Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0792
(728 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 118 1e-27
SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce... 42 7e-05
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 36 0.006
SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit... 28 1.6
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 28 1.6
SPBC1347.05c |||DNAJ domain protein Scj1|Schizosaccharomyces pom... 27 2.1
SPAC890.05 |||ribosome biogenesis protein|Schizosaccharomyces po... 27 3.6
SPAC24H6.01c ||SPAPB21F2.01|membrane bound O-acyltransferase, MB... 27 3.6
SPAC1D4.05c |||Erd1 homolog|Schizosaccharomyces pombe|chr 1|||Ma... 26 4.8
SPAC1006.04c |mcp3|mug7|sequence orphan|Schizosaccharomyces pomb... 26 6.3
SPAC1B2.03c |||GNS1/SUR4 family protein|Schizosaccharomyces pomb... 25 8.4
SPAC227.16c |||GINS complex subunit Psf3|Schizosaccharomyces pom... 25 8.4
SPAC1A6.09c |lag1||sphingosine N-acyltransferase Lag1|Schizosacc... 25 8.4
SPBC32H8.07 |git5|gpb1|heterotrimeric G protein beta subunit Git... 25 8.4
>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 190
Score = 118 bits (283), Expect = 1e-27
Identities = 50/84 (59%), Positives = 63/84 (75%)
Frame = +1
Query: 256 GKQNSKLKPEVLEDLKQNTEFSDAEIQEWYKGFLKDCPSGHLSVEEFKKIYGNFFPYGDA 435
GK SKL + L+DL ++T F E+Q+WYKGF KDCPSGHL+ EF+KIY FFP+GD
Sbjct: 2 GKSQSKLSQDQLQDLVRSTRFDKKELQQWYKGFFKDCPSGHLNKSEFQKIYKQFFPFGDP 61
Query: 436 SKFAEHVFRTFDANGDGTIDFREF 507
S FAE+VF FDA+ +G IDF+EF
Sbjct: 62 SAFAEYVFNVFDADKNGYIDFKEF 85
Score = 73.7 bits (173), Expect = 2e-14
Identities = 39/78 (50%), Positives = 48/78 (61%)
Frame = +3
Query: 495 FQRIPCALSVTSRGKLEQKLKWAFSMYDLDGNGYISRQEMLEIVTAIYQDGGAPL*RCRK 674
F+ CALSVTSRG+L KL WAF +YDLD NG IS EML IV AIY+ G+ + + +
Sbjct: 82 FKEFICALSVTSRGELNDKLIWAFQLYDLDNNGLISYDEMLRIVDAIYKMVGSMV-KLPE 140
Query: 675 MNLPPRNAPTKIFRQMDR 728
P KIF MD+
Sbjct: 141 DEDTPEKRVNKIFNMMDK 158
>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 174
Score = 42.3 bits (95), Expect = 7e-05
Identities = 22/44 (50%), Positives = 29/44 (65%), Gaps = 1/44 (2%)
Frame = +3
Query: 486 DYRFQRIPCALSVTS-RGKLEQKLKWAFSMYDLDGNGYISRQEM 614
D FQ +LSV S G E+KLK+AF +YD+D +GYIS E+
Sbjct: 72 DVDFQEFINSLSVFSVHGNKEEKLKFAFKIYDIDRDGYISNGEL 115
Score = 41.9 bits (94), Expect = 9e-05
Identities = 27/77 (35%), Positives = 40/77 (51%), Gaps = 2/77 (2%)
Frame = +1
Query: 283 EVLEDLKQNTEFSDAEIQEWYKGFLK-DC-PSGHLSVEEFKKIYGNFFPYGDASKFAEHV 456
++ EDL N+ FS+ EI+ K F+K D SG + EF I P ++ A +
Sbjct: 7 QIFEDLISNSSFSNEEIERIRKRFIKIDANQSGSIDRNEFLSI-----PSVASNPLASRL 61
Query: 457 FRTFDANGDGTIDFREF 507
F D +G G +DF+EF
Sbjct: 62 FSVVDEDGGGDVDFQEF 78
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 35.9 bits (79), Expect = 0.006
Identities = 12/31 (38%), Positives = 25/31 (80%)
Frame = +3
Query: 543 EQKLKWAFSMYDLDGNGYISRQEMLEIVTAI 635
E++++ AF ++D DGNGYI+ +E+ ++T++
Sbjct: 84 EEEVREAFKVFDKDGNGYITVEELTHVLTSL 114
Score = 28.7 bits (61), Expect = 0.90
Identities = 19/66 (28%), Positives = 34/66 (51%)
Frame = +1
Query: 310 TEFSDAEIQEWYKGFLKDCPSGHLSVEEFKKIYGNFFPYGDASKFAEHVFRTFDANGDGT 489
T+ AE +E + F +D G+++ E + + A++ + + DA+G+GT
Sbjct: 7 TDEQIAEFREAFSLFDRD-QDGNITSNELGVVMRSLGQSPTAAELQDMI-NEVDADGNGT 64
Query: 490 IDFREF 507
IDF EF
Sbjct: 65 IDFTEF 70
>SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 409
Score = 27.9 bits (59), Expect = 1.6
Identities = 16/57 (28%), Positives = 28/57 (49%), Gaps = 1/57 (1%)
Frame = +1
Query: 247 QNKGKQNSKLKPEVLEDLKQNTEFSDAEIQ-EWYKGFLKDCPSGHLSVEEFKKIYGN 414
+NK +K + ++ K+N AE + K L P+G +EE+K++Y N
Sbjct: 218 ENKEAAPAKKAEKKKDEKKKNAPKPQAEAPAKPPKHPLASAPNGSFDIEEYKRVYSN 274
>SPBC215.01 ||SPBC3B9.20|GTPase activating
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 834
Score = 27.9 bits (59), Expect = 1.6
Identities = 8/30 (26%), Positives = 21/30 (70%)
Frame = +3
Query: 546 QKLKWAFSMYDLDGNGYISRQEMLEIVTAI 635
+ + + F +YD +G+G++ + ++L++ AI
Sbjct: 615 RNISFIFELYDFNGDGFMDKPDVLKVSEAI 644
>SPBC1347.05c |||DNAJ domain protein Scj1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 381
Score = 27.5 bits (58), Expect = 2.1
Identities = 17/42 (40%), Positives = 23/42 (54%)
Frame = +1
Query: 214 KTTSANKAVIIQNKGKQNSKLKPEVLEDLKQNTEFSDAEIQE 339
KTTSA+ + I+ + K KLK + L+DL E D E E
Sbjct: 339 KTTSAHGSAYIEWEVKFPKKLKGKFLKDLNNLFEKYDNEFDE 380
>SPAC890.05 |||ribosome biogenesis protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 284
Score = 26.6 bits (56), Expect = 3.6
Identities = 18/53 (33%), Positives = 26/53 (49%)
Frame = +1
Query: 244 IQNKGKQNSKLKPEVLEDLKQNTEFSDAEIQEWYKGFLKDCPSGHLSVEEFKK 402
I K K+ K K + K+ + SD E ++ K LKD P SVE+ K+
Sbjct: 195 ITKKKKKEHKKKDKESSSKKRKSGSSDKEEKKKKKIKLKDKPESTSSVEKVKE 247
>SPAC24H6.01c ||SPAPB21F2.01|membrane bound O-acyltransferase, MBOAT
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 588
Score = 26.6 bits (56), Expect = 3.6
Identities = 24/84 (28%), Positives = 40/84 (47%), Gaps = 2/84 (2%)
Frame = -3
Query: 285 FRLEFTILFTLIL--DYYRFVRTCSFWIRIYNKN*HNRISIS*PSFLPLIFWTLSLSLVC 112
+RL F++LF L++ +V T + + +K+ N I F PL+ WTL +S+V
Sbjct: 154 YRLCFSLLFALLVYGTGVIYVLTIALINYLISKSLKNSI------FNPLLTWTLDISVVF 207
Query: 111 DYEAFYSFVLRKIHISLLIIFYHT 40
E F +H L + +T
Sbjct: 208 FKEYFAYCKFSSLHPGLGFLDQYT 231
>SPAC1D4.05c |||Erd1 homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 387
Score = 26.2 bits (55), Expect = 4.8
Identities = 11/24 (45%), Positives = 17/24 (70%)
Frame = -1
Query: 257 PLFWIITALFALVVFGYEFTIRTN 186
PL W+ITA F L+VF + + R++
Sbjct: 129 PLLWVITA-FILIVFPFPWRYRSS 151
>SPAC1006.04c |mcp3|mug7|sequence orphan|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 952
Score = 25.8 bits (54), Expect = 6.3
Identities = 13/32 (40%), Positives = 14/32 (43%)
Frame = +2
Query: 428 VTLVSSQNTYSAHSMRTATGL*ISENSVCT*C 523
VTL S N Y A S R L +S C C
Sbjct: 62 VTLTKSPNAYHASSAREEKDLQVSRRDTCFYC 93
>SPAC1B2.03c |||GNS1/SUR4 family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 334
Score = 25.4 bits (53), Expect = 8.4
Identities = 8/27 (29%), Positives = 16/27 (59%)
Frame = -2
Query: 109 LRSVLFVCIAKNSHFTSNNLLSHYIGF 29
+R+ LF C+ + HFT + +Y+ +
Sbjct: 115 MRNGLFYCVCDSRHFTQRLVTLYYLNY 141
>SPAC227.16c |||GINS complex subunit Psf3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 166
Score = 25.4 bits (53), Expect = 8.4
Identities = 13/23 (56%), Positives = 16/23 (69%), Gaps = 1/23 (4%)
Frame = -1
Query: 506 NSLKSI-VPSPFASNVRNTCSAN 441
NS SI +P+PF+S VRN AN
Sbjct: 53 NSFVSIHMPAPFSSVVRNALKAN 75
>SPAC1A6.09c |lag1||sphingosine N-acyltransferase
Lag1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 25.4 bits (53), Expect = 8.4
Identities = 9/22 (40%), Positives = 13/22 (59%), Gaps = 1/22 (4%)
Frame = -1
Query: 263 CLPLFWIIT-ALFALVVFGYEF 201
C P+FW+I F ++V Y F
Sbjct: 116 CFPIFWVIVFTAFRVIVMDYVF 137
>SPBC32H8.07 |git5|gpb1|heterotrimeric G protein beta subunit
Git5|Schizosaccharomyces pombe|chr 2|||Manual
Length = 305
Score = 25.4 bits (53), Expect = 8.4
Identities = 13/36 (36%), Positives = 17/36 (47%)
Frame = -1
Query: 332 ISASENSVFCFRSSNTSGLSLLFCLPLFWIITALFA 225
+SAS N V +TS F P WI+T F+
Sbjct: 37 LSASTNDVLLVWDLHTSNKVAFFEAPSVWIMTCAFS 72
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,338,971
Number of Sequences: 5004
Number of extensions: 76741
Number of successful extensions: 232
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 219
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 230
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 343230174
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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