BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e96h0380 (698 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g32670.1 68417.m04651 expressed protein ; expression supporte... 31 0.97 At3g28010.1 68416.m03497 hypothetical protein contains Pfam prof... 28 6.8 At3g44880.1 68416.m04835 Rieske [2Fe-2S] domain-containing prote... 27 9.0 At1g71840.1 68414.m08302 transducin family protein / WD-40 repea... 27 9.0 At1g59580.2 68414.m06701 mitogen-activated protein kinase, putat... 27 9.0 At1g59580.1 68414.m06700 mitogen-activated protein kinase, putat... 27 9.0 >At4g32670.1 68417.m04651 expressed protein ; expression supported by MPSS Length = 808 Score = 30.7 bits (66), Expect = 0.97 Identities = 13/65 (20%), Positives = 33/65 (50%) Frame = -3 Query: 348 YLITFDNKN*IILFINLVHLDSRLCICFFLISVLIFESLLYHVGFLVGRFVFLYLLPILQ 169 ++++F K+ + F+NL LCI ++ +I + + +GF + R +++ + Sbjct: 548 FMLSFGLKHDGLYFLNLASHVFLLCIMLLILKPIILDLCAFWIGFCILRGIYIITCFVYD 607 Query: 168 NILVG 154 + + G Sbjct: 608 HFITG 612 >At3g28010.1 68416.m03497 hypothetical protein contains Pfam profile PF03778: Protein of unknown function (DUF321) Length = 261 Score = 27.9 bits (59), Expect = 6.8 Identities = 12/32 (37%), Positives = 17/32 (53%) Frame = -1 Query: 287 IPDFAFVSSSYLY*FLNRFSIMLVFWWEDLSF 192 I F F +Y + FL S + VFWW++ F Sbjct: 220 ITGFGFWRENYEFTFLAEKSRVYVFWWKNYEF 251 >At3g44880.1 68416.m04835 Rieske [2Fe-2S] domain-containing protein similar to lethal leaf-spot 1 from Zea mays [gi:1935909]; contains Pfam PF00355 Rieske [2Fe-2S] domain Length = 537 Score = 27.5 bits (58), Expect = 9.0 Identities = 9/16 (56%), Positives = 13/16 (81%) Frame = +1 Query: 73 KLWVIGN*KWVGWLCT 120 KL ++GN KWV W+C+ Sbjct: 311 KLPIVGNQKWVIWICS 326 >At1g71840.1 68414.m08302 transducin family protein / WD-40 repeat family protein contains Pfam profile:PF00560 Leucine Rich Repeat (4 copies); Pfam profile:PF00069 Eukaryotic protein kinase domain; Pfam profile:PF00400 WD domain, G-beta repeat (7 copies) Length = 407 Score = 27.5 bits (58), Expect = 9.0 Identities = 13/35 (37%), Positives = 17/35 (48%) Frame = +1 Query: 76 LWVIGN*KWVGWLCTHHFLLDCSFFSTNQNILKNG 180 LW IGN W L H + C FS + +L +G Sbjct: 97 LWKIGNGDWAAELPGHKDSVSCLAFSYDGQLLASG 131 >At1g59580.2 68414.m06701 mitogen-activated protein kinase, putative / MAPK, putative (MPK2) identical to mitogen-activated protein kinase homolog 2 (AtMPK2)[Arabidopsis thaliana] SWISS-PROT:Q39022; PMID:12119167 Length = 376 Score = 27.5 bits (58), Expect = 9.0 Identities = 11/21 (52%), Positives = 15/21 (71%) Frame = -2 Query: 400 SIH*NSNEIIVIIKVHNVFNN 338 S++ SNE + I K+HNVF N Sbjct: 49 SVNRESNERVAIKKIHNVFEN 69 >At1g59580.1 68414.m06700 mitogen-activated protein kinase, putative / MAPK, putative (MPK2) identical to mitogen-activated protein kinase homolog 2 (AtMPK2)[Arabidopsis thaliana] SWISS-PROT:Q39022; PMID:12119167 Length = 376 Score = 27.5 bits (58), Expect = 9.0 Identities = 11/21 (52%), Positives = 15/21 (71%) Frame = -2 Query: 400 SIH*NSNEIIVIIKVHNVFNN 338 S++ SNE + I K+HNVF N Sbjct: 49 SVNRESNERVAIKKIHNVFEN 69 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,874,400 Number of Sequences: 28952 Number of extensions: 277249 Number of successful extensions: 551 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 542 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 550 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1496852856 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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