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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= e96h0318
         (629 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC577.09 |||ERCC-8 homolog |Schizosaccharomyces pombe|chr 2|||...    30   0.24 
SPBC16C6.13c |sec27||coatomer beta' subunit |Schizosaccharomyces...    29   0.55 
SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces po...    29   0.73 
SPCC16C4.08c |skb15||Shk1 kinase binding protein 15|Schizosaccha...    28   1.3  
SPAC17A2.10c |||sequence orphan|Schizosaccharomyces pombe|chr 1|...    27   1.7  
SPCC4G3.11 |mug154||conserved fungal protein|Schizosaccharomyces...    27   3.0  
SPCC1450.05c |rox3|med19|RNA polymerase II holoenzyme mediator c...    26   5.2  
SPBC1198.12 |mfr1|SPBC660.02|fizzy-related protein Mfr1|Schizosa...    26   5.2  
SPAC869.05c |||sulfate transporter |Schizosaccharomyces pombe|ch...    26   5.2  
SPAC3C7.07c |||arginine-tRNA protein transferase |Schizosaccharo...    25   9.0  
SPAC1687.05 |pli1||SUMO E3 ligase Pli1|Schizosaccharomyces pombe...    25   9.0  
SPCC645.11c |mug117||meiotically upregulated gene Mug117|Schizos...    25   9.0  
SPBC29A10.02 |spo5|mrb1, mug12, SPBC365.18|meiotic RNA-binding p...    25   9.0  

>SPBC577.09 |||ERCC-8 homolog |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 404

 Score = 30.3 bits (65), Expect = 0.24
 Identities = 20/92 (21%), Positives = 44/92 (47%), Gaps = 6/92 (6%)
 Frame = +3

Query: 237 EEMYPNGAADGTVKQWVITNIAQEFKINSTHSHEVHHNEKE-----EVTGVGY-SNEQFA 398
           E +  +G+ADGT + W I  ++  F     H+  +  N+        V G+ + S+ ++ 
Sbjct: 202 EFVLASGSADGTCRLWDIRKVSSSFACMDLHNKYLPSNQTNISHYGTVNGLAWTSDARYL 261

Query: 399 ITVGSFGTVVKWDLKSNVVKNYQHLLKSFKPV 494
            + G+   +  W+++S      ++ L+ F P+
Sbjct: 262 ASCGTDDRIRVWNMESG-----RNTLREFGPI 288


>SPBC16C6.13c |sec27||coatomer beta' subunit |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 796

 Score = 29.1 bits (62), Expect = 0.55
 Identities = 18/80 (22%), Positives = 36/80 (45%), Gaps = 3/80 (3%)
 Frame = +3

Query: 252 NGAADGTVKQW--VITNIAQEFKINSTHSHEVHHNEKEEVTGVGYSNEQFAITVGSFGTV 425
           +G+ DGTVK W  +  ++ + +  +   +  +  N+   +  VG+ N     ++G     
Sbjct: 245 SGSEDGTVKIWHTLSYSLIKSYNFSLDRAWCIAQNKDNGLVTVGFDNGLITFSLGRDEPS 304

Query: 426 VKWDLKSNVV-KNYQHLLKS 482
           V  D    VV  NY  ++ +
Sbjct: 305 VTMDSSGKVVWSNYNEVMSA 324


>SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 665

 Score = 28.7 bits (61), Expect = 0.73
 Identities = 13/38 (34%), Positives = 20/38 (52%)
 Frame = +3

Query: 288 ITNIAQEFKINSTHSHEVHHNEKEEVTGVGYSNEQFAI 401
           ITN A+   I S   H+ H    +E+T    SN+Q ++
Sbjct: 443 ITNSAKNEHIRSQRQHKTHAAPSKELTKESLSNDQLSV 480


>SPCC16C4.08c |skb15||Shk1 kinase binding protein
           15|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 341

 Score = 27.9 bits (59), Expect = 1.3
 Identities = 12/37 (32%), Positives = 22/37 (59%), Gaps = 1/37 (2%)
 Frame = +3

Query: 333 HEVHHNEKEEVTGVG-YSNEQFAITVGSFGTVVKWDL 440
           H +  +  + +TG+  + +E+ A+TVG  G +  WDL
Sbjct: 113 HTLKSSSHKGITGIAVHPSEKLALTVGGDGKLRLWDL 149


>SPAC17A2.10c |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 230

 Score = 27.5 bits (58), Expect = 1.7
 Identities = 17/66 (25%), Positives = 33/66 (50%)
 Frame = -1

Query: 488 LETFQQVLVIFNYI*FQIPFNNSSKRTNCYCKLFIAIPNTSYFFFFIMMHFMAVC*IYLE 309
           L  F+ +L+ F ++ F + F+ S        ++FI   ++     F+   F+ VC ++L 
Sbjct: 110 LSLFRCLLLFFFFLLFFLSFSFSFSFLFFLSQIFIVYFSSFPILHFLFFFFLCVC-VFLS 168

Query: 308 FLGYIS 291
           FL  +S
Sbjct: 169 FLFSLS 174


>SPCC4G3.11 |mug154||conserved fungal protein|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 316

 Score = 26.6 bits (56), Expect = 3.0
 Identities = 9/27 (33%), Positives = 18/27 (66%)
 Frame = -1

Query: 374 NTSYFFFFIMMHFMAVC*IYLEFLGYI 294
           NTS  +F   + F+++C +++ FL +I
Sbjct: 80  NTSRSWFRAFLSFLSICFLFISFLNFI 106


>SPCC1450.05c |rox3|med19|RNA polymerase II holoenzyme mediator
           complex subunit Med19 |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 138

 Score = 25.8 bits (54), Expect = 5.2
 Identities = 12/36 (33%), Positives = 17/36 (47%)
 Frame = +3

Query: 264 DGTVKQWVITNIAQEFKINSTHSHEVHHNEKEEVTG 371
           D T+KQW+   I +E  I+    H V   E   + G
Sbjct: 71  DNTIKQWLTNPIREEVPIDIEFLHHVFSVEPGIIPG 106


>SPBC1198.12 |mfr1|SPBC660.02|fizzy-related protein
           Mfr1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 421

 Score = 25.8 bits (54), Expect = 5.2
 Identities = 13/41 (31%), Positives = 24/41 (58%)
 Frame = +2

Query: 500 VLFSHLLLHVAVGTKQGVIFVLDLNGQGKTIYKVRGPDDEV 622
           VL++     +AVGT  GVI++ D+    K++  ++G  + V
Sbjct: 172 VLWTGKGTQLAVGTDSGVIYIWDIEST-KSVRSLKGHSERV 211


>SPAC869.05c |||sulfate transporter |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 840

 Score = 25.8 bits (54), Expect = 5.2
 Identities = 10/22 (45%), Positives = 15/22 (68%)
 Frame = +3

Query: 429 KWDLKSNVVKNYQHLLKSFKPV 494
           K ++ SN+ KN+ H LKS  P+
Sbjct: 88  KKNVTSNIFKNFLHYLKSLFPI 109


>SPAC3C7.07c |||arginine-tRNA protein transferase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 361

 Score = 25.0 bits (52), Expect = 9.0
 Identities = 12/43 (27%), Positives = 23/43 (53%)
 Frame = +3

Query: 255 GAADGTVKQWVITNIAQEFKINSTHSHEVHHNEKEEVTGVGYS 383
           GA   +V     T   ++F++   +  +VH  ++EE+T  G+S
Sbjct: 93  GAEKYSVTMEPCTYTDEKFEVFKKYQMQVHLEKEEEITKKGFS 135


>SPAC1687.05 |pli1||SUMO E3 ligase Pli1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 727

 Score = 25.0 bits (52), Expect = 9.0
 Identities = 9/25 (36%), Positives = 14/25 (56%)
 Frame = -2

Query: 442 FRSHLTTVPKEPTVIANCSLLYPTP 368
           +R +L + P E     NC + +PTP
Sbjct: 171 YRVYLFSTPSETIGFGNCLMEFPTP 195


>SPCC645.11c |mug117||meiotically upregulated gene
           Mug117|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 186

 Score = 25.0 bits (52), Expect = 9.0
 Identities = 9/20 (45%), Positives = 12/20 (60%)
 Frame = -1

Query: 431 FNNSSKRTNCYCKLFIAIPN 372
           FNN ++ T CY KL    P+
Sbjct: 147 FNNDNENTRCYVKLNYCYPD 166


>SPBC29A10.02 |spo5|mrb1, mug12, SPBC365.18|meiotic RNA-binding
           protein 1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 567

 Score = 25.0 bits (52), Expect = 9.0
 Identities = 12/37 (32%), Positives = 21/37 (56%)
 Frame = +3

Query: 282 WVITNIAQEFKINSTHSHEVHHNEKEEVTGVGYSNEQ 392
           W  ++I+  FK +   S+ V  + KE+  GVG++  Q
Sbjct: 395 WNESDISTLFKPSKIISNRVLRDSKEQSRGVGFARMQ 431


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,738,620
Number of Sequences: 5004
Number of extensions: 59287
Number of successful extensions: 194
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 181
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 194
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 279695522
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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