Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0298
(734 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC736.03c |||phenylalanyl-tRNA synthetase|Schizosaccharomyces ... 82 7e-17
SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2 |... 31 0.23
SPAC3H8.05c |||conserved fungal protein|Schizosaccharomyces pomb... 28 1.6
SPCC757.11c |||membrane transporter|Schizosaccharomyces pombe|ch... 27 3.7
SPAC29A4.03c |||mitochondrial ribosomal protein subunit S9|Schiz... 27 3.7
SPBC947.12 |kms2||spindle pole body protein Kms2|Schizosaccharom... 26 4.8
SPAC3A12.05c |taf2||TATA-binding protein associated factor Taf2|... 26 6.4
SPAC24B11.11c |sid2||Sid2p-Mob1p kinase complex|Schizosaccharomy... 25 8.5
SPAC4F10.09c |||ribosome biogenesis protein Noc1 |Schizosaccharo... 25 8.5
SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr 3|... 25 8.5
SPAC144.15c |cog1||Golgi transport complex subunit Cog1 |Schizos... 25 8.5
SPAC3C7.01c ||SPAC732.03c|inositol polyphosphate phosphatase |Sc... 25 8.5
SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr... 25 8.5
>SPCC736.03c |||phenylalanyl-tRNA synthetase|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 429
Score = 82.2 bits (194), Expect = 7e-17
Identities = 40/94 (42%), Positives = 56/94 (59%), Gaps = 6/94 (6%)
Frame = +2
Query: 386 SPVVSTKQNFDDLLIPNDHPSRLKSDCYYINKDTLLRAHMT-HIKPNY*DLGLD-----N 547
SP+VS + NFD L P H SR KSD YY+N T LR H + H + L +
Sbjct: 75 SPIVSVETNFDSLGFPKTHVSRSKSDTYYMNNKTCLRTHTSAHQPEEFSRLAKEGFKKNG 134
Query: 548 FLMIGDVYRRDEIDSTHFPVFHQVDAVRLQRKEE 649
FL+ DVYRRDE+DS+H+P+FHQ++ + + +
Sbjct: 135 FLITADVYRRDEVDSSHYPIFHQMEGALVWNRND 168
>SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 499
Score = 30.7 bits (66), Expect = 0.23
Identities = 12/31 (38%), Positives = 18/31 (58%)
Frame = +2
Query: 548 FLMIGDVYRRDEIDSTHFPVFHQVDAVRLQR 640
+ I V+R + +D+TH FHQV+ V R
Sbjct: 350 YFSIDRVFRNETVDATHLAEFHQVEGVICDR 380
>SPAC3H8.05c |||conserved fungal protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1073
Score = 27.9 bits (59), Expect = 1.6
Identities = 15/44 (34%), Positives = 25/44 (56%)
Frame = +2
Query: 362 TFSVYDNLSPVVSTKQNFDDLLIPNDHPSRLKSDCYYINKDTLL 493
T S+ D+++ +N L+ +D SRL DC+YI++ LL
Sbjct: 937 TSSLRDSVAVFQYDSENNSLNLVCSDTSSRLGIDCFYISQKKLL 980
>SPCC757.11c |||membrane transporter|Schizosaccharomyces pombe|chr
3|||Manual
Length = 471
Score = 26.6 bits (56), Expect = 3.7
Identities = 14/64 (21%), Positives = 30/64 (46%), Gaps = 3/64 (4%)
Frame = +1
Query: 100 MTKLLFRTNRAIAIQVLSRYKSNAAP---QTLTTVRIKDKDHITDEYTNVTPKILLIWVA 270
+ + +RT+ + V++ A P + +K + I +EYT+ + IWV
Sbjct: 175 LISIKYRTSMGVVNSVMATLGLTAGPFIGGLMAKSSMKSQSDIWNEYTSGNWLMAFIWVG 234
Query: 271 IYIF 282
+++F
Sbjct: 235 LFLF 238
>SPAC29A4.03c |||mitochondrial ribosomal protein subunit
S9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 132
Score = 26.6 bits (56), Expect = 3.7
Identities = 14/33 (42%), Positives = 19/33 (57%)
Frame = -2
Query: 100 FYVNLVEVPNDILFLLTIHMKHSIY*PVFTGNR 2
FYVN P D+ F +H KH++Y P+ NR
Sbjct: 30 FYVN--GSPFDVYFQRMVHRKHAVY-PLAACNR 59
>SPBC947.12 |kms2||spindle pole body protein
Kms2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 457
Score = 26.2 bits (55), Expect = 4.8
Identities = 15/35 (42%), Positives = 21/35 (60%)
Frame = +1
Query: 145 VLSRYKSNAAPQTLTTVRIKDKDHITDEYTNVTPK 249
VL R KS A PQ ++ RIK KD + + +TP+
Sbjct: 98 VLPR-KSVAKPQKISENRIKRKDMFYQDASYITPR 131
>SPAC3A12.05c |taf2||TATA-binding protein associated factor
Taf2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1174
Score = 25.8 bits (54), Expect = 6.4
Identities = 24/77 (31%), Positives = 33/77 (42%)
Frame = +2
Query: 245 LKYFLFGSQSTSSKRKSFINRATGIVEYFYRRFTHRGKPTFSVYDNLSPVVSTKQNFDDL 424
L+ F F ST K F N I EY+ + P ++P S K+ DL
Sbjct: 800 LEKFCF-KDSTIPKSNDFSN----ITEYYVKCAMLESFPNIRDRKGVTPS-SVKKLLLDL 853
Query: 425 LIPNDHPSRLKSDCYYI 475
L ND+ + SD Y+I
Sbjct: 854 LCYNDNANNEFSDAYFI 870
>SPAC24B11.11c |sid2||Sid2p-Mob1p kinase complex|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 607
Score = 25.4 bits (53), Expect = 8.5
Identities = 11/24 (45%), Positives = 17/24 (70%)
Frame = +2
Query: 383 LSPVVSTKQNFDDLLIPNDHPSRL 454
LSPV S+K+N+ D + + H S+L
Sbjct: 59 LSPVASSKENYPDNHMHSKHISKL 82
>SPAC4F10.09c |||ribosome biogenesis protein Noc1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 860
Score = 25.4 bits (53), Expect = 8.5
Identities = 10/22 (45%), Positives = 14/22 (63%)
Frame = +2
Query: 356 KPTFSVYDNLSPVVSTKQNFDD 421
KP FS +NLS + ++ FDD
Sbjct: 779 KPMFSDEENLSEIAHSEDEFDD 800
>SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr
3|||Manual
Length = 531
Score = 25.4 bits (53), Expect = 8.5
Identities = 10/30 (33%), Positives = 17/30 (56%)
Frame = +2
Query: 536 GLDNFLMIGDVYRRDEIDSTHFPVFHQVDA 625
G++ +G +R + ID+TH P F +A
Sbjct: 270 GMNRVFELGKNFRNEGIDATHNPEFTSCEA 299
>SPAC144.15c |cog1||Golgi transport complex subunit Cog1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 701
Score = 25.4 bits (53), Expect = 8.5
Identities = 24/71 (33%), Positives = 32/71 (45%), Gaps = 1/71 (1%)
Frame = +2
Query: 392 VVSTKQNFDDLLIPNDHPSRLKSDCYYINKDTLLRAH-MTHIKPNY*DLGLDNFLMIGDV 568
+VS K F LL PN L + +N + L A M H+K N D+ N+L
Sbjct: 589 IVSDK--FKGLLTPN-----LFESLFILNLERLKAAKSMDHMKQNTFDMEFINYLSDFTN 641
Query: 569 YRRDEIDSTHF 601
R+ ID HF
Sbjct: 642 NPREVIDYQHF 652
>SPAC3C7.01c ||SPAC732.03c|inositol polyphosphate phosphatase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 611
Score = 25.4 bits (53), Expect = 8.5
Identities = 14/45 (31%), Positives = 23/45 (51%)
Frame = +1
Query: 220 TDEYTNVTPKILLIWVAIYIFKKEILYQSCDRNRGIFLSQVYSQR 354
T +Y NV L ++ + I+K +L SCD + I + +Y R
Sbjct: 43 TPDYNNVQ---LYGFIRLKIYKYVVLVTSCDLHAAILGNNIYRAR 84
>SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 591
Score = 25.4 bits (53), Expect = 8.5
Identities = 11/29 (37%), Positives = 15/29 (51%)
Frame = +2
Query: 536 GLDNFLMIGDVYRRDEIDSTHFPVFHQVD 622
GLD IG +R + D TH P F ++
Sbjct: 315 GLDRVYEIGRQFRNEGADLTHNPEFTSIE 343
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,122,442
Number of Sequences: 5004
Number of extensions: 67613
Number of successful extensions: 163
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 157
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 162
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 347244562
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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