Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0755
(791 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g18110.1 68415.m02105 elongation factor 1-beta, putative / EF... 87 1e-17
At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta id... 87 1e-17
At5g12110.1 68418.m01422 elongation factor 1B alpha-subunit 1 (e... 80 2e-15
At5g19510.1 68418.m02324 elongation factor 1B alpha-subunit 2 (e... 77 2e-14
At2g40660.1 68415.m05017 tRNA-binding region domain-containing p... 35 0.071
At5g20710.1 68418.m02459 beta-galactosidase, putative / lactase,... 34 0.12
At5g64960.1 68418.m08171 cyclin-dependent kinase, putative / CDK... 30 2.0
At2g05170.1 68415.m00544 vacuolar protein sorting 11 family prot... 28 6.2
At1g77760.1 68414.m09053 nitrate reductase 1 (NR1) identical to ... 28 6.2
At1g54970.1 68414.m06278 proline-rich family protein similar to ... 28 8.2
>At2g18110.1 68415.m02105 elongation factor 1-beta, putative /
EF-1-beta, putative nearly identical to eEF-1beta
[Arabidopsis thaliana] GI:398606
Length = 231
Score = 87.4 bits (207), Expect = 1e-17
Identities = 42/70 (60%), Positives = 51/70 (72%), Gaps = 3/70 (4%)
Frame = +1
Query: 508 ENQVRTIEMEGLLWGASKLVPVGYGINKLQIMCVIEDDKVSVDLLTEK---IPXXEDFVQ 678
E VR+I+MEGL WGASKLVPVGYGI KL IMC I DD VS+D + E+ + ++VQ
Sbjct: 162 EEAVRSIQMEGLFWGASKLVPVGYGIKKLHIMCTIVDDLVSIDTMIEEQLTVEPINEYVQ 221
Query: 679 SVDIAAFNQI 708
S DI AFN+I
Sbjct: 222 SCDIVAFNKI 231
Score = 50.0 bits (114), Expect = 2e-06
Identities = 20/58 (34%), Positives = 35/58 (60%)
Frame = +3
Query: 48 AVGDVKTAQGLNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLRWYNQI 221
A ++ + GL L+++L +SY++GY S+ D+ VF + K P + +V RW+N I
Sbjct: 3 AFPNLNSGSGLKKLDEHLLTRSYITGYQASKDDITVFTALSKPPTSEFVNVSRWFNHI 60
Score = 45.6 bits (103), Expect = 4e-05
Identities = 19/36 (52%), Positives = 27/36 (75%)
Frame = +2
Query: 404 KAYADKKSKKPALIAKSSILLDVKPWDDETDMKEMK 511
+A + K S K KSS+L+D+KPWDDETDMK+++
Sbjct: 127 RAASVKASTKKKESGKSSVLMDIKPWDDETDMKKLE 162
>At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta
identical to SP|P48006 Elongation factor 1-beta
(EF-1-beta) {Arabidopsis thaliana}
Length = 231
Score = 87.0 bits (206), Expect = 1e-17
Identities = 41/70 (58%), Positives = 52/70 (74%), Gaps = 3/70 (4%)
Frame = +1
Query: 508 ENQVRTIEMEGLLWGASKLVPVGYGINKLQIMCVIEDDKVSVDLLTEK---IPXXEDFVQ 678
E V++I+MEGL WGASKLVPVGYGI KLQI+C I DD VS+D + E+ + ++VQ
Sbjct: 162 EEAVKSIQMEGLFWGASKLVPVGYGIKKLQILCTIVDDLVSIDTMIEEQLTVEPINEYVQ 221
Query: 679 SVDIAAFNQI 708
S DI AFN+I
Sbjct: 222 SCDIVAFNKI 231
Score = 48.4 bits (110), Expect = 5e-06
Identities = 20/58 (34%), Positives = 34/58 (58%)
Frame = +3
Query: 48 AVGDVKTAQGLNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLRWYNQI 221
A ++ + GL L+++L +SY++GY S+ D+ VF + K P + + RWYN I
Sbjct: 3 AFPNLNSDAGLKKLDEHLLTRSYITGYQASKDDITVFAALAKPPTSQYVNASRWYNHI 60
Score = 45.6 bits (103), Expect = 4e-05
Identities = 19/36 (52%), Positives = 27/36 (75%)
Frame = +2
Query: 404 KAYADKKSKKPALIAKSSILLDVKPWDDETDMKEMK 511
+A + K S K KSS+L+D+KPWDDETDMK+++
Sbjct: 127 RAASVKASTKKKESGKSSVLIDIKPWDDETDMKKLE 162
>At5g12110.1 68418.m01422 elongation factor 1B alpha-subunit 1
(eEF1Balpha1) identical to elongation factor 1B
alpha-subunit [Arabidopsis thaliana] GI:6686819
Length = 228
Score = 79.8 bits (188), Expect = 2e-15
Identities = 42/70 (60%), Positives = 49/70 (70%), Gaps = 3/70 (4%)
Frame = +1
Query: 508 ENQVRTIEMEGLLWGASKLVPVGYGINKLQIMCVIEDDKVSVDLLTEKIPXXE---DFVQ 678
E VR+++M GL WGASKLVPVGYGI KL IM I DD VSVD L E E +++Q
Sbjct: 159 EEAVRSVQMPGLTWGASKLVPVGYGIKKLTIMMTIVDDLVSVDNLIEDHLTSEPNNEYIQ 218
Query: 679 SVDIAAFNQI 708
SVDI AFN+I
Sbjct: 219 SVDIVAFNKI 228
Score = 49.6 bits (113), Expect = 2e-06
Identities = 22/58 (37%), Positives = 36/58 (62%)
Frame = +3
Query: 57 DVKTAQGLNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLRWYNQIASY 230
D+ T +GL L ++LA K+Y+SG S DV+V+ V + P P+ +WY+ +AS+
Sbjct: 7 DLHTERGLKTLEEHLAGKTYISGDQLSVDDVKVYAAVLENPGDGFPNASKWYDSVASH 64
Score = 46.8 bits (106), Expect = 2e-05
Identities = 20/33 (60%), Positives = 26/33 (78%)
Frame = +2
Query: 413 ADKKSKKPALIAKSSILLDVKPWDDETDMKEMK 511
A K +KK KSS+LL+VKPWDDETDMK+++
Sbjct: 127 AKKDTKKTKESGKSSVLLEVKPWDDETDMKKLE 159
>At5g19510.1 68418.m02324 elongation factor 1B alpha-subunit 2
(eEF1Balpha2) identical to elongation factor 1B
alpha-subunit [Arabidopsis thaliana] GI:6686821
Length = 224
Score = 76.6 bits (180), Expect = 2e-14
Identities = 41/70 (58%), Positives = 46/70 (65%), Gaps = 3/70 (4%)
Frame = +1
Query: 508 ENQVRTIEMEGLLWGASKLVPVGYGINKLQIMCVIEDDKVSVDLLTEKIPXXE---DFVQ 678
E VR +EM GL WGASKLVPVGYGI KL IM I DD VS D L E E +++Q
Sbjct: 155 EEAVRGVEMPGLFWGASKLVPVGYGIKKLTIMFTIVDDLVSPDNLIEDFLTSEPNNEYIQ 214
Query: 679 SVDIAAFNQI 708
S DI AFN+I
Sbjct: 215 SCDIVAFNKI 224
Score = 50.8 bits (116), Expect = 1e-06
Identities = 21/33 (63%), Positives = 27/33 (81%)
Frame = +2
Query: 413 ADKKSKKPALIAKSSILLDVKPWDDETDMKEMK 511
A K +KKP KSS+L+DVKPWDDETDMK+++
Sbjct: 123 AKKDTKKPKESGKSSVLMDVKPWDDETDMKKLE 155
Score = 46.4 bits (105), Expect = 2e-05
Identities = 20/57 (35%), Positives = 34/57 (59%)
Frame = +3
Query: 57 DVKTAQGLNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLRWYNQIAS 227
D+ T +G+ + ++LA K+Y+SG S DV+V+ V P+ P+ +WY +AS
Sbjct: 7 DLHTEEGVKSVEEHLAGKTYISGDQLSVDDVKVYAAVPVKPSDAFPNASKWYESVAS 63
>At2g40660.1 68415.m05017 tRNA-binding region domain-containing
protein similar to SP|Q12904 Multisynthetase complex
auxiliary component p43 [Contains: Endothelial-monocyte
activating polypeptide II (EMAP-II) (Small inducible
cytokine subfamily E member 1)] {Homo sapiens}; contains
Pfam profile PF01588: Putative tRNA binding domain
Length = 389
Score = 34.7 bits (76), Expect = 0.071
Identities = 23/57 (40%), Positives = 29/57 (50%), Gaps = 9/57 (15%)
Frame = +3
Query: 78 LNDLNQYLAEKSYV--SGYTPSQADVQVFEQV-------GKAPAANLPHVLRWYNQI 221
L LN LA KS + +G TPS ADV VF + + +PHV+RW N I
Sbjct: 76 LEKLNLELATKSVLLGNGLTPSAADVAVFSALHSSVLGLSDSDKEKVPHVIRWVNYI 132
>At5g20710.1 68418.m02459 beta-galactosidase, putative / lactase,
putative strong similarity to beta-galactosidase
precursor (EC 3.2.1.23) (Lactase) SP:P49676 from
[Brassica oleracea]
Length = 769
Score = 33.9 bits (74), Expect = 0.12
Identities = 16/54 (29%), Positives = 29/54 (53%), Gaps = 1/54 (1%)
Frame = -2
Query: 316 FRCWGGSRGFSTGGQWAGALSQVLRSAGVYEAI*LYHRST-WGRLAAGALPTCS 158
F+ WGG + T A ++++ ++ G ++ +YH T +GR+A G T S
Sbjct: 202 FKNWGGKHPYRTAEDLAFSVARFFQTGGTFQNYYMYHGGTNFGRVAGGPYITTS 255
>At5g64960.1 68418.m08171 cyclin-dependent kinase, putative / CDK,
putative similar to cyclin dependent kinase C
[Lycopersicon esculentum] gi|15215944|emb|CAC51391
Length = 513
Score = 29.9 bits (64), Expect = 2.0
Identities = 16/54 (29%), Positives = 28/54 (51%), Gaps = 2/54 (3%)
Frame = +3
Query: 93 QYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLR--WYNQIASYTPAERK 248
+ L K + G T ++ +++E G +N P V + WYNQ+ S P +R+
Sbjct: 233 ELLNGKPILPGKTENEQLNKIYELCGSPDESNWPGVSKMPWYNQMKSSRPLKRR 286
>At2g05170.1 68415.m00544 vacuolar protein sorting 11 family protein
/ VPS11 family protein similar to Vacuolar protein
sorting 11 (hVPS11) (PP3476) (Swiss-Prot:Q9H270) [Homo
sapiens]; similar to Vacuolar biogenesis protein END1
(PEP5 protein) (Vacuolar protein sorting 11)
(Swiss-Prot:P12868) [Saccharomyces cerevisiae]
Length = 932
Score = 28.3 bits (60), Expect = 6.2
Identities = 16/32 (50%), Positives = 17/32 (53%)
Frame = +3
Query: 78 LNDLNQYLAEKSYVSGYTPSQADVQVFEQVGK 173
L DL Y YVS PSQA V + EQ GK
Sbjct: 495 LEDLGNYDEALQYVSSLEPSQAGVTI-EQYGK 525
>At1g77760.1 68414.m09053 nitrate reductase 1 (NR1) identical to
SP|P11832 Nitrate reductase 1 (formerly EC 1.6.6.1)
(NR1){Arabidopsis thaliana}
Length = 917
Score = 28.3 bits (60), Expect = 6.2
Identities = 21/58 (36%), Positives = 28/58 (48%), Gaps = 1/58 (1%)
Frame = -2
Query: 397 FFSNSFSFCILFLVTRTK*INVVXIVVFRCWGGSRGFSTGGQWAGA-LSQVLRSAGVY 227
F S F ++ R K N+V + WG S G ST W G LS++LR G+Y
Sbjct: 186 FPSREFPVTLVCAGNRRKEQNMVKQTIGFNWG-SAGVSTS-LWKGIPLSEILRRCGIY 241
>At1g54970.1 68414.m06278 proline-rich family protein similar to
proline-rich protein GI:170048 from [Glycine max]
Length = 335
Score = 27.9 bits (59), Expect = 8.2
Identities = 15/34 (44%), Positives = 17/34 (50%), Gaps = 1/34 (2%)
Frame = +3
Query: 228 YTPAERKTWLRAPAH*PPVLNPRL-PPQQRKTTM 326
YTP K L P + PPV P L PP K T+
Sbjct: 47 YTPPVHKPTLPPPVYTPPVHKPTLSPPVYTKPTL 80
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,899,130
Number of Sequences: 28952
Number of extensions: 294946
Number of successful extensions: 799
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 773
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 794
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1785055200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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