Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0331
(566 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 178 5e-46
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 92 5e-20
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 87 2e-18
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 73 4e-14
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 48 1e-06
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 44 2e-05
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 41 1e-04
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 27 1.9
SPAC13F5.04c |||endosomal sorting protein |Schizosaccharomyces p... 27 1.9
SPAC22F8.08 |||COPII vesicle coat protein |Schizosaccharomyces p... 26 4.4
SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual 26 4.4
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 26 4.4
SPCP1E11.04c |pal1||membrane associated protein Pal1 |Schizosacc... 25 7.7
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 178 bits (433), Expect = 5e-46
Identities = 78/86 (90%), Positives = 81/86 (94%)
Frame = +3
Query: 255 GRPRHQGVMVGMGQKDSXVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELR 434
GRPRH G+MVGMGQKDS VGDEAQSKRGILTLKYPIEHGIV NWDDMEKIWHHTFYNELR
Sbjct: 36 GRPRHHGIMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELR 95
Query: 435 VAPEEHPVLLTEAPLNPKANREKMNR 512
VAPEEHP LLTEAPLNPK+NREKM +
Sbjct: 96 VAPEEHPCLLTEAPLNPKSNREKMTQ 121
Score = 72.9 bits (171), Expect = 3e-14
Identities = 32/37 (86%), Positives = 35/37 (94%)
Frame = +1
Query: 154 DEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVEGP 264
+EE+AALV+DNGSGMCKAGFAGDDAPRAVFPSIV P
Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRP 38
Score = 36.3 bits (80), Expect = 0.003
Identities = 16/28 (57%), Positives = 20/28 (71%)
Frame = +2
Query: 482 PQGQQREDEQIMFETXNTPAMYVAIQAV 565
P+ + + QI+FET N PA YVAIQAV
Sbjct: 112 PKSNREKMTQIIFETFNAPAFYVAIQAV 139
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 92.3 bits (219), Expect = 5e-20
Identities = 43/85 (50%), Positives = 58/85 (68%), Gaps = 1/85 (1%)
Frame = +3
Query: 255 GRPRHQGVMVGMGQKDSXVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYN-EL 431
GR +H+ VM QKD VG EAQ+ RG+L ++ PIE GI+ NW DME+IW + + + +L
Sbjct: 40 GRIKHERVMPSSIQKDMFVGSEAQNLRGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQL 99
Query: 432 RVAPEEHPVLLTEAPLNPKANREKM 506
PEEHP+LLTE PL N+EK+
Sbjct: 100 NTLPEEHPLLLTEPPLANIRNKEKI 124
Score = 44.4 bits (100), Expect = 1e-05
Identities = 17/28 (60%), Positives = 22/28 (78%)
Frame = +1
Query: 172 LVVDNGSGMCKAGFAGDDAPRAVFPSIV 255
+ +DNGSG KAGFAGDD P+ +FP+ V
Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCV 39
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 86.6 bits (205), Expect = 2e-18
Identities = 34/70 (48%), Positives = 54/70 (77%)
Frame = +3
Query: 297 KDSXVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAP 476
KD VGDEA++ R +L +KYP+E+GI+ ++++M ++W +TF+ +L++ P +LLTE P
Sbjct: 50 KDVMVGDEAEAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPP 109
Query: 477 LNPKANREKM 506
+NP ANREKM
Sbjct: 110 MNPVANREKM 119
Score = 46.0 bits (104), Expect = 4e-06
Identities = 21/39 (53%), Positives = 27/39 (69%)
Frame = +1
Query: 160 EVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVEGPAIRA 276
E A +V+DNG+G K G+A D+ PR FPSIV P +RA
Sbjct: 2 ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRA 40
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 72.5 bits (170), Expect = 4e-14
Identities = 32/68 (47%), Positives = 41/68 (60%), Gaps = 1/68 (1%)
Frame = +3
Query: 300 DSXVGDEAQSKRGI-LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAP 476
D +G++A K +L YPI HG + NWD ME+ W + + LR PE+H LLTE P
Sbjct: 73 DFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPP 132
Query: 477 LNPKANRE 500
LNP NRE
Sbjct: 133 LNPPENRE 140
Score = 40.7 bits (91), Expect = 1e-04
Identities = 14/28 (50%), Positives = 23/28 (82%)
Frame = +1
Query: 172 LVVDNGSGMCKAGFAGDDAPRAVFPSIV 255
+++DNG+G K G+AG+DAP VFP+++
Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVI 35
Score = 28.7 bits (61), Expect = 0.63
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = +2
Query: 482 PQGQQREDEQIMFETXNTPAMYVAIQAV 565
P + +IMFE+ N +Y+A+QAV
Sbjct: 135 PPENRENTAEIMFESFNCAGLYIAVQAV 162
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 47.6 bits (108), Expect = 1e-06
Identities = 21/54 (38%), Positives = 30/54 (55%)
Frame = +3
Query: 342 LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREK 503
+ +K I +G V NWD +W + +L+ P EHP+L+TE NP NR K
Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVK 116
Score = 38.7 bits (86), Expect = 6e-04
Identities = 15/31 (48%), Positives = 23/31 (74%)
Frame = +1
Query: 157 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPS 249
+EV+A+V+D GS + GF+G+D P+ V PS
Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPS 39
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 43.6 bits (98), Expect = 2e-05
Identities = 22/74 (29%), Positives = 41/74 (55%), Gaps = 1/74 (1%)
Frame = +3
Query: 288 MGQKDSXVGDEAQSKRGILTL-KYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLL 464
+ + + VG++ + G ++ + P E +++NWD ME++ +TF +L + EHP+ +
Sbjct: 62 LSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICM 120
Query: 465 TEAPLNPKANREKM 506
TE NP R M
Sbjct: 121 TEPLANPTYVRSTM 134
Score = 33.1 bits (72), Expect = 0.029
Identities = 13/28 (46%), Positives = 20/28 (71%)
Frame = +1
Query: 172 LVVDNGSGMCKAGFAGDDAPRAVFPSIV 255
LV+DNGS +AG+ G+ P+ VF ++V
Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVFDNLV 54
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 41.1 bits (92), Expect = 1e-04
Identities = 22/84 (26%), Positives = 43/84 (51%), Gaps = 2/84 (2%)
Frame = +3
Query: 261 PRHQGVMVGMGQKDSXVGDEAQSKRGI--LTLKYPIEHGIVTNWDDMEKIWHHTFYNELR 434
P + GV + ++ V DE Q I + +K +GI+ +W+ W +L+
Sbjct: 37 PSYYGVRSDVTGRNKYVVDELQIHAPIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQ 96
Query: 435 VAPEEHPVLLTEAPLNPKANREKM 506
V P E+ +++TE NP++ R+++
Sbjct: 97 VNPTEYAMMITEPSWNPQSVRQQI 120
Score = 35.1 bits (77), Expect = 0.007
Identities = 13/31 (41%), Positives = 22/31 (70%)
Frame = +1
Query: 157 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPS 249
EE+ +LV+D GS + G+AG+++P + PS
Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPS 38
>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1666
Score = 27.1 bits (57), Expect = 1.9
Identities = 14/43 (32%), Positives = 22/43 (51%), Gaps = 2/43 (4%)
Frame = +3
Query: 417 FYNELRVAPEEHPVLLTE--APLNPKANREKMNRSCSKHXTRP 539
+Y L E+HP+LLT+ A L P+ + ++ R K P
Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTP 1441
>SPAC13F5.04c |||endosomal sorting protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 277
Score = 27.1 bits (57), Expect = 1.9
Identities = 17/66 (25%), Positives = 28/66 (42%)
Frame = -1
Query: 389 IPVSDDSVFDGVFEGQDTSFALCLISYXRVLLSHTDHHALMAGPSTIEGNTARGASSPAK 210
+P + +V +TSFAL S L A ++ +E + +S+PA
Sbjct: 63 LPTQEAAVETNASASHETSFALPTTSPAASLSISPTKSAAVSSEPNVEADVKSLSSTPAA 122
Query: 209 PALHIP 192
P L+ P
Sbjct: 123 PQLNSP 128
>SPAC22F8.08 |||COPII vesicle coat protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 926
Score = 25.8 bits (54), Expect = 4.4
Identities = 14/46 (30%), Positives = 23/46 (50%)
Frame = -1
Query: 296 LSHTDHHALMAGPSTIEGNTARGASSPAKPALHIPEPLSTTNAATS 159
+SHT H + P+ + T A+SPA P+L P + ++S
Sbjct: 83 VSHTSHKSRRQYPAEVFELTNTLAASPAPPSLSEPSYVMARTPSSS 128
>SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1828
Score = 25.8 bits (54), Expect = 4.4
Identities = 12/23 (52%), Positives = 14/23 (60%)
Frame = -2
Query: 199 TYRSHCLLPTRQLLRRTS*ISEI 131
TYR H +PT LL T +SEI
Sbjct: 168 TYRDHDAIPTATLLNPTLDVSEI 190
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 25.8 bits (54), Expect = 4.4
Identities = 7/21 (33%), Positives = 13/21 (61%)
Frame = +3
Query: 357 PIEHGIVTNWDDMEKIWHHTF 419
PI+ G V +W+ ++ W H +
Sbjct: 113 PIQRGRVVDWEALKAFWKHLY 133
>SPCP1E11.04c |pal1||membrane associated protein Pal1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 425
Score = 25.0 bits (52), Expect = 7.7
Identities = 14/33 (42%), Positives = 17/33 (51%)
Frame = -2
Query: 262 GLPRSRGTRREEHHLLRNRPCTYRSHCLLPTRQ 164
GL RS + L NRP YRSH + TR+
Sbjct: 307 GLNRSASMMSRTPNTL-NRPSNYRSHSSMGTRR 338
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,410,128
Number of Sequences: 5004
Number of extensions: 49617
Number of successful extensions: 146
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 126
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 144
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 240047038
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -