Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0471
(724 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g20520.1 68418.m02438 expressed protein 73 3e-13
At2g24320.1 68415.m02907 hypothetical protein 40 0.002
At3g30380.1 68416.m03835 expressed protein ; expression supporte... 38 0.005
At1g13610.1 68414.m01597 expressed protein ; expression supporte... 38 0.007
At4g31020.2 68417.m04406 expressed protein 37 0.016
At4g31020.1 68417.m04405 expressed protein 37 0.016
At4g39955.1 68417.m05659 hydrolase, alpha/beta fold family prote... 36 0.036
At5g14980.1 68418.m01757 esterase/lipase/thioesterase family pro... 35 0.047
At5g16120.1 68418.m01883 hydrolase, alpha/beta fold family prote... 35 0.063
At1g15490.1 68414.m01864 hydrolase, alpha/beta fold family prote... 31 1.0
At4g12230.1 68417.m01940 esterase/lipase/thioesterase family pro... 29 2.4
At5g38220.2 68418.m04607 expressed protein 29 4.1
At5g38220.1 68418.m04608 expressed protein 29 4.1
At5g54160.1 68418.m06744 quercetin 3-O-methyltransferase 1 / fla... 28 5.5
At5g45470.1 68418.m05586 expressed protein contains Pfam domain,... 28 5.5
At5g39220.1 68418.m04750 hydrolase, alpha/beta fold family prote... 28 7.2
At4g24760.1 68417.m03545 expressed protein 28 7.2
At3g01690.1 68416.m00101 expressed protein 28 7.2
At2g39410.2 68415.m04837 hydrolase, alpha/beta fold family prote... 28 7.2
At1g73750.1 68414.m08539 expressed protein contains Pfam profile... 28 7.2
At1g66900.1 68414.m07603 expressed protein 28 7.2
At2g39420.1 68415.m04839 esterase/lipase/thioesterase family pro... 27 9.5
>At5g20520.1 68418.m02438 expressed protein
Length = 308
Score = 72.5 bits (170), Expect = 3e-13
Identities = 38/71 (53%), Positives = 51/71 (71%)
Frame = +1
Query: 1 AQCAIDYILERTDVDTSRIILFGRSLGGAVAIDLASRLEYRNKIWALVVENTFTSIPDMA 180
AQ A+D++ RTD+DTSRI++FGRSLGGAV L +K+ AL++ENTFTSI DMA
Sbjct: 135 AQAALDHLSGRTDIDTSRIVVFGRSLGGAVGAVLTK--NNPDKVSALILENTFTSILDMA 192
Query: 181 QIILKWRCLNW 213
++L + L W
Sbjct: 193 GVLLPF--LKW 201
>At2g24320.1 68415.m02907 hypothetical protein
Length = 286
Score = 39.9 bits (89), Expect = 0.002
Identities = 27/84 (32%), Positives = 41/84 (48%)
Frame = +3
Query: 252 EKIAHVISPTLVICGSNDALVPPSMARELYTRCGSICKQMVVIPGGGHDDTWTCREYYPS 431
+KI HV P LVI G+ D +V S + L+ + V GGGH + T EY
Sbjct: 187 DKIRHVTCPVLVIHGTKDDIVNMSHGKRLWELAKDKYDPLWV-KGGGHCNLETYPEYIKH 245
Query: 432 MQQFLVNVPALPSEIGPFFEKNNE 503
M++F+ + L P ++N+E
Sbjct: 246 MRKFMNAMEKLALNNPPNKQQNDE 269
>At3g30380.1 68416.m03835 expressed protein ; expression supported
by MPSS
Length = 399
Score = 38.3 bits (85), Expect = 0.005
Identities = 28/91 (30%), Positives = 45/91 (49%), Gaps = 3/91 (3%)
Frame = +3
Query: 252 EKIAHVISPTLVICGSNDALVPPSMARELYTRCGSICKQMVVIPGGGHDDTWTCREYYPS 431
EKI+ V P LVI G++D +V S ++L+ C + + I GG H D +Y
Sbjct: 194 EKISFVKCPVLVIHGTSDDVVNWSHGKQLFELCKEKYEPL-WIKGGNHCDLELYPQYIKH 252
Query: 432 MQQFLVNV---PALPSEIGPFFEKNNESADL 515
+++F+ + P L + P EK S D+
Sbjct: 253 LRKFVSAIEKSPILRNGPVPLTEKARSSTDI 283
Score = 27.9 bits (59), Expect = 7.2
Identities = 12/29 (41%), Positives = 19/29 (65%)
Frame = +1
Query: 28 ERTDVDTSRIILFGRSLGGAVAIDLASRL 114
E+ V +IL+G+S+G ++LASRL
Sbjct: 131 EKYGVKEQDVILYGQSVGSGPTLELASRL 159
>At1g13610.1 68414.m01597 expressed protein ; expression supported
by MPSS
Length = 351
Score = 37.9 bits (84), Expect = 0.007
Identities = 23/72 (31%), Positives = 37/72 (51%)
Frame = +3
Query: 252 EKIAHVISPTLVICGSNDALVPPSMARELYTRCGSICKQMVVIPGGGHDDTWTCREYYPS 431
+KI V P LVI G++D +V S + L+ C + + + G GH D EY P
Sbjct: 191 DKIHLVECPVLVIHGTDDDVVNISHGKHLWGLCKEKYEPLW-LKGRGHSDIEMSPEYLPH 249
Query: 432 MQQFLVNVPALP 467
+++F+ + LP
Sbjct: 250 LRKFISAIEKLP 261
Score = 33.5 bits (73), Expect = 0.14
Identities = 18/54 (33%), Positives = 33/54 (61%)
Frame = +1
Query: 4 QCAIDYILERTDVDTSRIILFGRSLGGAVAIDLASRLEYRNKIWALVVENTFTS 165
+ A +++ + RIIL+G+S+G +++LASRL ++ ALV+ + F S
Sbjct: 120 EAAYNWLRQTYGTKDERIILYGQSVGSGPSLELASRLP---RLRALVLHSPFLS 170
>At4g31020.2 68417.m04406 expressed protein
Length = 294
Score = 36.7 bits (81), Expect = 0.016
Identities = 23/65 (35%), Positives = 34/65 (52%)
Frame = +3
Query: 252 EKIAHVISPTLVICGSNDALVPPSMARELYTRCGSICKQMVVIPGGGHDDTWTCREYYPS 431
+KI HV S LVI G+ND +V S + L+ + V GGGH + T EY
Sbjct: 195 DKIRHVNSQVLVIHGTNDEIVDLSHGKRLWELAKEKYDPLWV-KGGGHCNLETYPEYIKH 253
Query: 432 MQQFL 446
+++F+
Sbjct: 254 LKKFV 258
>At4g31020.1 68417.m04405 expressed protein
Length = 294
Score = 36.7 bits (81), Expect = 0.016
Identities = 23/65 (35%), Positives = 34/65 (52%)
Frame = +3
Query: 252 EKIAHVISPTLVICGSNDALVPPSMARELYTRCGSICKQMVVIPGGGHDDTWTCREYYPS 431
+KI HV S LVI G+ND +V S + L+ + V GGGH + T EY
Sbjct: 195 DKIRHVNSQVLVIHGTNDEIVDLSHGKRLWELAKEKYDPLWV-KGGGHCNLETYPEYIKH 253
Query: 432 MQQFL 446
+++F+
Sbjct: 254 LKKFV 258
>At4g39955.1 68417.m05659 hydrolase, alpha/beta fold family protein
low similarity to SP|P24640|LIP3_MORSP Lipase 3
precursor (EC 3.1.1.3) (Triacylglycerol lipase)
{Moraxella sp}; contains Pfam profile PF00561:
hydrolase, alpha/beta fold family
Length = 328
Score = 35.5 bits (78), Expect = 0.036
Identities = 21/74 (28%), Positives = 33/74 (44%), Gaps = 1/74 (1%)
Frame = +3
Query: 249 FEKIAHVISPTLVICGSNDALVPPSMARELYTRCGSICKQMVVIPGGGHD-DTWTCREYY 425
F + + PTL+I G D + P +A L G Q+V++ GH + +E Y
Sbjct: 231 FANLPKITQPTLMIWGEEDQVFPVELAHRLKRYLGEDRAQLVLLKKTGHAINEEKPKEMY 290
Query: 426 PSMQQFLVNVPALP 467
M+ FL +P
Sbjct: 291 KHMKSFLCTDAMIP 304
>At5g14980.1 68418.m01757 esterase/lipase/thioesterase family
protein low similarity to monoglyceride lipase from
[Homo sapiens] GI:14594904, [Mus musculus] GI:2632162;
contains Interpro entry IPR000379
Length = 327
Score = 35.1 bits (77), Expect = 0.047
Identities = 14/40 (35%), Positives = 22/40 (55%)
Frame = +3
Query: 276 PTLVICGSNDALVPPSMARELYTRCGSICKQMVVIPGGGH 395
P +++ G +D + P+ ELY RC S K + + PG H
Sbjct: 252 PLMIVHGRDDVVCDPASVEELYRRCSSRDKTIKIYPGMWH 291
>At5g16120.1 68418.m01883 hydrolase, alpha/beta fold family protein
similar to monoglyceride lipase from [Homo sapiens]
GI:14594904, [Mus musculus] GI:2632162; contains Pfam
profile PF00561: hydrolase, alpha/beta fold family
Length = 351
Score = 34.7 bits (76), Expect = 0.063
Identities = 20/65 (30%), Positives = 33/65 (50%), Gaps = 1/65 (1%)
Frame = +3
Query: 204 PKLATTILPQK*VHVFEKIAHVIS-PTLVICGSNDALVPPSMARELYTRCGSICKQMVVI 380
P+L T + + EK +S P L++ G D + PS++RELY + S K++V+
Sbjct: 250 PRLRTAVEMLRTTQDIEKQLQEVSLPILILHGEADTVTDPSVSRELYEKAKSPDKKIVLY 309
Query: 381 PGGGH 395
H
Sbjct: 310 ENAYH 314
>At1g15490.1 68414.m01864 hydrolase, alpha/beta fold family protein
Contains PF 00561 alpha/beta hydrolase fold
Length = 648
Score = 30.7 bits (66), Expect = 1.0
Identities = 25/100 (25%), Positives = 49/100 (49%), Gaps = 3/100 (3%)
Frame = +3
Query: 177 GTDHFKMEMPKLATTIL--PQK*VHVFEKIAHVISPTLVICGSNDALVPPSMARELYTRC 350
G D E+ +L++ ++ PQ + + + ++ P LVI G+ DALVP ++ + ++
Sbjct: 548 GWDEALHEIGRLSSEMVLAPQNAASLLKAVENL--PVLVIAGAEDALVPLKSSQGMASKL 605
Query: 351 GSICKQMVVIPGGGHDDTWTC-REYYPSMQQFLVNVPALP 467
+ ++V I G GH C + +M F+ + P
Sbjct: 606 --LNSRLVAISGCGHLPHEECPKALLAAMSPFITRLVIRP 643
>At4g12230.1 68417.m01940 esterase/lipase/thioesterase family
protein low similarity to
2-hydroxy-6-ketonona-2,4-dienedoic acid hydrolase; OhpC
[Rhodococcus sp.] GI:8926386; contains Interpro entry
IPR000379
Length = 392
Score = 29.5 bits (63), Expect = 2.4
Identities = 20/65 (30%), Positives = 30/65 (46%)
Frame = +1
Query: 40 VDTSRIILFGRSLGGAVAIDLASRLEYRNKIWALVVENTFTSIPDMAQIILKWRCLNWLP 219
+D + L+G SLGG +A A R K +LV+ NT+ A + ++W P
Sbjct: 95 IDVHHVHLYGTSLGGFLAQLFAHHRPRRVK--SLVLSNTYLDTRTFATAMPWAPFVSWTP 152
Query: 220 QFCHK 234
F K
Sbjct: 153 SFLLK 157
>At5g38220.2 68418.m04607 expressed protein
Length = 238
Score = 28.7 bits (61), Expect = 4.1
Identities = 13/28 (46%), Positives = 18/28 (64%)
Frame = +1
Query: 28 ERTDVDTSRIILFGRSLGGAVAIDLASR 111
E V ++IL+G+S+G IDLASR
Sbjct: 130 EHYGVKDDQLILYGQSVGSGPTIDLASR 157
>At5g38220.1 68418.m04608 expressed protein
Length = 336
Score = 28.7 bits (61), Expect = 4.1
Identities = 13/28 (46%), Positives = 18/28 (64%)
Frame = +1
Query: 28 ERTDVDTSRIILFGRSLGGAVAIDLASR 111
E V ++IL+G+S+G IDLASR
Sbjct: 130 EHYGVKDDQLILYGQSVGSGPTIDLASR 157
>At5g54160.1 68418.m06744 quercetin 3-O-methyltransferase 1 /
flavonol 3-O-methyltransferase 1 / caffeic
acid/5-hydroxyferulic acid O-methyltransferase (OMT1)
identical to O-methyltransferase 1 [Arabidopsis
thaliana][GI:2781394], SP|Q9FK25 Quercetin
3-O-methyltransferase 1 (EC 2.1.1.76) (AtOMT1) (Flavonol
3- O-methyltransferase 1) (Caffeic acid/5-hydroxyferulic
acid O- methyltransferase) {Arabidopsis thaliana}
Length = 363
Score = 28.3 bits (60), Expect = 5.5
Identities = 14/35 (40%), Positives = 18/35 (51%), Gaps = 3/35 (8%)
Frame = +1
Query: 157 FTSIPDMAQIILKWRCLNWLPQFC---HKNKYMSL 252
F S+P I +KW C +W + C KN Y SL
Sbjct: 251 FVSVPKGDAIFMKWICHDWSDEHCVKFLKNCYESL 285
>At5g45470.1 68418.m05586 expressed protein contains Pfam domain,
PF04578: Protein of unknown function, DUF594
Length = 866
Score = 28.3 bits (60), Expect = 5.5
Identities = 19/75 (25%), Positives = 30/75 (40%), Gaps = 8/75 (10%)
Frame = +1
Query: 34 TDVDTSRIILFGRSLGGAVAIDLASRLEYRNKIWALVVENTFTSIPDMAQI--------I 189
TD + +++ + +D S L + W ++ PD Q +
Sbjct: 334 TDFHGADVVVTYTLFAVGLVLDFISILLFLFSDWTCAAYSSLKDDPDELQSWQERCFNWL 393
Query: 190 LKWRCLNWLPQFCHK 234
LK+R L W PQ CHK
Sbjct: 394 LKFRKLRWTPQECHK 408
>At5g39220.1 68418.m04750 hydrolase, alpha/beta fold family protein
contains Pfam profile: PF00561 alpha/beta hydrolase fold
Length = 330
Score = 27.9 bits (59), Expect = 7.2
Identities = 15/51 (29%), Positives = 24/51 (47%)
Frame = +3
Query: 243 HVFEKIAHVISPTLVICGSNDALVPPSMARELYTRCGSICKQMVVIPGGGH 395
+V I H+ TLV+C ND +V ++ +L C + +P GH
Sbjct: 259 NVASHIKHIDHKTLVVCSENDQIVSNQLSVKLL--CELANAVLREVPDSGH 307
>At4g24760.1 68417.m03545 expressed protein
Length = 365
Score = 27.9 bits (59), Expect = 7.2
Identities = 13/37 (35%), Positives = 20/37 (54%)
Frame = +1
Query: 4 QCAIDYILERTDVDTSRIILFGRSLGGAVAIDLASRL 114
+ A + E IIL+G+S+G +DLA+RL
Sbjct: 124 EAAYKCLEENYGAKQENIILYGQSVGSGPTVDLAARL 160
>At3g01690.1 68416.m00101 expressed protein
Length = 361
Score = 27.9 bits (59), Expect = 7.2
Identities = 11/20 (55%), Positives = 16/20 (80%)
Frame = +1
Query: 55 IILFGRSLGGAVAIDLASRL 114
+IL+G+S+G +DLASRL
Sbjct: 141 VILYGQSVGSGPTLDLASRL 160
>At2g39410.2 68415.m04837 hydrolase, alpha/beta fold family protein
similar to monoglyceride lipase from [Homo sapiens]
GI:14594904, [Mus musculus] GI:2632162; contains Pfam
profile PF00561: hydrolase, alpha/beta fold family
Length = 317
Score = 27.9 bits (59), Expect = 7.2
Identities = 14/48 (29%), Positives = 26/48 (54%)
Frame = +3
Query: 252 EKIAHVISPTLVICGSNDALVPPSMARELYTRCGSICKQMVVIPGGGH 395
+++ V P LV+ G +D + ++++ELY S K + + PG H
Sbjct: 222 KRLQEVSLPFLVLHGDDDKVTDKAVSQELYKVALSADKTLKLYPGMWH 269
>At1g73750.1 68414.m08539 expressed protein contains Pfam profile:
PF00561 alpha/beta hydrolase fold
Length = 452
Score = 27.9 bits (59), Expect = 7.2
Identities = 15/55 (27%), Positives = 25/55 (45%)
Frame = +1
Query: 10 AIDYILERTDVDTSRIILFGRSLGGAVAIDLASRLEYRNKIWALVVENTFTSIPD 174
A+DY+ +T +++ G S+GG + L SR ++ L T S D
Sbjct: 216 AMDYVRTQTKSKDGKLLAVGHSMGGILLYALLSRCGFKGMDSGLAGVTTLASTFD 270
>At1g66900.1 68414.m07603 expressed protein
Length = 272
Score = 27.9 bits (59), Expect = 7.2
Identities = 11/28 (39%), Positives = 19/28 (67%)
Frame = +1
Query: 28 ERTDVDTSRIILFGRSLGGAVAIDLASR 111
E+ V ++I++G+S+G +DLASR
Sbjct: 133 EKYGVKDDQLIVYGQSVGSGPTVDLASR 160
>At2g39420.1 68415.m04839 esterase/lipase/thioesterase family
protein similar to monoglyceride lipase from [Homo
sapiens] GI:14594904, [Mus musculus] GI:2632162;
contains Interpro entry IPR000379
Length = 317
Score = 27.5 bits (58), Expect = 9.5
Identities = 13/48 (27%), Positives = 25/48 (52%)
Frame = +3
Query: 252 EKIAHVISPTLVICGSNDALVPPSMARELYTRCGSICKQMVVIPGGGH 395
+++ V P +V+ G +D + +++R+LY S K + PG H
Sbjct: 222 KRLNEVSLPFIVLHGEDDKVTDKAVSRQLYEVASSSDKTFKLYPGMWH 269
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,111,534
Number of Sequences: 28952
Number of extensions: 383308
Number of successful extensions: 886
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 851
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 885
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1575119672
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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