Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0533
(726 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g19770.1 68415.m02310 profilin 4 (PRO4) (PFN4) identical to p... 50 2e-06
At4g29340.1 68417.m04192 profilin 3 (PRO3) (PFN3) identical to p... 49 4e-06
At5g56600.1 68418.m07065 profilin 5 (PRO5) (PRF3) identical to S... 48 5e-06
At4g29350.1 68417.m04193 profilin 2 (PRO2) (PFN2) (PRF2) identic... 48 5e-06
At2g19760.1 68415.m02309 profilin 1 (PRO1) (PFN1) (PRF1) / aller... 48 8e-06
At2g41560.1 68415.m05136 calcium-transporting ATPase 4, plasma m... 35 0.063
At1g27770.2 68414.m03396 calcium-transporting ATPase 1, plasma m... 34 0.083
At1g27770.1 68414.m03395 calcium-transporting ATPase 1, plasma m... 34 0.083
At3g57330.1 68416.m06381 calcium-transporting ATPase, plasma mem... 29 2.4
At2g22950.1 68415.m02725 calcium-transporting ATPase, plasma mem... 28 5.5
At1g12940.1 68414.m01503 high-affinity nitrate transporter, puta... 28 5.5
At5g08450.2 68418.m00996 expressed protein KED, Nicotiana tabacu... 27 9.6
At5g08450.1 68418.m00995 expressed protein KED, Nicotiana tabacu... 27 9.6
At4g39660.1 68417.m05608 alanine--glyoxylate aminotransferase, p... 27 9.6
At4g37390.1 68417.m05294 auxin-responsive GH3 family protein sim... 27 9.6
At4g18010.2 68417.m02680 inositol polyphosphate 5-phosphatase II... 27 9.6
At4g18010.1 68417.m02679 inositol polyphosphate 5-phosphatase II... 27 9.6
>At2g19770.1 68415.m02310 profilin 4 (PRO4) (PFN4) identical to
profilin 4 SP:Q38905 GI:1353768 from [Arabidopsis
thaliana]
Length = 134
Score = 50.0 bits (114), Expect = 2e-06
Identities = 28/71 (39%), Positives = 38/71 (53%), Gaps = 7/71 (9%)
Frame = +3
Query: 63 MSWQDYVDKQLMAS------RCVTKAAIAGHDGNVWAKSEGF-EISKDEVAKIVAGFENE 221
MSWQ YVD+ LM +T AAI GHDG+VWA+S F + E+ I+ F+
Sbjct: 1 MSWQAYVDEHLMCDVGDGQGHHLTAAAIIGHDGSVWAQSANFPQFKPQEITDIMKDFDEP 60
Query: 222 SLLTSGGVTIA 254
L G+ +A
Sbjct: 61 GHLAPTGMFLA 71
Score = 46.8 bits (106), Expect = 1e-05
Identities = 22/43 (51%), Positives = 28/43 (65%)
Frame = +1
Query: 307 GKVGVHCMKTQQAVVIXLYEEPIQPQQAASVVEKLGEYLITCG 435
G G+ KT Q++V LYEEP+ P Q VVE+LG+YLI G
Sbjct: 91 GAGGITIKKTGQSMVFGLYEEPVTPGQCNMVVERLGDYLIEQG 133
>At4g29340.1 68417.m04192 profilin 3 (PRO3) (PFN3) identical to
profilin 3 SP:Q38904 GI:1353765 from [Arabidopsis
thaliana]
Length = 134
Score = 48.8 bits (111), Expect = 4e-06
Identities = 28/71 (39%), Positives = 38/71 (53%), Gaps = 7/71 (9%)
Frame = +3
Query: 63 MSWQDYVDKQLMAS------RCVTKAAIAGHDGNVWAKSEGF-EISKDEVAKIVAGFENE 221
MSWQ YVD+ LM +T AAI GHDG+VWA+S F + E + I+ F+
Sbjct: 1 MSWQTYVDEHLMCDVGDGQGHHLTAAAIVGHDGSVWAQSANFPQFKGQEFSDIMKDFDEP 60
Query: 222 SLLTSGGVTIA 254
L G+ +A
Sbjct: 61 GHLAPTGLFMA 71
Score = 44.4 bits (100), Expect = 8e-05
Identities = 20/43 (46%), Positives = 27/43 (62%)
Frame = +1
Query: 307 GKVGVHCMKTQQAVVIXLYEEPIQPQQAASVVEKLGEYLITCG 435
G G+ KT Q+ V +YEEP+ P Q VVE+LG+YL+ G
Sbjct: 91 GAGGITIKKTGQSCVFGIYEEPVTPGQCNMVVERLGDYLLEQG 133
>At5g56600.1 68418.m07065 profilin 5 (PRO5) (PRF3) identical to
SP|Q9FE63 Profilin 5 {Arabidopsis thaliana}
Length = 168
Score = 48.4 bits (110), Expect = 5e-06
Identities = 27/65 (41%), Positives = 35/65 (53%), Gaps = 4/65 (6%)
Frame = +3
Query: 63 MSWQDYVDKQLM---ASRCVTKAAIAGHDGNVWAKSEGF-EISKDEVAKIVAGFENESLL 230
MSWQ YVD LM A +T AAI G DG+VWA+S F ++ +E+ I F L
Sbjct: 38 MSWQTYVDDHLMCDVAGNRLTAAAILGQDGSVWAQSNNFPQVKPEEIQGIKDDFTTPGTL 97
Query: 231 TSGGV 245
G+
Sbjct: 98 APTGL 102
Score = 44.4 bits (100), Expect = 8e-05
Identities = 22/43 (51%), Positives = 26/43 (60%)
Frame = +1
Query: 307 GKVGVHCMKTQQAVVIXLYEEPIQPQQAASVVEKLGEYLITCG 435
G GV KT A+V +Y+EP+ P Q VVE LGEYLI G
Sbjct: 125 GAGGVTIKKTTLALVFGIYDEPMTPGQCNMVVENLGEYLIESG 167
>At4g29350.1 68417.m04193 profilin 2 (PRO2) (PFN2) (PRF2) identical
to profilin 2 SP:Q42418 GI:1353772 from [Arabidopsis
thaliana]; identical to cDNA profilin (PRF2) GI:9965570
Length = 131
Score = 48.4 bits (110), Expect = 5e-06
Identities = 28/65 (43%), Positives = 35/65 (53%), Gaps = 4/65 (6%)
Frame = +3
Query: 63 MSWQDYVDKQLMAS---RCVTKAAIAGHDGNVWAKSEGF-EISKDEVAKIVAGFENESLL 230
MSWQ YVD LM +T AAI G DG+VWA+S F ++ E+A I FE L
Sbjct: 1 MSWQSYVDDHLMCEVEGNHLTHAAIFGQDGSVWAQSSAFPQLKPAEIAGINKDFEEAGHL 60
Query: 231 TSGGV 245
G+
Sbjct: 61 APTGL 65
Score = 42.7 bits (96), Expect = 2e-04
Identities = 21/43 (48%), Positives = 27/43 (62%)
Frame = +1
Query: 307 GKVGVHCMKTQQAVVIXLYEEPIQPQQAASVVEKLGEYLITCG 435
G GV KT QA+V +Y+EP+ Q VVE+LG+YLI G
Sbjct: 88 GPGGVTIKKTTQALVFGIYDEPMTGGQCNLVVERLGDYLIESG 130
>At2g19760.1 68415.m02309 profilin 1 (PRO1) (PFN1) (PRF1) / allergen
Ara t 8 identical to profilin 1 (Allergen Ara t 8)
SP:Q42449 GI:1353770 from [Arabidopsis thaliana]
Length = 131
Score = 47.6 bits (108), Expect = 8e-06
Identities = 27/65 (41%), Positives = 34/65 (52%), Gaps = 4/65 (6%)
Frame = +3
Query: 63 MSWQDYVDKQLMAS---RCVTKAAIAGHDGNVWAKSEGF-EISKDEVAKIVAGFENESLL 230
MSWQ YVD LM +T AAI G DG+VWA+S F ++ E+ I FE L
Sbjct: 1 MSWQSYVDDHLMCDVEGNHLTAAAILGQDGSVWAQSAKFPQLKPQEIDGIKKDFEEPGFL 60
Query: 231 TSGGV 245
G+
Sbjct: 61 APTGL 65
Score = 40.7 bits (91), Expect = 0.001
Identities = 20/40 (50%), Positives = 25/40 (62%)
Frame = +1
Query: 307 GKVGVHCMKTQQAVVIXLYEEPIQPQQAASVVEKLGEYLI 426
G GV KT QA+V Y+EP+ Q VVE+LG+YLI
Sbjct: 88 GPGGVTIKKTNQALVFGFYDEPMTGGQCNLVVERLGDYLI 127
>At2g41560.1 68415.m05136 calcium-transporting ATPase 4, plasma
membrane-type / Ca2+-ATPase, isoform 4 (ACA4) identical
to SP|O22218 Calcium-transporting ATPase 4, plasma
membrane-type (EC 3.6.3.8) (Ca(2+)-ATPase isoform 4)
{Arabidopsis thaliana}
Length = 1030
Score = 34.7 bits (76), Expect = 0.063
Identities = 18/48 (37%), Positives = 27/48 (56%)
Frame = +3
Query: 156 KSEGFEISKDEVAKIVAGFENESLLTSGGVTIAARGTSTSVAQTISSA 299
K GF I DE+A +V + +SL GGV A+ S S+++ I S+
Sbjct: 89 KKAGFSIEADELASMVRKNDTKSLAQKGGVEELAKKVSVSLSEGIRSS 136
>At1g27770.2 68414.m03396 calcium-transporting ATPase 1, plasma
membrane-type / Ca(2+)-ATPase isoform 1 (ACA1) / plastid
envelope ATPase 1 (PEA1) identical to SP|Q37145
Calcium-transporting ATPase 1, plasma membrane-type (EC
3.6.3.8) (Ca(2+)-ATPase isoform 1) (Plastid envelope
ATPase 1) {Arabidopsis thaliana}; identical to cDNA
envelope Ca2+-ATPase (PEA1) chloroplast gene encoding
chloroplast protein GI:509809
Length = 946
Score = 34.3 bits (75), Expect = 0.083
Identities = 18/48 (37%), Positives = 25/48 (52%)
Frame = +3
Query: 156 KSEGFEISKDEVAKIVAGFENESLLTSGGVTIAARGTSTSVAQTISSA 299
+ GFEI DE+ IV G + + L GG STS+A IS++
Sbjct: 92 RKAGFEICPDELGSIVEGHDLKKLKIHGGTEGLTEKLSTSIASGISTS 139
>At1g27770.1 68414.m03395 calcium-transporting ATPase 1, plasma
membrane-type / Ca(2+)-ATPase isoform 1 (ACA1) / plastid
envelope ATPase 1 (PEA1) identical to SP|Q37145
Calcium-transporting ATPase 1, plasma membrane-type (EC
3.6.3.8) (Ca(2+)-ATPase isoform 1) (Plastid envelope
ATPase 1) {Arabidopsis thaliana}; identical to cDNA
envelope Ca2+-ATPase (PEA1) chloroplast gene encoding
chloroplast protein GI:509809
Length = 1020
Score = 34.3 bits (75), Expect = 0.083
Identities = 18/48 (37%), Positives = 25/48 (52%)
Frame = +3
Query: 156 KSEGFEISKDEVAKIVAGFENESLLTSGGVTIAARGTSTSVAQTISSA 299
+ GFEI DE+ IV G + + L GG STS+A IS++
Sbjct: 92 RKAGFEICPDELGSIVEGHDLKKLKIHGGTEGLTEKLSTSIASGISTS 139
>At3g57330.1 68416.m06381 calcium-transporting ATPase, plasma
membrane-type, putative / Ca2+-ATPase, putative (ACA11)
identical to SP|Q9M2L4|ACAB_ARATH Potential
calcium-transporting ATPase 11, plasma membrane-type (EC
3.6.3.8) (Ca(2+)-ATPase isoform 11) {Arabidopsis
thaliana}; strong similarity to calmodulin-stimulated
calcium-ATPase [Brassica oleracea] GI:1805654
Length = 1025
Score = 29.5 bits (63), Expect = 2.4
Identities = 17/40 (42%), Positives = 20/40 (50%)
Frame = -1
Query: 393 CGLLRLDGFFIEXNDHSLLCLHAMHADLAKFARMIWSVPL 274
CG+L G IE +D L H M A L K M S+PL
Sbjct: 686 CGILTAGGVAIEGSDFRNLPPHEMRAILPKIQVMARSLPL 725
>At2g22950.1 68415.m02725 calcium-transporting ATPase, plasma
membrane-type, putative / Ca2+-ATPase, putative (ACA7)
identical to SP|O64806 Potential calcium-transporting
ATPase 7, plasma membrane-type (EC 3.6.3.8)
(Ca(2+)-ATPase isoform 7) {Arabidopsis thaliana}; strong
similarity to SP|O81108 Calcium-transporting ATPase 2,
plasma membrane-type (EC 3.6.3.8) (Ca(2+)-ATPase isoform
2) {Arabidopsis thaliana}
Length = 1015
Score = 28.3 bits (60), Expect = 5.5
Identities = 13/30 (43%), Positives = 18/30 (60%)
Frame = +3
Query: 156 KSEGFEISKDEVAKIVAGFENESLLTSGGV 245
K+ GF+I DE+ IV G + + L GGV
Sbjct: 90 KAAGFDICADELGSIVEGHDVKKLKFHGGV 119
>At1g12940.1 68414.m01503 high-affinity nitrate transporter,
putative similar to trans-membrane nitrate transporter
protein AtNRT2:1 [Arabidopsis thaliana] GI:3747058,
high-affinity nitrate transporter ACH1 [Arabidopsis
thaliana] GI:3608362
Length = 502
Score = 28.3 bits (60), Expect = 5.5
Identities = 18/54 (33%), Positives = 27/54 (50%), Gaps = 1/54 (1%)
Frame = +3
Query: 138 DGNVWAKSEGFEISKDEVAKIVA-GFENESLLTSGGVTIAARGTSTSVAQTISS 296
DG+ WA + E KD+V K+++ G +N G +T A G V TI +
Sbjct: 233 DGDYWAMHKSGEREKDDVGKVISNGIKN----YRGWITALAYGYCFGVELTIDN 282
>At5g08450.2 68418.m00996 expressed protein KED, Nicotiana tabacum,
EMBL:AB009883
Length = 918
Score = 27.5 bits (58), Expect = 9.6
Identities = 12/30 (40%), Positives = 17/30 (56%)
Frame = -1
Query: 519 IQNALLISPWKKYYLIVLLDHGELRPLITT 430
IQ L PW KY + ++ D G +PL T+
Sbjct: 754 IQYNLCNEPWIKYSISIVADKGLKKPLFTS 783
>At5g08450.1 68418.m00995 expressed protein KED, Nicotiana tabacum,
EMBL:AB009883
Length = 918
Score = 27.5 bits (58), Expect = 9.6
Identities = 12/30 (40%), Positives = 17/30 (56%)
Frame = -1
Query: 519 IQNALLISPWKKYYLIVLLDHGELRPLITT 430
IQ L PW KY + ++ D G +PL T+
Sbjct: 754 IQYNLCNEPWIKYSISIVADKGLKKPLFTS 783
>At4g39660.1 68417.m05608 alanine--glyoxylate aminotransferase,
putative / beta-alanine-pyruvate aminotransferase,
putative / AGT, putative similar to SP|Q64565
Alanine--glyoxylate aminotransferase 2, mitochondrial
precursor (EC 2.6.1.44) (AGT 2) (Beta-alanine-pyruvate
aminotransferase) {Rattus norvegicus}; contains Pfam
profile PF00202: aminotransferase, class III
Length = 476
Score = 27.5 bits (58), Expect = 9.6
Identities = 19/58 (32%), Positives = 28/58 (48%)
Frame = +3
Query: 126 IAGHDGNVWAKSEGFEISKDEVAKIVAGFENESLLTSGGVTIAARGTSTSVAQTISSA 299
+ G DG+++AK I K VAGF E++ GG A G SV + + +A
Sbjct: 227 VFGSDGSLYAKDVHDHIEYGTSGK-VAGFIAETIQGVGGAVELAPGYLKSVYEIVRNA 283
>At4g37390.1 68417.m05294 auxin-responsive GH3 family protein
similar to auxin-responsive GH3 product [Glycine max]
GI:18591; contains Pfam profile PF03321: GH3
auxin-responsive promoter
Length = 603
Score = 27.5 bits (58), Expect = 9.6
Identities = 19/66 (28%), Positives = 31/66 (46%)
Frame = +3
Query: 39 YF*LNLIKMSWQDYVDKQLMASRCVTKAAIAGHDGNVWAKSEGFEISKDEVAKIVAGFEN 218
YF +NL M V +M + + HDG+ A++ E S E+A + G E
Sbjct: 344 YFGINLKPMCKPSEVSYTIMPNMAYFEFLPHNHDGDGAAEASLDETSLVELANVEVGKEY 403
Query: 219 ESLLTS 236
E ++T+
Sbjct: 404 ELVITT 409
>At4g18010.2 68417.m02680 inositol polyphosphate 5-phosphatase II
(IP5PII) nearly identical to inositol polyphosphate
5-phosphatase II [Arabidopsis thaliana] GI:10444263
isoform contains an AT-acceptor splice site at intron 6
Length = 613
Score = 27.5 bits (58), Expect = 9.6
Identities = 22/99 (22%), Positives = 42/99 (42%), Gaps = 2/99 (2%)
Frame = +3
Query: 174 ISKDEVAKI-VAGFENESLLTSGGVTIAA-RGTSTSVAQTISSARTWQGRRALHEDTASC 347
+S D + I + GF+ L +G V A RG I + ++++ + SC
Sbjct: 135 LSTDNPSDIYIIGFQEVVPLNAGNVFGAEDRGPIPKWESIIRRTLNKSNKESVYDQSPSC 194
Query: 348 GHXSL*RTHPTSAGRICRGEVRRIFNYLWLLEVEARRDL 464
+ +L R+H + I E I +++ + + A L
Sbjct: 195 NNNALHRSHSAPSSPILAQEANSIISHVMVENLVADHSL 233
>At4g18010.1 68417.m02679 inositol polyphosphate 5-phosphatase II
(IP5PII) nearly identical to inositol polyphosphate
5-phosphatase II [Arabidopsis thaliana] GI:10444263
isoform contains an AT-acceptor splice site at intron 6
Length = 646
Score = 27.5 bits (58), Expect = 9.6
Identities = 22/99 (22%), Positives = 42/99 (42%), Gaps = 2/99 (2%)
Frame = +3
Query: 174 ISKDEVAKI-VAGFENESLLTSGGVTIAA-RGTSTSVAQTISSARTWQGRRALHEDTASC 347
+S D + I + GF+ L +G V A RG I + ++++ + SC
Sbjct: 135 LSTDNPSDIYIIGFQEVVPLNAGNVFGAEDRGPIPKWESIIRRTLNKSNKESVYDQSPSC 194
Query: 348 GHXSL*RTHPTSAGRICRGEVRRIFNYLWLLEVEARRDL 464
+ +L R+H + I E I +++ + + A L
Sbjct: 195 NNNALHRSHSAPSSPILAQEANSIISHVMVENLVADHSL 233
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,758,819
Number of Sequences: 28952
Number of extensions: 322816
Number of successful extensions: 688
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 672
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 683
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1584903024
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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