Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0562
(606 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S... 104 4e-23
At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) str... 104 4e-23
At2g43680.2 68415.m05430 calmodulin-binding family protein simil... 33 0.19
At2g43680.1 68415.m05429 calmodulin-binding family protein simil... 33 0.19
At5g05340.1 68418.m00575 peroxidase, putative similar to peroxid... 27 9.6
>At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S
roibosomal protein L4, Arabidopsis thaliana,
EMBL:CAA79104
Length = 407
Score = 104 bits (250), Expect = 4e-23
Identities = 47/85 (55%), Positives = 53/85 (62%)
Frame = +3
Query: 249 PWGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHXXXXXXXXXXXXXXXXXXTXVXXLVQA 428
P GGTHR+GQ AFGNMCRGGRMFAPTK WRRWH T V LV A
Sbjct: 86 PGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWHRRVNVNMKRHAIVSAIAATAVPALVMA 145
Query: 429 XGHIIEKIPELPLVVADKVQEINKT 503
GH IE +PE+PLVV+D + + KT
Sbjct: 146 RGHKIENVPEMPLVVSDSAEAVEKT 170
Score = 82.6 bits (195), Expect = 2e-16
Identities = 41/71 (57%), Positives = 48/71 (67%), Gaps = 2/71 (2%)
Frame = +1
Query: 13 SVARPLVSVYSEKSE--TVQGAAKPLPFVFKAPIRPXLVNDVHVSMSKNSRQPYCVSKEA 186
+ ARPLV+V + T Q LP V AP+RP +VN VH +S NSRQPY VSK+A
Sbjct: 5 AAARPLVTVQGLDGDMSTDQSTTVTLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVSKKA 64
Query: 187 GHQTSAESWGT 219
GHQTSAESWGT
Sbjct: 65 GHQTSAESWGT 75
Score = 27.9 bits (59), Expect = 5.5
Identities = 13/37 (35%), Positives = 21/37 (56%)
Frame = +2
Query: 494 QQDQIAVIFLRRLKAWSDILKVYKSQXLRAXKGKMRN 604
++ A+ L+++ A+ D K S +R KGKMRN
Sbjct: 168 EKTSAAIKVLKQIGAYDDAEKAKNSIGIRPGKGKMRN 204
>At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) strong
similarity to 60S ribosomal protein L1 GB:P49691
Length = 406
Score = 104 bits (250), Expect = 4e-23
Identities = 47/85 (55%), Positives = 53/85 (62%)
Frame = +3
Query: 249 PWGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHXXXXXXXXXXXXXXXXXXTXVXXLVQA 428
P GGTHR+GQ AFGNMCRGGRMFAPTK WRRWH T V LV A
Sbjct: 85 PGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWHRRVNVNMKRHAIVSAIAATAVPALVMA 144
Query: 429 XGHIIEKIPELPLVVADKVQEINKT 503
GH IE +PE+PLVV+D + + KT
Sbjct: 145 RGHKIENVPEMPLVVSDSAEAVEKT 169
Score = 84.6 bits (200), Expect = 5e-17
Identities = 41/74 (55%), Positives = 52/74 (70%), Gaps = 2/74 (2%)
Frame = +1
Query: 4 MSLSVARPLVSVYSEKSE--TVQGAAKPLPFVFKAPIRPXLVNDVHVSMSKNSRQPYCVS 177
M+ + ARPLV++ + + T Q + LP V AP+RP +VN VH +S NSRQPY VS
Sbjct: 1 MAAAAARPLVTIQTLDGDMSTDQSSTVVLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVS 60
Query: 178 KEAGHQTSAESWGT 219
K+AGHQTSAESWGT
Sbjct: 61 KKAGHQTSAESWGT 74
Score = 27.9 bits (59), Expect = 5.5
Identities = 13/37 (35%), Positives = 21/37 (56%)
Frame = +2
Query: 494 QQDQIAVIFLRRLKAWSDILKVYKSQXLRAXKGKMRN 604
++ A+ L+++ A+ D K S +R KGKMRN
Sbjct: 167 EKTSAAIKVLKQIGAYDDAEKAKNSIGIRPGKGKMRN 203
>At2g43680.2 68415.m05430 calmodulin-binding family protein similar
to SF16 protein [Helianthus annuus] GI:560150; contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 669
Score = 32.7 bits (71), Expect = 0.19
Identities = 19/61 (31%), Positives = 26/61 (42%)
Frame = +2
Query: 302 WWTYVRXXEALAALAPSRQPPTAESGLGSSRCCYXRPXARSGXRTHY*KDSRASLGCSRQ 481
WW +V LA+ APS P + L SR C P ++S + H D+ R
Sbjct: 465 WWNWVDRQNPLASPAPSYSQPQRDFRLTPSRLC-PSPLSQSSKQHHIRLDNHFDTSTPRS 523
Query: 482 S 484
S
Sbjct: 524 S 524
>At2g43680.1 68415.m05429 calmodulin-binding family protein similar
to SF16 protein [Helianthus annuus] GI:560150; contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 668
Score = 32.7 bits (71), Expect = 0.19
Identities = 19/61 (31%), Positives = 26/61 (42%)
Frame = +2
Query: 302 WWTYVRXXEALAALAPSRQPPTAESGLGSSRCCYXRPXARSGXRTHY*KDSRASLGCSRQ 481
WW +V LA+ APS P + L SR C P ++S + H D+ R
Sbjct: 464 WWNWVDRQNPLASPAPSYSQPQRDFRLTPSRLC-PSPLSQSSKQHHIRLDNHFDTSTPRS 522
Query: 482 S 484
S
Sbjct: 523 S 523
>At5g05340.1 68418.m00575 peroxidase, putative similar to peroxidase
[Nicotiana tabacum] gi|5381253|dbj|BAA82306; similar to
Peroxidase P7 [Brassica rapa (Turnip)] SWISS-PROT:P00434
Length = 324
Score = 27.1 bits (57), Expect = 9.6
Identities = 15/46 (32%), Positives = 21/46 (45%)
Frame = +3
Query: 3 NESIGSPTFSVGVFREE*DGAGCSQAPPVRIQGAHTSGPGQ*CSRF 140
N +I +PT S+ G S V + GAHT G + C+ F
Sbjct: 163 NSNIPAPTSSLSQLISSFSAVGLSTRDMVALSGAHTIGQSR-CTNF 207
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,405,994
Number of Sequences: 28952
Number of extensions: 209981
Number of successful extensions: 572
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 550
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 570
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1206913392
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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