BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0370
(665 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC664.05 |rpl13||60S ribosomal protein L13|Schizosaccharomyces... 89 5e-19
SPBC1289.15 ||SPBC8E4.07c|glycoprotein |Schizosaccharomyces pomb... 29 0.80
SPAC6F12.10c |ade3|min11|phosphoribosylformylglycinamidine synth... 27 2.4
SPAC25G10.07c |cut7||kinesin-like protein Cut7|Schizosaccharomyc... 27 3.2
SPCC622.10c |||exocyst complex subunit Sec5 |Schizosaccharomyces... 27 3.2
SPCC1322.14c |vtc4||vacuolar transporter chaperone |Schizosaccha... 25 9.8
>SPAC664.05 |rpl13||60S ribosomal protein L13|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 208
Score = 89.0 bits (211), Expect = 5e-19
Identities = 40/74 (54%), Positives = 49/74 (66%)
Frame = +2
Query: 35 IPNGHFHKDWQRFVKTWFNQPARRYRRKQNRIKKXXXXXXXXXXXXXXXIVRCPTVRYHT 214
+PN HFHKDWQR+VKTWFNQP R+ RR+Q R K V+ PT+RY+
Sbjct: 9 LPNAHFHKDWQRYVKTWFNQPGRKLRRRQARQTK-AAKIAPRPVEAIRPAVKPPTIRYNM 67
Query: 215 KVRAGRGFTLREIR 256
KVRAGRGFTL E++
Sbjct: 68 KVRAGRGFTLEELK 81
Score = 57.2 bits (132), Expect = 2e-09
Identities = 36/78 (46%), Positives = 48/78 (61%), Gaps = 1/78 (1%)
Frame = +3
Query: 279 ARTIGSAVDPRRRNKSVESLQINVQRIKEYRARLILFP-KGKKVLKGEANEEERKLATQL 455
A TIG VD RRRN+S ESLQ NV+RIK Y A LI+FP K + KG+A + T +
Sbjct: 90 ASTIGIPVDHRRRNRSEESLQRNVERIKVYLAHLIVFPRKAGQPKKGDATDVSGAEQTDV 149
Query: 456 RGPLMPVQQPAPKSVARP 509
++P+ Q A + A+P
Sbjct: 150 -AAVLPITQEAVEE-AKP 165
Score = 33.9 bits (74), Expect = 0.021
Identities = 17/41 (41%), Positives = 22/41 (53%)
Frame = +2
Query: 503 KTITEDEKNFKAYQYLRGARSIAKLVGIRAKRLKDAAENPD 625
K ITE+ KNF A+ L R+ A+ G RA K AE +
Sbjct: 164 KPITEEAKNFNAFSTLSNERAYARYAGARAAFQKKRAEEAE 204
>SPBC1289.15 ||SPBC8E4.07c|glycoprotein |Schizosaccharomyces pombe|chr
2|||Manual
Length = 1283
Score = 28.7 bits (61), Expect = 0.80
Identities = 16/44 (36%), Positives = 20/44 (45%), Gaps = 4/44 (9%)
Frame = -2
Query: 247 TKSESSTGAYFSM----VPNSWASHYRT*RPSCRTWSYGXSFLY 128
T +STG+Y M + W S T C TWSY S+ Y
Sbjct: 1215 TVQGTSTGSYICMPHFQIQYDWCSAGVTDMSECNTWSYQKSYDY 1258
>SPAC6F12.10c |ade3|min11|phosphoribosylformylglycinamidine synthase
Ade3 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1323
Score = 27.1 bits (57), Expect = 2.4
Identities = 14/27 (51%), Positives = 16/27 (59%)
Frame = +2
Query: 227 GRGFTLREIRPQIEPSICPNDWKCCRS 307
G F LR+I P IEPS+ P CC S
Sbjct: 527 GARFELRDI-PCIEPSMSPMQIWCCES 552
>SPAC25G10.07c |cut7||kinesin-like protein Cut7|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1085
Score = 26.6 bits (56), Expect = 3.2
Identities = 11/31 (35%), Positives = 20/31 (64%)
Frame = +2
Query: 527 NFKAYQYLRGARSIAKLVGIRAKRLKDAAEN 619
N ++ ++LR A S A++VG +R++ EN
Sbjct: 843 NNRSEEFLRNAASQAEIVGANKERIQKTVEN 873
>SPCC622.10c |||exocyst complex subunit Sec5 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 815
Score = 26.6 bits (56), Expect = 3.2
Identities = 12/39 (30%), Positives = 20/39 (51%)
Frame = -3
Query: 303 LQHFQSFGQILGSICGLISRRVNPLPARTLVWYRTVGHR 187
LQ F ++ ++C L+ + NP+ R V Y TV +
Sbjct: 292 LQSQGDFAFVVRTLCDLLKLKPNPISGRDPVLYATVSQQ 330
>SPCC1322.14c |vtc4||vacuolar transporter chaperone
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 721
Score = 25.0 bits (52), Expect = 9.8
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = +3
Query: 39 LMDISTRIGKDLLKLGLTSQLDDTAES 119
L+DIS R G+D L S+L D +S
Sbjct: 556 LLDISKRKGRDSFVAALNSRLKDIKDS 582
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,665,453
Number of Sequences: 5004
Number of extensions: 52966
Number of successful extensions: 155
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 148
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 154
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 303841898
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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