Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0423
(755 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr ... 38 0.002
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 37 0.003
SPAC1002.18 |urg3||DUF1688 family protein|Schizosaccharomyces po... 30 0.31
SPBC21.07c |ppk24||serine/threonine protein kinase Ppk24|Schizos... 30 0.31
SPBC29A3.04 |rpl8||60S ribosomal protein L7a |Schizosaccharomyce... 30 0.41
SPAC22G7.08 |ppk8||serine/threonine protein kinase Ppk8 |Schizos... 28 1.7
SPAC23E2.02 |lsd2|swm2, saf140|histone demethylase SWIRM2 |Schiz... 27 2.9
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 27 3.8
SPBC577.06c |||phosphatidylinositol kinase |Schizosaccharomyces ... 26 5.0
SPBC12C2.06 |||ATP-dependent RNA helicase Dbp5|Schizosaccharomyc... 26 5.0
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 26 6.7
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 26 6.7
SPAC167.03c |snu66||U4/U6 x U5 tri-snRNP complex subunit Snu66 |... 25 8.8
>SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 621
Score = 37.5 bits (83), Expect = 0.002
Identities = 22/60 (36%), Positives = 33/60 (55%), Gaps = 2/60 (3%)
Frame = +3
Query: 324 LEQQIQARNHSGVSEDALKEFSMMFKHFDKDRSGRLNHHEFKSCLRALG--YDLPMVEEG 497
LE ++ R +S+ L + +F+HF+K +S LN EF + L +LG YD EEG
Sbjct: 469 LENELLEREKRTLSKQELDGITKVFRHFEKKKSNMLNEVEFYAALASLGLVYD---TEEG 525
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 37.1 bits (82), Expect = 0.003
Identities = 12/39 (30%), Positives = 27/39 (69%)
Frame = +3
Query: 360 VSEDALKEFSMMFKHFDKDRSGRLNHHEFKSCLRALGYD 476
++E+ ++ + FK FD D+ +++HE ++ +RALG++
Sbjct: 31 ITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFN 69
>SPAC1002.18 |urg3||DUF1688 family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 399
Score = 30.3 bits (65), Expect = 0.31
Identities = 27/99 (27%), Positives = 48/99 (48%), Gaps = 2/99 (2%)
Frame = -2
Query: 514 KLGSGCPSSTMGRSYPRAR-RHDLNSWWLRRPDRSLSKCLNIIENSFRASSLTPEWFLAC 338
+LGSG PS +GR +P LN L ++L + + SF +L W+L+
Sbjct: 198 QLGSGRPSDFVGRIFPTGDFSKGLNVLELWSELQTLLIPIWPVRTSFEGQNLGDAWYLST 257
Query: 337 ICCSRLCCMRIESWSSWSHCALVP-RCCARVAVVQDQML 224
I + ++ W ++S L+P + +V VV +++L
Sbjct: 258 IDAIQ-PFHKLTQWLTYS--LLIPFKSLLKVPVVNEELL 293
>SPBC21.07c |ppk24||serine/threonine protein kinase
Ppk24|Schizosaccharomyces pombe|chr 2|||Manual
Length = 461
Score = 30.3 bits (65), Expect = 0.31
Identities = 13/28 (46%), Positives = 18/28 (64%)
Frame = +1
Query: 415 IDLDASTTTSSSRVCEPSGTTCPWLKRD 498
I++ STT SS V +PS T PWL+ +
Sbjct: 48 INVSKSTTKKSSVVIKPSTITAPWLENE 75
>SPBC29A3.04 |rpl8||60S ribosomal protein L7a |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 259
Score = 29.9 bits (64), Expect = 0.41
Identities = 15/45 (33%), Positives = 24/45 (53%)
Frame = +2
Query: 317 AQPRAADTSQEPFRCKRRRPEGVLDDVQALRQGSIWTPQPPRVQV 451
AQP+AA T++ P R R G+ D+Q R S + P +++
Sbjct: 14 AQPKAAKTTKNPLFVSRPRSFGIGQDIQPKRDLSRFVKWPEYIRL 58
>SPAC22G7.08 |ppk8||serine/threonine protein kinase Ppk8
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 513
Score = 27.9 bits (59), Expect = 1.7
Identities = 17/51 (33%), Positives = 27/51 (52%), Gaps = 2/51 (3%)
Frame = +2
Query: 2 SKEAQRQEENDKL--RKEFAKHANTFHXWLTETRTSMMEGTGSLEQQLATL 148
S E++ +++D L + +T H L+ T TSM E + S E + ATL
Sbjct: 69 SIESEESDKSDYLLDHAHSLQELSTTHSSLSSTLTSMSEESSSTESKFATL 119
>SPAC23E2.02 |lsd2|swm2, saf140|histone demethylase SWIRM2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1273
Score = 27.1 bits (57), Expect = 2.9
Identities = 14/46 (30%), Positives = 24/46 (52%), Gaps = 1/46 (2%)
Frame = -1
Query: 197 GVSGQLCGRVLRTLVGAASPTVVPGNPCPPS-LMCESR*AXGGRCW 63
G++G +C R L L S + + N PP ++ E++ GGR +
Sbjct: 515 GLTGLICARQLTGLFSQYSSSFLSKNELPPKVIILEAKERTGGRIY 560
>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 190
Score = 26.6 bits (56), Expect = 3.8
Identities = 9/26 (34%), Positives = 17/26 (65%)
Frame = +2
Query: 497 TTRSEFESILSIVDPNRDGQVSLQEY 574
T I +++D N+DGQ++L+E+
Sbjct: 144 TPEKRVNKIFNMMDKNKDGQLTLEEF 169
>SPBC577.06c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1877
Score = 26.2 bits (55), Expect = 5.0
Identities = 12/38 (31%), Positives = 22/38 (57%), Gaps = 2/38 (5%)
Frame = -2
Query: 415 SLSKCLNIIENSFRASSLTPEWF--LACICCSRLCCMR 308
+L+ + + R +S T + F L C+CC+R+ C+R
Sbjct: 766 NLTSFVESLSKERRVASSTIDEFQKLLCLCCNRVDCVR 803
>SPBC12C2.06 |||ATP-dependent RNA helicase Dbp5|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 503
Score = 26.2 bits (55), Expect = 5.0
Identities = 8/22 (36%), Positives = 15/22 (68%)
Frame = +3
Query: 465 LGYDLPMVEEGQPDPSLNLYSV 530
+ YD+P+ + G+PDP L+ +
Sbjct: 425 VNYDMPLDQAGRPDPQTYLHRI 446
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 25.8 bits (54), Expect = 6.7
Identities = 18/72 (25%), Positives = 33/72 (45%)
Frame = +2
Query: 383 VLDDVQALRQGSIWTPQPPRVQVVSASPRVRPAHG*RGTTRSEFESILSIVDPNRDGQVS 562
V++++ + GS++ P+ + A+G R FE I+ ++D DG S
Sbjct: 73 VVNNILSDTYGSLYNPENILITKNGGGAGNNWANGYSHAERI-FEDIMDMIDREADGSDS 131
Query: 563 LQEYMGLHDQQG 598
L+ + LH G
Sbjct: 132 LEGFSLLHSIAG 143
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 25.8 bits (54), Expect = 6.7
Identities = 13/34 (38%), Positives = 19/34 (55%)
Frame = +3
Query: 378 KEFSMMFKHFDKDRSGRLNHHEFKSCLRALGYDL 479
+EF F+ FDKD +G + E + L +LG L
Sbjct: 77 EEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKL 110
>SPAC167.03c |snu66||U4/U6 x U5 tri-snRNP complex subunit Snu66
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 649
Score = 25.4 bits (53), Expect = 8.8
Identities = 12/41 (29%), Positives = 20/41 (48%)
Frame = +3
Query: 594 KETENVHSSEEIENAFRAITAHQDRPLRHQGRTVTRTSRRE 716
+E N EE+++ F + +R R QG+T+ T E
Sbjct: 64 QEEINRKKEEELKSKFEKLRQKNERRRRTQGKTLAETLAEE 104
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,890,612
Number of Sequences: 5004
Number of extensions: 57961
Number of successful extensions: 199
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 194
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 199
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 361294920
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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