BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0446
(860 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC21H7.06c |||inositol metabolism protein Opi10 |Schizosacchar... 66 8e-12
SPAPB1E7.02c |mcl1|slr3|DNA polymerase alpha accessory factor Mc... 27 4.5
SPAC2F7.15 |rsm24||mitochondrial ribosomal protein subunit S24|S... 26 7.9
>SPBC21H7.06c |||inositol metabolism protein Opi10
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 200
Score = 65.7 bits (153), Expect = 8e-12
Identities = 30/77 (38%), Positives = 45/77 (58%)
Frame = +2
Query: 74 VFGLIVSGRLVHTDFSPIAETRFLTTITEADSINHVAVFLTGAVPLPPGTAAMVYWSWPD 253
+FG I +GRLV T+ +A+ +F+ + A+S+NH+ VFL P P G A VY+ WP
Sbjct: 1 MFGAICAGRLVQTNLQQVADNQFVFQLDSAESLNHIVVFLLPNSPFPVGMGAKVYFQWPG 60
Query: 254 QPHLLTGSYLVTFRTSS 304
+P G YL + S+
Sbjct: 61 KPFQFLG-YLTNEKPSA 76
Score = 38.3 bits (85), Expect = 0.001
Identities = 20/60 (33%), Positives = 29/60 (48%), Gaps = 6/60 (10%)
Frame = +3
Query: 495 ICSEKLENLVNFVASFSVTQDQMTPT------PGVSYIPLNTLHTWYQNFERRLQQNPNF 656
+ + L NL NF+ASF+ +Q P ++IPL W+ F +L NPNF
Sbjct: 134 VAQKILTNLYNFLASFATSQLPPNSIGLGDLRPNDTFIPLRVFQDWHAKFLNKLSNNPNF 193
Score = 33.5 bits (73), Expect = 0.039
Identities = 20/57 (35%), Positives = 31/57 (54%)
Frame = +1
Query: 271 WQLLGHISNIKPSAIFKISNLKKLHELTNENKFMGTFGQQQICHNAQIGISIEPEAN 441
+Q LG+++N KPSAIF++ N + L+ +G A +GIS+EP N
Sbjct: 63 FQFLGYLTNEKPSAIFRLKN--TIQTLSENENCVGI--------TAMLGISVEPLTN 109
>SPAPB1E7.02c |mcl1|slr3|DNA polymerase alpha accessory factor
Mcl1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 815
Score = 26.6 bits (56), Expect = 4.5
Identities = 16/53 (30%), Positives = 28/53 (52%), Gaps = 2/53 (3%)
Frame = +1
Query: 352 TNENKFMGTFGQQQICHNAQIGISIEPEA--NVQLLASSVALQETNNYVTFAQ 504
T + KF+ T G Q+ Q+G EP++ N Q + +A+ E N + T ++
Sbjct: 22 TRDGKFLLTVGSNQVIRKFQVGSDEEPDSIDNHQDPITGIAVAE-NYFCTCSE 73
>SPAC2F7.15 |rsm24||mitochondrial ribosomal protein subunit
S24|Schizosaccharomyces pombe|chr 1|||Manual
Length = 258
Score = 25.8 bits (54), Expect = 7.9
Identities = 14/52 (26%), Positives = 26/52 (50%), Gaps = 1/52 (1%)
Frame = +3
Query: 492 DICSEKLENLVNFVASF-SVTQDQMTPTPGVSYIPLNTLHTWYQNFERRLQQ 644
D S L+N + + S+ ++ S++PLNT H Y+ ++R+ Q
Sbjct: 171 DKYSSALQNKYHLIKILTSLIEESKRNAEKFSHVPLNTGHWKYKKCDKRMPQ 222
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,351,476
Number of Sequences: 5004
Number of extensions: 65754
Number of successful extensions: 159
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 149
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 158
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 428468660
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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