Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= e96h0307
         (695 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC2C4.16c |rps801|rps8-1|40S ribosomal protein S8|Schizosaccha...   132   5e-32
SPAC521.05 |rps802|rps8-2|40S ribosomal protein S8|Schizosacchar...   131   8e-32

>SPAC2C4.16c |rps801|rps8-1|40S ribosomal protein
           S8|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 200

 Score =  132 bits (319), Expect = 5e-32
 Identities = 81/197 (41%), Positives = 104/197 (52%)
 Frame = +3

Query: 69  RDXWHXXRATGGKRAPIRKKRXYELGRPAAKHQARPSANPLRSFTWWXY*VPCXASGHR* 248
           RD  H   ATG KRA  RKKR +ELGR  +  +  P                        
Sbjct: 5   RDSRHKRSATGAKRAQYRKKRKFELGRQPSNTRIGPKRIHEVRVRGGNKKFRALRLDSGN 64

Query: 249 LLWGSXXSTRKTRIIDVVXNASNNXLVRTKTLVKNAIVVVDATPFRQWYESHYTLPLGRX 428
             WGS   ++KTRII V  + SNN LVRT TL K+AIV +DA PFR WYE+HY + +G  
Sbjct: 65  FSWGSEGVSKKTRIIQVAYHPSNNELVRTNTLTKSAIVQIDAAPFRVWYETHYGILMG-S 123

Query: 429 KGAKLTEAEEAIINKKRSQKTARKYLAGNVLLRLRVL*KSNSTQGVCWACVASRPGQCGR 608
           KG K T          +S+   RK+ A     ++    ++    G  +A V+SRPGQ GR
Sbjct: 124 KGKKATSTP-----NPKSKHVQRKHSARLGDSKVDSALETQFAAGRLYAVVSSRPGQSGR 178

Query: 609 ADGYILEGKELEFYLKK 659
            DGYILEG+EL FYL++
Sbjct: 179 CDGYILEGEELHFYLRR 195



 Score = 57.2 bits (132), Expect = 2e-09
 Identities = 26/46 (56%), Positives = 32/46 (69%)
 Frame = +1

Query: 118 YARRGSMS*GVPLPNTRLGPQRIHSVRSRGGXTKYRALRLDTGNFS 255
           Y ++     G    NTR+GP+RIH VR RGG  K+RALRLD+GNFS
Sbjct: 21  YRKKRKFELGRQPSNTRIGPKRIHEVRVRGGNKKFRALRLDSGNFS 66


>SPAC521.05 |rps802|rps8-2|40S ribosomal protein
           S8|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 200

 Score =  131 bits (317), Expect = 8e-32
 Identities = 81/197 (41%), Positives = 104/197 (52%)
 Frame = +3

Query: 69  RDXWHXXRATGGKRAPIRKKRXYELGRPAAKHQARPSANPLRSFTWWXY*VPCXASGHR* 248
           RD  H   ATG KRA  RKKR +ELGR  +  +  P                        
Sbjct: 5   RDSRHKRSATGAKRAQYRKKRKFELGRQPSNTRIGPKRIHEVRVRGGNKKFRALRLDSGN 64

Query: 249 LLWGSXXSTRKTRIIDVVXNASNNXLVRTKTLVKNAIVVVDATPFRQWYESHYTLPLGRX 428
             WGS   ++KTRII V  + SNN LVRT TL K+AIV +DA PFR WYE+HY + +G  
Sbjct: 65  FSWGSEGVSKKTRIIQVAYHPSNNELVRTNTLTKSAIVQIDAAPFRVWYETHYGILMG-S 123

Query: 429 KGAKLTEAEEAIINKKRSQKTARKYLAGNVLLRLRVL*KSNSTQGVCWACVASRPGQCGR 608
           KG K T          +S+   RK+ A     ++    ++    G  +A V+SRPGQ GR
Sbjct: 124 KGKKATATP-----TPKSKHVQRKHSARLGDSKVDSALETQFAAGRLYAVVSSRPGQSGR 178

Query: 609 ADGYILEGKELEFYLKK 659
            DGYILEG+EL FYL++
Sbjct: 179 CDGYILEGEELHFYLRR 195



 Score = 57.2 bits (132), Expect = 2e-09
 Identities = 26/46 (56%), Positives = 32/46 (69%)
 Frame = +1

Query: 118 YARRGSMS*GVPLPNTRLGPQRIHSVRSRGGXTKYRALRLDTGNFS 255
           Y ++     G    NTR+GP+RIH VR RGG  K+RALRLD+GNFS
Sbjct: 21  YRKKRKFELGRQPSNTRIGPKRIHEVRVRGGNKKFRALRLDSGNFS 66


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,424,519
Number of Sequences: 5004
Number of extensions: 43196
Number of successful extensions: 111
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 98
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 107
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 321151040
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)