Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0453
(561 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g20060.2 68418.m02389 phospholipase/carboxylesterase family p... 67 7e-12
At5g20060.1 68418.m02388 phospholipase/carboxylesterase family p... 67 7e-12
At1g52700.1 68414.m05952 phospholipase/carboxylesterase family p... 63 1e-10
At3g15650.1 68416.m01984 phospholipase/carboxylesterase family p... 62 2e-10
At4g22300.1 68417.m03225 phospholipase/carboxylesterase family p... 51 6e-07
At1g52695.1 68414.m05951 phospholipase/carboxylesterase family p... 45 3e-05
At1g47786.1 68414.m05316 acyl-protein thioesterase-related simil... 45 3e-05
At1g51300.1 68414.m05769 acyl-protein thioesterase-related cont... 38 0.006
At1g47780.1 68414.m05314 acyl-protein thioesterase-related conta... 37 0.008
At3g50590.1 68416.m05533 transducin family protein / WD-40 repea... 29 2.8
At2g46210.1 68415.m05746 delta-8 sphingolipid desaturase, putati... 29 2.8
At2g14800.1 68415.m01677 hypothetical protein 27 6.5
At1g60160.1 68414.m06777 potassium transporter family protein si... 27 6.5
At2g23560.1 68415.m02812 hydrolase, alpha/beta fold family prote... 27 8.6
>At5g20060.2 68418.m02389 phospholipase/carboxylesterase family
protein similar to lysophospholipase II [Mus musculus]
GI:4589453; contains Pfam profile PF02230:
Phospholipase/Carboxylesterase family
Length = 252
Score = 67.3 bits (157), Expect = 7e-12
Identities = 24/63 (38%), Positives = 42/63 (66%)
Frame = +1
Query: 61 IIAAQARHTASLIFLHGLGDTGHGWASTIAGIRGPHVKVICPTASTMPVTLNNGFRMPSW 240
++ + +H A++++LHGLGD G W+ + + P++K ICPTA + P++L GF +W
Sbjct: 25 VVRPKGKHQATIVWLHGLGDNGSSWSQLLETLPLPNIKWICPTAPSQPISLFGGFPSTAW 84
Query: 241 FDL 249
FD+
Sbjct: 85 FDV 87
>At5g20060.1 68418.m02388 phospholipase/carboxylesterase family
protein similar to lysophospholipase II [Mus musculus]
GI:4589453; contains Pfam profile PF02230:
Phospholipase/Carboxylesterase family
Length = 252
Score = 67.3 bits (157), Expect = 7e-12
Identities = 24/63 (38%), Positives = 42/63 (66%)
Frame = +1
Query: 61 IIAAQARHTASLIFLHGLGDTGHGWASTIAGIRGPHVKVICPTASTMPVTLNNGFRMPSW 240
++ + +H A++++LHGLGD G W+ + + P++K ICPTA + P++L GF +W
Sbjct: 25 VVRPKGKHQATIVWLHGLGDNGSSWSQLLETLPLPNIKWICPTAPSQPISLFGGFPSTAW 84
Query: 241 FDL 249
FD+
Sbjct: 85 FDV 87
>At1g52700.1 68414.m05952 phospholipase/carboxylesterase family
protein similar to lysophospholipase I [Mus musculus]
GI:1864159; contains Pfam profile PF02230:
Phospholipase/Carboxylesterase family
Length = 255
Score = 62.9 bits (146), Expect = 1e-10
Identities = 25/63 (39%), Positives = 40/63 (63%)
Frame = +1
Query: 61 IIAAQARHTASLIFLHGLGDTGHGWASTIAGIRGPHVKVICPTASTMPVTLNNGFRMPSW 240
++ + +H A+L++LHGLGD G + + + P++K ICPTA + PVT GF +W
Sbjct: 25 VVRPKGKHQATLVWLHGLGDNGSSSSQLMDSLHLPNIKWICPTAPSRPVTSLGGFTCTAW 84
Query: 241 FDL 249
FD+
Sbjct: 85 FDV 87
>At3g15650.1 68416.m01984 phospholipase/carboxylesterase family
protein low similarity to lysophospholipase I [Mus
musculus] GI:1864159; contains Pfam profile PF02230:
Phospholipase/Carboxylesterase family
Length = 255
Score = 62.5 bits (145), Expect = 2e-10
Identities = 24/63 (38%), Positives = 41/63 (65%)
Frame = +1
Query: 61 IIAAQARHTASLIFLHGLGDTGHGWASTIAGIRGPHVKVICPTASTMPVTLNNGFRMPSW 240
++ + +H A++++LHGLGD G + + + P++K ICPTA + PV+L GF +W
Sbjct: 25 VVRPKGKHQATIVWLHGLGDNGSSSSQLLESLPLPNIKWICPTAPSRPVSLLGGFPCTAW 84
Query: 241 FDL 249
FD+
Sbjct: 85 FDV 87
>At4g22300.1 68417.m03225 phospholipase/carboxylesterase family
protein similar to acyl-protein thioesterase-1 [Homo
sapiens] GI:9965372; contains Pfam profile PF02230:
Phospholipase/Carboxylesterase family
Length = 471
Score = 50.8 bits (116), Expect = 6e-07
Identities = 24/70 (34%), Positives = 35/70 (50%)
Frame = +3
Query: 255 LDATAPEDEDGIERATDLVHGLIADEVKAGVPADKVLLGGFSQGGXXXXXXXXTYPERLA 434
L A +P+DE + +A VH +I E+ G+ + V + GFSQGG YP+ +
Sbjct: 350 LTAGSPKDESSLLKAVKNVHAIIDKEIAGGINPENVYICGFSQGGALTLASVLLYPKTIG 409
Query: 435 GVMSLSCWXP 464
G S W P
Sbjct: 410 GGAVFSGWIP 419
Score = 46.0 bits (104), Expect = 2e-05
Identities = 23/66 (34%), Positives = 30/66 (45%)
Frame = +3
Query: 267 APEDEDGIERATDLVHGLIADEVKAGVPADKVLLGGFSQGGXXXXXXXXTYPERLAGVMS 446
+P DE + A VH +I E+ G + V + G SQGG YP+ L G
Sbjct: 69 SPIDESSVLEAVKNVHAIIDQEIAEGTNPENVFICGLSQGGALTLASVLLYPKTLGGGAV 128
Query: 447 LSCWXP 464
LS W P
Sbjct: 129 LSGWVP 134
Score = 39.1 bits (87), Expect = 0.002
Identities = 21/55 (38%), Positives = 30/55 (54%), Gaps = 3/55 (5%)
Frame = +1
Query: 94 LIFLHGLGDTGHGWASTIAGIRGP---HVKVICPTASTMPVTLNNGFRMPSWFDL 249
+++LHGLGD+G R + K + P+A PV+ N G MPSWFD+
Sbjct: 291 ILWLHGLGDSGPANEPIKTLFRSQEFRNTKWLFPSAPPNPVSCNYGAVMPSWFDI 345
Score = 37.1 bits (82), Expect = 0.008
Identities = 20/55 (36%), Positives = 29/55 (52%), Gaps = 3/55 (5%)
Frame = +1
Query: 94 LIFLHGLGDTGHGWASTIAGIRGPHVKV---ICPTASTMPVTLNNGFRMPSWFDL 249
+++LHGLGD+G + + + P+A PVT NNG M SWFD+
Sbjct: 6 ILWLHGLGDSGPANEPIQTQFKSSELSNASWLFPSAPFNPVTCNNGAVMRSWFDV 60
>At1g52695.1 68414.m05951 phospholipase/carboxylesterase family
protein contains Pfam profile: PF02230
phospholipase/carboxylesterase; supported by full length
cDNA gi:26450919 from [Arabidopsis thaliana]
Length = 231
Score = 45.2 bits (102), Expect = 3e-05
Identities = 20/56 (35%), Positives = 32/56 (57%)
Frame = +1
Query: 82 HTASLIFLHGLGDTGHGWASTIAGIRGPHVKVICPTASTMPVTLNNGFRMPSWFDL 249
H A++++LH +G+TG + +R P++K ICPTA VT G +W D+
Sbjct: 25 HKATIVWLHDVGNTGFNSLEPLQNLRLPNIKWICPTAPRRRVTSLGGEITNAWCDI 80
>At1g47786.1 68414.m05316 acyl-protein thioesterase-related similar
to hypothetical protein GB:AAD55623 GI:5903064 from
[Arabidopsis thaliana] contains similarity to
acyl-protein thioesterase-1 [Homo sapiens]
gi|9965372|gb|AAG10063
Length = 186
Score = 45.2 bits (102), Expect = 3e-05
Identities = 19/56 (33%), Positives = 31/56 (55%)
Frame = +1
Query: 82 HTASLIFLHGLGDTGHGWASTIAGIRGPHVKVICPTASTMPVTLNNGFRMPSWFDL 249
H A++++LH +G+T + ++K ICPTA PVT+ G +WFD+
Sbjct: 55 HKATIVWLHDIGETSANSVRFARQLGLRNIKWICPTAPRRPVTILGGMETNAWFDI 110
>At1g51300.1 68414.m05769 acyl-protein thioesterase-related
contains similarity to acyl-protein thioesterase-1 [Homo
sapiens] gi|9965372|gb|AAG10063
Length = 212
Score = 37.5 bits (83), Expect = 0.006
Identities = 18/56 (32%), Positives = 33/56 (58%)
Frame = +1
Query: 58 VIIAAQARHTASLIFLHGLGDTGHGWASTIAGIRGPHVKVICPTASTMPVTLNNGF 225
V + +ARH A++++LH L ++G+ + + +VK ICP++ P+ N GF
Sbjct: 28 VTVTPRARHQATIVWLHDLNESGYDSSELVKSFSLYNVKWICPSS---PLISNVGF 80
>At1g47780.1 68414.m05314 acyl-protein thioesterase-related contains
similarity to acyl-protein thioesterase-1 [Homo sapiens]
gi|9965372|gb|AAG10063 contains similarity to
acyl-protein thioesterase-1 [Homo sapiens]
gi|9965372|gb|AAG10063
Length = 126
Score = 37.1 bits (82), Expect = 0.008
Identities = 15/39 (38%), Positives = 21/39 (53%)
Frame = +1
Query: 133 WASTIAGIRGPHVKVICPTASTMPVTLNNGFRMPSWFDL 249
W + G +VK ICPTA P+T+ G +WFD+
Sbjct: 12 WVLKMYGWMNKNVKWICPTAPRRPLTILGGMETNAWFDI 50
>At3g50590.1 68416.m05533 transducin family protein / WD-40 repeat
family protein contains 3 WD-40 repeats (PF00400); some
similarity to s-tomosyn isoform (GI:4689231)[Rattus
norvegicus]; contains non-consensus AT-AC splice sites
at intron 18
Length = 1606
Score = 28.7 bits (61), Expect = 2.8
Identities = 16/45 (35%), Positives = 23/45 (51%), Gaps = 2/45 (4%)
Frame = -2
Query: 164 GPLMPAIVLAHP*PVSPKPCKNISDAVCLA--CAAIITGFGSILQ 36
G L ++LAHP +SPK K I CL ++ GF ++ Q
Sbjct: 769 GALNDRLLLAHPTDISPKQKKGIEIKSCLVGLLEPLLIGFSTMQQ 813
>At2g46210.1 68415.m05746 delta-8 sphingolipid desaturase, putative
similar to delta-8 sphingolipid desaturase GI:3819708
from [Brassica napus]
Length = 449
Score = 28.7 bits (61), Expect = 2.8
Identities = 10/37 (27%), Positives = 19/37 (51%)
Frame = +1
Query: 166 HVKVICPTASTMPVTLNNGFRMPSWFDLRTWTLQLLK 276
H++ + P + N +R SW++ WT++ LK
Sbjct: 387 HLRTVSPVVKELCKKHNLPYRSLSWWEANVWTIRTLK 423
>At2g14800.1 68415.m01677 hypothetical protein
Length = 580
Score = 27.5 bits (58), Expect = 6.5
Identities = 17/46 (36%), Positives = 26/46 (56%), Gaps = 1/46 (2%)
Frame = -2
Query: 326 SNQPMDEISGSLNTVFIFRSCSVQVLKSNHEGIR-KPLLSVTGIVE 192
SNQ +E+SG T S Q+L S+ E +R L+++T +VE
Sbjct: 72 SNQVDNEVSGENGTKDDATLLSPQLLVSDSENLRNNELMAITSVVE 117
>At1g60160.1 68414.m06777 potassium transporter family protein
similar to potassium transporter HAK2p [Mesembryanthemum
crystallinum] gi|14091471|gb|AAK53759; KUP/HAK/KT
Transporter family member, PMID:11500563; contains Pfam
profile PF02705: K+ potassium transporter
Length = 827
Score = 27.5 bits (58), Expect = 6.5
Identities = 12/48 (25%), Positives = 24/48 (50%)
Frame = +1
Query: 4 KLAHIDFPVINWRMEPNPVIIAAQARHTASLIFLHGLGDTGHGWASTI 147
++ I PVINW + +++ + R T + +G+ + G ST+
Sbjct: 451 RIGQIYIPVINWFLMIMCILVVSIFRSTTHIANAYGIAEVGVMMVSTV 498
>At2g23560.1 68415.m02812 hydrolase, alpha/beta fold family protein
similar to ethylene-induced esterase [Citrus sinensis]
GI:14279437, polyneuridine aldehyde esterase [Rauvolfia
serpentina] GI:6651393; contains Pfam profile PF00561:
hydrolase, alpha/beta fold family
Length = 260
Score = 27.1 bits (57), Expect = 8.6
Identities = 15/61 (24%), Positives = 26/61 (42%)
Frame = +3
Query: 282 DGIERATDLVHGLIADEVKAGVPADKVLLGGFSQGGXXXXXXXXTYPERLAGVMSLSCWX 461
D I+ D L+ G DKV+L S GG +P ++A ++ ++ +
Sbjct: 52 DEIQTLKDYCKPLLEFLSSLGSDDDKVILVAHSMGGISASLAADIFPSKVAAIVFVAAFM 111
Query: 462 P 464
P
Sbjct: 112 P 112
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,385,507
Number of Sequences: 28952
Number of extensions: 255112
Number of successful extensions: 670
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 645
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 667
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1072696904
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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