Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= e96h0793
         (981 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC3F10.16c |||GTP binding protein, HSR1-related|Schizosaccharo...   109   6e-25
SPAC6F6.03c |||ribosome export GTPase|Schizosaccharomyces pombe|...    55   2e-08
SPBC26H8.08c |grn1||GTPase Grn1 |Schizosaccharomyces pombe|chr 2...    35   0.015
SPBC1706.03 |fzo1|SPBC839.01|mitochondrial fusion GTPase protein...    29   1.3  
SPBC1709.15c |cft2||cleavage factor two Cft2/polyadenylation fac...    28   1.7  
SPAC11E3.09 |pyp3||protein-tyrosine phosphatase Pyp3|Schizosacch...    27   4.0  
SPBC887.19 |rft1||human RFT1 ortholog |Schizosaccharomyces pombe...    27   4.0  
SPBC336.03 |efc25||exchange factor Cdc25p-like|Schizosaccharomyc...    27   5.3  
SPCC736.05 |wtf7||wtf element Wtf7|Schizosaccharomyces pombe|chr...    26   7.1  
SPAC27E2.07 |pvg2|mug53|galactose residue biosynthesis protein P...    26   7.1  
SPCC1827.07c ||SPCP1E11.01c|SPX/EXS domain protein|Schizosacchar...    26   7.1  
SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces ...    26   9.3  
SPBC1105.10 |rav1||RAVE complex subunit Rav1 |Schizosaccharomyce...    26   9.3  

>SPAC3F10.16c |||GTP binding protein,
           HSR1-related|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 616

 Score =  109 bits (262), Expect = 6e-25
 Identities = 52/104 (50%), Positives = 71/104 (68%), Gaps = 2/104 (1%)
 Frame = +3

Query: 507 LNELQAKLGAAVTPYERNLELWKQLWRTLEKSDIVLILLDARNPLLFRCVDLEKYAKE-- 680
           L +LQ   G  VTP+ERNLE+W+QLWR +E+SD+V+ ++DARNPL FR   LE+Y KE  
Sbjct: 140 LAQLQDVEGFIVTPFERNLEIWRQLWRVIERSDVVVQIVDARNPLFFRSAHLEQYVKEVG 199

Query: 681 QKCKSILLLNKADLTSEYERKCWG*IFHKENIRIVFFSGRQNYE 812
              K+ LL+NKAD+ +E +R  W   F++ NI  +FFS R   E
Sbjct: 200 PSKKNFLLVNKADMLTEEQRNYWSSYFNENNIPFLFFSARMAAE 243



 Score = 66.5 bits (155), Expect = 5e-12
 Identities = 37/93 (39%), Positives = 54/93 (58%), Gaps = 9/93 (9%)
 Frame = +1

Query: 256 QSVTAESSLQEFLSTAELAQREFTAEKLNLKYVKSVPSEVAIVTS---------QPDFDE 408
           +SVT E+ L EFL+TAEL + EF AEK N+  +++      +++          Q    +
Sbjct: 47  RSVTHETDLDEFLNTAELGEVEFIAEKQNVTVIQNPEQNPFLLSKEEAARSKQKQEKNKD 106

Query: 409 PLTVPRRPQWNPGVTAEEQLNRERETFLDWRRH 507
            LT+PRRP W+   TA E    ERE+FL+WRR+
Sbjct: 107 RLTIPRRPHWDQTTTAVELDRMERESFLNWRRN 139


>SPAC6F6.03c |||ribosome export GTPase|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 537

 Score = 54.8 bits (126), Expect = 2e-08
 Identities = 31/112 (27%), Positives = 55/112 (49%), Gaps = 2/112 (1%)
 Frame = +3

Query: 477 ERNILRLASALNELQAKLGAAVTPYERNLELWKQLWRTLEKSDIVLILLDARNPLLFRCV 656
           E  IL      +++      A+    ++  +W +L++ ++ SD+++ +LDAR+P+  RC 
Sbjct: 176 EERILANPDESDDVMLAARDAIFSKGQSKRIWNELYKVIDSSDVLIQVLDARDPVGTRCG 235

Query: 657 DLEKYAKEQKCKS--ILLLNKADLTSEYERKCWG*IFHKENIRIVFFSGRQN 806
            +E+Y + +      IL+LNK DL        W  I  KE   I F +   N
Sbjct: 236 TVERYLRNEASHKHMILVLNKVDLVPTSVAAAWVKILAKEYPTIAFHASINN 287


>SPBC26H8.08c |grn1||GTPase Grn1 |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 470

 Score = 35.1 bits (77), Expect = 0.015
 Identities = 19/62 (30%), Positives = 34/62 (54%), Gaps = 3/62 (4%)
 Frame = +3

Query: 573 KQLWRTLEKSDIVLILLDARNPLLFRCVDLEKY---AKEQKCKSILLLNKADLTSEYERK 743
           K+  + +E SD++L +LDAR+P   R  D+E+    +  ++ + I ++NK DL       
Sbjct: 154 KEFKKVVEASDVILYVLDARDPEGTRSKDVERQVLASSAEEKRLIFVINKIDLVPSEVLN 213

Query: 744 CW 749
            W
Sbjct: 214 KW 215


>SPBC1706.03 |fzo1|SPBC839.01|mitochondrial fusion GTPase
           protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 758

 Score = 28.7 bits (61), Expect = 1.3
 Identities = 13/49 (26%), Positives = 25/49 (51%)
 Frame = +3

Query: 594 EKSDIVLILLDARNPLLFRCVDLEKYAKEQKCKSILLLNKADLTSEYER 740
           E+ D+V+ +++A N       D  + A  +K    +++NK D   + ER
Sbjct: 289 EEIDVVVFVVNAENHFTLSATDFLRNASTEKSHIFIIVNKFDNIRDKER 337


>SPBC1709.15c |cft2||cleavage factor two Cft2/polyadenylation factor
           CPSF-73 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 797

 Score = 28.3 bits (60), Expect = 1.7
 Identities = 11/21 (52%), Positives = 15/21 (71%)
 Frame = -3

Query: 643 NKGFLASNNINTISDFSKVLH 581
           N+  L   NINTI+DFS++ H
Sbjct: 289 NENLLEFRNINTITDFSQISH 309


>SPAC11E3.09 |pyp3||protein-tyrosine phosphatase
           Pyp3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 303

 Score = 27.1 bits (57), Expect = 4.0
 Identities = 9/28 (32%), Positives = 17/28 (60%)
 Frame = +3

Query: 570 WKQLWRTLEKSDIVLILLDARNPLLFRC 653
           WK +W+++ KS I+++L   R     +C
Sbjct: 107 WKMVWQSVPKSGIIVMLTKLRERHRLKC 134


>SPBC887.19 |rft1||human RFT1 ortholog |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 527

 Score = 27.1 bits (57), Expect = 4.0
 Identities = 15/33 (45%), Positives = 21/33 (63%), Gaps = 4/33 (12%)
 Frame = -1

Query: 186 CFLLRFL----ENLSLMRALPRDSLFFLPIILT 100
           CF LRF+    ++ SL R+LPR  L  L I+L+
Sbjct: 439 CFALRFILHNYKDFSLPRSLPRPFLLALSILLS 471


>SPBC336.03 |efc25||exchange factor Cdc25p-like|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 987

 Score = 26.6 bits (56), Expect = 5.3
 Identities = 16/42 (38%), Positives = 25/42 (59%), Gaps = 3/42 (7%)
 Frame = -3

Query: 745 HFLSYSLVRSALFSNNILLHFCS-FAYFSRSTHL--NNKGFL 629
           + L ++L+++  F N I LHF     YFSR T L  +N+ F+
Sbjct: 261 YLLQHNLLQNFEFENPIKLHFDKIIPYFSRLTVLTFSNRAFI 302


>SPCC736.05 |wtf7||wtf element Wtf7|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 220

 Score = 26.2 bits (55), Expect = 7.1
 Identities = 22/83 (26%), Positives = 36/83 (43%), Gaps = 1/83 (1%)
 Frame = -3

Query: 754 QPQHFLSYSLVRSALFSNNILLHFCSFAYFSRSTHLNNKGFLASNNINTISDFSKVLH-N 578
           +P H+ S ++V   ++ NNI +       F++S   N     +SN +  +  +    H  
Sbjct: 47  EPPHYDSDAVVEMDMYENNIYMRTFKLRPFAKSGVTN-----SSNVVFQMKTYENNRHMQ 101

Query: 577 CFQSSKFLS*GVTAAPSLA*SSF 509
            F+   F   GVT    LA S F
Sbjct: 102 TFRLRPFAKNGVTNGVKLAQSLF 124


>SPAC27E2.07 |pvg2|mug53|galactose residue biosynthesis protein
           Pvg2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 389

 Score = 26.2 bits (55), Expect = 7.1
 Identities = 16/64 (25%), Positives = 33/64 (51%)
 Frame = +1

Query: 241 GTFESQSVTAESSLQEFLSTAELAQREFTAEKLNLKYVKSVPSEVAIVTSQPDFDEPLTV 420
           G + S+S+  + ++ +F+  AEL+ ++FT    +    +  P  +A  + Q DF   +  
Sbjct: 200 GVWMSRSLQLKKNIDKFVYAAELSVKQFTENITSSTVEEFQPIFMAPKSLQYDF--MIAT 257

Query: 421 PRRP 432
           P+ P
Sbjct: 258 PKHP 261


>SPCC1827.07c ||SPCP1E11.01c|SPX/EXS domain
           protein|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 682

 Score = 26.2 bits (55), Expect = 7.1
 Identities = 15/40 (37%), Positives = 22/40 (55%), Gaps = 1/40 (2%)
 Frame = -3

Query: 946 FNITPRKFGRNQXLQYLHL-QNWPYLCRXLFSFFTVSSRI 830
           +N+  RK GR Q  ++  L Q WPY+   + +F   SS I
Sbjct: 548 WNLLVRKDGRWQFREHRILKQLWPYIIAMILNFIVRSSFI 587


>SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 407

 Score = 25.8 bits (54), Expect = 9.3
 Identities = 13/46 (28%), Positives = 26/46 (56%)
 Frame = -1

Query: 216 VVWSMVISSTCFLLRFLENLSLMRALPRDSLFFLPIILTYTTKQGE 79
           +++  +ISS C LL  ++N S +  LP +  +   I+  +T++  E
Sbjct: 113 MIFENIISSLCLLLEAMDN-SNVSLLPENEKYRAVILRKHTSQPNE 157


>SPBC1105.10 |rav1||RAVE complex subunit Rav1 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1297

 Score = 25.8 bits (54), Expect = 9.3
 Identities = 14/43 (32%), Positives = 19/43 (44%)
 Frame = -2

Query: 374 TSLGTDFTYFRLSFSAVNSLCASSAVERNSWSEDSAVTDWDSN 246
           T L  D T     ++    LCASS  +  ++SED     W  N
Sbjct: 95  TQLSEDITQLSWGYNFDLLLCASSYTKLINFSEDKPRLVWSQN 137


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,119,158
Number of Sequences: 5004
Number of extensions: 87130
Number of successful extensions: 278
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 264
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 276
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 505288058
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)