Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= e96h0609
         (823 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces ...    55   1e-08
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po...    44   2e-05
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ...    43   6e-05
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po...    42   1e-04
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ...    34   0.028
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha...    29   0.80 
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c...    29   0.80 
SPBC1773.09c |mug184||meiotically upregulated gene Mug184|Schizo...    28   1.4  
SPAC27D7.03c |mei2||RNA-binding protein involved in meiosis Mei2...    27   2.4  
SPBC409.10 |ade7||phosphoribosylamidoimidazolesuccinocarboxamide...    27   3.2  
SPBC3B8.03 |||saccharopine dehydrogenase |Schizosaccharomyces po...    27   4.3  
SPAC4A8.05c |myp2|myo3|myosin II heavy chain |Schizosaccharomyce...    27   4.3  
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc...    25   9.8  

>SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 632

 Score = 54.8 bits (126), Expect = 1e-08
 Identities = 25/78 (32%), Positives = 46/78 (58%)
 Frame = +1

Query: 262 VKVAKVDCTRFTAVASHFHIRAYPTILFLKGSFQHEYTGDRVKEDLINYAMRMSQPAVQR 441
           +K+A+V+C     + +HFHI+ +PT    +G    +Y G    + L++Y+ +++     +
Sbjct: 230 LKMAQVNCDEEKEMCNHFHIKKFPTFRVFQGFDSIQYNGPLKYQQLLSYSNQVASYQAIK 289

Query: 442 ISRGNSIEYLKESHPVFF 495
           I  G+ IE ++ SHPVFF
Sbjct: 290 IEEGD-IESIENSHPVFF 306


>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 492

 Score = 44.4 bits (100), Expect = 2e-05
 Identities = 21/74 (28%), Positives = 40/74 (54%), Gaps = 1/74 (1%)
 Frame = +1

Query: 262 VKVAKVDCTRFTAVASHFHIRAYPTI-LFLKGSFQHEYTGDRVKEDLINYAMRMSQPAVQ 438
           + + +VDCT    + S + IR YPT+ +F  G    +Y+G R  + L+ Y  +   P V+
Sbjct: 73  ISLVEVDCTEEGDLCSEYSIRGYPTLNVFKNGKQISQYSGPRKHDALVKYMRKQLLPTVK 132

Query: 439 RISRGNSIEYLKES 480
            IS+     +++++
Sbjct: 133 PISKDTLENFVEKA 146



 Score = 39.9 bits (89), Expect = 4e-04
 Identities = 15/36 (41%), Positives = 25/36 (69%)
 Frame = +2

Query: 137 DRFLEFSKDGMWFVKFYAPWCSHCRRMEPTWAHVAQ 244
           D  ++ +KD +  V+FYAPWC HC+ + PT+  +A+
Sbjct: 367 DIVMDETKDVL--VEFYAPWCGHCKNLAPTYEKLAE 400



 Score = 39.5 bits (88), Expect = 6e-04
 Identities = 14/30 (46%), Positives = 18/30 (60%)
 Frame = +2

Query: 161 DGMWFVKFYAPWCSHCRRMEPTWAHVAQAL 250
           D +  VKFYAPWC HC+ + P +   A  L
Sbjct: 39  DKVLMVKFYAPWCGHCKALAPEYESAADEL 68


>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 726

 Score = 42.7 bits (96), Expect = 6e-05
 Identities = 19/55 (34%), Positives = 30/55 (54%)
 Frame = +2

Query: 80  IIFFTRIFNVSSSRVLELSDRFLEFSKDGMWFVKFYAPWCSHCRRMEPTWAHVAQ 244
           + F T +  + S   L  +D   E SK G WF+K+Y P C  C+R+ P W ++ +
Sbjct: 16  VAFITMVQTLVSGVPLTDNDLESEVSK-GTWFIKYYLPSCGACKRLGPMWDNMVE 69



 Score = 33.5 bits (73), Expect = 0.037
 Identities = 19/88 (21%), Positives = 37/88 (42%)
 Frame = +1

Query: 262 VKVAKVDCTRFTAVASHFHIRAYPTILFLKGSFQHEYTGDRVKEDLINYAMRMSQPAVQR 441
           + VA ++C         + I+ +PT LF K     EY G   + DL+++A   +   ++ 
Sbjct: 331 LNVAHINCAVSKRACKQYSIQYFPTFLFFKEEAFVEYVGLPNEGDLVSFAEEAANFEIRE 390

Query: 442 ISRGNSIEYLKESHPVFFGFIGNRKGFY 525
           +   +++   K     F  F  +    Y
Sbjct: 391 VELLDTVNAEKNGDVFFLYFYDDDSAEY 418



 Score = 27.9 bits (59), Expect = 1.8
 Identities = 10/31 (32%), Positives = 17/31 (54%)
 Frame = +2

Query: 158 KDGMWFVKFYAPWCSHCRRMEPTWAHVAQAL 250
           K+G WF++FY+  C  C  +   W  +A  +
Sbjct: 298 KEG-WFIQFYSSECDDCDDVSTAWYAMANRM 327


>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 363

 Score = 41.9 bits (94), Expect = 1e-04
 Identities = 20/50 (40%), Positives = 31/50 (62%), Gaps = 2/50 (4%)
 Frame = +2

Query: 116 SRVLELSDR-FLEFSK-DGMWFVKFYAPWCSHCRRMEPTWAHVAQALYIL 259
           S  +EL+ + F +F K  G   V FYAPWC +C+++ PT+  +A  L+ L
Sbjct: 31  SNTIELNSKNFRKFVKAKGPSLVVFYAPWCGYCKKLVPTYQKLASNLHSL 80


>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 359

 Score = 33.9 bits (74), Expect = 0.028
 Identities = 18/57 (31%), Positives = 28/57 (49%), Gaps = 5/57 (8%)
 Frame = +2

Query: 74  LYIIFFTRIFNVSSSRVLELS-----DRFLEFSKDGMWFVKFYAPWCSHCRRMEPTW 229
           L  + F     V +S V+EL      +  +  SK G   ++FYA WC HC+ + P +
Sbjct: 6   LSFVIFALFALVFASGVVELQSLNELENTIRASKKGA-LIEFYATWCGHCKSLAPVY 61



 Score = 33.9 bits (74), Expect = 0.028
 Identities = 10/18 (55%), Positives = 15/18 (83%)
 Frame = +2

Query: 176 VKFYAPWCSHCRRMEPTW 229
           V+FYA WC +C+R+ PT+
Sbjct: 163 VEFYADWCGYCKRLAPTY 180



 Score = 29.1 bits (62), Expect = 0.80
 Identities = 14/54 (25%), Positives = 27/54 (50%), Gaps = 2/54 (3%)
 Frame = +1

Query: 253 YTPVKVAKVDCTRFTAVASHFHIRAYPTILFL--KGSFQHEYTGDRVKEDLINY 408
           +  V + K+D    + VA  +HI  +PT+++    GS   +Y+  R  + L  +
Sbjct: 71  HNDVLIGKIDADTHSDVADKYHITGFPTLIWFPPDGSEPVQYSNARDVDSLTQF 124


>SPBC12D12.07c |trx2||mitochondrial thioredoxin
           Trx2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 121

 Score = 29.1 bits (62), Expect = 0.80
 Identities = 12/34 (35%), Positives = 16/34 (47%)
 Frame = +2

Query: 122 VLELSDRFLEFSKDGMWFVKFYAPWCSHCRRMEP 223
           V    D     S D +  V FYA WC  C+ ++P
Sbjct: 22  VESFGDYNTRISADKVTVVDFYADWCGPCKYLKP 55


>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 244

 Score = 29.1 bits (62), Expect = 0.80
 Identities = 11/40 (27%), Positives = 21/40 (52%)
 Frame = +2

Query: 125 LELSDRFLEFSKDGMWFVKFYAPWCSHCRRMEPTWAHVAQ 244
           +E     L+  K+ +  + FYAPW + C++M   +   A+
Sbjct: 8   VEQFQEILQNGKEQIILLNFYAPWAAPCKQMNQVFDQFAK 47


>SPBC1773.09c |mug184||meiotically upregulated gene
           Mug184|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 551

 Score = 28.3 bits (60), Expect = 1.4
 Identities = 11/46 (23%), Positives = 25/46 (54%)
 Frame = +2

Query: 125 LELSDRFLEFSKDGMWFVKFYAPWCSHCRRMEPTWAHVAQALYILP 262
           ++ + R  E+    M F K Y+ +C+  +    +W ++ + L+I+P
Sbjct: 459 IDFNVRNREYWNQLMVFQKLYSKYCTESQHFINSWINIKKELHIVP 504


>SPAC27D7.03c |mei2||RNA-binding protein involved in meiosis
           Mei2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 750

 Score = 27.5 bits (58), Expect = 2.4
 Identities = 11/37 (29%), Positives = 20/37 (54%)
 Frame = +1

Query: 412 MRMSQPAVQRISRGNSIEYLKESHPVFFGFIGNRKGF 522
           +R  Q +  + S  NSI+YL ++  +F  F+ N   +
Sbjct: 62  IRNIQLSATKSSESNSIDYLTDTQNIFPNFVNNENNY 98


>SPBC409.10 |ade7||phosphoribosylamidoimidazolesuccinocarboxamide
           synthase Ade7|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 299

 Score = 27.1 bits (57), Expect = 3.2
 Identities = 11/34 (32%), Positives = 17/34 (50%)
 Frame = +2

Query: 707 KQHLRQWITSERFGFFPKIPEPILIVXLVTGKYI 808
           KQ+LR W+T+      P +  P  +V     KY+
Sbjct: 253 KQYLRNWLTANNLANKPNVSLPEDVVEATRRKYV 286


>SPBC3B8.03 |||saccharopine dehydrogenase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 450

 Score = 26.6 bits (56), Expect = 4.3
 Identities = 10/17 (58%), Positives = 11/17 (64%)
 Frame = -1

Query: 772 WLGYFGEKPKSLRSNPL 722
           WLG F  KP + R NPL
Sbjct: 323 WLGMFSSKPVTPRGNPL 339


>SPAC4A8.05c |myp2|myo3|myosin II heavy chain |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 2104

 Score = 26.6 bits (56), Expect = 4.3
 Identities = 12/34 (35%), Positives = 19/34 (55%)
 Frame = +1

Query: 361 QHEYTGDRVKEDLINYAMRMSQPAVQRISRGNSI 462
           Q EYT +R++ D I+Y   + QP +  I +   I
Sbjct: 495 QEEYTQERIEWDFIDYGNDL-QPTIDAIEKSEPI 527


>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 103

 Score = 25.4 bits (53), Expect = 9.8
 Identities = 8/22 (36%), Positives = 13/22 (59%)
 Frame = +2

Query: 158 KDGMWFVKFYAPWCSHCRRMEP 223
           +D +  V F+A WC  C+ + P
Sbjct: 17  QDKLVVVDFFATWCGPCKAIAP 38


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,487,322
Number of Sequences: 5004
Number of extensions: 74657
Number of successful extensions: 182
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 169
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 181
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 402440190
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)