Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0438
(808 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g61530.2 68418.m07721 small G protein family protein / RhoGAP... 40 0.002
At5g61530.1 68418.m07720 small G protein family protein / RhoGAP... 40 0.002
At5g12150.1 68418.m01426 pleckstrin homology (PH) domain-contain... 39 0.004
At4g24580.1 68417.m03522 pleckstrin homology (PH) domain-contain... 37 0.014
At5g22400.1 68418.m02613 rac GTPase activating protein, putative... 33 0.17
At4g03100.1 68417.m00418 rac GTPase activating protein, putative... 33 0.17
At3g11490.1 68416.m01401 rac GTPase activating protein, putative... 32 0.39
At1g08340.1 68414.m00922 rac GTPase activating protein, putative... 31 1.2
At5g52250.1 68418.m06485 transducin family protein / WD-40 repea... 29 2.7
At4g21410.1 68417.m03093 protein kinase family protein contains ... 29 2.7
At2g27440.1 68415.m03316 rac GTPase activating protein, putative... 29 2.7
At3g29033.1 68416.m03629 glycine-rich protein 28 6.3
At3g18290.1 68416.m02326 zinc finger protein-related weak alignm... 28 6.3
At2g46710.1 68415.m05828 rac GTPase activating protein, putative... 28 6.3
At1g75800.1 68414.m08805 pathogenesis-related thaumatin family p... 28 6.3
At1g15780.1 68414.m01893 expressed protein 28 6.3
>At5g61530.2 68418.m07721 small G protein family protein / RhoGAP
family protein contains Pfam domain, PF00620: RhoGAP
domain
Length = 367
Score = 39.9 bits (89), Expect = 0.002
Identities = 24/80 (30%), Positives = 42/80 (52%), Gaps = 2/80 (2%)
Frame = +2
Query: 20 IPPIVRQCVEFLSRPDALETEGIFRRSANMVTVKQLQNSCNQGEPISFRD--DPHIAAVL 193
IP I+ +C ++L L + +F+ + ++QL ++ NQ S + +P A L
Sbjct: 154 IPLILVKCADYLILT-GLNSPNLFKAEGDRKLIQQLVSAYNQDPRASIPEGVNPVDVAAL 212
Query: 194 LKTFLRDLEEPVLTYDLYEE 253
LK +L L P+ T++LY E
Sbjct: 213 LKYYLASLPTPLTTFELYNE 232
>At5g61530.1 68418.m07720 small G protein family protein / RhoGAP
family protein contains Pfam domain, PF00620: RhoGAP
domain
Length = 376
Score = 39.9 bits (89), Expect = 0.002
Identities = 24/80 (30%), Positives = 42/80 (52%), Gaps = 2/80 (2%)
Frame = +2
Query: 20 IPPIVRQCVEFLSRPDALETEGIFRRSANMVTVKQLQNSCNQGEPISFRD--DPHIAAVL 193
IP I+ +C ++L L + +F+ + ++QL ++ NQ S + +P A L
Sbjct: 154 IPLILVKCADYLILT-GLNSPNLFKAEGDRKLIQQLVSAYNQDPRASIPEGVNPVDVAAL 212
Query: 194 LKTFLRDLEEPVLTYDLYEE 253
LK +L L P+ T++LY E
Sbjct: 213 LKYYLASLPTPLTTFELYNE 232
Score = 35.9 bits (79), Expect = 0.032
Identities = 16/46 (34%), Positives = 26/46 (56%)
Frame = +1
Query: 313 LERLPIENYKLLKYLFQFLWKVKDRSCLNKMTCSNLAVVFGPNLAW 450
L++L NY L+++ L +V +S LNKM +LA+ P + W
Sbjct: 247 LQKLSNVNYNTLEFITALLLRVSQKSLLNKMDSHSLAMEMAPVIMW 292
>At5g12150.1 68418.m01426 pleckstrin homology (PH) domain-containing
protein / RhoGAP domain-containing protein weak
similarity to glucocorticoid receptor DNA binding factor
1 [Canis familiaris] GI:23266717; contains Pfam profiles
PF00169: PH domain, PF00620: RhoGAP domain
Length = 827
Score = 38.7 bits (86), Expect = 0.004
Identities = 23/70 (32%), Positives = 37/70 (52%), Gaps = 1/70 (1%)
Frame = +2
Query: 11 IDGIPPIVRQCVEFLSRPDALETEGIFRRSANMVTVKQLQNSCNQGE-PISFRDDPHIAA 187
IDG P + + ++FL + EGI R+SA++ V++ QG+ S +DPH+
Sbjct: 177 IDGSPSFLEKALQFLETYGT-KVEGILRQSADVEEVERRVQEYEQGKTEFSPEEDPHVVG 235
Query: 188 VLLKTFLRDL 217
+K LR L
Sbjct: 236 DCVKHVLRQL 245
>At4g24580.1 68417.m03522 pleckstrin homology (PH) domain-containing
protein-related / RhoGAP domain-containing protein
contains Pfam domain, PF00620: RhoGAP domain
Length = 902
Score = 37.1 bits (82), Expect = 0.014
Identities = 22/70 (31%), Positives = 37/70 (52%), Gaps = 1/70 (1%)
Frame = +2
Query: 11 IDGIPPIVRQCVEFLSRPDALETEGIFRRSANMVTVKQLQNSCNQGE-PISFRDDPHIAA 187
+DG P + + + F+ + EGI R++A++ V+ +G+ S +D HI A
Sbjct: 218 VDGAPSFLEKALRFVEN-HGVRIEGILRQAADVDDVEHRIREYEKGKNEFSPEEDAHIIA 276
Query: 188 VLLKTFLRDL 217
LK FLR+L
Sbjct: 277 DCLKYFLREL 286
>At5g22400.1 68418.m02613 rac GTPase activating protein, putative
similar to rac GTPase activating protein 1 [Lotus
japonicus] GI:3695059; contains Pfam profile PF00620:
RhoGAP domain
Length = 466
Score = 33.5 bits (73), Expect = 0.17
Identities = 23/71 (32%), Positives = 34/71 (47%)
Frame = +2
Query: 20 IPPIVRQCVEFLSRPDALETEGIFRRSANMVTVKQLQNSCNQGEPISFRDDPHIAAVLLK 199
+P I+ L L+ EGIFR +A + ++ N+G I R D H A L+K
Sbjct: 178 VPTILLLMQNCLYSQGGLQAEGIFRLTAENSEEEAVREQLNRGF-IPERIDVHCLAGLIK 236
Query: 200 TFLRDLEEPVL 232
+ R+L VL
Sbjct: 237 AWFRELPTSVL 247
Score = 30.3 bits (65), Expect = 1.6
Identities = 18/50 (36%), Positives = 26/50 (52%)
Frame = +1
Query: 295 NVKILILERLPIENYKLLKYLFQFLWKVKDRSCLNKMTCSNLAVVFGPNL 444
NV+++ L LP LL + + V LNKM N+A+VF PN+
Sbjct: 264 NVELVRL--LPPTEAALLDWAINLMADVVQYEHLNKMNSRNIAMVFAPNM 311
>At4g03100.1 68417.m00418 rac GTPase activating protein, putative
similar to rac GTPase activating protein 3 [Lotus
japonicus] GI:3695063; contains Pfam profile PF00620:
RhoGAP domain
Length = 430
Score = 33.5 bits (73), Expect = 0.17
Identities = 25/85 (29%), Positives = 39/85 (45%)
Frame = +2
Query: 14 DGIPPIVRQCVEFLSRPDALETEGIFRRSANMVTVKQLQNSCNQGEPISFRDDPHIAAVL 193
+ +P I+ E L L+ EGIFR + + +++ N+G + D H A L
Sbjct: 139 NSVPTILLLMQERLYSQQGLKAEGIFRINPENSQEEHVRDQLNRG-IVPENIDVHCLAGL 197
Query: 194 LKTFLRDLEEPVLTYDLYEETSNFN 268
+K + R+L VL EE N N
Sbjct: 198 IKAWFRELPSGVLDGLSPEEVLNCN 222
Score = 28.7 bits (61), Expect = 4.8
Identities = 16/46 (34%), Positives = 24/46 (52%)
Frame = +1
Query: 307 LILERLPIENYKLLKYLFQFLWKVKDRSCLNKMTCSNLAVVFGPNL 444
LI + P E+ LL + + V + NKM N+A+VF PN+
Sbjct: 230 LIKQLKPTES-ALLNWAVDLMADVVEEEESNKMNARNIAMVFAPNM 274
>At3g11490.1 68416.m01401 rac GTPase activating protein, putative
similar to rac GTPase activating protein 1 GB:AAC62624
GI:3695059 [Lotus japonicus]; contains Pfam profile
PF00620: RhoGAP domain
Length = 435
Score = 32.3 bits (70), Expect = 0.39
Identities = 15/45 (33%), Positives = 23/45 (51%)
Frame = +1
Query: 310 ILERLPIENYKLLKYLFQFLWKVKDRSCLNKMTCSNLAVVFGPNL 444
++ LP LL + + V + LNKM N+A+VF PN+
Sbjct: 243 LVRLLPSTEASLLDWAINLMADVVEMEQLNKMNARNIAMVFAPNM 287
Score = 29.9 bits (64), Expect = 2.1
Identities = 21/71 (29%), Positives = 31/71 (43%)
Frame = +2
Query: 20 IPPIVRQCVEFLSRPDALETEGIFRRSANMVTVKQLQNSCNQGEPISFRDDPHIAAVLLK 199
+P I+ L L EGIFR + + ++ N+G I D H A L+K
Sbjct: 154 VPTILLMMQSHLYSRGGLRVEGIFRINGENGQEEYIREELNKG-IIPDNIDVHCLASLIK 212
Query: 200 TFLRDLEEPVL 232
+ R+L VL
Sbjct: 213 AWFRELPSGVL 223
>At1g08340.1 68414.m00922 rac GTPase activating protein, putative
similar to rac GTPase activating protein 1 GI:3695059
from [Lotus japonicus]; contains Pfam profile PF00620:
RhoGAP domain
Length = 331
Score = 30.7 bits (66), Expect = 1.2
Identities = 15/48 (31%), Positives = 24/48 (50%)
Frame = +1
Query: 304 ILILERLPIENYKLLKYLFQFLWKVKDRSCLNKMTCSNLAVVFGPNLA 447
+ ++ LP LL + + V +NKM NLA+VF PN++
Sbjct: 151 VKVVRLLPQTEASLLNWAINLMADVIQFEHVNKMNSRNLALVFAPNMS 198
>At5g52250.1 68418.m06485 transducin family protein / WD-40 repeat
family protein contains 7 WD-40 repeats (PF00400);
similar to photomorphogenesis repressor PnCOP1
(GI:11127996) [Ipomoea nil]
Length = 385
Score = 29.5 bits (63), Expect = 2.7
Identities = 18/51 (35%), Positives = 25/51 (49%), Gaps = 2/51 (3%)
Frame = -1
Query: 472 GTFGRWGARPGW--DRTPRPGCCRSSCSDSFDPLPSTETGTSISTACSSQS 326
GT W R G + T RPG + CS FDP G+SI+ C+ ++
Sbjct: 190 GTVQMWDPRNGGTLEETVRPGGGAAICSVEFDPF----GGSSIAVGCADRN 236
>At4g21410.1 68417.m03093 protein kinase family protein contains
Pfam PF00069: Protein kinase domain
Length = 679
Score = 29.5 bits (63), Expect = 2.7
Identities = 15/43 (34%), Positives = 23/43 (53%)
Frame = -2
Query: 483 DRLQGHLAAGGPGQVGTEHHGQVAAGHLVQTASILYLPQKLEQ 355
DRL+G AAGGP + + +G +AG+ ++ P EQ
Sbjct: 168 DRLKGIAAAGGPNRKYAQGNGSASAGYRRFYGTVQCTPDLSEQ 210
>At2g27440.1 68415.m03316 rac GTPase activating protein, putative
similar to rac GTPase activating protein 3 [Lotus
japonicus] GI:3695063; contains Pfam profiles PF00620:
RhoGAP domain, PF00786: P21-Rho-binding domain
Length = 368
Score = 29.5 bits (63), Expect = 2.7
Identities = 13/24 (54%), Positives = 17/24 (70%)
Frame = +1
Query: 376 VKDRSCLNKMTCSNLAVVFGPNLA 447
VK +NKMT NLA+VF PN++
Sbjct: 237 VKVFEVVNKMTSRNLALVFAPNMS 260
>At3g29033.1 68416.m03629 glycine-rich protein
Length = 167
Score = 28.3 bits (60), Expect = 6.3
Identities = 15/39 (38%), Positives = 19/39 (48%)
Frame = -2
Query: 522 GSPG*VGESVDWRDRLQGHLAAGGPGQVGTEHHGQVAAG 406
GS G D+ D QG+LA PG G + G V +G
Sbjct: 89 GSAQFYGYQADYADGYQGNLAGNPPGSGGYQGIGNVNSG 127
>At3g18290.1 68416.m02326 zinc finger protein-related weak alignment
to Pfam profiles: PF00097 Zinc finger, C3HC4 type (RING
finger) (2 copies)
Length = 1254
Score = 28.3 bits (60), Expect = 6.3
Identities = 14/33 (42%), Positives = 17/33 (51%)
Frame = -1
Query: 382 PLPSTETGTSISTACSSQSEVFPGSVSSHCAAS 284
PLP ET SS + V P SVSS ++S
Sbjct: 4 PLPDFETARGGGAVASSSTTVLPSSVSSSSSSS 36
>At2g46710.1 68415.m05828 rac GTPase activating protein, putative
similar to rac GTPase activating protein 2 [Lotus
japonicus] GI:3695061; contains Pfam profiles PF00620:
RhoGAP domain, PF00786: P21-Rho-binding domain
Length = 455
Score = 28.3 bits (60), Expect = 6.3
Identities = 14/46 (30%), Positives = 25/46 (54%)
Frame = +1
Query: 307 LILERLPIENYKLLKYLFQFLWKVKDRSCLNKMTCSNLAVVFGPNL 444
L++ P+E+ +L + + V + NKM N+A+VF PN+
Sbjct: 255 LVILLPPVES-AILDWAIGLMADVVEHEQFNKMNARNVAMVFAPNM 299
>At1g75800.1 68414.m08805 pathogenesis-related thaumatin family
protein similar to receptor serine/threonine kinase PR5K
[Arabidopsis thaliana] GI:1235680; contains Pfam
profile: PF00314 Thaumatin family
Length = 330
Score = 28.3 bits (60), Expect = 6.3
Identities = 15/35 (42%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = -1
Query: 427 PRPGCCRSSCSD-SFDPLPSTETGTSISTACSSQS 326
P P + S D S DP P+T TGTS +T S
Sbjct: 248 PTPNTSQKSSQDQSPDPKPTTPTGTSSTTPAGDSS 282
>At1g15780.1 68414.m01893 expressed protein
Length = 1335
Score = 28.3 bits (60), Expect = 6.3
Identities = 10/28 (35%), Positives = 20/28 (71%)
Frame = -1
Query: 379 LPSTETGTSISTACSSQSEVFPGSVSSH 296
+P+ GTSI + ++Q ++ PGS+S++
Sbjct: 103 IPAANNGTSIDSIPTNQGQLLPGSLSTN 130
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,671,831
Number of Sequences: 28952
Number of extensions: 311251
Number of successful extensions: 1012
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 968
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1012
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1833827200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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