Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0793
(981 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g27200.1 68415.m03269 GTP-binding family protein contains Pfa... 104 1e-22
At1g08410.1 68414.m00930 GTP-binding family protein contains Pfa... 96 3e-20
At1g52980.1 68414.m05995 GTP-binding family protein contains Pfa... 55 8e-08
At3g07050.1 68416.m00837 GTP-binding family protein contains Pfa... 48 1e-05
At4g02790.1 68417.m00379 GTP-binding family protein contains Pfa... 44 1e-04
At1g03160.1 68414.m00293 GTP-binding protein-related contains TI... 32 0.51
At5g37930.1 68418.m04569 seven in absentia (SINA) family protein... 31 1.2
At5g61040.1 68418.m07660 expressed protein 30 2.7
At2g44240.1 68415.m05505 expressed protein contains Pfam profil... 29 3.6
At5g08270.1 68418.m00974 expressed protein 29 4.7
>At2g27200.1 68415.m03269 GTP-binding family protein contains Pfam
domain, PF01926: GTPase of unknown function
Length = 537
Score = 104 bits (249), Expect = 1e-22
Identities = 46/100 (46%), Positives = 71/100 (71%), Gaps = 2/100 (2%)
Frame = +3
Query: 507 LNELQAKLGAAVTPYERNLELWKQLWRTLEKSDIVLILLDARNPLLFRCVDLEKYAKE-- 680
L L+ +TP+E+NL++W+QLWR LE+SD++++++DAR+PL +RC DLE YA+E
Sbjct: 137 LASLEENEKLVLTPFEKNLDIWRQLWRVLERSDLIVMVVDARDPLFYRCPDLEAYAQEID 196
Query: 681 QKCKSILLLNKADLTSEYERKCWG*IFHKENIRIVFFSGR 800
+ K++LL+NKADL Y R+ W F + NI VF+S +
Sbjct: 197 EHKKTMLLVNKADLLPSYVREKWAEYFSRNNILFVFWSAK 236
Score = 38.3 bits (85), Expect = 0.008
Identities = 15/31 (48%), Positives = 19/31 (61%)
Frame = +1
Query: 412 LTVPRRPQWNPGVTAEEQLNRERETFLDWRR 504
L VPRRP W P + E+ E++ FL WRR
Sbjct: 105 LQVPRRPHWTPKMNVEKLDANEKQAFLTWRR 135
>At1g08410.1 68414.m00930 GTP-binding family protein contains Pfam
domain, PF01926: GTPase of unknown function
Length = 589
Score = 96.3 bits (229), Expect = 3e-20
Identities = 43/87 (49%), Positives = 64/87 (73%), Gaps = 2/87 (2%)
Frame = +3
Query: 540 VTPYERNLELWKQLWRTLEKSDIVLILLDARNPLLFRCVDLEKYAKE--QKCKSILLLNK 713
+TP+E+NL++W+QLWR LE+SD++++++DAR+PL +RC DLE YA+E + K +LL+NK
Sbjct: 148 LTPFEKNLDIWRQLWRVLERSDLIVMVVDARDPLFYRCPDLEAYAQEIDEHKKIMLLVNK 207
Query: 714 ADLTSEYERKCWG*IFHKENIRIVFFS 794
ADL R+ W F NI VF+S
Sbjct: 208 ADLLPTDVREKWAEYFRLNNILFVFWS 234
Score = 40.7 bits (91), Expect = 0.001
Identities = 16/31 (51%), Positives = 21/31 (67%)
Frame = +1
Query: 412 LTVPRRPQWNPGVTAEEQLNRERETFLDWRR 504
L VPRRP W P ++ EE E++ FL+WRR
Sbjct: 105 LQVPRRPPWTPEMSVEELDANEKQAFLNWRR 135
Score = 29.1 bits (62), Expect = 4.7
Identities = 18/47 (38%), Positives = 29/47 (61%), Gaps = 7/47 (14%)
Frame = +2
Query: 110 MGKKNKESLGRALIKD-----RFSKNRNK--KHVEEXTMLHTTEVND 229
MGK K SLGR+L+K + SK++ K K++++ + TEV+D
Sbjct: 1 MGKSEKTSLGRSLVKHHNHMIQESKDKGKYYKNLQKKVLESVTEVSD 47
>At1g52980.1 68414.m05995 GTP-binding family protein contains Pfam
domain, PF01926: GTPase of unknown function
Length = 576
Score = 54.8 bits (126), Expect = 8e-08
Identities = 30/76 (39%), Positives = 45/76 (59%), Gaps = 2/76 (2%)
Frame = +3
Query: 567 LWKQLWRTLEKSDIVLILLDARNPLLFRCVDLEKYAKE-QKCK-SILLLNKADLTSEYER 740
+W +L++ ++ SD+++ ++DAR+P RC LEK KE K K ILLLNK DL +
Sbjct: 205 IWGELYKVIDSSDVIVQVIDARDPQGTRCHHLEKTLKEHHKHKHMILLLNKCDLVPAWAT 264
Query: 741 KCWG*IFHKENIRIVF 788
K W + KE + F
Sbjct: 265 KGWLRVLSKEYPTLAF 280
>At3g07050.1 68416.m00837 GTP-binding family protein contains Pfam
domain, PF01926: GTPase of unknown function
Length = 582
Score = 48.0 bits (109), Expect = 1e-05
Identities = 24/62 (38%), Positives = 37/62 (59%), Gaps = 2/62 (3%)
Frame = +3
Query: 570 WKQLWRTLEKSDIVLILLDARNPLLFRCVDLEKYAKEQKCKS--ILLLNKADLTSEYERK 743
+K+L + +E SD++L +LDAR+PL RC D+E+ + +LLLNK DL +
Sbjct: 127 YKELVKVIELSDVILEVLDARDPLGTRCTDMERMVMQAGPNKHLVLLLNKIDLVPREAAE 186
Query: 744 CW 749
W
Sbjct: 187 KW 188
>At4g02790.1 68417.m00379 GTP-binding family protein contains Pfam
domain, PF01926: GTPase of unknown function
Length = 372
Score = 44.4 bits (100), Expect = 1e-04
Identities = 24/76 (31%), Positives = 43/76 (56%)
Frame = +3
Query: 573 KQLWRTLEKSDIVLILLDARNPLLFRCVDLEKYAKEQKCKSILLLNKADLTSEYERKCWG 752
K+L L+ D+V+ + DAR PL ++ + +K IL+LN+ D+ S +R W
Sbjct: 108 KELREQLKLMDVVIEVRDARIPLSTTHPKMDAWLGNRK--RILVLNREDMISNDDRNDWA 165
Query: 753 *IFHKENIRIVFFSGR 800
F K+ I+++F +G+
Sbjct: 166 RYFAKQGIKVIFTNGK 181
>At1g03160.1 68414.m00293 GTP-binding protein-related contains
TIGRFAM TIGR00650: GTP-binding conserved hypothetical
protein domain; contains TIGRFAM TIGR00231: small
GTP-binding protein domain; similar to mitofusin 1
precursor (GI:12744896) [Homo sapiens]
Length = 642
Score = 32.3 bits (70), Expect = 0.51
Identities = 18/61 (29%), Positives = 32/61 (52%)
Frame = +3
Query: 597 KSDIVLILLDARNPLLFRCVDLEKYAKEQKCKSILLLNKADLTSEYERKCWG*IFHKENI 776
++D+++ +L A PL V +Y ++ K K + +LNK+D+ + F KEN
Sbjct: 447 RADLLVFVLSADRPLTESEVAFLRYTQQWKKKFVFILNKSDIYRDARELEEAISFVKENT 506
Query: 777 R 779
R
Sbjct: 507 R 507
>At5g37930.1 68418.m04569 seven in absentia (SINA) family protein
similar to SIAH1 protein [Brassica napus var. napus]
GI:7657876; contains Pfam profile PF03145: Seven in
absentia protein family
Length = 349
Score = 31.1 bits (67), Expect = 1.2
Identities = 13/32 (40%), Positives = 20/32 (62%)
Frame = -2
Query: 548 RCHCCTKLSLKFIQCRRQSKNVSLSRLSCSSA 453
RC CT L + +++CR K + SR+SC +A
Sbjct: 144 RCPSCT-LPIGYVRCRAMEKVIEASRVSCLNA 174
>At5g61040.1 68418.m07660 expressed protein
Length = 590
Score = 29.9 bits (64), Expect = 2.7
Identities = 15/40 (37%), Positives = 20/40 (50%)
Frame = +3
Query: 477 ERNILRLASALNELQAKLGAAVTPYERNLELWKQLWRTLE 596
E+ I L +AL E Q K+ V E + W +LW T E
Sbjct: 398 EKRIGELETALQESQRKVEQLVMESESKKKSWSRLWETRE 437
>At2g44240.1 68415.m05505 expressed protein contains Pfam profile
PF03080: Arabidopsis proteins of unknown function
Length = 402
Score = 29.5 bits (63), Expect = 3.6
Identities = 24/86 (27%), Positives = 41/86 (47%), Gaps = 11/86 (12%)
Frame = +1
Query: 283 QEFLSTAELAQREFTAEKLNLKYVKSVPSE------VAIVTSQPDFDEPL----TVPRRP 432
+EF E+ + F ++LN +KS+ SE ++TSQP FD PL T+ +P
Sbjct: 25 KEFFHHREIKVQRFL-KQLNKPALKSIKSEDGDIIDCVLITSQPAFDHPLLKNHTIQVKP 83
Query: 433 QWNP-GVTAEEQLNRERETFLDWRRH 507
+ P G +E + W+++
Sbjct: 84 SFIPEGEGDSTYTKKETKATQVWQKY 109
>At5g08270.1 68418.m00974 expressed protein
Length = 386
Score = 29.1 bits (62), Expect = 4.7
Identities = 15/37 (40%), Positives = 21/37 (56%)
Frame = +3
Query: 537 AVTPYERNLELWKQLWRTLEKSDIVLILLDARNPLLF 647
A T + ++WK L R LE S V +++ RNPL F
Sbjct: 246 ATTTENSSDKVWKMLSRPLEGSGGVEAVIEMRNPLAF 282
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 21,475,660
Number of Sequences: 28952
Number of extensions: 447253
Number of successful extensions: 1206
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1154
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1202
length of database: 12,070,560
effective HSP length: 81
effective length of database: 9,725,448
effective search space used: 2382734760
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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