633819784
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)