Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0383
(738 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g23250.1 68418.m02720 succinyl-CoA ligase [GDP-forming] alpha... 100 9e-22
At5g08300.1 68418.m00977 succinyl-CoA ligase [GDP-forming] alpha... 100 9e-22
At2g23580.1 68415.m02814 hydrolase, alpha/beta fold family prote... 32 0.46
At2g23550.2 68415.m02811 hydrolase, alpha/beta fold family prote... 30 1.4
At2g23550.1 68415.m02810 hydrolase, alpha/beta fold family prote... 30 1.4
At2g23570.1 68415.m02813 hydrolase, alpha/beta fold family prote... 29 2.4
At5g57140.1 68418.m08530 calcineurin-like phosphoesterase family... 27 9.8
At4g21170.1 68417.m03062 pentatricopeptide (PPR) repeat-containi... 27 9.8
At2g44050.1 68415.m05476 6,7-dimethyl-8-ribityllumazine synthase... 27 9.8
At2g33640.1 68415.m04124 zinc finger (DHHC type) family protein ... 27 9.8
>At5g23250.1 68418.m02720 succinyl-CoA ligase [GDP-forming]
alpha-chain, mitochondrial, putative / succinyl-CoA
synthetase, alpha chain, putative / SCS-alpha, putative
similar to SP|P36967 Succinyl-CoA ligase [GDP-forming]
alpha-chain, mitochondrial precursor (EC 6.2.1.4)
(Succinyl-CoA synthetase, alpha chain) (SCS-alpha)
{Dictyostelium discoideum}; contains Pfam profiles
PF00549: CoA-ligase, PF02629: CoA binding domain
Length = 341
Score = 100 bits (240), Expect = 9e-22
Identities = 46/74 (62%), Positives = 52/74 (70%)
Frame = +3
Query: 510 PNCPGIIAPEKCKIGIMPAAVHKRGCIGVVSRSGTLTYEACHQTTITGLGKLCVSVLXGV 689
PNCPGII P +CKIGIMP +HK G IG+VSRSGTLTYEA QTT GLG+ + G
Sbjct: 167 PNCPGIIKPGECKIGIMPGYIHKPGKIGIVSRSGTLTYEAVFQTTAVGLGQSTCVGIGGD 226
Query: 690 PSTGQTFIDCLEVF 731
P G F+DCLE F
Sbjct: 227 PFNGTNFVDCLEKF 240
Score = 97.5 bits (232), Expect = 8e-21
Identities = 50/80 (62%), Positives = 54/80 (67%)
Frame = +1
Query: 256 GVSPKKAGTEHLGKPVFGTVKEAKAGTGATASVIYVPPPGXXXXXXXXXXXXMPLIVCIT 435
GV+PKK GTEHLG PVF TV EAKA T A ASVIYVP P + LIVCIT
Sbjct: 82 GVTPKKGGTEHLGLPVFNTVAEAKAETKANASVIYVPAPFAAAAIMEGLAAELDLIVCIT 141
Query: 436 EGVXQHDMVRVKHALLRQNK 495
EG+ QHDMVRVK AL Q+K
Sbjct: 142 EGIPQHDMVRVKAALNSQSK 161
Score = 48.8 bits (111), Expect = 4e-06
Identities = 19/34 (55%), Positives = 27/34 (79%)
Frame = +2
Query: 152 LILTSETKVIVQGFTGKQGTFHSQQALDYGTKVV 253
+ + T+VI QG TGK GTFH++QA++YGTK+V
Sbjct: 47 VFVDKNTRVICQGITGKNGTFHTEQAIEYGTKMV 80
>At5g08300.1 68418.m00977 succinyl-CoA ligase [GDP-forming]
alpha-chain, mitochondrial, putative / succinyl-CoA
synthetase, alpha chain, putative / SCS-alpha, putative
identical to SP|P53586 Succinyl-CoA ligase [GDP-forming]
alpha-chain, mitochondrial precursor (EC 6.2.1.4)
(Succinyl-CoA synthetase, alpha chain) (SCS-alpha)
{Arabidopsis thaliana}; strong similarity to SP|P13086
Succinyl-CoA ligase [GDP-forming] alpha-chain,
mitochondrial precursor {Rattus norvegicus}; contains
Pfam profiles PF00549: CoA-ligase, PF02629: CoA binding
domain
Length = 347
Score = 100 bits (240), Expect = 9e-22
Identities = 46/74 (62%), Positives = 52/74 (70%)
Frame = +3
Query: 510 PNCPGIIAPEKCKIGIMPAAVHKRGCIGVVSRSGTLTYEACHQTTITGLGKLCVSVLXGV 689
PNCPGII P +CKIGIMP +HK G IG+VSRSGTLTYEA QTT GLG+ + G
Sbjct: 172 PNCPGIIKPGECKIGIMPGYIHKPGKIGIVSRSGTLTYEAVFQTTAVGLGQSTCVGIGGD 231
Query: 690 PSTGQTFIDCLEVF 731
P G F+DCLE F
Sbjct: 232 PFNGTNFVDCLEKF 245
Score = 100 bits (239), Expect = 1e-21
Identities = 50/80 (62%), Positives = 55/80 (68%)
Frame = +1
Query: 256 GVSPKKAGTEHLGKPVFGTVKEAKAGTGATASVIYVPPPGXXXXXXXXXXXXMPLIVCIT 435
GV+PKK GTEHLG PVF +V EAKA T A ASVIYVP P + LIVCIT
Sbjct: 87 GVTPKKGGTEHLGLPVFNSVAEAKADTKANASVIYVPAPFAAAAIMEGIEAELDLIVCIT 146
Query: 436 EGVXQHDMVRVKHALLRQNK 495
EG+ QHDMVRVKHAL Q+K
Sbjct: 147 EGIPQHDMVRVKHALNSQSK 166
Score = 48.0 bits (109), Expect = 6e-06
Identities = 18/34 (52%), Positives = 27/34 (79%)
Frame = +2
Query: 152 LILTSETKVIVQGFTGKQGTFHSQQALDYGTKVV 253
+ + T+V+ QG TGK GTFH++QA++YGTK+V
Sbjct: 52 VFVDKNTRVLCQGITGKNGTFHTEQAIEYGTKMV 85
>At2g23580.1 68415.m02814 hydrolase, alpha/beta fold family protein
similar to ethylene-induced esterase [Citrus sinensis]
GI:14279437, polyneuridine aldehyde esterase [Rauvolfia
serpentina] GI:6651393; contains Pfam profile PF00561:
hydrolase, alpha/beta fold family
Length = 263
Score = 31.9 bits (69), Expect = 0.46
Identities = 15/33 (45%), Positives = 20/33 (60%)
Frame = +3
Query: 189 VSLESRVPSTANKPLTMVLKLLRSVTKEGWYRT 287
V L + +P T N P + KL+RSV +EGW T
Sbjct: 105 VFLTAFMPDTRNLPAYVYQKLIRSVPQEGWLDT 137
>At2g23550.2 68415.m02811 hydrolase, alpha/beta fold family protein
similar to ethylene-induced esterase [Citrus sinensis]
GI:14279437, polyneuridine aldehyde esterase [Rauvolfia
serpentina] GI:6651393; contains Pfam profile PF00561:
hydrolase, alpha/beta fold family
Length = 243
Score = 30.3 bits (65), Expect = 1.4
Identities = 17/43 (39%), Positives = 24/43 (55%)
Frame = +3
Query: 189 VSLESRVPSTANKPLTMVLKLLRSVTKEGWYRTSW*ACVWYSQ 317
V L + +P T N P + KLLRS+ +E W T+ CV Y +
Sbjct: 83 VFLTAFMPDTINPPAYVYEKLLRSIPQEEWLDTT---CVNYGK 122
>At2g23550.1 68415.m02810 hydrolase, alpha/beta fold family protein
similar to ethylene-induced esterase [Citrus sinensis]
GI:14279437, polyneuridine aldehyde esterase [Rauvolfia
serpentina] GI:6651393; contains Pfam profile PF00561:
hydrolase, alpha/beta fold family
Length = 272
Score = 30.3 bits (65), Expect = 1.4
Identities = 17/43 (39%), Positives = 24/43 (55%)
Frame = +3
Query: 189 VSLESRVPSTANKPLTMVLKLLRSVTKEGWYRTSW*ACVWYSQ 317
V L + +P T N P + KLLRS+ +E W T+ CV Y +
Sbjct: 112 VFLTAFMPDTINPPAYVYEKLLRSIPQEEWLDTT---CVNYGK 151
>At2g23570.1 68415.m02813 hydrolase, alpha/beta fold family protein
similar to ethylene-induced esterase [Citrus sinensis]
GI:14279437, polyneuridine aldehyde esterase [Rauvolfia
serpentina] GI:6651393
Length = 179
Score = 29.5 bits (63), Expect = 2.4
Identities = 13/35 (37%), Positives = 21/35 (60%)
Frame = +3
Query: 189 VSLESRVPSTANKPLTMVLKLLRSVTKEGWYRTSW 293
V L + +P T N P + KL+RS+ +E W T++
Sbjct: 21 VFLAAFMPDTRNPPAYVFEKLIRSIPREEWLDTAF 55
>At5g57140.1 68418.m08530 calcineurin-like phosphoesterase family
protein contains Pfam profile: PF00149 Calcineurin-like
phosphoesterase
Length = 397
Score = 27.5 bits (58), Expect = 9.8
Identities = 9/19 (47%), Positives = 13/19 (68%)
Frame = -3
Query: 67 PDWHSHCRIIECKSTKGKN 11
P+WH R+IE K KG++
Sbjct: 343 PNWHRRARVIEAKLGKGRD 361
>At4g21170.1 68417.m03062 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 534
Score = 27.5 bits (58), Expect = 9.8
Identities = 11/21 (52%), Positives = 13/21 (61%)
Frame = -3
Query: 79 LRKNPDWHSHCRIIECKSTKG 17
LR PD SHCR+IE + G
Sbjct: 97 LRFEPDLKSHCRVIEVAAESG 117
>At2g44050.1 68415.m05476 6,7-dimethyl-8-ribityllumazine synthase /
DMRL synthase / lumazine synthase / riboflavin synthase
identical to 6,7-dimethyl-8-ribityllumazine synthase,
chloroplast [precursor] SP:O80575 from [Arabidopsis
thaliana]
Length = 227
Score = 27.5 bits (58), Expect = 9.8
Identities = 20/62 (32%), Positives = 29/62 (46%), Gaps = 6/62 (9%)
Frame = +3
Query: 525 IIAPEKCKIGIMPAAVHKRG------CIGVVSRSGTLTYEACHQTTITGLGKLCVSVLXG 686
I P +IG++ + K G CIG V R T Y+A + +G+ L S+ G
Sbjct: 123 IWVPGSFEIGVVAQNLGKSGKFHAVLCIGAVIRGDTTHYDAVANSAASGV--LSASINSG 180
Query: 687 VP 692
VP
Sbjct: 181 VP 182
>At2g33640.1 68415.m04124 zinc finger (DHHC type) family protein
contains Pfam profile PF01529: DHHC zinc finger domain
Length = 565
Score = 27.5 bits (58), Expect = 9.8
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = -1
Query: 390 NGCCSSRGRYINNRGSCSCACLCLFDCTK 304
+GCCS+ GR+I C CL + DC +
Sbjct: 109 SGCCSAIGRFI-------CGCLVIQDCRR 130
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,480,538
Number of Sequences: 28952
Number of extensions: 318465
Number of successful extensions: 757
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 733
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 754
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1624036432
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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