Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0409
(853 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g18720.1 68415.m02180 eukaryotic translation initiation facto... 134 7e-32
At4g18330.2 68417.m02719 eukaryotic translation initiation facto... 132 4e-31
At4g18330.1 68417.m02718 eukaryotic translation initiation facto... 132 4e-31
At1g04170.1 68414.m00407 eukaryotic translation initiation facto... 131 5e-31
At5g66470.1 68418.m08382 expressed protein 37 0.020
At4g11150.1 68417.m01807 vacuolar ATP synthase subunit E / V-ATP... 31 0.97
At5g13280.1 68418.m01525 aspartate kinase identical to aspartate... 29 3.9
At3g08560.1 68416.m00993 vacuolar ATP synthase subunit E, putati... 29 3.9
At5g28740.1 68418.m03530 transcription-coupled DNA repair protei... 29 5.2
At5g14060.1 68418.m01645 aspartate kinase, lysine-sensitive near... 29 5.2
At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,... 29 5.2
At3g02020.1 68416.m00164 aspartate kinase, lysine-sensitive, put... 29 5.2
At2g36360.1 68415.m04462 kelch repeat-containing protein low sim... 28 6.9
At1g64200.1 68414.m07273 vacuolar ATP synthase subunit E, putati... 28 6.9
At5g46370.1 68418.m05707 outward rectifying potassium channel, p... 28 9.1
At4g16155.1 68417.m02451 dihydrolipoamide dehydrogenase 2, plast... 28 9.1
At1g44740.1 68414.m05125 expressed protein ; expression supporte... 28 9.1
>At2g18720.1 68415.m02180 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative
Length = 465
Score = 134 bits (324), Expect = 7e-32
Identities = 60/83 (72%), Positives = 73/83 (87%)
Frame = +3
Query: 6 LKHILILQNKIDLVKEGQAKEQHEQIVKFVQGTVAEGAPIIPISAQLKYNIEVLCEYITK 185
LK I+I+QNKIDL++E +A +QHE I +F+ T AEGAPI+P+SAQLKYNI+VLCEYI K
Sbjct: 173 LKDIIIIQNKIDLIQENEAIKQHEDIQRFITNTNAEGAPIVPVSAQLKYNIDVLCEYIVK 232
Query: 186 KIPVPLRDFTSPPRMIVIRSFDV 254
KIP+P+RDF SPPRMIVIRSFDV
Sbjct: 233 KIPLPVRDFVSPPRMIVIRSFDV 255
Score = 126 bits (303), Expect = 2e-29
Identities = 56/85 (65%), Positives = 69/85 (81%)
Frame = +2
Query: 254 NKPGCEVDDLRGGVAGGSILQGVLTVGMEIEVRPGLVSKDADGKLTCRPIFSRIVSLFAE 433
NKPG + D ++GGVAGGSILQGVL VG IE+RPG+ KD +G C PI++RI SL+AE
Sbjct: 256 NKPGSDYDGMKGGVAGGSILQGVLKVGHIIEIRPGITRKDENGDTKCTPIYTRITSLYAE 315
Query: 434 QNELQYAVPGGLIGVGTKIEPTLCR 508
QNELQ+AVPGGLIG+GT ++PTL R
Sbjct: 316 QNELQFAVPGGLIGIGTSMDPTLTR 340
Score = 103 bits (248), Expect = 1e-22
Identities = 55/100 (55%), Positives = 71/100 (71%)
Frame = +1
Query: 511 DRLVGQVLGAVGCLPGIFVKLEVSYYLLKRLLGVRTEGDKKAAKVQKLVKNEVLLVNIGS 690
DRLVGQVLG +G LP ++V+LEVS+ LL RL+GV+T+ ++ +V KLVK E+L++NIGS
Sbjct: 342 DRLVGQVLGEIGTLPDVYVELEVSFQLLTRLIGVKTKETERQMRVSKLVKGEILMLNIGS 401
Query: 691 LSTGGRVIATKVDFG*KSPFTNPCCTGIGEKS*HWSRRVE 810
+STG VI K D K T P CT IGEK SRRV+
Sbjct: 402 MSTGAMVIGVKKDMV-KVNLTLPVCTSIGEKV-AISRRVD 439
>At4g18330.2 68417.m02719 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 471
Score = 132 bits (318), Expect = 4e-31
Identities = 60/84 (71%), Positives = 71/84 (84%)
Frame = +3
Query: 3 KLKHILILQNKIDLVKEGQAKEQHEQIVKFVQGTVAEGAPIIPISAQLKYNIEVLCEYIT 182
+LKHI+ILQNKIDL+ E A EQHE I KF+ T AE API+P+SAQLKYNI+V+ EYI
Sbjct: 180 RLKHIIILQNKIDLINEKAATEQHEAIQKFITNTNAEDAPIVPVSAQLKYNIDVVSEYIV 239
Query: 183 KKIPVPLRDFTSPPRMIVIRSFDV 254
KKIP+P+RDF SPP+MIVIRSFDV
Sbjct: 240 KKIPIPVRDFVSPPKMIVIRSFDV 263
Score = 121 bits (292), Expect = 5e-28
Identities = 56/85 (65%), Positives = 66/85 (77%)
Frame = +2
Query: 254 NKPGCEVDDLRGGVAGGSILQGVLTVGMEIEVRPGLVSKDADGKLTCRPIFSRIVSLFAE 433
NKPG +++GGVAGGSILQGVL V IE+RPG+ KD G C PI+SRI SL+AE
Sbjct: 264 NKPGSAGHEMKGGVAGGSILQGVLKVNQLIEIRPGITGKDEHGNSKCTPIYSRITSLYAE 323
Query: 434 QNELQYAVPGGLIGVGTKIEPTLCR 508
QNELQ+AVPGGLIGVGT ++PTL R
Sbjct: 324 QNELQFAVPGGLIGVGTTMDPTLTR 348
Score = 112 bits (269), Expect = 3e-25
Identities = 60/101 (59%), Positives = 73/101 (72%)
Frame = +1
Query: 508 SDRLVGQVLGAVGCLPGIFVKLEVSYYLLKRLLGVRTEGDKKAAKVQKLVKNEVLLVNIG 687
+DRLVGQVLG +G LP +FV+LEVSY LL RL+GVRT+ +K KV KL K E+L+VNIG
Sbjct: 349 ADRLVGQVLGEMGTLPDVFVELEVSYQLLTRLIGVRTKEKEKQMKVSKLTKEEILMVNIG 408
Query: 688 SLSTGGRVIATKVDFG*KSPFTNPCCTGIGEKS*HWSRRVE 810
S+STG +VI K D K T+P CT IGEK SRRV+
Sbjct: 409 SMSTGAKVIGVKKDMV-KLQLTSPVCTTIGEKV-ALSRRVD 447
>At4g18330.1 68417.m02718 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 284
Score = 132 bits (318), Expect = 4e-31
Identities = 60/84 (71%), Positives = 71/84 (84%)
Frame = +3
Query: 3 KLKHILILQNKIDLVKEGQAKEQHEQIVKFVQGTVAEGAPIIPISAQLKYNIEVLCEYIT 182
+LKHI+ILQNKIDL+ E A EQHE I KF+ T AE API+P+SAQLKYNI+V+ EYI
Sbjct: 180 RLKHIIILQNKIDLINEKAATEQHEAIQKFITNTNAEDAPIVPVSAQLKYNIDVVSEYIV 239
Query: 183 KKIPVPLRDFTSPPRMIVIRSFDV 254
KKIP+P+RDF SPP+MIVIRSFDV
Sbjct: 240 KKIPIPVRDFVSPPKMIVIRSFDV 263
Score = 33.5 bits (73), Expect = 0.18
Identities = 14/21 (66%), Positives = 17/21 (80%)
Frame = +2
Query: 254 NKPGCEVDDLRGGVAGGSILQ 316
NKPG +++GGVAGGSILQ
Sbjct: 264 NKPGSAGHEMKGGVAGGSILQ 284
>At1g04170.1 68414.m00407 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to gb|U37354 from S. pombe. ESTs
gb|T41979, gb|N37284 and gb|N37529 come from this gene
Length = 465
Score = 131 bits (317), Expect = 5e-31
Identities = 59/84 (70%), Positives = 71/84 (84%)
Frame = +3
Query: 3 KLKHILILQNKIDLVKEGQAKEQHEQIVKFVQGTVAEGAPIIPISAQLKYNIEVLCEYIT 182
+LKHI+ILQNKIDL++E A QHE I KF+ TVA+ API+P+SAQLKYNI+V+CEYI
Sbjct: 174 QLKHIIILQNKIDLIQENVAINQHEAIQKFIMNTVADAAPIVPVSAQLKYNIDVVCEYIV 233
Query: 183 KKIPVPLRDFTSPPRMIVIRSFDV 254
KKIP+P R+F SPP MIVIRSFDV
Sbjct: 234 KKIPIPERNFVSPPNMIVIRSFDV 257
Score = 130 bits (314), Expect = 1e-30
Identities = 59/85 (69%), Positives = 72/85 (84%)
Frame = +2
Query: 254 NKPGCEVDDLRGGVAGGSILQGVLTVGMEIEVRPGLVSKDADGKLTCRPIFSRIVSLFAE 433
NKPG EVD+++GGVAGGSIL+GVL V IE+RPG+V+KD G C PI+SRI+SL+AE
Sbjct: 258 NKPGYEVDEIKGGVAGGSILRGVLRVNQLIEIRPGIVTKDERGNSKCTPIYSRIISLYAE 317
Query: 434 QNELQYAVPGGLIGVGTKIEPTLCR 508
QNELQ+AVPGGLIGVGT ++PTL R
Sbjct: 318 QNELQFAVPGGLIGVGTTMDPTLTR 342
Score = 118 bits (283), Expect = 7e-27
Identities = 59/101 (58%), Positives = 75/101 (74%)
Frame = +1
Query: 508 SDRLVGQVLGAVGCLPGIFVKLEVSYYLLKRLLGVRTEGDKKAAKVQKLVKNEVLLVNIG 687
+DRLVGQVLG +G LP +FV+LEV+++LL+RLLGVRT+G +K KV KL K E+L++NIG
Sbjct: 343 ADRLVGQVLGEIGSLPDVFVELEVNFFLLRRLLGVRTKGSEKQGKVSKLTKGEILMLNIG 402
Query: 688 SLSTGGRVIATKVDFG*KSPFTNPCCTGIGEKS*HWSRRVE 810
S+STG +V+ KVD K T P CT GEK SRRVE
Sbjct: 403 SMSTGAKVVGVKVDLA-KLQLTAPVCTSKGEKV-ALSRRVE 441
>At5g66470.1 68418.m08382 expressed protein
Length = 427
Score = 36.7 bits (81), Expect = 0.020
Identities = 22/60 (36%), Positives = 36/60 (60%)
Frame = +3
Query: 15 ILILQNKIDLVKEGQAKEQHEQIVKFVQGTVAEGAPIIPISAQLKYNIEVLCEYITKKIP 194
+L++ NK DL+K G+ ++ E KF V E +IP+SA+ + IE + E+I K+P
Sbjct: 243 MLLVMNKKDLIKPGEIAKKLEWYEKFTD--VDE---VIPVSAKYGHGIEDVKEWILSKLP 297
>At4g11150.1 68417.m01807 vacuolar ATP synthase subunit E / V-ATPase
E subunit / vacuolar proton pump E subunit (VATE)
identical to SP|Q39258 Vacuolar ATP synthase subunit E
(EC 3.6.3.14) (V-ATPase E subunit) (Vacuolar proton pump
E subunit) {Arabidopsis thaliana}
Length = 230
Score = 31.1 bits (67), Expect = 0.97
Identities = 14/41 (34%), Positives = 25/41 (60%)
Frame = +3
Query: 45 VKEGQAKEQHEQIVKFVQGTVAEGAPIIPISAQLKYNIEVL 167
+ +G Q +Q+V+F++ E A I +SA+ ++NIE L
Sbjct: 1 MNDGDVSRQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKL 41
>At5g13280.1 68418.m01525 aspartate kinase identical to aspartate
kinase [Arabidopsis thaliana] GI:4376158
Length = 569
Score = 29.1 bits (62), Expect = 3.9
Identities = 22/56 (39%), Positives = 28/56 (50%), Gaps = 5/56 (8%)
Frame = +2
Query: 299 GGSILQGVLTVGMEIEVRPGLVSKDADGKLTCRP-IFSRIVSL----FAEQNELQY 451
GGS L T+G + ++ V KD DG LTC P I+ R + F E EL Y
Sbjct: 290 GGSDLTAT-TIGKALGLKEIQVWKDVDGVLTCDPTIYKRATPVPYLTFDEAAELAY 344
>At3g08560.1 68416.m00993 vacuolar ATP synthase subunit E, putative
/ V-ATPase E subunit, putative / vacuolar proton pump E
subunit, putative similar to SP|Q39258 Vacuolar ATP
synthase subunit E (EC 3.6.3.14) (V-ATPase E subunit)
(Vacuolar proton pump E subunit) {Arabidopsis thaliana};
contains Pfam profile PF01991: ATP synthase (E/31 kDa)
subunit
Length = 235
Score = 29.1 bits (62), Expect = 3.9
Identities = 14/41 (34%), Positives = 25/41 (60%)
Frame = +3
Query: 45 VKEGQAKEQHEQIVKFVQGTVAEGAPIIPISAQLKYNIEVL 167
+ + +Q +Q+V+F++ E A I ISA+ ++NIE L
Sbjct: 1 MNDADVSKQIQQMVRFIRQEAEEKANEISISAEEEFNIERL 41
>At5g28740.1 68418.m03530 transcription-coupled DNA repair
protein-related similar to XAB2 (XPA-binding protein 2)
[Homo sapiens] GI:10566459
Length = 917
Score = 28.7 bits (61), Expect = 5.2
Identities = 15/38 (39%), Positives = 20/38 (52%)
Frame = +1
Query: 562 FVKLEVSYYLLKRLLGVRTEGDKKAAKVQKLVKNEVLL 675
+ KLE Y L KR + V E KK + QKL E+ +
Sbjct: 630 YAKLEEDYGLAKRAMKVYEEATKKVPEGQKLEMYEIYI 667
>At5g14060.1 68418.m01645 aspartate kinase, lysine-sensitive nearly
identical to gi:2257743
Length = 544
Score = 28.7 bits (61), Expect = 5.2
Identities = 16/34 (47%), Positives = 19/34 (55%)
Frame = +2
Query: 299 GGSILQGVLTVGMEIEVRPGLVSKDADGKLTCRP 400
GGS L T+G + +R V KD DG LTC P
Sbjct: 286 GGSDLTAT-TIGKALGLREIQVWKDVDGVLTCDP 318
>At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,
putative similar to mitochondrial elongation factor Tu
[Arabidopsis thaliana] gi|1149571|emb|CAA61511
Length = 454
Score = 28.7 bits (61), Expect = 5.2
Identities = 13/22 (59%), Positives = 16/22 (72%)
Frame = +2
Query: 284 RGGVAGGSILQGVLTVGMEIEV 349
RG VA G I QGV+ VG E+E+
Sbjct: 281 RGTVATGRIEQGVIKVGEEVEI 302
>At3g02020.1 68416.m00164 aspartate kinase, lysine-sensitive,
putative similar to aspartate kinase gi:2257743
(Arabidopsis thaliana)
Length = 559
Score = 28.7 bits (61), Expect = 5.2
Identities = 16/34 (47%), Positives = 19/34 (55%)
Frame = +2
Query: 299 GGSILQGVLTVGMEIEVRPGLVSKDADGKLTCRP 400
GGS L T+G + +R V KD DG LTC P
Sbjct: 287 GGSDLTAT-TIGKALGLREIQVWKDVDGVLTCDP 319
>At2g36360.1 68415.m04462 kelch repeat-containing protein low
similarity to rngB protein, Dictyostelium discoideum,
PIR:S68824; contains Pfam profile PF01344: Kelch motif
Length = 496
Score = 28.3 bits (60), Expect = 6.9
Identities = 10/23 (43%), Positives = 15/23 (65%)
Frame = +2
Query: 197 TPKGFHIASSYDCYSFIRCNKPG 265
TP+ FH+A + DC+ FI + G
Sbjct: 75 TPRAFHVAITIDCHMFIFGGRSG 97
>At1g64200.1 68414.m07273 vacuolar ATP synthase subunit E, putative
/ V-ATPase E subunit, putative / vacuolar proton pump E
subunit, putative similar to SP|Q39258 Vacuolar ATP
synthase subunit E (EC 3.6.3.14) (V-ATPase E subunit)
(Vacuolar proton pump E subunit) {Arabidopsis thaliana};
contains Pfam profile PF01991: ATP synthase (E/31 kDa)
subunit
Length = 237
Score = 28.3 bits (60), Expect = 6.9
Identities = 14/41 (34%), Positives = 25/41 (60%)
Frame = +3
Query: 45 VKEGQAKEQHEQIVKFVQGTVAEGAPIIPISAQLKYNIEVL 167
+ + A Q +Q+V+F++ E A I IS++ ++NIE L
Sbjct: 1 MNDADASIQIQQMVRFIRQEAEEKANEISISSEEEFNIEKL 41
>At5g46370.1 68418.m05707 outward rectifying potassium channel,
putative (KCO2) identical to KCO2 protein [Arabidopsis
thaliana] gi|6686780|emb|CAB64717; similar to kco1
[Arabidopsis thaliana] gi|2230761|emb|CAA69158; member
of the 2 pore, 4 transmembrane (2P/4TM) K+ channel
family, PMID:11500563
Length = 443
Score = 27.9 bits (59), Expect = 9.1
Identities = 14/38 (36%), Positives = 21/38 (55%), Gaps = 1/38 (2%)
Frame = -2
Query: 426 NSETMREN-MGRQVNLPSASLLTKPGRTSISIPTVRTP 316
N + +R+ M ++N P SLLT P ++IP TP
Sbjct: 10 NDDPLRQYLMNPRINPPPPSLLTLPENNDVTIPMPITP 47
>At4g16155.1 68417.m02451 dihydrolipoamide dehydrogenase 2,
plastidic / lipoamide dehydrogenase 2 (PTLPD2) identical
to plastidic lipoamide dehydrogenase from Arabidopsis
thaliana [gi:7159284]
Length = 567
Score = 27.9 bits (59), Expect = 9.1
Identities = 15/38 (39%), Positives = 23/38 (60%), Gaps = 3/38 (7%)
Frame = -1
Query: 109 ATVP*TNFTICSCCSLACPSLTKSILFCNIK---ICFS 5
A++P N T+CS S A PS +++ FC ++ CFS
Sbjct: 12 ASLPLANRTLCS--SNAAPSTPRNLRFCGLRREAFCFS 47
>At1g44740.1 68414.m05125 expressed protein ; expression supported
by MPSS
Length = 311
Score = 27.9 bits (59), Expect = 9.1
Identities = 12/35 (34%), Positives = 18/35 (51%)
Frame = +2
Query: 236 YSFIRCNKPGCEVDDLRGGVAGGSILQGVLTVGME 340
Y+ C +DD+R V S+ QGV+ +G E
Sbjct: 144 YNVSTCGGASLAIDDVRDAVRQVSVFQGVVPLGYE 178
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 19,686,424
Number of Sequences: 28952
Number of extensions: 438320
Number of successful extensions: 1046
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 996
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1044
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1980143200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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