BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e96h0315 (794 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon domain-con... 53 2e-07 At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon domain-con... 50 2e-06 At5g65990.1 68418.m08308 amino acid transporter family protein s... 31 1.2 At2g44410.1 68415.m05523 expressed protein 29 2.7 At5g60710.1 68418.m07618 zinc finger (C3HC4-type RING finger) fa... 29 4.7 >At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon domain-containing protein low similarity to SP|Q13144 Translation initiation factor eIF-2B epsilon subunit (eIF-2B GDP-GTP exchange factor) {Homo sapiens}; contains Pfam profile PF02020: eIF4-gamma/eIF5/eIF2-epsilon Length = 411 Score = 53.2 bits (122), Expect = 2e-07 Identities = 30/88 (34%), Positives = 47/88 (53%) Frame = +2 Query: 194 SEGRKTSIIGSTDQDQKKR*EEKYDPNGFRDALVQGLERAGGDLDAAYKYLDSAGSKLDY 373 S K ++ G+ + +K+ DP F DA+VQ GDL+ K ++S S L++ Sbjct: 2 SSKEKPTLGGTRIKTRKRNIAAPLDPAAFSDAVVQIYHDNAGDLELVAKSIES--SDLNF 59 Query: 374 RRYGEVIFDVLIAGGLLLPGGSVSMDGE 457 RYG++ F+V+ GG PG S +GE Sbjct: 60 TRYGDIFFEVIFIGGRTQPGTVKSDEGE 87 >At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon domain-containing protein low similarity to SP|P47823 Translation initiation factor eIF-2B epsilon subunit (eIF-2B GDP-GTP exchange factor) {Oryctolagus cuniculus}; contains Pfam profile PF02020: eIF4-gamma/eIF5/eIF2-epsilon Length = 411 Score = 50.0 bits (114), Expect = 2e-06 Identities = 30/88 (34%), Positives = 46/88 (52%) Frame = +2 Query: 194 SEGRKTSIIGSTDQDQKKR*EEKYDPNGFRDALVQGLERAGGDLDAAYKYLDSAGSKLDY 373 S K ++ G+ + +K+ DP F DALVQ GDL+ + L+S S L++ Sbjct: 2 SSKEKPTLGGTRIKTRKRNIAAPLDPAAFSDALVQIYLDNAGDLELVARSLES--SDLNF 59 Query: 374 RRYGEVIFDVLIAGGLLLPGGSVSMDGE 457 RYG++ F+V+ GG G S +GE Sbjct: 60 SRYGDIFFEVVFIGGRTQTGSVKSDEGE 87 >At5g65990.1 68418.m08308 amino acid transporter family protein similar to proton/amino acid transporter 1 [Mus musculus] GI:21908024; contains Pfam profile PF01490: Transmembrane amino acid transporter protein Length = 427 Score = 30.7 bits (66), Expect = 1.2 Identities = 17/42 (40%), Positives = 25/42 (59%), Gaps = 3/42 (7%) Frame = -2 Query: 700 DQQHGWSTHPLPIPQCSHA---RQLDALFGNRSLSSRPEPFS 584 DQ+ G S+H LP SH+ + L G R+LSS+P+ F+ Sbjct: 4 DQEAGSSSHSLPSGSSSHSLPPTEDTPLLGPRTLSSQPKTFA 45 >At2g44410.1 68415.m05523 expressed protein Length = 413 Score = 29.5 bits (63), Expect = 2.7 Identities = 15/33 (45%), Positives = 19/33 (57%) Frame = -1 Query: 341 TCRLRRDRHRPAPDPVPERRGNRSGHTSLLISF 243 T LRR+R RP+P + NRS + S ISF Sbjct: 319 TTSLRRNRRRPSPAVRASYQRNRSNNASQTISF 351 >At5g60710.1 68418.m07618 zinc finger (C3HC4-type RING finger) family protein low similarity to SP|P97280 Inter-alpha-trypsin inhibitor heavy chain H3 precursor {Mesocricetus auratus}; contains Pfam profiles PF00097: Zinc finger, C3HC4 type (RING finger), PF00092: von Willebrand factor type A domain Length = 704 Score = 28.7 bits (61), Expect = 4.7 Identities = 15/50 (30%), Positives = 26/50 (52%) Frame = +3 Query: 489 PMRIWTPAKFRTGIRETDASVQVLGKNVRRGNEKGSGLLERLRFPNSASS 638 P+R+ T + ++ ++ V G N+ G +KG+ +L RF N SS Sbjct: 302 PLRLMTETGKQEALQAVNSLVSNGGTNIAEGLKKGARVLIDRRFKNPVSS 351 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 18,188,052 Number of Sequences: 28952 Number of extensions: 410814 Number of successful extensions: 1436 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 1327 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1432 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 1794809600 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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