Bombyx BLAST Result against tribolium

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= e96h0817
         (666 letters)

Database: tribolium 
           336 sequences; 122,585 total letters

Searching.......................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY887136-1|AAW78361.1|  580|Tribolium castaneum vasa RNA helicas...    72   4e-15
DQ490059-1|ABF22614.1|  947|Tribolium castaneum short gastrulati...    21   6.9  

>AY887136-1|AAW78361.1|  580|Tribolium castaneum vasa RNA helicase
           protein.
          Length = 580

 Score = 72.1 bits (169), Expect = 4e-15
 Identities = 33/84 (39%), Positives = 48/84 (57%)
 Frame = +3

Query: 258 PIALVLAPTRELAQQIQQVAADFGHTSYVRNTCVFGGAPKREQARDLERGVEIVIATPGR 437
           P+ ++++PTRELA QI      F + S V+   ++GG     Q   +  G  I++ATPGR
Sbjct: 236 PVVVIMSPTRELAIQIADQGKKFAYNSTVKVAVIYGGTSTNHQRGRILGGCHILVATPGR 295

Query: 438 LIDFLEKGTTNLQRCTYLVLDEAD 509
           L DF+ +G  +     Y VLDEAD
Sbjct: 296 LKDFVNRGNVSFNSLKYFVLDEAD 319



 Score = 42.7 bits (96), Expect = 3e-06
 Identities = 20/58 (34%), Positives = 33/58 (56%)
 Frame = +2

Query: 2   VTVSGVEVHNPIQYFEEANFPDYVQQGVKTMGYKEPTPIQAQGWPIAMSGKNLVGVAK 175
           V V+G +   PI  FE +    ++ + VK  GY +PT IQ    P+ +SG++L+  A+
Sbjct: 146 VKVTGNDAPPPITSFETSGLRPHLLENVKKSGYTKPTAIQKYAIPVILSGRDLMSCAQ 203



 Score = 41.5 bits (93), Expect = 6e-06
 Identities = 25/64 (39%), Positives = 40/64 (62%), Gaps = 4/64 (6%)
 Frame = +2

Query: 446 FLGKGHNQLTAVHIFSS**G*RMLDMGFEPQIRKIIE-QIRP---DRQTLMWSATWPKEV 613
           F+ +G+    ++  F      RMLDMGF   + +++  Q  P   +RQTLM+SAT+P+EV
Sbjct: 299 FVNRGNVSFNSLKYFVLDEADRMLDMGFLGDVEEMLSHQSMPATGERQTLMFSATFPEEV 358

Query: 614 KKLA 625
           ++LA
Sbjct: 359 QQLA 362



 Score = 28.3 bits (60), Expect = 0.060
 Identities = 13/26 (50%), Positives = 19/26 (73%), Gaps = 2/26 (7%)
 Frame = +1

Query: 169 SQTGSGKTLAYILPAIVHI--NNQPP 240
           +QTGSGKT A++LP I ++  +  PP
Sbjct: 202 AQTGSGKTAAFMLPIIHNLLSDKNPP 227


>DQ490059-1|ABF22614.1|  947|Tribolium castaneum short gastrulation
           protein.
          Length = 947

 Score = 21.4 bits (43), Expect = 6.9
 Identities = 8/22 (36%), Positives = 13/22 (59%)
 Frame = -1

Query: 522 ISNIRQPHQELNMCTAVSWLCP 457
           +  +++PH ELNM    S + P
Sbjct: 345 VVRVKKPHYELNMVEVRSPVTP 366


  Database: tribolium
    Posted date:  Oct 23, 2007  1:18 PM
  Number of letters in database: 122,585
  Number of sequences in database:  336
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 173,065
Number of Sequences: 336
Number of extensions: 4143
Number of successful extensions: 7
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 7
length of database: 122,585
effective HSP length: 55
effective length of database: 104,105
effective search space used: 17281430
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)