Bombyx BLAST Result against uniref50

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= e96h0518
         (439 letters)

Database: uniref50 
           1,657,284 sequences; 575,637,011 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_P17980 Cluster: 26S protease regulatory subunit 6A; n=1...   101   7e-21
UniRef50_UPI0000E467F1 Cluster: PREDICTED: similar to 26S protea...    70   3e-11
UniRef50_Q89C84 Cluster: HlyD family secretion protein; n=14; Al...    33   2.0  
UniRef50_UPI0000E45C15 Cluster: PREDICTED: similar to ankyrin 2,...    32   6.2  
UniRef50_Q4RJ16 Cluster: Chromosome 1 SCAF15039, whole genome sh...    32   6.2  
UniRef50_P22373 Cluster: Probable DNA polymerase; n=1; Claviceps...    31   8.1  

>UniRef50_P17980 Cluster: 26S protease regulatory subunit 6A; n=154;
           Eukaryota|Rep: 26S protease regulatory subunit 6A - Homo
           sapiens (Human)
          Length = 439

 Score =  101 bits (242), Expect = 7e-21
 Identities = 48/61 (78%), Positives = 52/61 (85%)
 Frame = +3

Query: 255 IXXNTEXIKVXKTLPYLVSXVXELXDVDPQEEEEDGAVVDLDSQRKGKCAVIKTSTRQTY 434
           I  N+E IKV KTLPYLVS V EL DVDP ++EEDGA +DLDSQRKGKCAVIKTSTRQTY
Sbjct: 73  IKENSEKIKVNKTLPYLVSNVIELLDVDPNDQEEDGANIDLDSQRKGKCAVIKTSTRQTY 132

Query: 435 F 437
           F
Sbjct: 133 F 133



 Score = 69.7 bits (163), Expect = 3e-11
 Identities = 34/68 (50%), Positives = 49/68 (72%), Gaps = 3/68 (4%)
 Frame = +1

Query: 64  NITMATTLEDK--SIWEDGEE-ALSEEVLRMPTDEIVSRTRLLXNXIKIMXSEVMRXSHE 234
           NI    T ++K  ++W++ E+  + EEVL+M T+EI+ RTRLL + IKIM SEV+R +HE
Sbjct: 6   NIESPVTRQEKMATVWDEAEQDGIGEEVLKMSTEEIIQRTRLLDSEIKIMKSEVLRVTHE 65

Query: 235 LQAXNDKL 258
           LQA  DK+
Sbjct: 66  LQAMKDKI 73


>UniRef50_UPI0000E467F1 Cluster: PREDICTED: similar to 26S protease
           regulatory subunit 6a, partial; n=1; Strongylocentrotus
           purpuratus|Rep: PREDICTED: similar to 26S protease
           regulatory subunit 6a, partial - Strongylocentrotus
           purpuratus
          Length = 79

 Score = 69.7 bits (163), Expect = 3e-11
 Identities = 30/43 (69%), Positives = 37/43 (86%)
 Frame = +1

Query: 76  ATTLEDKSIWEDGEEALSEEVLRMPTDEIVSRTRLLXNXIKIM 204
           AT LEDKSIWEDGE+++ EEVLRM TDEI+ RTRLL N +K++
Sbjct: 3   ATNLEDKSIWEDGEDSVGEEVLRMSTDEIIGRTRLLDNEVKLL 45



 Score = 59.3 bits (137), Expect = 4e-08
 Identities = 30/43 (69%), Positives = 34/43 (79%), Gaps = 1/43 (2%)
 Frame = +3

Query: 303 LVSXVXELXDVDPQEEEE-DGAVVDLDSQRKGKCAVIKTSTRQ 428
           L+    +L DV P E+EE +GA VDLDSQRKGKCAVIKTSTRQ
Sbjct: 37  LLDNEVKLLDVQPDEDEETEGANVDLDSQRKGKCAVIKTSTRQ 79


>UniRef50_Q89C84 Cluster: HlyD family secretion protein; n=14;
           Alphaproteobacteria|Rep: HlyD family secretion protein -
           Bradyrhizobium japonicum
          Length = 456

 Score = 33.5 bits (73), Expect = 2.0
 Identities = 27/80 (33%), Positives = 36/80 (45%)
 Frame = +1

Query: 19  QELLNLKDYYEKTNHNITMATTLEDKSIWEDGEEALSEEVLRMPTDEIVSRTRLLXNXIK 198
           QEL  ++D YEK    I+  TTLE  S   +GE A           +I      +    K
Sbjct: 213 QELTGVRDLYEKHLVQISRLTTLERDSARLNGERAQYIASRAQAKGKITETELQIIQVDK 272

Query: 199 IMXSEVMRXSHELQAXNDKL 258
            M SEV   S +L+  NDK+
Sbjct: 273 DMVSEV---SKDLRETNDKI 289


>UniRef50_UPI0000E45C15 Cluster: PREDICTED: similar to ankyrin
            2,3/unc44; n=4; Strongylocentrotus purpuratus|Rep:
            PREDICTED: similar to ankyrin 2,3/unc44 -
            Strongylocentrotus purpuratus
          Length = 1846

 Score = 31.9 bits (69), Expect = 6.2
 Identities = 13/27 (48%), Positives = 22/27 (81%), Gaps = 1/27 (3%)
 Frame = +1

Query: 16   VQELLNLKDYYEKTNHNITMATT-LED 93
            ++++L+LKD Y K +HNIT++ T +ED
Sbjct: 1113 IEKVLSLKDVYGKMSHNITLSVTPIED 1139


>UniRef50_Q4RJ16 Cluster: Chromosome 1 SCAF15039, whole genome
           shotgun sequence; n=1; Tetraodon nigroviridis|Rep:
           Chromosome 1 SCAF15039, whole genome shotgun sequence -
           Tetraodon nigroviridis (Green puffer)
          Length = 270

 Score = 31.9 bits (69), Expect = 6.2
 Identities = 25/82 (30%), Positives = 37/82 (45%), Gaps = 2/82 (2%)
 Frame = +1

Query: 19  QELLNLKDYYEKTNHNITMATTLEDKSIWEDGEEALSEE--VLRMPTDEIVSRTRLLXNX 192
           QE L LK+ Y K    +     LE     E  +E  S E  +L    +E+ +  R L   
Sbjct: 71  QERLKLKEEYSKLETELK--AVLEKNRDHECAQERFSSEKTLLAKAKEELEAEKRELVRT 128

Query: 193 IKIMXSEVMRXSHELQAXNDKL 258
           ++   SEV   + +L+  NDKL
Sbjct: 129 LERRSSEVEHLNDDLKHLNDKL 150


>UniRef50_P22373 Cluster: Probable DNA polymerase; n=1; Claviceps
            purpurea|Rep: Probable DNA polymerase - Claviceps
            purpurea (Ergot fungus) (Sphacelia purpurea)
          Length = 1063

 Score = 31.5 bits (68), Expect = 8.1
 Identities = 16/65 (24%), Positives = 33/65 (50%)
 Frame = +1

Query: 28   LNLKDYYEKTNHNITMATTLEDKSIWEDGEEALSEEVLRMPTDEIVSRTRLLXNXIKIMX 207
            L L+DY +  N  +  AT    K  ++DG   +S+  +++       R+R++    K + 
Sbjct: 978  LTLEDYEKMYNKEVVEATKTSSKRNYKDGFVTISDSTVKLNPTSYTKRSRVISKD-KWIG 1036

Query: 208  SEVMR 222
            +E++R
Sbjct: 1037 TEIIR 1041


  Database: uniref50
    Posted date:  Oct 5, 2007 11:19 AM
  Number of letters in database: 575,637,011
  Number of sequences in database:  1,657,284
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 280,752,759
Number of Sequences: 1657284
Number of extensions: 3330924
Number of successful extensions: 9663
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 9510
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 9659
length of database: 575,637,011
effective HSP length: 93
effective length of database: 421,509,599
effective search space used: 21918499148
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)