Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= e96h0354
         (469 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC26F1.06 |gpm1||monomeric 2,3-bisphosphoglycerate |Schizosacc...    84   1e-17
SPAC732.02c |||fructose-2,6-bisphosphate 2-phosphatase activity ...    31   0.066
SPCC1620.13 |||phosphoglycerate mutase family|Schizosaccharomyce...    30   0.20 
SPAC1687.21 ||SPAC222.01|phosphoglycerate mutase family |Schizos...    29   0.27 
SPCP25A2.03 |||THO complex subunit |Schizosaccharomyces pombe|ch...    28   0.82 
SPCC645.08c |snd1||RNA-binding protein Snd1|Schizosaccharomyces ...    27   1.4  
SPCC1020.13c ||SPCC14G10.05|phospholipase |Schizosaccharomyces p...    26   3.3  
SPAC31G5.12c |maf1|n150|repressor of RNA polymerase III Maf1 |Sc...    25   4.4  
SPCC736.14 |dis1||microtubule-associated protein Dis1 |Schizosac...    25   4.4  
SPCP1E11.11 |||Puf family RNA-binding protein|Schizosaccharomyce...    25   7.6  

>SPAC26F1.06 |gpm1||monomeric 2,3-bisphosphoglycerate
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 211

 Score = 83.8 bits (198), Expect = 1e-17
 Identities = 37/68 (54%), Positives = 50/68 (73%)
 Frame = +1

Query: 259 TSVLKRAQITLNSILKEIGQPDIPVEKTWRLNERHYGGLTGLNKAETAAKYGEAQVQIWR 438
           TS L+RAQ T   IL+E+G+P++   K+ +LNER+YG L GLNK +   K+G  QVQIWR
Sbjct: 61  TSALQRAQKTCQIILEEVGEPNLETIKSEKLNERYYGDLQGLNKDDARKKWGAEQVQIWR 120

Query: 439 RSFDVPPP 462
           RS+D+ PP
Sbjct: 121 RSYDIAPP 128



 Score = 65.7 bits (153), Expect = 3e-12
 Identities = 28/49 (57%), Positives = 36/49 (73%)
 Frame = +2

Query: 107 IVMIRHGESEWNQKNLFCGWFDADLSDKGRQEAVAAGKALKAEGYQFDI 253
           +V+ RHGESEWN+ NLF GW D  LS+ G +EA   G+ LK+ GY+FDI
Sbjct: 10  LVLTRHGESEWNKLNLFTGWKDPALSETGIKEAKLGGERLKSRGYKFDI 58


>SPAC732.02c |||fructose-2,6-bisphosphate 2-phosphatase activity
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 408

 Score = 31.5 bits (68), Expect = 0.066
 Identities = 19/49 (38%), Positives = 27/49 (55%)
 Frame = +2

Query: 59  SRYEIYLSNKMPAKYKIVMIRHGESEWNQKNLFCGWFDADLSDKGRQEA 205
           SR   YLSN    +  I + RHGES++N +    G  D+ LS +G + A
Sbjct: 193 SRIVYYLSNLRTRRRSIWLSRHGESQFNVEGKIGG--DSSLSPQGLKYA 239


>SPCC1620.13 |||phosphoglycerate mutase family|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 282

 Score = 29.9 bits (64), Expect = 0.20
 Identities = 14/51 (27%), Positives = 30/51 (58%), Gaps = 1/51 (1%)
 Frame = +2

Query: 80  SNKMPAKYKIVMIRHGESEWNQKNLFCG-WFDADLSDKGRQEAVAAGKALK 229
           S++  +K+  +++RH ESE N + +  G   D++L+  G  +A    K+++
Sbjct: 47  SSQNDSKFTCLLVRHAESEHNVRGIRAGARIDSELTVHGYNQAKKLAKSIR 97


>SPAC1687.21 ||SPAC222.01|phosphoglycerate mutase family
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 209

 Score = 29.5 bits (63), Expect = 0.27
 Identities = 16/45 (35%), Positives = 26/45 (57%)
 Frame = +1

Query: 262 SVLKRAQITLNSILKEIGQPDIPVEKTWRLNERHYGGLTGLNKAE 396
           S +KR + T+   L+   +P++P+  T  + ER YG L G+N  E
Sbjct: 53  SSMKRCRETIAPYLEL--KPEVPIVYTDLIRERVYGDLEGMNVVE 95


>SPCP25A2.03 |||THO complex subunit |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 752

 Score = 27.9 bits (59), Expect = 0.82
 Identities = 16/52 (30%), Positives = 26/52 (50%)
 Frame = +2

Query: 47  SVICSRYEIYLSNKMPAKYKIVMIRHGESEWNQKNLFCGWFDADLSDKGRQE 202
           S +CS YE+++    P+   IV   H  +E+     FC +  A  S+ G +E
Sbjct: 26  SPLCSEYELFIKELWPS---IVESFHNSTEFETAIRFCCYETARKSEIGLEE 74


>SPCC645.08c |snd1||RNA-binding protein Snd1|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 878

 Score = 27.1 bits (57), Expect = 1.4
 Identities = 12/32 (37%), Positives = 21/32 (65%), Gaps = 1/32 (3%)
 Frame = -2

Query: 465 GRWRNVEAAAPDLN-LSLPVFGSCLSLVQSSE 373
           G W+N+  + PD+N LSL  + + +S V S++
Sbjct: 308 GIWKNISVSIPDINSLSLKDYSAVVSRVISTD 339


>SPCC1020.13c ||SPCC14G10.05|phospholipase |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 669

 Score = 25.8 bits (54), Expect = 3.3
 Identities = 12/34 (35%), Positives = 18/34 (52%)
 Frame = +2

Query: 62  RYEIYLSNKMPAKYKIVMIRHGESEWNQKNLFCG 163
           R+  Y +N + AKY  ++      E NQKN+  G
Sbjct: 40  RFLNYDNNALEAKYNEIITEEVSQEPNQKNVIVG 73


>SPAC31G5.12c |maf1|n150|repressor of RNA polymerase III Maf1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 215

 Score = 25.4 bits (53), Expect = 4.4
 Identities = 11/32 (34%), Positives = 17/32 (53%)
 Frame = +1

Query: 268 LKRAQITLNSILKEIGQPDIPVEKTWRLNERH 363
           L R   ++NS L  IG+  + V   W + +RH
Sbjct: 95  LSRVVDSVNSTLNNIGRGRLSVNGIWEIIDRH 126


>SPCC736.14 |dis1||microtubule-associated protein Dis1
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 882

 Score = 25.4 bits (53), Expect = 4.4
 Identities = 9/16 (56%), Positives = 12/16 (75%)
 Frame = +1

Query: 112 YDSSWRIRMEPEESLL 159
           +D SW++R E  ESLL
Sbjct: 15  FDKSWKVRFEAYESLL 30


>SPCP1E11.11 |||Puf family RNA-binding protein|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 642

 Score = 24.6 bits (51), Expect = 7.6
 Identities = 19/88 (21%), Positives = 41/88 (46%), Gaps = 3/88 (3%)
 Frame = +2

Query: 8   TSKRKYRGFSSVLSVICSRYEIYLSNKMPAKYKIVMIRHGESEWNQKNLF---CGWFDAD 178
           T+K  Y     V ++ C+   I     + A+++  +++    ++ ++ L     GW DA 
Sbjct: 390 TAKDSYGHLVVVAALDCTDDTIMTGKLLQAEFEGELLKLSADKFARRILLYVLVGWEDAR 449

Query: 179 LSDKGRQEAVAAGKALKAEGYQFDIVTR 262
              K  +E + +  +LKA+  + D + R
Sbjct: 450 YFSKENRELLRSLDSLKAKTSKKDPIVR 477


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,898,966
Number of Sequences: 5004
Number of extensions: 35172
Number of successful extensions: 92
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 91
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 92
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 178394480
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)