Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0354
(469 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g22170.1 68414.m02771 phosphoglycerate/bisphosphoglycerate mu... 72 2e-13
At1g78050.1 68414.m09095 phosphoglycerate/bisphosphoglycerate mu... 70 7e-13
At3g50520.1 68416.m05525 phosphoglycerate/bisphosphoglycerate mu... 44 5e-05
At5g04120.1 68418.m00398 phosphoglycerate/bisphosphoglycerate mu... 40 6e-04
At1g65540.1 68414.m07435 calcium-binding EF hand family protein ... 33 0.13
At5g22620.1 68418.m02643 phosphoglycerate/bisphosphoglycerate mu... 31 0.29
At4g17510.1 68417.m02620 ubiquitin carboxyl-terminal hydrolase, ... 31 0.51
At4g12870.1 68417.m02015 gamma interferon responsive lysosomal t... 31 0.51
At3g59820.1 68416.m06675 calcium-binding mitochondrial protein-r... 28 3.6
At4g38260.1 68417.m05404 expressed protein contains Pfam PF05742... 27 4.8
At1g08940.1 68414.m00995 phosphoglycerate/bisphosphoglycerate mu... 27 4.8
At4g20860.1 68417.m03026 FAD-binding domain-containing protein s... 27 6.3
At3g11920.1 68416.m01461 glutaredoxin-related contains INTERPRO ... 27 8.4
At3g05170.1 68416.m00564 phosphoglycerate/bisphosphoglycerate mu... 27 8.4
>At1g22170.1 68414.m02771 phosphoglycerate/bisphosphoglycerate
mutase family protein similar to SP|P31217
Phosphoglycerate mutase 1 (EC 5.4.2.1) {Escherichia coli
O157:H7}; contains Pfam profile PF00300:
phosphoglycerate mutase family
Length = 334
Score = 71.7 bits (168), Expect = 2e-13
Identities = 31/52 (59%), Positives = 36/52 (69%)
Frame = +1
Query: 307 EIGQPDIPVEKTWRLNERHYGGLTGLNKAETAAKYGEAQVQIWRRSFDVPPP 462
E IPV W+LNER YG L GLNK ETA +YG+ QV WRRS+D+PPP
Sbjct: 172 ETKNQSIPVIPAWQLNERMYGELQGLNKQETAERYGKEQVHEWRRSYDIPPP 223
Score = 56.4 bits (130), Expect = 9e-09
Identities = 24/49 (48%), Positives = 34/49 (69%)
Frame = +2
Query: 80 SNKMPAKYKIVMIRHGESEWNQKNLFCGWFDADLSDKGRQEAVAAGKAL 226
S K + +++IRHGES WN+KNLF G D L++KG +EA+ AGK +
Sbjct: 71 SQKKSNEAALILIRHGESLWNEKNLFTGCVDVPLTEKGVEEAIEAGKRI 119
>At1g78050.1 68414.m09095 phosphoglycerate/bisphosphoglycerate
mutase family protein similar to SP|P31217
Phosphoglycerate mutase 1 (EC 5.4.2.1) {Escherichia coli
O157:H7}; contains Pfam profile PF00300:
phosphoglycerate mutase family
Length = 332
Score = 70.1 bits (164), Expect = 7e-13
Identities = 30/53 (56%), Positives = 37/53 (69%)
Frame = +1
Query: 304 KEIGQPDIPVEKTWRLNERHYGGLTGLNKAETAAKYGEAQVQIWRRSFDVPPP 462
+E + IPV W+LNER YG L GLNK ETA +YG QV WRRS+++PPP
Sbjct: 172 EETRKQSIPVIAAWQLNERMYGELQGLNKKETAERYGTQQVHEWRRSYEIPPP 224
Score = 55.2 bits (127), Expect = 2e-08
Identities = 27/64 (42%), Positives = 40/64 (62%)
Frame = +2
Query: 35 SSVLSVICSRYEIYLSNKMPAKYKIVMIRHGESEWNQKNLFCGWFDADLSDKGRQEAVAA 214
++ LS S+ + + S K + +++IRHGES WN+KNLF G D L+ KG EA+ A
Sbjct: 57 NTFLSPSPSKNKPHESKKKSNEAALILIRHGESLWNEKNLFTGCVDVPLTQKGVGEAIEA 116
Query: 215 GKAL 226
GK +
Sbjct: 117 GKKI 120
>At3g50520.1 68416.m05525 phosphoglycerate/bisphosphoglycerate
mutase family protein similar to SP|P00950
Phosphoglycerate mutase 1 (EC 5.4.2.1) {Saccharomyces
cerevisiae}; contains Pfam profile PF00300:
phosphoglycerate mutase family
Length = 230
Score = 44.0 bits (99), Expect = 5e-05
Identities = 18/44 (40%), Positives = 27/44 (61%)
Frame = +2
Query: 104 KIVMIRHGESEWNQKNLFCGWFDADLSDKGRQEAVAAGKALKAE 235
+IV++RHGE+ WN + G D +L+D GRQ+A + L E
Sbjct: 15 EIVVVRHGETSWNAERKIQGHLDVELNDAGRQQAQRVAERLSKE 58
>At5g04120.1 68418.m00398 phosphoglycerate/bisphosphoglycerate
mutase family protein low similarity to SP|P36623
Phosphoglycerate mutase (EC 5.4.2.1)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00300: phosphoglycerate mutase family
Length = 238
Score = 40.3 bits (90), Expect = 6e-04
Identities = 17/44 (38%), Positives = 28/44 (63%)
Frame = +2
Query: 104 KIVMIRHGESEWNQKNLFCGWFDADLSDKGRQEAVAAGKALKAE 235
+IV++RHGE+ WN G ++DL++ G ++AVA + L E
Sbjct: 26 EIVLVRHGETTWNAAGRIQGQIESDLNEVGLKQAVAIAERLGKE 69
>At1g65540.1 68414.m07435 calcium-binding EF hand family protein
similar to leucine zipper-EF-hand containing
transmembrane protein 1 [Homo sapiens] GI:4235226;
contains Pfam profile PF00036: EF hand
Length = 736
Score = 32.7 bits (71), Expect = 0.13
Identities = 16/51 (31%), Positives = 25/51 (49%)
Frame = +1
Query: 259 TSVLKRAQITLNSILKEIGQPDIPVEKTWRLNERHYGGLTGLNKAETAAKY 411
+++L R + L + KEI D + WRL +R Y G ++ AA Y
Sbjct: 634 SALLDRVETMLQKLEKEIDDVDNKIGNRWRLLDRDYDGKVSPDEVALAAMY 684
>At5g22620.1 68418.m02643 phosphoglycerate/bisphosphoglycerate
mutase family protein weak similarity to SP|P15259
Phosphoglycerate mutase, muscle form (EC 5.4.2.1 {Homo
sapiens}; contains Pfam profile PF00300:
phosphoglycerate mutase family
Length = 482
Score = 31.5 bits (68), Expect = 0.29
Identities = 13/35 (37%), Positives = 23/35 (65%), Gaps = 1/35 (2%)
Frame = +2
Query: 104 KIVMIRHGESEWNQKNLFCGWFD-ADLSDKGRQEA 205
++V++RHG+S WN++ G D + L+ KG +A
Sbjct: 49 RVVLVRHGQSTWNEEGRIQGSSDFSVLTKKGESQA 83
>At4g17510.1 68417.m02620 ubiquitin carboxyl-terminal hydrolase,
putative / ubiquitin thiolesterase, putative similar to
SP|Q9JKB1 Ubiquitin carboxyl-terminal hydrolase isozyme
L3 (EC 3.4.19.12) (UCH- L3) (Ubiquitin thiolesterase L3)
{Mus musculus}; contains Pfam profile PF01088: Ubiquitin
carboxyl-terminal hydrolase, family 1
Length = 234
Score = 30.7 bits (66), Expect = 0.51
Identities = 12/33 (36%), Positives = 18/33 (54%)
Frame = -2
Query: 381 SSEATIMPLVQSPSFLNRYIWLTDLL*DRVQCN 283
SS +PL +P +N+Y+W L D +CN
Sbjct: 8 SSSKRWLPLESNPDVMNQYLWGLGLAPDEAECN 40
>At4g12870.1 68417.m02015 gamma interferon responsive lysosomal
thiol reductase family protein / GILT family protein
similar to SP|P13284 Gamma-interferon inducible
lysosomal thiol reductase precursor {Homo sapiens};
contains Pfam profile PF03227: Gamma interferon
inducible lysosomal thiol reductase (GILT)
Length = 229
Score = 30.7 bits (66), Expect = 0.51
Identities = 17/58 (29%), Positives = 31/58 (53%), Gaps = 2/58 (3%)
Frame = -1
Query: 256 DNVKLIAFSF*SLTGSNSFLTTFVAEV--SVEPSTEEILLVPFGFAMTNHNNLILCRH 89
D V+L + G SF+ + +V S + ++ LVPFG+A ++N ++C+H
Sbjct: 31 DKVELNLYYESLCPGCQSFIVDELVKVFDSDLDTITDVKLVPFGYAKVSNNLTVICQH 88
>At3g59820.1 68416.m06675 calcium-binding mitochondrial
protein-related contains weak similarity to
Calcium-binding mitochondrial protein Anon-60Da
(Swiss-Prot:P91927) [Drosophila melanogaster]
Length = 755
Score = 27.9 bits (59), Expect = 3.6
Identities = 13/51 (25%), Positives = 27/51 (52%)
Frame = +1
Query: 259 TSVLKRAQITLNSILKEIGQPDIPVEKTWRLNERHYGGLTGLNKAETAAKY 411
++++++ + ++ KEI DI + K W+L +R G ++ AA Y
Sbjct: 655 SALMEKVDGLIQNLEKEIDDVDIKIGKGWQLLDRDRDGKVTPDEVAAAAMY 705
>At4g38260.1 68417.m05404 expressed protein contains Pfam PF05742:
Protein of unknown function (DUF833)
Length = 275
Score = 27.5 bits (58), Expect = 4.8
Identities = 15/49 (30%), Positives = 26/49 (53%), Gaps = 1/49 (2%)
Frame = +2
Query: 23 YRGFSSVLSVICSRYEIYLSNKMPAKYKIV-MIRHGESEWNQKNLFCGW 166
Y GF+ V++ + S+ IY++N+ P K+V + G + NL W
Sbjct: 108 YNGFNLVVAHVLSKSMIYITNRPPHGDKLVTQVSPGIHVLSNANLDSPW 156
>At1g08940.1 68414.m00995 phosphoglycerate/bisphosphoglycerate
mutase family protein contains Pfam profile PF00300:
phosphoglycerate mutase family
Length = 281
Score = 27.5 bits (58), Expect = 4.8
Identities = 16/46 (34%), Positives = 27/46 (58%), Gaps = 3/46 (6%)
Frame = +2
Query: 104 KIVMIRHGESEWN-QKNLFCGWFD--ADLSDKGRQEAVAAGKALKA 232
+I+++RHGES N + D L+++GR +A AGK ++A
Sbjct: 11 RIILMRHGESAGNIDAGAYATTPDHKIPLTEEGRAQAREAGKKMRA 56
>At4g20860.1 68417.m03026 FAD-binding domain-containing protein
simlar to SP|P30986 reticuline oxidase precursor
(Berberine-bridge-forming enzyme) (BBE)
(Tetrahydroprotoberberine synthase) [Eschscholzia
californica]; contains PF01565 FAD binding domain
Length = 530
Score = 27.1 bits (57), Expect = 6.3
Identities = 12/39 (30%), Positives = 17/39 (43%)
Frame = +1
Query: 10 VKEKIPRFFIGAISNL*PVRNLSIKQNACKV*DCYDSSW 126
VK F+G I L P+ N + + DC + SW
Sbjct: 303 VKSTFQTLFLGGIDRLIPLMNQKFPELGLRSQDCSEMSW 341
>At3g11920.1 68416.m01461 glutaredoxin-related contains INTERPRO
Domain IPR002109, Glutaredoxin (thioltransferase)
Length = 630
Score = 26.6 bits (56), Expect = 8.4
Identities = 12/41 (29%), Positives = 19/41 (46%), Gaps = 2/41 (4%)
Frame = +1
Query: 352 NERHYGGLTGLNKAETAAKYGEAQVQIWRRSF--DVPPPAM 468
NE+H+GGL LN + ++ + + D P P M
Sbjct: 214 NEKHFGGLMALNSLRNSGEFDRRVKEFLKEKCCGDAPLPVM 254
>At3g05170.1 68416.m00564 phosphoglycerate/bisphosphoglycerate
mutase family protein contains Pfam profile PF00300:
phosphoglycerate mutase family
Length = 316
Score = 26.6 bits (56), Expect = 8.4
Identities = 20/53 (37%), Positives = 28/53 (52%), Gaps = 3/53 (5%)
Frame = +2
Query: 83 NKMPAKYKIVMIRHGESEWN-QKNLFCGWFD--ADLSDKGRQEAVAAGKALKA 232
NK+ K +I+++RHGESE N + D L+D G +A AG L A
Sbjct: 5 NKLLPK-RIILVRHGESEGNLDTAAYTTTPDHKIQLTDSGLLQAQEAGARLHA 56
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,038,342
Number of Sequences: 28952
Number of extensions: 186477
Number of successful extensions: 490
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 482
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 489
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 791932800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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