Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0743
(603 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 157 1e-39
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 157 2e-39
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 157 2e-39
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 95 5e-21
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 88 8e-19
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 73 2e-14
SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 38 0.001
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 36 0.006
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 36 0.006
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 35 0.008
SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosacch... 33 0.024
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 31 0.098
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 31 0.13
SPAC56F8.03 |||translation initiation factor IF2 |Schizosaccharo... 29 0.52
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 29 0.52
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 27 1.6
SPBC405.01 |ade1|min4, SPBC4C3.02c|phosphoribosylamine-glycine l... 26 3.7
SPBC646.09c |int6|yin6|translation initiation factor eIF3e|Schiz... 26 4.9
SPBC1703.06 |pof10||F-box protein Pof10|Schizosaccharomyces pomb... 25 6.4
SPAC12G12.01c ||SPAC630.02|ubiquitin-protein ligase E3|Schizosac... 25 6.4
SPAC9G1.11c |spn4||septin Spn4|Schizosaccharomyces pombe|chr 1||... 25 8.5
SPBC1703.13c |||inorganic phosphate transporter |Schizosaccharom... 25 8.5
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 157 bits (381), Expect = 1e-39
Identities = 73/84 (86%), Positives = 78/84 (92%)
Frame = +1
Query: 256 IALWKFETSKYYVTIIDAPGHXDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTR 435
IALWKFET KY VT+IDAPGH DFIKNMITGTSQADCAVLI+ GTGEFEAGISK+GQTR
Sbjct: 75 IALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAVLIIGGGTGEFEAGISKDGQTR 134
Query: 436 EHALLAFTLGVKQLIVGVNKMDST 507
EHALLA+TLGVKQLIV VNKMD+T
Sbjct: 135 EHALLAYTLGVKQLIVAVNKMDTT 158
Score = 141 bits (341), Expect = 8e-35
Identities = 64/68 (94%), Positives = 66/68 (97%)
Frame = +3
Query: 36 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 215
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GKGSFKYAWVL
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60
Query: 216 DKLKAERE 239
DKLKAERE
Sbjct: 61 DKLKAERE 68
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 157 bits (380), Expect = 2e-39
Identities = 72/84 (85%), Positives = 78/84 (92%)
Frame = +1
Query: 256 IALWKFETSKYYVTIIDAPGHXDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTR 435
IALWKFET KY VT+IDAPGH DFIKNMITGTSQADCA+LI+ GTGEFEAGISK+GQTR
Sbjct: 75 IALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAILIIGGGTGEFEAGISKDGQTR 134
Query: 436 EHALLAFTLGVKQLIVGVNKMDST 507
EHALLA+TLGVKQLIV VNKMD+T
Sbjct: 135 EHALLAYTLGVKQLIVAVNKMDTT 158
Score = 141 bits (341), Expect = 8e-35
Identities = 64/68 (94%), Positives = 66/68 (97%)
Frame = +3
Query: 36 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 215
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GKGSFKYAWVL
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60
Query: 216 DKLKAERE 239
DKLKAERE
Sbjct: 61 DKLKAERE 68
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 157 bits (380), Expect = 2e-39
Identities = 72/84 (85%), Positives = 78/84 (92%)
Frame = +1
Query: 256 IALWKFETSKYYVTIIDAPGHXDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTR 435
IALWKFET KY VT+IDAPGH DFIKNMITGTSQADCA+LI+ GTGEFEAGISK+GQTR
Sbjct: 75 IALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAILIIGGGTGEFEAGISKDGQTR 134
Query: 436 EHALLAFTLGVKQLIVGVNKMDST 507
EHALLA+TLGVKQLIV VNKMD+T
Sbjct: 135 EHALLAYTLGVKQLIVAVNKMDTT 158
Score = 141 bits (341), Expect = 8e-35
Identities = 64/68 (94%), Positives = 66/68 (97%)
Frame = +3
Query: 36 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 215
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GKGSFKYAWVL
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60
Query: 216 DKLKAERE 239
DKLKAERE
Sbjct: 61 DKLKAERE 68
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 95.5 bits (227), Expect = 5e-21
Identities = 42/77 (54%), Positives = 55/77 (71%)
Frame = +1
Query: 271 FETSKYYVTIIDAPGHXDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALL 450
FET +++DAPGH ++ NMI G SQAD VL+++A GEFEAG + GQTREHA+L
Sbjct: 311 FETEHRRFSLLDAPGHKGYVTNMINGASQADIGVLVISARRGEFEAGFERGGQTREHAVL 370
Query: 451 AFTLGVKQLIVGVNKMD 501
A T G+ L+V +NKMD
Sbjct: 371 ARTQGINHLVVVINKMD 387
Score = 80.6 bits (190), Expect = 2e-16
Identities = 35/64 (54%), Positives = 47/64 (73%)
Frame = +3
Query: 48 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLK 227
K H+NIV IGHVD+GKST G++++ G +DKRT+EK E+EA+E GK S+ +W LD
Sbjct: 236 KEHVNIVFIGHVDAGKSTLGGNILFLTGMVDKRTMEKIEREAKEAGKESWYLSWALDSTS 295
Query: 228 AERE 239
ERE
Sbjct: 296 EERE 299
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 88.2 bits (209), Expect = 8e-19
Identities = 42/82 (51%), Positives = 52/82 (63%)
Frame = +1
Query: 256 IALWKFETSKYYVTIIDAPGHXDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTR 435
+A FE+ K I DAPGH DFI MI G S AD AVL+V + FE G +NGQTR
Sbjct: 245 VASTTFESDKKIYEIGDAPGHRDFISGMIAGASSADFAVLVVDSSQNNFERGFLENGQTR 304
Query: 436 EHALLAFTLGVKQLIVGVNKMD 501
EHA L LG+ +++V VNK+D
Sbjct: 305 EHAYLLRALGISEIVVSVNKLD 326
Score = 72.5 bits (170), Expect = 4e-14
Identities = 31/63 (49%), Positives = 44/63 (69%)
Frame = +3
Query: 48 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLK 227
K +++VV GHVDSGKST G ++++ G I+ R+++K EA GKGSF YAW+LD +
Sbjct: 175 KPVVHLVVTGHVDSGKSTMLGRIMFELGEINSRSMQKLHNEAANSGKGSFSYAWLLDTTE 234
Query: 228 AER 236
ER
Sbjct: 235 EER 237
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 73.3 bits (172), Expect = 2e-14
Identities = 35/81 (43%), Positives = 53/81 (65%)
Frame = +1
Query: 268 KFETSKYYVTIIDAPGHXDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHAL 447
++ET+ + +D PGH D+IKNMITG + D A+++V+A G+ QTREH L
Sbjct: 110 EYETANRHYAHVDCPGHADYIKNMITGAATMDGAIIVVSATDGQMP-------QTREHLL 162
Query: 448 LAFTLGVKQLIVGVNKMDSTD 510
LA +GVKQ++V +NK+D +
Sbjct: 163 LARQVGVKQIVVYINKVDMVE 183
Score = 35.1 bits (77), Expect = 0.008
Identities = 16/29 (55%), Positives = 20/29 (68%)
Frame = +3
Query: 42 KEKTHINIVVIGHVDSGKSTTTGHLIYKC 128
++K H+NI IGHVD GK+T T I KC
Sbjct: 49 RKKPHVNIGTIGHVDHGKTTLTA-AITKC 76
>SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 446
Score = 37.5 bits (83), Expect = 0.001
Identities = 20/71 (28%), Positives = 34/71 (47%)
Frame = +1
Query: 289 YVTIIDAPGHXDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGV 468
+V+ +D PGH + M+ G + D A+L++A QT EH + +
Sbjct: 109 HVSFVDCPGHDILMATMLNGAAVMDAALLLIAGNES------CPQPQTSEHLAAIEIMQL 162
Query: 469 KQLIVGVNKMD 501
K +I+ NK+D
Sbjct: 163 KHIIILQNKVD 173
Score = 25.0 bits (52), Expect = 8.5
Identities = 12/24 (50%), Positives = 14/24 (58%)
Frame = +3
Query: 30 PKMGKEKTHINIVVIGHVDSGKST 101
P + + INI IGHV GKST
Sbjct: 15 PAIISRQATINIGTIGHVAHGKST 38
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 35.5 bits (78), Expect = 0.006
Identities = 17/32 (53%), Positives = 19/32 (59%)
Frame = +3
Query: 36 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCG 131
MGK N+ VI HVD GKST T L+ K G
Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAG 44
Score = 28.7 bits (61), Expect = 0.69
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = +1
Query: 286 YYVTIIDAPGHXDFIKNMITGTSQADCAVLIV 381
+ V +ID+PGH DF + D A+++V
Sbjct: 98 FLVNLIDSPGHVDFSSEVTAALRVTDGALVVV 129
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 35.5 bits (78), Expect = 0.006
Identities = 17/32 (53%), Positives = 19/32 (59%)
Frame = +3
Query: 36 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCG 131
MGK N+ VI HVD GKST T L+ K G
Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAG 44
Score = 28.7 bits (61), Expect = 0.69
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = +1
Query: 286 YYVTIIDAPGHXDFIKNMITGTSQADCAVLIV 381
+ V +ID+PGH DF + D A+++V
Sbjct: 98 FLVNLIDSPGHVDFSSEVTAALRVTDGALVVV 129
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 35.1 bits (77), Expect = 0.008
Identities = 21/57 (36%), Positives = 29/57 (50%)
Frame = +1
Query: 271 FETSKYYVTIIDAPGHXDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREH 441
FE S Y + IID PGH DF + D AVL++ A +G I+ + Q R +
Sbjct: 141 FEKS-YNINIIDTPGHIDFTIEVERALRVLDGAVLVLCAVSGVQSQTITVDRQMRRY 196
Score = 29.1 bits (62), Expect = 0.52
Identities = 12/26 (46%), Positives = 17/26 (65%)
Frame = +3
Query: 60 NIVVIGHVDSGKSTTTGHLIYKCGGI 137
NI + H+DSGK+T T ++Y G I
Sbjct: 61 NIGISAHIDSGKTTFTERVLYYTGRI 86
>SPBC1271.15c |||translation initiation factor
IF-2Mt|Schizosaccharomyces pombe|chr 2|||Manual
Length = 686
Score = 33.5 bits (73), Expect = 0.024
Identities = 15/42 (35%), Positives = 21/42 (50%)
Frame = +1
Query: 271 FETSKYYVTIIDAPGHXDFIKNMITGTSQADCAVLIVAAGTG 396
F+ ++T +D PGH F G + AD VL+VA G
Sbjct: 215 FDKGSKFITFLDTPGHMAFEAMRKRGANIADIVVLVVAGDDG 256
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 31.5 bits (68), Expect = 0.098
Identities = 12/45 (26%), Positives = 23/45 (51%)
Frame = +1
Query: 262 LWKFETSKYYVTIIDAPGHXDFIKNMITGTSQADCAVLIVAAGTG 396
++ + Y + +ID PGH DF ++ + + +L+V A G
Sbjct: 116 IYYYHGQSYLLNLIDTPGHVDFRAEVMHSLAACEGCILLVDASQG 160
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1000
Score = 31.1 bits (67), Expect = 0.13
Identities = 13/37 (35%), Positives = 21/37 (56%)
Frame = +1
Query: 286 YYVTIIDAPGHXDFIKNMITGTSQADCAVLIVAAGTG 396
Y + +ID+PGH DF + + + D A ++V A G
Sbjct: 94 YLINLIDSPGHVDFSSEVSSASRLCDGAFVLVDAVEG 130
>SPAC56F8.03 |||translation initiation factor IF2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1079
Score = 29.1 bits (62), Expect = 0.52
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +1
Query: 298 IIDAPGHXDFIKNMITGTSQADCAVLIV 381
IID PGH F GTS + A+L++
Sbjct: 553 IIDTPGHESFTNLRSRGTSLCNIAILVI 580
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 29.1 bits (62), Expect = 0.52
Identities = 23/70 (32%), Positives = 34/70 (48%)
Frame = +1
Query: 292 VTIIDAPGHXDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVK 471
+ +ID PGH DF + + D AV I+ + AG+ QT+ A G+
Sbjct: 95 INLIDTPGHADFTFEVERSVAVLDGAVAII-----DGSAGV--EAQTKVVWKQATKRGIP 147
Query: 472 QLIVGVNKMD 501
++I VNKMD
Sbjct: 148 KVIF-VNKMD 156
Score = 28.7 bits (61), Expect = 0.69
Identities = 10/24 (41%), Positives = 17/24 (70%)
Frame = +3
Query: 60 NIVVIGHVDSGKSTTTGHLIYKCG 131
N+ +I H+D+GK+T T ++Y G
Sbjct: 30 NVGIIAHIDAGKTTLTEKMLYYGG 53
>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 983
Score = 27.5 bits (58), Expect = 1.6
Identities = 11/27 (40%), Positives = 16/27 (59%)
Frame = +1
Query: 301 IDAPGHXDFIKNMITGTSQADCAVLIV 381
ID PGH DF+ + + +D VL+V
Sbjct: 214 IDTPGHVDFVDEVAAPMAISDGVVLVV 240
>SPBC405.01 |ade1|min4, SPBC4C3.02c|phosphoribosylamine-glycine
ligase |Schizosaccharomyces pombe|chr 2|||Manual
Length = 788
Score = 26.2 bits (55), Expect = 3.7
Identities = 16/45 (35%), Positives = 21/45 (46%)
Frame = -1
Query: 285 LASFELPESNTIVIPTHAQPLVCPIPKHI*RILYPFPGPPSRTSR 151
L F L + +VIP PLV I H R+ P GP + +R
Sbjct: 62 LVKFALDKDVNLVIPGPELPLVEGIEGHFRRVGIPCFGPSALAAR 106
>SPBC646.09c |int6|yin6|translation initiation factor
eIF3e|Schizosaccharomyces pombe|chr 2|||Manual
Length = 501
Score = 25.8 bits (54), Expect = 4.9
Identities = 12/48 (25%), Positives = 28/48 (58%)
Frame = -3
Query: 277 FRTSREQYDCDTYSRSAFSLSNTQAYLKDPLPISWASFSNFSMVRLSI 134
++ ++ QY+C Y ++ L + +A+ KDP + A++ F+ L++
Sbjct: 145 YKFAQFQYNCGNYGGASDLLYHFRAFSKDPELNASATWGKFASEILTV 192
>SPBC1703.06 |pof10||F-box protein Pof10|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 662
Score = 25.4 bits (53), Expect = 6.4
Identities = 15/43 (34%), Positives = 19/43 (44%)
Frame = -1
Query: 222 VCPIPKHI*RILYPFPGPPSRTSRWYVCQYHHICRSSDQWWWT 94
V +PK I I++ F P S S C+Y S D W T
Sbjct: 31 VLNLPKEILIIIFSFLDPRSLLSAQCTCKYWKKLLSDDLSWRT 73
>SPAC12G12.01c ||SPAC630.02|ubiquitin-protein ligase
E3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 905
Score = 25.4 bits (53), Expect = 6.4
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = -1
Query: 297 GNIVLASFELPESNTIVIPTHAQP 226
GNI L S + PE +V P +A+P
Sbjct: 67 GNISLLSVKHPEFQAVVTPGYARP 90
>SPAC9G1.11c |spn4||septin Spn4|Schizosaccharomyces pombe|chr
1|||Manual
Length = 380
Score = 25.0 bits (52), Expect = 8.5
Identities = 9/15 (60%), Positives = 11/15 (73%)
Frame = +1
Query: 292 VTIIDAPGHXDFIKN 336
+T+ID PG DFI N
Sbjct: 89 LTVIDTPGFGDFINN 103
>SPBC1703.13c |||inorganic phosphate transporter
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 311
Score = 25.0 bits (52), Expect = 8.5
Identities = 8/19 (42%), Positives = 12/19 (63%)
Frame = -1
Query: 177 PGPPSRTSRWYVCQYHHIC 121
P PP +T + Y QY+ +C
Sbjct: 8 PAPPKKTLQLYTPQYYGLC 26
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,406,130
Number of Sequences: 5004
Number of extensions: 48136
Number of successful extensions: 172
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 149
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 170
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 264253462
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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