Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0481
(747 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) riboso... 165 2e-41
At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B) 165 3e-41
At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contai... 165 3e-41
At4g10710.1 68417.m01751 transcriptional regulator-related simil... 31 0.81
At1g29760.1 68414.m03639 expressed protein 31 0.81
At3g11400.1 68416.m01390 eukaryotic translation initiation facto... 29 3.3
At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase, ... 29 3.3
At5g25270.1 68418.m02996 expressed protein 29 4.3
At4g13100.2 68417.m02042 zinc finger (C3HC4-type RING finger) fa... 29 4.3
At4g05360.1 68417.m00818 zinc knuckle (CCHC-type) family protein... 29 4.3
At4g08180.3 68417.m01353 oxysterol-binding family protein simila... 27 10.0
At4g08180.2 68417.m01352 oxysterol-binding family protein simila... 27 10.0
At4g08180.1 68417.m01351 oxysterol-binding family protein simila... 27 10.0
>At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) ribosomal
protein S4, Arabidopsis thaliana, PIR:T48480
Length = 262
Score = 165 bits (402), Expect = 2e-41
Identities = 84/162 (51%), Positives = 102/162 (62%)
Frame = +3
Query: 255 MDXVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHDGRT 434
MD VSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + G K +PYL T+DGRT
Sbjct: 87 MDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQKGIPYLNTYDGRT 146
Query: 435 IRYPDPLIKVNDSIQLDIATTKIMDSSSLSPGTCV*SREAVTWGAWAPSCPARDIPAPST 614
IRYPDPLIK ND+I+LD+ KI++ G V G
Sbjct: 147 IRYPDPLIKPNDTIKLDLEANKIVEFIKFDVGNVVMVTGGRNRGRVGVIKNREKHKGSFE 206
Query: 615 LCTSRTPTGHTFATRLNNVFIIGKGTKAYISLPRGKGIRLTI 740
+ TGH FATRL NV+ IGKGTK ++SLP+GKGI+LTI
Sbjct: 207 TIHIQDSTGHEFATRLGNVYTIGKGTKPWVSLPKGKGIKLTI 248
Score = 143 bits (347), Expect = 1e-34
Identities = 65/84 (77%), Positives = 73/84 (86%)
Frame = +1
Query: 1 ARGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEV 180
ARG KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV
Sbjct: 2 ARGLKKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREV 61
Query: 181 LKIVKQRLIKVDGKVRTDPTYPAG 252
+ I+ QR I+VDGKVRTD TYPAG
Sbjct: 62 ISILMQRHIQVDGKVRTDKTYPAG 85
Score = 69.3 bits (162), Expect = 3e-12
Identities = 32/57 (56%), Positives = 41/57 (71%)
Frame = +2
Query: 509 FIKFESGNLCMITGGRNLGRVGTIVSRERHPGSFDIVHIKDSNGTHLRHEVEQRVHN 679
FIKF+ GN+ M+TGGRN GRVG I +RE+H GSF+ +HI+DS G HE R+ N
Sbjct: 172 FIKFDVGNVVMVTGGRNRGRVGVIKNREKHKGSFETIHIQDSTG----HEFATRLGN 224
>At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B)
Length = 262
Score = 165 bits (401), Expect = 3e-41
Identities = 84/162 (51%), Positives = 102/162 (62%)
Frame = +3
Query: 255 MDXVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHDGRT 434
MD VSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + G K +PYL T+DGRT
Sbjct: 87 MDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQKGIPYLNTYDGRT 146
Query: 435 IRYPDPLIKVNDSIQLDIATTKIMDSSSLSPGTCV*SREAVTWGAWAPSCPARDIPAPST 614
IRYPDPLIK ND+I+LD+ KI++ G V G
Sbjct: 147 IRYPDPLIKPNDTIKLDLEENKIVEFIKFDVGNVVMVTGGRNRGRVGVIKNREKHKGSFE 206
Query: 615 LCTSRTPTGHTFATRLNNVFIIGKGTKAYISLPRGKGIRLTI 740
+ TGH FATRL NV+ IGKGTK ++SLP+GKGI+LTI
Sbjct: 207 TIHIQDSTGHEFATRLGNVYTIGKGTKPWVSLPKGKGIKLTI 248
Score = 143 bits (347), Expect = 1e-34
Identities = 65/84 (77%), Positives = 73/84 (86%)
Frame = +1
Query: 1 ARGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEV 180
ARG KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV
Sbjct: 2 ARGLKKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREV 61
Query: 181 LKIVKQRLIKVDGKVRTDPTYPAG 252
+ I+ QR I+VDGKVRTD TYPAG
Sbjct: 62 ISILMQRHIQVDGKVRTDKTYPAG 85
Score = 69.3 bits (162), Expect = 3e-12
Identities = 32/57 (56%), Positives = 41/57 (71%)
Frame = +2
Query: 509 FIKFESGNLCMITGGRNLGRVGTIVSRERHPGSFDIVHIKDSNGTHLRHEVEQRVHN 679
FIKF+ GN+ M+TGGRN GRVG I +RE+H GSF+ +HI+DS G HE R+ N
Sbjct: 172 FIKFDVGNVVMVTGGRNRGRVGVIKNREKHKGSFETIHIQDSTG----HEFATRLGN 224
>At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contains
ribosomal protein S4 signature from residues 8 to 22
Length = 261
Score = 165 bits (401), Expect = 3e-41
Identities = 84/162 (51%), Positives = 102/162 (62%)
Frame = +3
Query: 255 MDXVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHDGRT 434
MD VSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + G K +PYL T+DGRT
Sbjct: 87 MDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQKGIPYLNTYDGRT 146
Query: 435 IRYPDPLIKVNDSIQLDIATTKIMDSSSLSPGTCV*SREAVTWGAWAPSCPARDIPAPST 614
IRYPDPLIK ND+I+LD+ KI++ G V G
Sbjct: 147 IRYPDPLIKPNDTIKLDLEENKIVEFIKFDVGNVVMVTGGRNRGRVGVIKNREKHKGSFE 206
Query: 615 LCTSRTPTGHTFATRLNNVFIIGKGTKAYISLPRGKGIRLTI 740
+ TGH FATRL NV+ IGKGTK ++SLP+GKGI+LTI
Sbjct: 207 TIHIQDSTGHEFATRLGNVYTIGKGTKPWVSLPKGKGIKLTI 248
Score = 143 bits (347), Expect = 1e-34
Identities = 65/84 (77%), Positives = 73/84 (86%)
Frame = +1
Query: 1 ARGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEV 180
ARG KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV
Sbjct: 2 ARGLKKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREV 61
Query: 181 LKIVKQRLIKVDGKVRTDPTYPAG 252
+ I+ QR I+VDGKVRTD TYPAG
Sbjct: 62 ISILMQRHIQVDGKVRTDKTYPAG 85
Score = 69.3 bits (162), Expect = 3e-12
Identities = 32/57 (56%), Positives = 41/57 (71%)
Frame = +2
Query: 509 FIKFESGNLCMITGGRNLGRVGTIVSRERHPGSFDIVHIKDSNGTHLRHEVEQRVHN 679
FIKF+ GN+ M+TGGRN GRVG I +RE+H GSF+ +HI+DS G HE R+ N
Sbjct: 172 FIKFDVGNVVMVTGGRNRGRVGVIKNREKHKGSFETIHIQDSTG----HEFATRLGN 224
>At4g10710.1 68417.m01751 transcriptional regulator-related similar
to chromatin-specific transcription elongation factor
FACT 140 kDa subunit (GI:5499741) [Homo sapiens]
Length = 1074
Score = 31.1 bits (67), Expect = 0.81
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = -2
Query: 122 KHSRNLWGPVDGLGAYTPPSLSNIHAL 42
KHS +LWG D L TPP+ ++ L
Sbjct: 44 KHSADLWGSADALAIATPPASDDLRYL 70
>At1g29760.1 68414.m03639 expressed protein
Length = 526
Score = 31.1 bits (67), Expect = 0.81
Identities = 14/31 (45%), Positives = 17/31 (54%)
Frame = -2
Query: 233 SVLTFPSTFMRRCFTIFRTSFPVKAYFRRFL 141
S+LTFP +R CF F F + RRFL
Sbjct: 195 SLLTFPPWLLRNCFLFFFDPFSTIRFGRRFL 225
>At3g11400.1 68416.m01390 eukaryotic translation initiation factor
3G / eIF3g nearly identical to eukaryotic translation
initiation factor 3g [Arabidopsis thaliana] GI:12407751
Length = 294
Score = 29.1 bits (62), Expect = 3.3
Identities = 9/26 (34%), Positives = 15/26 (57%)
Frame = +3
Query: 570 WAPSCPARDIPAPSTLCTSRTPTGHT 647
W CP +D+ AP+ + + PTG +
Sbjct: 149 WTSKCPYKDLAAPTDVFIDKPPTGES 174
>At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase,
putative / steroid 5-alpha-reductase, putative identical
to gi:1280611; contains a steroid 5-alpha reductase,
C-terminal domain
Length = 262
Score = 29.1 bits (62), Expect = 3.3
Identities = 15/39 (38%), Positives = 19/39 (48%)
Frame = -2
Query: 212 TFMRRCFTIFRTSFPVKAYFRRFLRKITRGKHSRNLWGP 96
TF R C + P A +FL+ GKH+R WGP
Sbjct: 8 TFFRYCLLTLIFAGPPTAVLLKFLQA-PYGKHNRTGWGP 45
>At5g25270.1 68418.m02996 expressed protein
Length = 540
Score = 28.7 bits (61), Expect = 4.3
Identities = 22/56 (39%), Positives = 25/56 (44%), Gaps = 2/56 (3%)
Frame = -3
Query: 667 LFNLVAKVCPVGVLDVHNVEGAGMSLAGHDGAHAPQVTASRDHTQV--PGLKLDES 506
L N VA V PV V + G S G DG H P +SR V PG + D S
Sbjct: 349 LRNPVALVIPV-VARYQQISLGGRSSTGLDGVHQPVTESSRQPQSVGTPGREGDSS 403
>At4g13100.2 68417.m02042 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 265
Score = 28.7 bits (61), Expect = 4.3
Identities = 19/58 (32%), Positives = 29/58 (50%), Gaps = 5/58 (8%)
Frame = +2
Query: 566 RVGTIVSRERHPGSFDIVHIKDSNGTHL-----RHEVEQRVHNRQGHEGVHLAAARQG 724
R+GT+++ ER P ++ + + N H+ R EVE V R G +G A A G
Sbjct: 156 RLGTVLAAEREPPRMSLMELLEDNDGHMYELSARDEVE--VEGRDGCDGGGEAVAVTG 211
>At4g05360.1 68417.m00818 zinc knuckle (CCHC-type) family protein
contains Pfam domain, PF00098: Zinc knuckle
Length = 735
Score = 28.7 bits (61), Expect = 4.3
Identities = 11/27 (40%), Positives = 18/27 (66%)
Frame = +1
Query: 562 GARGHHRVPRETSRLLRHCAHQGLQRD 642
G +GH + R R +R C+H+GL+R+
Sbjct: 668 GVQGH--IKRNCFRFIRECSHEGLRRN 692
>At4g08180.3 68417.m01353 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 813
Score = 27.5 bits (58), Expect = 10.0
Identities = 12/33 (36%), Positives = 20/33 (60%)
Frame = +1
Query: 145 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY 243
N ++A+T NE+ +K+RL D ++R D Y
Sbjct: 703 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRY 735
>At4g08180.2 68417.m01352 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 813
Score = 27.5 bits (58), Expect = 10.0
Identities = 12/33 (36%), Positives = 20/33 (60%)
Frame = +1
Query: 145 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY 243
N ++A+T NE+ +K+RL D ++R D Y
Sbjct: 703 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRY 735
>At4g08180.1 68417.m01351 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 814
Score = 27.5 bits (58), Expect = 10.0
Identities = 12/33 (36%), Positives = 20/33 (60%)
Frame = +1
Query: 145 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY 243
N ++A+T NE+ +K+RL D ++R D Y
Sbjct: 704 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRY 736
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,051,214
Number of Sequences: 28952
Number of extensions: 408736
Number of successful extensions: 1164
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 1113
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1164
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1653386488
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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