Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0432
(857 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 110 3e-25
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 107 2e-24
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 78 2e-15
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 78 2e-15
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 55 1e-08
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 49 1e-06
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 49 1e-06
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 39 0.001
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 38 0.001
SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 33 0.069
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 32 0.12
SPAC323.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 26 7.9
SPAC227.03c |||mitochondrial NAD+ transporter|Schizosaccharomyce... 26 7.9
SPAPB1A10.05 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 26 7.9
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 110 bits (264), Expect = 3e-25
Identities = 47/83 (56%), Positives = 65/83 (78%)
Frame = +3
Query: 261 EEHRXLLSIKYPMEHGIVTDWNDMEKIWNYIYSKDQLSTFSEEHPVLLTEAPLNPRRNRE 440
+ R +L++KYP+EHGIV +W+DMEKIW++ + ++L EEHP LLTEAPLNP+ NRE
Sbjct: 59 QSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFY-NELRVAPEEHPCLLTEAPLNPKSNRE 117
Query: 441 KSAEIFFETFNVPALFLSMQAVL 509
K +I FETFN PA ++++QAVL
Sbjct: 118 KMTQIIFETFNAPAFYVAIQAVL 140
Score = 102 bits (245), Expect = 6e-23
Identities = 46/68 (67%), Positives = 56/68 (82%)
Frame = +2
Query: 509 SLYATGRTTGAVLDSGDGVTHAVPIYEGFAMPHSIMRVDVAGRDVTKYLRLLLRKEGVNL 688
SLYA+GRTTG VLDSGDGVTH VPIYEG+A+PH+IMR+D+AGRD+T YL +L + G
Sbjct: 141 SLYASGRTTGIVLDSGDGVTHTVPIYEGYALPHAIMRLDLAGRDLTDYLMKILMERGYTF 200
Query: 689 RTSAELEI 712
T+AE EI
Sbjct: 201 STTAEREI 208
Score = 61.3 bits (142), Expect = 2e-10
Identities = 26/53 (49%), Positives = 37/53 (69%)
Frame = +1
Query: 109 VVIDNGSGVIKAGFAGDQIPKCRFPNYIGRPKHTRVMAGALEGEIFVGPVQKS 267
+VIDNGSG+ KAGFAGD P+ FP+ +GRP+H +M G + + +VG +S
Sbjct: 8 LVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQS 60
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 107 bits (257), Expect = 2e-24
Identities = 49/80 (61%), Positives = 61/80 (76%)
Frame = +3
Query: 270 RXLLSIKYPMEHGIVTDWNDMEKIWNYIYSKDQLSTFSEEHPVLLTEAPLNPRRNREKSA 449
R LL I+ P+E GI+ +W+DME+IW+YIYS QL+T EEHP+LLTE PL RN+EK A
Sbjct: 66 RGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIA 125
Query: 450 EIFFETFNVPALFLSMQAVL 509
E F+ET NVPAL S+Q VL
Sbjct: 126 EYFYETLNVPALSFSLQPVL 145
Score = 88.6 bits (210), Expect = 1e-18
Identities = 40/68 (58%), Positives = 54/68 (79%)
Frame = +2
Query: 509 SLYATGRTTGAVLDSGDGVTHAVPIYEGFAMPHSIMRVDVAGRDVTKYLRLLLRKEGVNL 688
+LYA+ RTTG VL+ GDG+TH+VPIY+GF++P +I + ++ GRDVT YL+L LRK G L
Sbjct: 146 ALYASARTTGIVLECGDGLTHSVPIYDGFSIPSAIQQEEIGGRDVTDYLQLQLRKSGHEL 205
Query: 689 RTSAELEI 712
+SAE EI
Sbjct: 206 VSSAEKEI 213
Score = 81.4 bits (192), Expect = 1e-16
Identities = 34/59 (57%), Positives = 45/59 (76%)
Frame = +1
Query: 76 MELSDVIVNQPVVIDNGSGVIKAGFAGDQIPKCRFPNYIGRPKHTRVMAGALEGEIFVG 252
M ++++ NQP+ IDNGSG IKAGFAGD IPKC FP +GR KH RVM +++ ++FVG
Sbjct: 1 MTVTEIFDNQPICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVG 59
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 77.8 bits (183), Expect = 2e-15
Identities = 36/76 (47%), Positives = 49/76 (64%)
Frame = +3
Query: 282 SIKYPMEHGIVTDWNDMEKIWNYIYSKDQLSTFSEEHPVLLTEAPLNPRRNREKSAEIFF 461
S+ YP+ HG + +W+ ME+ W K L E+H LLTE PLNP NRE +AEI F
Sbjct: 89 SLDYPIRHGQIENWDHMERFWQQSLFK-YLRCEPEDHYFLLTEPPLNPPENRENTAEIMF 147
Query: 462 ETFNVPALFLSMQAVL 509
E+FN L++++QAVL
Sbjct: 148 ESFNCAGLYIAVQAVL 163
Score = 60.5 bits (140), Expect = 3e-10
Identities = 30/69 (43%), Positives = 45/69 (65%), Gaps = 1/69 (1%)
Frame = +2
Query: 467 IQCTGIISFNAGRSSLYATGRT-TGAVLDSGDGVTHAVPIYEGFAMPHSIMRVDVAGRDV 643
I +++ A +S T R+ TG V+DSGDGVTH +P+ EG+ + SI + +AGRDV
Sbjct: 157 IAVQAVLALAASWTSSKVTDRSLTGTVVDSGDGVTHIIPVAEGYVIGSSIKTMPLAGRDV 216
Query: 644 TKYLRLLLR 670
T +++ LLR
Sbjct: 217 TYFVQSLLR 225
Score = 36.7 bits (81), Expect = 0.004
Identities = 14/31 (45%), Positives = 20/31 (64%)
Frame = +1
Query: 100 NQPVVIDNGSGVIKAGFAGDQIPKCRFPNYI 192
N P+++DNG+G K G+AG+ P FP I
Sbjct: 5 NVPIIMDNGTGYSKLGYAGNDAPSYVFPTVI 35
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 77.8 bits (183), Expect = 2e-15
Identities = 35/67 (52%), Positives = 47/67 (70%)
Frame = +2
Query: 509 SLYATGRTTGAVLDSGDGVTHAVPIYEGFAMPHSIMRVDVAGRDVTKYLRLLLRKEGVNL 688
SLYA G ++G V+DSGDGVTH VP+YE + H + R+DVAGRD T+YL LL ++G
Sbjct: 141 SLYAQGLSSGVVVDSGDGVTHIVPVYESVVLNHLVGRLDVAGRDATRYLISLLLRKGYAF 200
Query: 689 RTSAELE 709
+A+ E
Sbjct: 201 NRTADFE 207
Score = 75.8 bits (178), Expect = 7e-15
Identities = 34/83 (40%), Positives = 53/83 (63%)
Frame = +3
Query: 261 EEHRXLLSIKYPMEHGIVTDWNDMEKIWNYIYSKDQLSTFSEEHPVLLTEAPLNPRRNRE 440
E R LL +KYPME+GI+ D+ +M ++W+Y + ++L +LLTE P+NP NRE
Sbjct: 59 EAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFF-EKLKIDPRGRKILLTEPPMNPVANRE 117
Query: 441 KSAEIFFETFNVPALFLSMQAVL 509
K E FE + +++++QAVL
Sbjct: 118 KMCETMFERYGFGGVYVAIQAVL 140
Score = 47.2 bits (107), Expect = 3e-06
Identities = 16/32 (50%), Positives = 25/32 (78%)
Frame = +1
Query: 106 PVVIDNGSGVIKAGFAGDQIPKCRFPNYIGRP 201
P+V+DNG+G +K G+A D P+ +FP+ +GRP
Sbjct: 5 PIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRP 36
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 54.8 bits (126), Expect = 1e-08
Identities = 28/74 (37%), Positives = 41/74 (55%)
Frame = +3
Query: 279 LSIKYPMEHGIVTDWNDMEKIWNYIYSKDQLSTFSEEHPVLLTEAPLNPRRNREKSAEIF 458
+ IK + +G V +W+ +W Y + QL T EHP+L+TE NP NR K+ E
Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQ-QLKTNPLEHPILITEPFDNPPENRVKTLETM 121
Query: 459 FETFNVPALFLSMQ 500
FE+ PA +L+ Q
Sbjct: 122 FESLRCPATYLAKQ 135
Score = 37.9 bits (84), Expect = 0.002
Identities = 17/39 (43%), Positives = 23/39 (58%)
Frame = +1
Query: 109 VVIDNGSGVIKAGFAGDQIPKCRFPNYIGRPKHTRVMAG 225
+VID GS + GF+G+ IPKC P+Y G R + G
Sbjct: 14 IVIDPGSKWTRIGFSGEDIPKCVLPSYCGEFSDGRRLFG 52
Score = 27.5 bits (58), Expect = 2.6
Identities = 20/123 (16%), Positives = 43/123 (34%)
Frame = +2
Query: 392 PSAVDRSTSEPSQE*RKVRRNIL*DIQCTGIISFNAGRSSLYATGRTTGAVLDSGDGVTH 571
P + P + K + ++C + +A+G+ T ++D G +
Sbjct: 100 PILITEPFDNPPENRVKTLETMFESLRCPATYLAKQETCAAFASGKGTACLVDIGAERSS 159
Query: 572 AVPIYEGFAMPHSIMRVDVAGRDVTKYLRLLLRKEGVNLRTSAELEIRQVYQGAGRAXCL 751
IY+GF + +G + L LR + + ++ + + A C
Sbjct: 160 VSAIYDGFVLQKGYQVQHFSGNAINDILAQTLRDKNFEVMPKYLVKSKNPVEIGQPANCE 219
Query: 752 PKP 760
+P
Sbjct: 220 LRP 222
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 48.8 bits (111), Expect = 1e-06
Identities = 25/78 (32%), Positives = 41/78 (52%)
Frame = +3
Query: 279 LSIKYPMEHGIVTDWNDMEKIWNYIYSKDQLSTFSEEHPVLLTEAPLNPRRNREKSAEIF 458
+ +K +GI+ DW W K++L E+ +++TE NP+ R++ E
Sbjct: 66 MEVKNGKSNGIIQDWESTLYTWERGL-KEKLQVNPTEYAMMITEPSWNPQSVRQQIMEAA 124
Query: 459 FETFNVPALFLSMQAVLV 512
FE +VPA +L+ QAV V
Sbjct: 125 FEQLHVPAFYLTKQAVCV 142
Score = 27.9 bits (59), Expect = 2.0
Identities = 12/29 (41%), Positives = 18/29 (62%)
Frame = +1
Query: 109 VVIDNGSGVIKAGFAGDQIPKCRFPNYIG 195
+VID GS + G+AG++ P P+Y G
Sbjct: 13 LVIDPGSCWTRFGYAGEESPMTILPSYYG 41
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 48.8 bits (111), Expect = 1e-06
Identities = 21/64 (32%), Positives = 36/64 (56%)
Frame = +3
Query: 294 PMEHGIVTDWNDMEKIWNYIYSKDQLSTFSEEHPVLLTEAPLNPRRNREKSAEIFFETFN 473
P E ++++W+ ME++ +Y + K L EHP+ +TE NP R E+ FE +N
Sbjct: 86 PFERNVISNWDLMEQVLDYTFLK--LGIDRMEHPICMTEPLANPTYVRSTMTELLFELYN 143
Query: 474 VPAL 485
P++
Sbjct: 144 APSV 147
Score = 36.7 bits (81), Expect = 0.004
Identities = 15/31 (48%), Positives = 22/31 (70%)
Frame = +1
Query: 106 PVVIDNGSGVIKAGFAGDQIPKCRFPNYIGR 198
P+VIDNGS ++AG+ G++ PK F N + R
Sbjct: 26 PLVIDNGSWQLRAGWGGEKDPKLVFDNLVSR 56
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 38.7 bits (86), Expect = 0.001
Identities = 21/75 (28%), Positives = 37/75 (49%), Gaps = 3/75 (4%)
Frame = +3
Query: 294 PMEHGIVTDWNDMEKIWNYIYS---KDQLSTFSEEHPVLLTEAPLNPRRNREKSAEIFFE 464
P++ G V DW ++ W ++YS KD T + +PV L +RE + + FFE
Sbjct: 113 PIQRGRVVDWEALKAFWKHLYSLLLKDPNDT-TFRYPVCLVIPTYWSLYDRELATQFFFE 171
Query: 465 TFNVPALFLSMQAVL 509
VP ++ + ++
Sbjct: 172 ECQVPGFTIAYEPLM 186
Score = 37.1 bits (82), Expect = 0.003
Identities = 21/64 (32%), Positives = 36/64 (56%)
Frame = +2
Query: 512 LYATGRTTGAVLDSGDGVTHAVPIYEGFAMPHSIMRVDVAGRDVTKYLRLLLRKEGVNLR 691
LYA G G V+D G T PI +G + + ++ + GR +T++L+ LLR+ L+
Sbjct: 188 LYAIGILHGLVIDIGYEKTDITPILDGQIIFTATQQLPLGGRYMTQHLQNLLRESLPTLK 247
Query: 692 TSAE 703
+S +
Sbjct: 248 SSGQ 251
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 38.3 bits (85), Expect = 0.001
Identities = 16/49 (32%), Positives = 28/49 (57%)
Frame = +2
Query: 542 VLDSGDGVTHAVPIYEGFAMPHSIMRVDVAGRDVTKYLRLLLRKEGVNL 688
V+DSG TH +P+ + ++ RVDV G+ +T YL+ ++ N+
Sbjct: 160 VIDSGYSFTHIIPVIDFSVQEQAVKRVDVGGKLLTNYLKEVISYRKYNM 208
Score = 30.7 bits (66), Expect = 0.28
Identities = 15/32 (46%), Positives = 21/32 (65%)
Frame = +1
Query: 109 VVIDNGSGVIKAGFAGDQIPKCRFPNYIGRPK 204
+V+DNG+ IKAGFAG ++ PN + R K
Sbjct: 8 IVLDNGAYHIKAGFAGGKV--VEIPNCLTRSK 37
>SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 32.7 bits (71), Expect = 0.069
Identities = 15/53 (28%), Positives = 30/53 (56%), Gaps = 1/53 (1%)
Frame = +2
Query: 515 YATGRTTGAVLDSGDGVTHAVPIYEGFAMPHSIMRVDVAGRDVT-KYLRLLLR 670
+ G + V+D G T + EG +P+S ++++ G D+T +++LL+R
Sbjct: 286 FGAGMSAACVVDMGAQKTSISCVEEGVVVPNSRIKINYGGDDITLLFMKLLMR 338
>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1666
Score = 31.9 bits (69), Expect = 0.12
Identities = 17/48 (35%), Positives = 28/48 (58%), Gaps = 2/48 (4%)
Frame = +3
Query: 369 LSTFSEEHPVLLTE--APLNPRRNREKSAEIFFETFNVPALFLSMQAV 506
L+ + E+HP+LLT+ A L PR + + IF ++ N P + M A+
Sbjct: 1403 LNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAI 1450
>SPAC323.03c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 575
Score = 25.8 bits (54), Expect = 7.9
Identities = 10/36 (27%), Positives = 17/36 (47%)
Frame = -3
Query: 219 HHSCVLRTTNIVRETTFRYLITSEARLYYAGAVVYY 112
H C+ T + + + ++ S RLYY + YY
Sbjct: 447 HARCLFLLTKTLNLDSLKSILCSRVRLYYNIEIAYY 482
>SPAC227.03c |||mitochondrial NAD+ transporter|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 371
Score = 25.8 bits (54), Expect = 7.9
Identities = 10/21 (47%), Positives = 13/21 (61%)
Frame = -1
Query: 596 RTPRIWGRRASLHRRSPVQHP 534
R PR W +R + + SP QHP
Sbjct: 197 RMPRFWLKRRTNVKSSPSQHP 217
>SPAPB1A10.05 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 285
Score = 25.8 bits (54), Expect = 7.9
Identities = 16/48 (33%), Positives = 22/48 (45%)
Frame = -2
Query: 775 SLPXRGFRKTXSTPXSLIDLTNLQLGGRPEVHSLFAE*KSKVLGDVTP 632
S+P + T +TP + N L RP +HS K L D+TP
Sbjct: 3 SVPLQNENST-TTPTKEVSHLNFPLK-RPRLHSFVPTSKQAALSDITP 48
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,404,123
Number of Sequences: 5004
Number of extensions: 71315
Number of successful extensions: 224
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 197
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 218
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 426466470
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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