Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0472
(798 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 68 1e-12
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 62 7e-11
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 57 3e-09
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 42 1e-04
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 40 4e-04
SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr ... 35 0.016
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 31 0.25
SPAC22E12.16c |pik1||phosphatidylinositol kinase Pik1|Schizosacc... 27 2.4
SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces... 27 3.1
SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyce... 27 3.1
SPAC1565.07c |||TATA binding protein interacting protein |Schizo... 27 3.1
SPAC23C11.15 |pst2||Clr6 histone deacetylase complex subunit Pst... 26 5.4
SPBC16D10.06 |||ZIP zinc transporter 2|Schizosaccharomyces pombe... 26 5.4
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 68.1 bits (159), Expect = 1e-12
Identities = 28/62 (45%), Positives = 46/62 (74%)
Frame = +3
Query: 303 DFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQGNINYENFVHL 482
+F++G + FDKD G I ELR++L++LGEKLS++E+++LL+G G +NY +FV +
Sbjct: 78 EFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGVPVKDGMVNYHDFVQM 137
Query: 483 IM 488
I+
Sbjct: 138 IL 139
Score = 46.4 bits (105), Expect = 5e-06
Identities = 27/62 (43%), Positives = 36/62 (58%), Gaps = 1/62 (1%)
Frame = +2
Query: 89 FQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERISFEVFRQ-FT 265
+++AF LFD G G+I IGD LRA GQNPT +++ + L P E + E F Q
Sbjct: 8 YKQAFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEITEIESTL-PAE-VDMEQFLQVLN 65
Query: 266 RP 271
RP
Sbjct: 66 RP 67
Score = 27.5 bits (58), Expect = 2.4
Identities = 12/38 (31%), Positives = 21/38 (55%)
Frame = +2
Query: 86 EFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDV 199
EF + FQ+FD G I V ++ L +LG+ + ++
Sbjct: 78 EFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEM 115
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 62.5 bits (145), Expect = 7e-11
Identities = 29/66 (43%), Positives = 42/66 (63%), Gaps = 1/66 (1%)
Frame = +3
Query: 291 DTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLL-QGQEDSQGNINYE 467
D + E + FDKDGNG+I+ EL H+L++LGE+LS +EV ++ + D G INYE
Sbjct: 82 DNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDGDGVINYE 141
Query: 468 NFVHLI 485
F +I
Sbjct: 142 EFSRVI 147
Score = 48.8 bits (111), Expect = 9e-07
Identities = 20/45 (44%), Positives = 34/45 (75%)
Frame = +2
Query: 68 SEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVK 202
+++Q+AEF+EAF LFD DG I ++G +R+LGQ+PT ++++
Sbjct: 7 TDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQ 51
Score = 33.9 bits (74), Expect = 0.027
Identities = 17/62 (27%), Positives = 36/62 (58%), Gaps = 1/62 (1%)
Frame = +3
Query: 303 DFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLL-QGQEDSQGNINYENFVH 479
+F E FD+D +G I+S EL ++ +LG+ + E++ ++ + D G I++ F+
Sbjct: 13 EFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFLT 72
Query: 480 LI 485
++
Sbjct: 73 MM 74
Score = 33.5 bits (73), Expect = 0.036
Identities = 15/42 (35%), Positives = 26/42 (61%)
Frame = +2
Query: 74 DQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDV 199
D E +EAF++FD G+G I V ++ L +LG+ ++ +V
Sbjct: 82 DNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEV 123
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 57.2 bits (132), Expect = 3e-09
Identities = 26/67 (38%), Positives = 44/67 (65%), Gaps = 1/67 (1%)
Frame = +3
Query: 291 DTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQ-GQEDSQGNINYE 467
++ ++I+ R FDKD +G+I +A+ + TLGEKLSD+EV+ ++Q + G+ +Y
Sbjct: 75 ESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEADPTNSGSFDYY 134
Query: 468 NFVHLIM 488
+FV IM
Sbjct: 135 DFVQRIM 141
Score = 41.9 bits (94), Expect = 1e-04
Identities = 20/51 (39%), Positives = 32/51 (62%)
Frame = +2
Query: 68 SEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHL 220
S++Q E +EAF L+D DG I + +G LR+LG N T++++ K + L
Sbjct: 4 SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNEL 54
Score = 35.1 bits (77), Expect = 0.012
Identities = 15/56 (26%), Positives = 30/56 (53%)
Frame = +2
Query: 86 EFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERISFEVF 253
E+ +AF++FD G I A+ D ++ LG+ ++++V+ P SF+ +
Sbjct: 79 EYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEADPTNSGSFDYY 134
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 41.5 bits (93), Expect = 1e-04
Identities = 22/62 (35%), Positives = 33/62 (53%)
Frame = +3
Query: 300 NDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQGNINYENFVH 479
ND +E FD +G I + +R LS++G+++ EVE +L+ S G YE FV
Sbjct: 117 NDLLEAFSTFDDTQSGKIPISTMRDALSSMGDRMDPQEVESILRSY-TSHGVFYYEKFVD 175
Query: 480 LI 485
I
Sbjct: 176 AI 177
Score = 33.9 bits (74), Expect = 0.027
Identities = 17/56 (30%), Positives = 26/56 (46%)
Frame = +2
Query: 59 AGYSEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKP 226
A + Q+ E +EAF L D GDG I + L +L Q+ +E + + P
Sbjct: 40 AQLTSSQIQELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSINHMFESINP 95
Score = 29.9 bits (64), Expect = 0.44
Identities = 11/40 (27%), Positives = 23/40 (57%)
Frame = +3
Query: 312 EGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQ 431
E DKDG+G I +++ +L++L + S+D + + +
Sbjct: 52 EAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSINHMFE 91
Score = 29.5 bits (63), Expect = 0.58
Identities = 13/36 (36%), Positives = 20/36 (55%)
Frame = +2
Query: 95 EAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVK 202
EAF FD GKI ++ + DAL ++G +V+
Sbjct: 121 EAFSTFDDTQSGKIPISTMRDALSSMGDRMDPQEVE 156
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 39.9 bits (89), Expect = 4e-04
Identities = 20/46 (43%), Positives = 29/46 (63%)
Frame = +2
Query: 68 SEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKK 205
+E+Q + EAF+LFDS D I ++ A+RALG N +S+V K
Sbjct: 32 TEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLK 77
Score = 32.7 bits (71), Expect = 0.063
Identities = 21/77 (27%), Positives = 37/77 (48%), Gaps = 1/77 (1%)
Frame = +3
Query: 264 PGHIESTQGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQG-QE 440
P +E T+ + D E + FD D + I ELR + LG EV ++L+ +
Sbjct: 26 PLRVEITE-EQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDK 84
Query: 441 DSQGNINYENFVHLIMQ 491
+G + E+FV ++ +
Sbjct: 85 TGKGYLQMEDFVRVMTE 101
Score = 29.5 bits (63), Expect = 0.58
Identities = 13/43 (30%), Positives = 23/43 (53%)
Frame = +2
Query: 74 DQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVK 202
D L E + AF+LFD GKI + + + L +N + +++
Sbjct: 107 DPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELE 149
>SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 621
Score = 34.7 bits (76), Expect = 0.016
Identities = 15/57 (26%), Positives = 28/57 (49%)
Frame = +3
Query: 321 RHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQGNINYENFVHLIMQ 491
RHF+K + ++ E L++LG +E L +S+ + YE F ++M+
Sbjct: 494 RHFEKKKSNMLNEVEFYAALASLGLVYDTEEGTALFHRAANSEEGVTYERFTEIVME 550
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 30.7 bits (66), Expect = 0.25
Identities = 16/52 (30%), Positives = 27/52 (51%)
Frame = +3
Query: 327 FDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQGNINYENFVHL 482
FD G+I +LR + LGE L+ +++ QL+ + G ++ E F L
Sbjct: 21 FDVTHKGYIDFEDLRRSCAQLGENLTKEQL-QLMLDLAGTNGKVSREEFAEL 71
Score = 25.8 bits (54), Expect = 7.2
Identities = 13/39 (33%), Positives = 20/39 (51%)
Frame = +2
Query: 86 EFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVK 202
E +EAF LFD G I + + LG+N T+ ++
Sbjct: 13 EAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQ 51
>SPAC22E12.16c |pik1||phosphatidylinositol kinase
Pik1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 851
Score = 27.5 bits (58), Expect = 2.4
Identities = 16/50 (32%), Positives = 26/50 (52%)
Frame = +3
Query: 219 LNLMKGYLLRCFANLPGHIESTQGDTANDFIEGLRHFDKDGNGFISSAEL 368
L+L K + F N+ + S+ DT + G RH D DGNG + +++
Sbjct: 416 LSLSKSF--SSFGNVSLQVPSSHRDTDVVLLSG-RHSDSDGNGALKRSKI 462
>SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3699
Score = 27.1 bits (57), Expect = 3.1
Identities = 16/47 (34%), Positives = 26/47 (55%)
Frame = -2
Query: 419 LYLIITELLSESREQVSQFRRRDEPIAIFVKMAQTLNKVISSVALRA 279
L+L+ L++ +Q + R + IAIF K + +K I SVA+ A
Sbjct: 1377 LHLLFASLVAHKFDQPQHAQTRTKIIAIFFKDLYSPHKEIYSVAIDA 1423
>SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2100
Score = 27.1 bits (57), Expect = 3.1
Identities = 16/48 (33%), Positives = 27/48 (56%)
Frame = +3
Query: 351 ISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQGNINYENFVHLIMQG 494
I A++ LLSTL E D ++EQ+ Q + + Y +F+ ++QG
Sbjct: 42 IVKAQILFLLSTLREDQYDTKLEQIRQLINKNAPRV-YHHFLRRLIQG 88
>SPAC1565.07c |||TATA binding protein interacting protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1220
Score = 27.1 bits (57), Expect = 3.1
Identities = 15/38 (39%), Positives = 24/38 (63%), Gaps = 2/38 (5%)
Frame = +3
Query: 321 RHFDKDGNGFISS-AELRHLLSTLGEK-LSDDEVEQLL 428
RH DK GN F ++ EL +L +G+K L++ +E +L
Sbjct: 670 RHIDKSGNEFTTAYLELLEVLLKVGQKYLAESLLEHIL 707
>SPAC23C11.15 |pst2||Clr6 histone deacetylase complex subunit
Pst2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1075
Score = 26.2 bits (55), Expect = 5.4
Identities = 18/49 (36%), Positives = 26/49 (53%)
Frame = -2
Query: 491 LHDEVNKVLIVDISLRVFLSLQELLYLIITELLSESREQVSQFRRRDEP 345
+HD+V+K S+ VF L LLY + E L +QVS ++R P
Sbjct: 735 MHDDVDKEQFGYSSMYVFFRLFNLLYERLYE-LQRLEDQVSIIQQRIIP 782
>SPBC16D10.06 |||ZIP zinc transporter 2|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 408
Score = 26.2 bits (55), Expect = 5.4
Identities = 20/66 (30%), Positives = 31/66 (46%), Gaps = 1/66 (1%)
Frame = +3
Query: 258 NLPGHIESTQGDTANDFIEGLRHFDKD-GNGFISSAELRHLLSTLGEKLSDDEVEQLLQG 434
NLP + + N +IE L F + G+G I + HLL+ KL D ++ L G
Sbjct: 79 NLPLVLSKITKNRPNVYIEYLYLFARYFGSGVILATAFIHLLAPACNKLYDPCLDDLFGG 138
Query: 435 QEDSQG 452
+ + G
Sbjct: 139 YDWAPG 144
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,108,906
Number of Sequences: 5004
Number of extensions: 62124
Number of successful extensions: 194
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 173
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 193
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 389395636
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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