Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0282
(653 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q3V4A0 Cluster: 18-day embryo whole body cDNA, RIKEN fu... 131 2e-29
UniRef50_Q9UM00 Cluster: Transmembrane and coiled-coil domain-co... 131 2e-29
UniRef50_UPI0000EAFFFA Cluster: UPI0000EAFFFA related cluster; n... 125 8e-28
UniRef50_Q54TU8 Cluster: Putative uncharacterized protein; n=1; ... 92 1e-17
UniRef50_Q9FG04 Cluster: Dbj|BAA86974.1; n=9; Magnoliophyta|Rep:... 80 4e-14
UniRef50_Q23FA1 Cluster: Putative uncharacterized protein; n=1; ... 78 2e-13
UniRef50_A0D1Y0 Cluster: Chromosome undetermined scaffold_342, w... 58 1e-07
UniRef50_UPI000049893D Cluster: conserved hypothetical protein; ... 57 3e-07
UniRef50_Q4UBZ7 Cluster: Putative uncharacterized protein; n=2; ... 47 5e-04
UniRef50_Q5CMV1 Cluster: Dbj|baa86974.1; n=3; Cryptosporidium|Re... 45 0.001
UniRef50_Q7RQW5 Cluster: Arabidopsis thaliana At5g06660/F15M7_19... 44 0.002
UniRef50_A2F9D9 Cluster: Putative uncharacterized protein; n=1; ... 43 0.007
UniRef50_A2D7Z5 Cluster: Putative uncharacterized protein; n=1; ... 38 0.16
UniRef50_A1ZHT1 Cluster: Major facilitator family transporter, p... 33 4.5
>UniRef50_Q3V4A0 Cluster: 18-day embryo whole body cDNA, RIKEN
full-length enriched library, clone:1190006A08
product:Membrane protein homolog; n=19; Eumetazoa|Rep:
18-day embryo whole body cDNA, RIKEN full-length
enriched library, clone:1190006A08 product:Membrane
protein homolog - Mus musculus (Mouse)
Length = 200
Score = 131 bits (316), Expect = 2e-29
Identities = 65/100 (65%), Positives = 74/100 (74%)
Frame = +3
Query: 306 DLSLVKMKSMFAIGFAFTALLSMFNSIFDGRVVAKLPFYPISWIQGLSHRNLPGDDYTDC 485
DLS+V+MKSMFAIGF FTAL+ MFNSIFDGRVVAKLPF P+S+IQGLSHRNL GDD TDC
Sbjct: 94 DLSMVRMKSMFAIGFCFTALMGMFNSIFDGRVVAKLPFTPLSYIQGLSHRNLLGDDTTDC 153
Query: 486 SFIFLYILVQ*ASDKTYKSCLVLHLLELASKQGGALFAAP 605
SFIFLYIL + + + L L A+KQ G P
Sbjct: 154 SFIFLYILCTMSIRQNIQKILGLAPSRAATKQAGGFLGPP 193
Score = 62.9 bits (146), Expect = 6e-09
Identities = 27/38 (71%), Positives = 34/38 (89%)
Frame = +1
Query: 130 EGLTWVLVYRTEKYQKLKVEVERQSKKLEKRKEAHGDS 243
EG+TWVLVYRT+KY++LK EVE+QSKKLEK+KE +S
Sbjct: 35 EGITWVLVYRTDKYKRLKAEVEKQSKKLEKKKETITES 72
>UniRef50_Q9UM00 Cluster: Transmembrane and coiled-coil
domain-containing protein 1; n=18; Euteleostomi|Rep:
Transmembrane and coiled-coil domain-containing protein
1 - Homo sapiens (Human)
Length = 188
Score = 131 bits (316), Expect = 2e-29
Identities = 65/100 (65%), Positives = 74/100 (74%)
Frame = +3
Query: 306 DLSLVKMKSMFAIGFAFTALLSMFNSIFDGRVVAKLPFYPISWIQGLSHRNLPGDDYTDC 485
DLS+V+MKSMFAIGF FTAL+ MFNSIFDGRVVAKLPF P+S+IQGLSHRNL GDD TDC
Sbjct: 82 DLSMVRMKSMFAIGFCFTALMGMFNSIFDGRVVAKLPFTPLSYIQGLSHRNLLGDDTTDC 141
Query: 486 SFIFLYILVQ*ASDKTYKSCLVLHLLELASKQGGALFAAP 605
SFIFLYIL + + + L L A+KQ G P
Sbjct: 142 SFIFLYILCTMSIRQNIQKILGLAPSRAATKQAGGFLGPP 181
Score = 90.6 bits (215), Expect = 3e-17
Identities = 40/57 (70%), Positives = 51/57 (89%)
Frame = +1
Query: 73 MWSDSLLIVFISICTAFLGEGLTWVLVYRTEKYQKLKVEVERQSKKLEKRKEAHGDS 243
M++D+LLIVFIS+CTA L EG+TWVLVYRT+KY++LK EVE+QSKKLEK+KE +S
Sbjct: 4 MFADTLLIVFISVCTALLAEGITWVLVYRTDKYKRLKAEVEKQSKKLEKKKETITES 60
>UniRef50_UPI0000EAFFFA Cluster: UPI0000EAFFFA related cluster; n=1;
Canis lupus familiaris|Rep: UPI0000EAFFFA UniRef100
entry - Canis familiaris
Length = 241
Score = 125 bits (302), Expect = 8e-28
Identities = 63/86 (73%), Positives = 70/86 (81%), Gaps = 1/86 (1%)
Frame = +3
Query: 306 DLSLVKMKSMFAIGFAFTALLSMFNSIFDGRVVAKLPFYPISWIQGLSHRNLPGDDYTDC 485
DLS+V+MKSMFAIGF FTAL+ MFNSIFDGRVVAKLPF P+S+IQGLSHRNL GDD TDC
Sbjct: 82 DLSMVRMKSMFAIGFCFTALMGMFNSIFDGRVVAKLPFTPLSYIQGLSHRNLLGDDTTDC 141
Query: 486 SFIFLYIL-VQ*ASDKTYKSCLVLHL 560
SFIFLYIL +T + LVL L
Sbjct: 142 SFIFLYILCTMSIRQRTSRRFLVLPL 167
Score = 90.6 bits (215), Expect = 3e-17
Identities = 40/57 (70%), Positives = 51/57 (89%)
Frame = +1
Query: 73 MWSDSLLIVFISICTAFLGEGLTWVLVYRTEKYQKLKVEVERQSKKLEKRKEAHGDS 243
M++D+LLIVFIS+CTA L EG+TWVLVYRT+KY++LK EVE+QSKKLEK+KE +S
Sbjct: 4 MFADTLLIVFISVCTALLAEGITWVLVYRTDKYKRLKAEVEKQSKKLEKKKETITES 60
>UniRef50_Q54TU8 Cluster: Putative uncharacterized protein; n=1;
Dictyostelium discoideum AX4|Rep: Putative
uncharacterized protein - Dictyostelium discoideum AX4
Length = 186
Score = 91.9 bits (218), Expect = 1e-17
Identities = 40/68 (58%), Positives = 49/68 (72%)
Frame = +3
Query: 306 DLSLVKMKSMFAIGFAFTALLSMFNSIFDGRVVAKLPFYPISWIQGLSHRNLPGDDYTDC 485
+LS KMKSMFA+ + AL S N IFDG VV KLPF PI ++QG+SHR + GDDYTDC
Sbjct: 84 ELSFSKMKSMFAVAISMIALFSYLNRIFDGVVVCKLPFVPIGFLQGISHRTIAGDDYTDC 143
Query: 486 SFIFLYIL 509
S F+Y +
Sbjct: 144 SMTFIYAI 151
Score = 48.4 bits (110), Expect = 1e-04
Identities = 19/44 (43%), Positives = 32/44 (72%)
Frame = +1
Query: 88 LLIVFISICTAFLGEGLTWVLVYRTEKYQKLKVEVERQSKKLEK 219
L I+F+SI ++ EG++W+LVYRTE Y++ K ++R +L+K
Sbjct: 7 LFILFVSIASSLASEGVSWLLVYRTENYKRGKANIDRLQIQLDK 50
>UniRef50_Q9FG04 Cluster: Dbj|BAA86974.1; n=9; Magnoliophyta|Rep:
Dbj|BAA86974.1 - Arabidopsis thaliana (Mouse-ear cress)
Length = 196
Score = 80.2 bits (189), Expect = 4e-14
Identities = 35/68 (51%), Positives = 47/68 (69%)
Frame = +3
Query: 306 DLSLVKMKSMFAIGFAFTALLSMFNSIFDGRVVAKLPFYPISWIQGLSHRNLPGDDYTDC 485
DLSL K KS + + + NS+F+G+VVAKLPF+PI+ ++ +SHR L GDD TDC
Sbjct: 93 DLSLFKFKSGAVVALVLFVVFGLLNSLFEGKVVAKLPFHPITIVRKMSHRGLKGDDSTDC 152
Query: 486 SFIFLYIL 509
S FLY+L
Sbjct: 153 SMAFLYLL 160
Score = 59.3 bits (137), Expect = 8e-08
Identities = 24/50 (48%), Positives = 36/50 (72%)
Frame = +1
Query: 76 WSDSLLIVFISICTAFLGEGLTWVLVYRTEKYQKLKVEVERQSKKLEKRK 225
++DSL +V IS CTA + E ++W+L+YRT Y+ LK +++ SKKLE K
Sbjct: 13 YADSLTVVGISFCTALVCEAISWILIYRTSSYKSLKSSIDKASKKLETMK 62
>UniRef50_Q23FA1 Cluster: Putative uncharacterized protein; n=1;
Tetrahymena thermophila SB210|Rep: Putative
uncharacterized protein - Tetrahymena thermophila SB210
Length = 432
Score = 77.8 bits (183), Expect = 2e-13
Identities = 34/69 (49%), Positives = 47/69 (68%)
Frame = +3
Query: 306 DLSLVKMKSMFAIGFAFTALLSMFNSIFDGRVVAKLPFYPISWIQGLSHRNLPGDDYTDC 485
++S KMKS F I L S+F G +VAKLPF PIS+I GL+HR L G+D++DC
Sbjct: 156 EMSSFKMKSTFLIAIFMIGALYTIGSLFSGLIVAKLPFAPISFITGLTHRGLSGEDFSDC 215
Query: 486 SFIFLYILV 512
++IF+Y+ V
Sbjct: 216 AYIFIYVQV 224
Score = 44.8 bits (101), Expect = 0.002
Identities = 18/48 (37%), Positives = 30/48 (62%)
Frame = +1
Query: 91 LIVFISICTAFLGEGLTWVLVYRTEKYQKLKVEVERQSKKLEKRKEAH 234
L+V +I T+ + E TW++VYR ++Y+ LK +E KL K +E +
Sbjct: 83 LVVVCAIATSSIAEFATWLIVYRRDEYKTLKQNIENSQNKLNKAQEVY 130
>UniRef50_A0D1Y0 Cluster: Chromosome undetermined scaffold_342,
whole genome shotgun sequence; n=2; Paramecium
tetraurelia|Rep: Chromosome undetermined scaffold_342,
whole genome shotgun sequence - Paramecium tetraurelia
Length = 184
Score = 58.4 bits (135), Expect = 1e-07
Identities = 27/68 (39%), Positives = 41/68 (60%)
Frame = +3
Query: 306 DLSLVKMKSMFAIGFAFTALLSMFNSIFDGRVVAKLPFYPISWIQGLSHRNLPGDDYTDC 485
+++ KM S A+ ++ + + G VVAKL F P +Q ++HR L G+D TDC
Sbjct: 83 EMTFKKMISNAAVAILSIVTINSIGNYYSGIVVAKLAFEPFWILQQITHRGLNGEDLTDC 142
Query: 486 SFIFLYIL 509
S+IF+YIL
Sbjct: 143 SYIFIYIL 150
Score = 33.9 bits (74), Expect = 3.4
Identities = 16/50 (32%), Positives = 27/50 (54%)
Frame = +1
Query: 82 DSLLIVFISICTAFLGEGLTWVLVYRTEKYQKLKVEVERQSKKLEKRKEA 231
++L I+ + + +GE ++W LVYR Y+ L +E K K KE+
Sbjct: 7 ETLFIIVGAAISISVGELVSWFLVYRNADYKDLVSRIETAVTKYNKEKES 56
>UniRef50_UPI000049893D Cluster: conserved hypothetical protein;
n=1; Entamoeba histolytica HM-1:IMSS|Rep: conserved
hypothetical protein - Entamoeba histolytica HM-1:IMSS
Length = 176
Score = 57.2 bits (132), Expect = 3e-07
Identities = 24/46 (52%), Positives = 31/46 (67%)
Frame = +3
Query: 378 NSIFDGRVVAKLPFYPISWIQGLSHRNLPGDDYTDCSFIFLYILVQ 515
N +F V KLPF P S+ ++HRNLPGDD TDCS F Y+L++
Sbjct: 96 NYMFKPVKVGKLPFQPFSFFTKVTHRNLPGDDMTDCSLFFFYVLMR 141
>UniRef50_Q4UBZ7 Cluster: Putative uncharacterized protein; n=2;
Theileria|Rep: Putative uncharacterized protein -
Theileria annulata
Length = 203
Score = 46.8 bits (106), Expect = 5e-04
Identities = 22/61 (36%), Positives = 33/61 (54%)
Frame = +3
Query: 330 SMFAIGFAFTALLSMFNSIFDGRVVAKLPFYPISWIQGLSHRNLPGDDYTDCSFIFLYIL 509
S F G L+ S+F+ VVAKLPF P+ + +H L G+D TDC+ +Y +
Sbjct: 98 STFITGLLLMGLMPAVMSVFEYSVVAKLPFTPLPPLSMFTHSKLLGNDITDCTATAVYTV 157
Query: 510 V 512
+
Sbjct: 158 I 158
Score = 33.5 bits (73), Expect = 4.5
Identities = 11/32 (34%), Positives = 22/32 (68%)
Frame = +1
Query: 82 DSLLIVFISICTAFLGEGLTWVLVYRTEKYQK 177
D++LI+ + + L E ++W+ +YR EK++K
Sbjct: 14 DTVLILLFGVISGVLNEVISWLFIYRKEKFKK 45
>UniRef50_Q5CMV1 Cluster: Dbj|baa86974.1; n=3; Cryptosporidium|Rep:
Dbj|baa86974.1 - Cryptosporidium hominis
Length = 195
Score = 45.2 bits (102), Expect = 0.001
Identities = 21/65 (32%), Positives = 34/65 (52%)
Frame = +3
Query: 309 LSLVKMKSMFAIGFAFTALLSMFNSIFDGRVVAKLPFYPISWIQGLSHRNLPGDDYTDCS 488
+S++KMKS +G F AL+ + N+ F + KLPF PI + ++ L D
Sbjct: 105 ISMIKMKSGILVGILFAALIPLKNTFFGDLPICKLPFEPIKIFRLVTQAGLKNASVDDAG 164
Query: 489 FIFLY 503
IF++
Sbjct: 165 SIFIF 169
>UniRef50_Q7RQW5 Cluster: Arabidopsis thaliana At5g06660/F15M7_19,
putative; n=5; Plasmodium|Rep: Arabidopsis thaliana
At5g06660/F15M7_19, putative - Plasmodium yoelii yoelii
Length = 218
Score = 44.4 bits (100), Expect = 0.002
Identities = 21/68 (30%), Positives = 36/68 (52%)
Frame = +3
Query: 309 LSLVKMKSMFAIGFAFTALLSMFNSIFDGRVVAKLPFYPISWIQGLSHRNLPGDDYTDCS 488
++ +K KS G F +++ + S+F+G +A LPF PI ++ L G + CS
Sbjct: 91 MTALKTKSNIITGLIFLSIMPLLFSLFEGLTIAILPFRPIFPFTLITQTGLQGKNIYHCS 150
Query: 489 FIFLYILV 512
F+Y L+
Sbjct: 151 STFIYTLI 158
Score = 39.1 bits (87), Expect = 0.091
Identities = 15/50 (30%), Positives = 30/50 (60%)
Frame = +1
Query: 79 SDSLLIVFISICTAFLGEGLTWVLVYRTEKYQKLKVEVERQSKKLEKRKE 228
+D +++ I++ E L+W+ VYR EK+ L E++ ++++K KE
Sbjct: 9 TDIFIVIGIAVACGIFSEFLSWIFVYRNEKFITLNEELKSLYEQVQKEKE 58
>UniRef50_A2F9D9 Cluster: Putative uncharacterized protein; n=1;
Trichomonas vaginalis G3|Rep: Putative uncharacterized
protein - Trichomonas vaginalis G3
Length = 184
Score = 42.7 bits (96), Expect = 0.007
Identities = 22/68 (32%), Positives = 35/68 (51%)
Frame = +3
Query: 306 DLSLVKMKSMFAIGFAFTALLSMFNSIFDGRVVAKLPFYPISWIQGLSHRNLPGDDYTDC 485
D+S M +G + M F +V KLPF S ++ L+HR+L +D +D
Sbjct: 85 DISQAVMLPKMVLGILPWLIPFMLKDYFKNKVCLKLPFEVTSSLKFLTHRDLETEDLSDA 144
Query: 486 SFIFLYIL 509
SF F+++L
Sbjct: 145 SFFFVFML 152
>UniRef50_A2D7Z5 Cluster: Putative uncharacterized protein; n=1;
Trichomonas vaginalis G3|Rep: Putative uncharacterized
protein - Trichomonas vaginalis G3
Length = 188
Score = 38.3 bits (85), Expect = 0.16
Identities = 16/47 (34%), Positives = 28/47 (59%)
Frame = +3
Query: 372 MFNSIFDGRVVAKLPFYPISWIQGLSHRNLPGDDYTDCSFIFLYILV 512
+ S F+ +V K PF I + ++H+ L G+DYT+ SF ++ L+
Sbjct: 107 IIGSFFENKVCIKFPFSVIYPLNKMTHKGLDGEDYTEGSFSSVFFLM 153
>UniRef50_A1ZHT1 Cluster: Major facilitator family transporter,
putative; n=3; Sphingobacteriales|Rep: Major facilitator
family transporter, putative - Microscilla marina ATCC
23134
Length = 419
Score = 33.5 bits (73), Expect = 4.5
Identities = 17/52 (32%), Positives = 27/52 (51%)
Frame = +3
Query: 312 SLVKMKSMFAIGFAFTALLSMFNSIFDGRVVAKLPFYPISWIQGLSHRNLPG 467
+++++ SM IG A T L+ M ++F G V + +W LSH N G
Sbjct: 308 AIIQLISMVIIGMATTPLMLMMGAVFFGIAVGIMSPTAFAWTIDLSHDNARG 359
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 549,404,746
Number of Sequences: 1657284
Number of extensions: 9549916
Number of successful extensions: 26307
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 25521
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 26303
length of database: 575,637,011
effective HSP length: 98
effective length of database: 413,223,179
effective search space used: 49173558301
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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