Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0431
(516 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3 |Sch... 36 0.005
SPBC13E7.02 |cwf24||GCN5-related N acetyltransferase|Schizosacch... 35 0.008
SPAC6B12.07c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 34 0.015
SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 33 0.019
SPAC16C9.04c |||CCR4-Not complex subunit Mot2 |Schizosaccharomyc... 31 0.077
SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 30 0.18
SPAC17G6.08 |pep7|vac1|prevacuole/endosomal FYVE tethering compo... 29 0.31
SPBC1734.06 |rhp18||Rad18 homolog Rhp18|Schizosaccharomyces pomb... 29 0.41
SPBC23E6.02 |||ATP-dependent DNA helicase |Schizosaccharomyces p... 29 0.41
SPBC29A3.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 29 0.41
SPBC12C2.10c |pst1|SPBC21D10.01c|Clr6 histone deacetylase comple... 28 0.72
SPAC19A8.05c |vps27|sst4|sorting receptor for ubiquitinated memb... 28 0.95
SPBC17D11.02c |||synoviolin homolog|Schizosaccharomyces pombe|ch... 26 2.9
SPAC16E8.13 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 26 2.9
SPCC306.06c |||ER membrane protein, BIG1 family |Schizosaccharom... 26 3.8
SPBC2F12.05c |||sterol binding ankyrin repeat protein|Schizosacc... 26 3.8
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 26 3.8
SPAC1002.14 |itt1||ubiquitin-protein ligase E3 |Schizosaccharomy... 25 5.1
SPBC2D10.14c |myo51||myosin type V|Schizosaccharomyces pombe|chr... 25 6.7
SPBP8B7.23 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p... 25 6.7
SPCC970.10c |brl2|rfp1|ubiquitin-protein ligase E3 Brl2|Schizosa... 25 8.9
SPBC3E7.01 |fab1|ste12, SPBC6B1.11c|1-phosphatidylinositol-3-pho... 25 8.9
SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc... 25 8.9
SPAC23A1.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 25 8.9
SPAC144.05 |||ATP-dependent DNA helicase|Schizosaccharomyces pom... 25 8.9
>SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1133
Score = 35.5 bits (78), Expect = 0.005
Identities = 13/29 (44%), Positives = 18/29 (62%)
Frame = +1
Query: 121 CPVCCREFGAPPRSPISLSCGHTLCRHCL 207
CP+CC E P ++P+ L+C H C CL
Sbjct: 877 CPICCNE---PIQNPLLLNCKHACCGDCL 902
>SPBC13E7.02 |cwf24||GCN5-related N
acetyltransferase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 533
Score = 34.7 bits (76), Expect = 0.008
Identities = 14/35 (40%), Positives = 21/35 (60%)
Frame = +1
Query: 121 CPVCCREFGAPPRSPISLSCGHTLCRHCLIHLHRK 225
C +C +++ RSPI+ +CGH C C I +RK
Sbjct: 254 CLICKKDY----RSPIATTCGHHFCEQCAITRYRK 284
>SPAC6B12.07c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 456
Score = 33.9 bits (74), Expect = 0.015
Identities = 16/47 (34%), Positives = 22/47 (46%)
Frame = +1
Query: 97 PQWTDYLTCPVCCREFGAPPRSPISLSCGHTLCRHCLIHLHRKQCPF 237
PQ D+ C +C P+ L C H C HCLI L +++ F
Sbjct: 353 PQLRDF-ECAICSNV----AYKPVRLGCSHVFCLHCLIILQKQKVDF 394
>SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 468
Score = 33.5 bits (73), Expect = 0.019
Identities = 15/42 (35%), Positives = 20/42 (47%), Gaps = 2/42 (4%)
Frame = +1
Query: 115 LTCPVCCREFGAPPRSPISLSCGHTLCRHCLIH--LHRKQCP 234
L CP+C + P + CGHT C CL++ K CP
Sbjct: 83 LECPICTEAL----QRPFTTHCGHTYCYECLLNWLKESKSCP 120
>SPAC16C9.04c |||CCR4-Not complex subunit Mot2 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 489
Score = 31.5 bits (68), Expect = 0.077
Identities = 11/36 (30%), Positives = 18/36 (50%)
Frame = +1
Query: 109 DYLTCPVCCREFGAPPRSPISLSCGHTLCRHCLIHL 216
D + CP+C E ++ CG+ +CR C H+
Sbjct: 14 DDMCCPLCMEEIDISDKNFKPCQCGYRVCRFCWHHI 49
>SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 486
Score = 30.3 bits (65), Expect = 0.18
Identities = 13/31 (41%), Positives = 16/31 (51%)
Frame = +1
Query: 112 YLTCPVCCREFGAPPRSPISLSCGHTLCRHC 204
Y CP C P P++L CG T+CR C
Sbjct: 16 YFICPGC----NCLPDWPVTLPCGGTVCRKC 42
Score = 28.3 bits (60), Expect = 0.72
Identities = 16/42 (38%), Positives = 18/42 (42%), Gaps = 2/42 (4%)
Frame = +1
Query: 115 LTCPVCCREFGAPPRSPISLSCGHTLCRHCLIH--LHRKQCP 234
L C +C P SP CGHT C CL+ QCP
Sbjct: 167 LECQICFGMLYDPVVSP----CGHTFCGPCLMQALTQSPQCP 204
>SPAC17G6.08 |pep7|vac1|prevacuole/endosomal FYVE tethering
component Pep7 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 536
Score = 29.5 bits (63), Expect = 0.31
Identities = 12/38 (31%), Positives = 16/38 (42%)
Frame = +1
Query: 121 CPVCCREFGAPPRSPISLSCGHTLCRHCLIHLHRKQCP 234
CP C F R CG +CR C++ + Q P
Sbjct: 281 CPECNNSFTLTRRRRHCRLCGRVICRFCVLEISLPQHP 318
>SPBC1734.06 |rhp18||Rad18 homolog Rhp18|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 387
Score = 29.1 bits (62), Expect = 0.41
Identities = 15/42 (35%), Positives = 20/42 (47%), Gaps = 2/42 (4%)
Frame = +1
Query: 115 LTCPVCCREFGAPPRSPISLSCGHTLCRHCLIHLHRK--QCP 234
L C +C F R+P+ SC HT C C+ R+ CP
Sbjct: 27 LRCLICHEYF----RAPLITSCSHTFCSFCIRDYLREHPMCP 64
>SPBC23E6.02 |||ATP-dependent DNA helicase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1040
Score = 29.1 bits (62), Expect = 0.41
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = +1
Query: 166 ISLSCGHTLCRHCLIHL 216
I + CGH LCR CL H+
Sbjct: 758 IIVPCGHFLCRECLTHV 774
>SPBC29A3.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 398
Score = 29.1 bits (62), Expect = 0.41
Identities = 15/45 (33%), Positives = 22/45 (48%), Gaps = 5/45 (11%)
Frame = +1
Query: 118 TCPVCCREFGAPPRSPISLSCGHTLCRHCLIHLHRK-----QCPF 237
TCPV +E P+ +SCGH + + L L R +CP+
Sbjct: 340 TCPVS-KEQATEENPPMMMSCGHVIVKESLRQLSRNGSQRFKCPY 383
>SPBC12C2.10c |pst1|SPBC21D10.01c|Clr6 histone deacetylase complex
subunit Pst1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1522
Score = 28.3 bits (60), Expect = 0.72
Identities = 28/116 (24%), Positives = 49/116 (42%), Gaps = 7/116 (6%)
Frame = +3
Query: 66 RVIYKNANSGASVDGLPNLSGVLPRVRCSAPESYQPQLRP--HP-L*ALSDSPSS--ETM 230
R++Y+N N+ S +PN + V P P +P HP + +S SP+ E
Sbjct: 78 RLLYRNVNTQVSHTAIPNPANGYAAVYGGPPSQLPPPPQPQSHPNVTVISASPARAIEQQ 137
Query: 231 P--LRPDDHTVEPEELVVNTALLQLAGYAPPSQPYQPPCIKALPESDRQAYDTVIR 392
P L D + V ++A + P + P P + L +D +Y +++
Sbjct: 138 PTILSSTDSNIPRPGTVKSSASPFVPNQNPSAPPPPPQEYRQLNVTDALSYLDLVK 193
>SPAC19A8.05c |vps27|sst4|sorting receptor for ubiquitinated
membrane proteins, ESCRT 0 complex|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 610
Score = 27.9 bits (59), Expect = 0.95
Identities = 12/36 (33%), Positives = 13/36 (36%)
Frame = +1
Query: 97 PQWTDYLTCPVCCREFGAPPRSPISLSCGHTLCRHC 204
P WTD C C F R +CG C C
Sbjct: 168 PDWTDSEVCLRCRTPFTFTNRKHHCRNCGGVFCNQC 203
>SPBC17D11.02c |||synoviolin homolog|Schizosaccharomyces pombe|chr
2|||Manual
Length = 677
Score = 26.2 bits (55), Expect = 2.9
Identities = 14/27 (51%), Positives = 17/27 (62%), Gaps = 2/27 (7%)
Frame = +1
Query: 160 SPISLSCGHTLCRHCLIH-LHRKQ-CP 234
+P L CGH L HCL + L R+Q CP
Sbjct: 322 TPKRLPCGHILHFHCLRNWLERQQTCP 348
>SPAC16E8.13 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 547
Score = 26.2 bits (55), Expect = 2.9
Identities = 13/46 (28%), Positives = 17/46 (36%)
Frame = +1
Query: 97 PQWTDYLTCPVCCREFGAPPRSPISLSCGHTLCRHCLIHLHRKQCP 234
P + TC VC + I++ C HT CL CP
Sbjct: 200 PALIELPTCVVCLERMDSSITGLITIVCQHTFHCPCLQKWGNSSCP 245
>SPCC306.06c |||ER membrane protein, BIG1 family
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 311
Score = 25.8 bits (54), Expect = 3.8
Identities = 15/41 (36%), Positives = 19/41 (46%)
Frame = -2
Query: 485 LPLHRPRKSIXXATHIGVSGAFQEYSKFLHGAYNSVVRLPV 363
L +H+ RK T G +G F EY F G Y + L V
Sbjct: 243 LAMHQTRKH--PVTDNGATGLFTEYQFFTPGLYMGYLALAV 281
>SPBC2F12.05c |||sterol binding ankyrin repeat
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1310
Score = 25.8 bits (54), Expect = 3.8
Identities = 10/25 (40%), Positives = 12/25 (48%)
Frame = +2
Query: 347 GSPGVGQAGVRHCYTHHGGTCCILE 421
GSP V G H H G C+L+
Sbjct: 1108 GSPSVDNYGQMHIVNHSSGINCVLD 1132
>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1224
Score = 25.8 bits (54), Expect = 3.8
Identities = 16/65 (24%), Positives = 25/65 (38%), Gaps = 1/65 (1%)
Frame = +3
Query: 186 HPL*ALSDSPSSETMPLRPDDHT-VEPEELVVNTALLQLAGYAPPSQPYQPPCIKALPES 362
HP A P P+ +T V P+ V + + + PPS PY P + + +
Sbjct: 936 HPPTASGTRVPPVQQPSHPNPYTPVAPQSPVAAASRISSSPNMPPSNPYTPIAVASSTVN 995
Query: 363 DRQAY 377
Y
Sbjct: 996 PAHTY 1000
>SPAC1002.14 |itt1||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 435
Score = 25.4 bits (53), Expect = 5.1
Identities = 11/29 (37%), Positives = 14/29 (48%)
Frame = +1
Query: 121 CPVCCREFGAPPRSPISLSCGHTLCRHCL 207
C VC EF ++ CGH C+ CL
Sbjct: 174 CNVCFDEFNGTDCFQLT-RCGHVSCQSCL 201
>SPBC2D10.14c |myo51||myosin type V|Schizosaccharomyces pombe|chr
2|||Manual
Length = 1471
Score = 25.0 bits (52), Expect = 6.7
Identities = 16/38 (42%), Positives = 22/38 (57%), Gaps = 2/38 (5%)
Frame = -3
Query: 448 PLILAFPEHFKNTASS--SMVRITVSYACLSDSGRALI 341
PLIL F +HF N +S S+V+ +S C S AL+
Sbjct: 1212 PLILKFSKHFSNLENSFHSLVQKLLS-CCTEGSINALL 1248
>SPBP8B7.23 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 673
Score = 25.0 bits (52), Expect = 6.7
Identities = 12/30 (40%), Positives = 15/30 (50%)
Frame = +1
Query: 121 CPVCCREFGAPPRSPISLSCGHTLCRHCLI 210
CP C E P + +S CGH C CL+
Sbjct: 214 CPFCLEE--KPVAARMS-RCGHVYCFSCLL 240
>SPCC970.10c |brl2|rfp1|ubiquitin-protein ligase E3
Brl2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 680
Score = 24.6 bits (51), Expect = 8.9
Identities = 10/18 (55%), Positives = 11/18 (61%)
Frame = +1
Query: 121 CPVCCREFGAPPRSPISL 174
CP+C R FGA PI L
Sbjct: 663 CPICGRGFGASDVIPIHL 680
>SPBC3E7.01 |fab1|ste12,
SPBC6B1.11c|1-phosphatidylinositol-3-phosphate 5-kinase
Fab1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 1932
Score = 24.6 bits (51), Expect = 8.9
Identities = 10/29 (34%), Positives = 14/29 (48%)
Frame = +1
Query: 121 CPVCCREFGAPPRSPISLSCGHTLCRHCL 207
C +C EF R CG +C++CL
Sbjct: 66 CSLCETEFTLFRRKHHCRICGKIICKYCL 94
>SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme
Ubc6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 227
Score = 24.6 bits (51), Expect = 8.9
Identities = 8/19 (42%), Positives = 12/19 (63%)
Frame = +3
Query: 312 PPSQPYQPPCIKALPESDR 368
PP P++PP I+ + S R
Sbjct: 61 PPDYPFKPPAIRMITPSGR 79
>SPAC23A1.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 251
Score = 24.6 bits (51), Expect = 8.9
Identities = 7/17 (41%), Positives = 11/17 (64%)
Frame = +1
Query: 160 SPISLSCGHTLCRHCLI 210
+P + CGHT C C++
Sbjct: 207 TPYTTDCGHTYCYACIM 223
>SPAC144.05 |||ATP-dependent DNA helicase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1375
Score = 24.6 bits (51), Expect = 8.9
Identities = 11/31 (35%), Positives = 14/31 (45%), Gaps = 2/31 (6%)
Frame = +1
Query: 175 SCGHTLCRHCLIH--LHRKQCPFDQTIIQLN 261
+CGH C CL H CP +T + N
Sbjct: 1106 TCGHLYCSFCLEAWLKHSSSCPMCKTKLNKN 1136
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,070,745
Number of Sequences: 5004
Number of extensions: 42222
Number of successful extensions: 171
Number of sequences better than 10.0: 25
Number of HSP's better than 10.0 without gapping: 161
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 170
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 208287218
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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