BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e96h0580 (538 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g03490.1 68416.m00347 peroxisomal protein PEX19 family protei... 39 0.002 At5g17550.1 68418.m02059 peroxisomal protein PEX19 family protei... 38 0.004 At3g60300.1 68416.m06740 RWD domain-containing protein contains ... 32 0.28 At5g66670.1 68418.m08404 hypothetical protein contains Pfam:PF05... 29 1.5 At5g50390.1 68418.m06241 pentatricopeptide (PPR) repeat-containi... 29 2.6 At2g18630.1 68415.m02169 expressed protein unusual splice site a... 29 2.6 At3g28320.1 68416.m03538 hypothetical protein similar to At14a (... 27 6.0 >At3g03490.1 68416.m00347 peroxisomal protein PEX19 family protein contains Pfam profile: PF04614 Pex19 protein family Length = 248 Score = 39.1 bits (87), Expect = 0.002 Identities = 14/25 (56%), Positives = 22/25 (88%) Frame = +1 Query: 49 KVLELMQKMQDLGQPPTELVGDIGP 123 K++E+MQKMQ+ GQPP+++V +I P Sbjct: 200 KIMEIMQKMQECGQPPSDIVKEIDP 224 >At5g17550.1 68418.m02059 peroxisomal protein PEX19 family protein contains Pfam profile: PF04614 Pex19 protein family Length = 245 Score = 37.9 bits (84), Expect = 0.004 Identities = 13/25 (52%), Positives = 22/25 (88%) Frame = +1 Query: 49 KVLELMQKMQDLGQPPTELVGDIGP 123 K++E+MQKMQ+ GQPP+++V ++ P Sbjct: 197 KIMEIMQKMQECGQPPSDIVQEMDP 221 >At3g60300.1 68416.m06740 RWD domain-containing protein contains weak similarity to RING finger protein 25 (RING finger protein AO7) (Swiss-Prot:Q9QZR0) [Mus musculus] Length = 366 Score = 31.9 bits (69), Expect = 0.28 Identities = 13/29 (44%), Positives = 20/29 (68%) Frame = +1 Query: 7 LDSDSEEVKRKRFEKVLELMQKMQDLGQP 93 L SDSE ++R+RFE +L+ ++ L QP Sbjct: 239 LQSDSENIRRERFESILKTQEEKGGLVQP 267 >At5g66670.1 68418.m08404 hypothetical protein contains Pfam:PF05055: Protein of unknown function (DUF677) Length = 408 Score = 29.5 bits (63), Expect = 1.5 Identities = 12/29 (41%), Positives = 19/29 (65%) Frame = +1 Query: 7 LDSDSEEVKRKRFEKVLELMQKMQDLGQP 93 +D+D E K+K++ K LE M K + +G P Sbjct: 165 VDTDLGESKKKKYVKTLEEMNKFKAMGDP 193 >At5g50390.1 68418.m06241 pentatricopeptide (PPR) repeat-containing protein contains INTERPRO:IPR002885 PPR repeats Length = 701 Score = 28.7 bits (61), Expect = 2.6 Identities = 13/34 (38%), Positives = 22/34 (64%) Frame = +1 Query: 1 NELDSDSEEVKRKRFEKVLELMQKMQDLGQPPTE 102 + DS +E VKR+ ++KV ELM+++ + G E Sbjct: 581 DRFDSYNETVKRQIYQKVDELMEEISEYGYSEEE 614 >At2g18630.1 68415.m02169 expressed protein unusual splice site at second intron; GA instead of conserved GT at donor site; similar to At14a GI:11994571 and GI:11994573 [Arabidopsis thaliana] Length = 393 Score = 28.7 bits (61), Expect = 2.6 Identities = 9/34 (26%), Positives = 24/34 (70%) Frame = +1 Query: 1 NELDSDSEEVKRKRFEKVLELMQKMQDLGQPPTE 102 N+ + ++E+ + +++EK LE +++ + G+P T+ Sbjct: 150 NQFEEENEDKENRKYEKTLEELKRFKVAGEPFTK 183 >At3g28320.1 68416.m03538 hypothetical protein similar to At14a (GI:11994571 and GI:11994573) [Arabidopsis thaliana] Length = 280 Score = 27.5 bits (58), Expect = 6.0 Identities = 14/34 (41%), Positives = 20/34 (58%), Gaps = 4/34 (11%) Frame = +1 Query: 4 ELDSDSEE----VKRKRFEKVLELMQKMQDLGQP 93 + DSEE VK+KR+ K LE + K + +G P Sbjct: 30 QFKKDSEEKDVGVKKKRYGKTLEELMKFKAMGNP 63 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,664,122 Number of Sequences: 28952 Number of extensions: 235960 Number of successful extensions: 679 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 638 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 679 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 993966856 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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