Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0690
(623 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 154 1e-38
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 154 1e-38
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 154 1e-38
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 48 1e-06
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 48 1e-06
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 44 2e-05
SPCC364.03 |rpl1702|rpl17-2, rpl17|60S ribosomal protein L17|Sch... 29 0.41
SPCC1494.10 ||SPCC70.01|transcription factor |Schizosaccharomyce... 28 0.95
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 28 0.95
SPAC23D3.08 |usp108||U1 snRNP-associated protein Usp108|Schizosa... 27 2.2
SPBC2F12.04 |rpl1701|rpl17, rpl17-1|60S ribosomal protein L17|Sc... 27 2.2
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 27 2.9
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 26 5.1
SPBC18H10.20c |||conserved fungal protein|Schizosaccharomyces po... 25 6.7
SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificit... 25 6.7
SPAC637.05c |vma2||V-type ATPase V1 subunit B |Schizosaccharomyc... 25 6.7
SPCC1753.01c |ssb2|SPCC584.06c, rpa2|single-stranded DNA binding... 25 8.9
SPAC1952.11c |ure2||urease |Schizosaccharomyces pombe|chr 1|||Ma... 25 8.9
SPBC11B10.07c |||CDC50 domain protein|Schizosaccharomyces pombe|... 25 8.9
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 154 bits (373), Expect = 1e-38
Identities = 69/93 (74%), Positives = 77/93 (82%)
Frame = +2
Query: 257 PANXTTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFT 436
PA TTEVKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FT
Sbjct: 280 PAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFT 339
Query: 437 AQVIVLNHPGQISNGYTPVLDCHTPTLPANFAE 535
AQVI+LNHPGQIS GY+PVLDCHT + FAE
Sbjct: 340 AQVIILNHPGQISAGYSPVLDCHTAHIACKFAE 372
Score = 140 bits (339), Expect = 1e-34
Identities = 61/80 (76%), Positives = 69/80 (86%)
Frame = +3
Query: 6 GDNMLEPSTKMPWFKGWQVERKEGKADGKCLIEALDAILPPARPTDKPLRLPXQDVYKIG 185
GDNM+EP+T MPW++GWQ E K G GK L+EA+D+I PPARPTDKPLRLP QDVYKIG
Sbjct: 196 GDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPARPTDKPLRLPLQDVYKIG 255
Query: 186 GIGTVPVGRVETGVLKPGTI 245
GIGTVPVGRVETGV+KPG I
Sbjct: 256 GIGTVPVGRVETGVIKPGMI 275
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 154 bits (373), Expect = 1e-38
Identities = 69/93 (74%), Positives = 77/93 (82%)
Frame = +2
Query: 257 PANXTTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFT 436
PA TTEVKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FT
Sbjct: 280 PAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFT 339
Query: 437 AQVIVLNHPGQISNGYTPVLDCHTPTLPANFAE 535
AQVI+LNHPGQIS GY+PVLDCHT + FAE
Sbjct: 340 AQVIILNHPGQISAGYSPVLDCHTAHIACKFAE 372
Score = 140 bits (339), Expect = 1e-34
Identities = 61/80 (76%), Positives = 69/80 (86%)
Frame = +3
Query: 6 GDNMLEPSTKMPWFKGWQVERKEGKADGKCLIEALDAILPPARPTDKPLRLPXQDVYKIG 185
GDNM+EP+T MPW++GWQ E K G GK L+EA+D+I PPARPTDKPLRLP QDVYKIG
Sbjct: 196 GDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPARPTDKPLRLPLQDVYKIG 255
Query: 186 GIGTVPVGRVETGVLKPGTI 245
GIGTVPVGRVETGV+KPG I
Sbjct: 256 GIGTVPVGRVETGVIKPGMI 275
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 154 bits (373), Expect = 1e-38
Identities = 69/93 (74%), Positives = 77/93 (82%)
Frame = +2
Query: 257 PANXTTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFT 436
PA TTEVKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FT
Sbjct: 280 PAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFT 339
Query: 437 AQVIVLNHPGQISNGYTPVLDCHTPTLPANFAE 535
AQVI+LNHPGQIS GY+PVLDCHT + FAE
Sbjct: 340 AQVIILNHPGQISAGYSPVLDCHTAHIACKFAE 372
Score = 140 bits (339), Expect = 1e-34
Identities = 61/80 (76%), Positives = 69/80 (86%)
Frame = +3
Query: 6 GDNMLEPSTKMPWFKGWQVERKEGKADGKCLIEALDAILPPARPTDKPLRLPXQDVYKIG 185
GDNM+EP+T MPW++GWQ E K G GK L+EA+D+I PPARPTDKPLRLP QDVYKIG
Sbjct: 196 GDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPARPTDKPLRLPLQDVYKIG 255
Query: 186 GIGTVPVGRVETGVLKPGTI 245
GIGTVPVGRVETGV+KPG I
Sbjct: 256 GIGTVPVGRVETGVIKPGMI 275
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 48.0 bits (109), Expect = 1e-06
Identities = 24/49 (48%), Positives = 31/49 (63%), Gaps = 1/49 (2%)
Frame = +3
Query: 96 LIEALDAILP-PARPTDKPLRLPXQDVYKIGGIGTVPVGRVETGVLKPG 239
L+EA+D+ + P R TD P + +DV+ I G GTV GRVE G LK G
Sbjct: 234 LMEAVDSYITLPERKTDVPFLMAIEDVFSISGRGTVVTGRVERGTLKKG 282
Score = 33.9 bits (74), Expect = 0.019
Identities = 14/39 (35%), Positives = 24/39 (61%)
Frame = +2
Query: 272 TEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYV 388
T V +EM + L AV GDN G ++++ ++L+RG +
Sbjct: 296 TTVTGIEMFKKQLDAAVAGDNCGLLLRSIKREQLKRGMI 334
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 47.6 bits (108), Expect = 1e-06
Identities = 28/94 (29%), Positives = 47/94 (50%), Gaps = 1/94 (1%)
Frame = +2
Query: 257 PANXTTEVKSV-EMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADF 433
P N T EV ++ + E + ++ GD V V+ +++ GYV +KN P F
Sbjct: 503 PINQTLEVTAIYDEADEEISSSICGDQVRLRVRGDD-SDVQTGYVLTSTKN-PVHATTRF 560
Query: 434 TAQVIVLNHPGQISNGYTPVLDCHTPTLPANFAE 535
AQ+ +L P ++ GY+ V+ HT +FA+
Sbjct: 561 IAQIAILELPSILTTGYSCVMHIHTAVEEVSFAK 594
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 44.0 bits (99), Expect = 2e-05
Identities = 23/46 (50%), Positives = 28/46 (60%)
Frame = +3
Query: 87 GKCLIEALDAILPPARPTDKPLRLPXQDVYKIGGIGTVPVGRVETG 224
G L+ ALD ++PP +P KPLRL DVY+ TV GRVE G
Sbjct: 384 GPTLLSALDQLVPPEKPYRKPLRLSIDDVYRSPRSVTV-TGRVEAG 428
>SPCC364.03 |rpl1702|rpl17-2, rpl17|60S ribosomal protein
L17|Schizosaccharomyces pombe|chr 3|||Manual
Length = 187
Score = 29.5 bits (63), Expect = 0.41
Identities = 12/24 (50%), Positives = 16/24 (66%)
Frame = -1
Query: 587 GLTSVRLPSTTVNFFLDFLQNLQA 516
G+T R P +VNFF D L+N +A
Sbjct: 77 GVTQARWPVKSVNFFYDLLKNAEA 100
>SPCC1494.10 ||SPCC70.01|transcription factor |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 964
Score = 28.3 bits (60), Expect = 0.95
Identities = 16/50 (32%), Positives = 23/50 (46%)
Frame = +2
Query: 413 PKGAADFTAQVIVLNHPGQISNGYTPVLDCHTPTLPANFAENPRKS*PSY 562
P + +A + NHP Q SN + P + T+P N+ N PSY
Sbjct: 265 PHNSFPASADRLQKNHPVQSSN-FNPYTPAPSITVPPNYIPNTAMMGPSY 313
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 28.3 bits (60), Expect = 0.95
Identities = 20/68 (29%), Positives = 29/68 (42%)
Frame = +3
Query: 36 MPWFKGWQVERKEGKADGKCLIEALDAILPPARPTDKPLRLPXQDVYKIGGIGTVPVGRV 215
+P K V K GK + L E + I PP + PLR D + G + + R+
Sbjct: 206 IPMSKPILVSSKTGKNVEQILPEIIQKIPPPKGSENAPLRCLLIDSWYNSYQGVIGLVRI 265
Query: 216 ETGVLKPG 239
G +K G
Sbjct: 266 MEGFIKKG 273
>SPAC23D3.08 |usp108||U1 snRNP-associated protein
Usp108|Schizosaccharomyces pombe|chr 1|||Manual
Length = 382
Score = 27.1 bits (57), Expect = 2.2
Identities = 23/76 (30%), Positives = 33/76 (43%), Gaps = 3/76 (3%)
Frame = -2
Query: 376 TQFLDGHVLYVET---YIVSRYSFLESFVVHLHRLDFSSXVGGGKTTMVPGFNTPVSTLP 206
T +D V+ +ET YI+SR SF+ V + D + + G +T + P
Sbjct: 162 TNEIDASVV-IETLLKYILSRISFIPRVSVVPYIPDAAYGIYGSPSTFTQFNHMSSMVTP 220
Query: 205 TGTVPIPPILYTSXWG 158
IPPI TS G
Sbjct: 221 ENPYGIPPIALTSAGG 236
>SPBC2F12.04 |rpl1701|rpl17, rpl17-1|60S ribosomal protein
L17|Schizosaccharomyces pombe|chr 2|||Manual
Length = 187
Score = 27.1 bits (57), Expect = 2.2
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = -1
Query: 587 GLTSVRLPSTTVNFFLDFLQNLQA 516
G+T R P +V FF D L+N +A
Sbjct: 77 GVTQARWPVKSVKFFYDLLKNAEA 100
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 26.6 bits (56), Expect = 2.9
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = -2
Query: 373 QFLDGHVLYVETYIVSRYSFLESFV 299
+F DGH+L ETY+ LES V
Sbjct: 527 KFFDGHLLASETYVGGHVESLESGV 551
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 25.8 bits (54), Expect = 5.1
Identities = 13/47 (27%), Positives = 22/47 (46%)
Frame = +3
Query: 411 HLRVLQILQLKSLCLTILVKSQTVTHQSWIATLPHCLQILQKIQEKV 551
HL + +Q S I+ S T ++W T HC++I+ Q +
Sbjct: 346 HLASVNSVQYSSKTGLIVTASSDRTLRTWDITTGHCIRIIHAHQRGI 392
>SPBC18H10.20c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 361
Score = 25.4 bits (53), Expect = 6.7
Identities = 12/37 (32%), Positives = 17/37 (45%)
Frame = -3
Query: 336 TLSPGTASWRASWCISTDLTSVVXLAGXRQQWYLVST 226
T PGT W SW L + V + ++YL +T
Sbjct: 101 TYRPGTQHWPFSWLFPGSLPASVSNRYIKLEYYLEAT 137
>SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificity
factor complex subunit Rna14|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 733
Score = 25.4 bits (53), Expect = 6.7
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = -2
Query: 229 NTPVSTLPTGTVPIPPIL 176
N P S LPT VP+P I+
Sbjct: 666 NPPTSALPTVPVPLPSII 683
>SPAC637.05c |vma2||V-type ATPase V1 subunit B |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 503
Score = 25.4 bits (53), Expect = 6.7
Identities = 8/20 (40%), Positives = 15/20 (75%)
Frame = +1
Query: 481 LHTSLGLPHSHIACKFCRKS 540
+ ++ GLPH+ IA + CR++
Sbjct: 168 IFSAAGLPHNEIAAQICRQA 187
>SPCC1753.01c |ssb2|SPCC584.06c, rpa2|single-stranded DNA binding
protein Ssb2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 279
Score = 25.0 bits (52), Expect = 8.9
Identities = 12/33 (36%), Positives = 17/33 (51%)
Frame = -2
Query: 322 YSFLESFVVHLHRLDFSSXVGGGKTTMVPGFNT 224
+ FLE+ VHLH ++ V G PG+ T
Sbjct: 158 FHFLEAIAVHLHFTQKANAVNGAN---APGYGT 187
>SPAC1952.11c |ure2||urease |Schizosaccharomyces pombe|chr
1|||Manual
Length = 835
Score = 25.0 bits (52), Expect = 8.9
Identities = 14/45 (31%), Positives = 18/45 (40%)
Frame = -2
Query: 136 GRAGGRMASRASMRHFPSALPSLRSTCHPLNQGILVEGSNMLSPC 2
G GG S+ P+ LPS + P L E +ML C
Sbjct: 545 GAGGGHAPDIISLVQNPNILPSSTNPTRPFTTNTLDEELDMLMVC 589
>SPBC11B10.07c |||CDC50 domain protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 371
Score = 25.0 bits (52), Expect = 8.9
Identities = 15/42 (35%), Positives = 22/42 (52%)
Frame = -1
Query: 593 NXGLTSVRLPSTTVNFFLDFLQNLQAMWECGNPRLVCNRLRF 468
N G ++PST + F ++N+ AMW+ VC LRF
Sbjct: 77 NIGDEFKQVPSTNIEFQYKNVKNVTAMWKSSGD--VCT-LRF 115
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,837,038
Number of Sequences: 5004
Number of extensions: 60837
Number of successful extensions: 203
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 194
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 203
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 275671126
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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