BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e96h0270 (748 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HS... 33 0.20 At2g29500.1 68415.m03583 17.6 kDa class I small heat shock prote... 33 0.20 At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HS... 33 0.27 At1g53540.1 68414.m06074 17.6 kDa class I small heat shock prote... 32 0.35 At4g21870.1 68417.m03163 26.5 kDa class P-related heat shock pro... 31 0.81 At4g14830.1 68417.m02280 expressed protein 29 3.3 At4g25434.1 68417.m03661 MutT/nudix family protein similar to SP... 29 4.3 At1g66720.1 68414.m07584 S-adenosyl-L-methionine:carboxyl methyl... 28 5.7 >At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HSP17.4-CI) identical to 17.4 kDa class I heat shock protein SP:P19036 from [Arabidopsis thaliana] Length = 156 Score = 33.1 bits (72), Expect = 0.20 Identities = 15/35 (42%), Positives = 25/35 (71%) Frame = +1 Query: 403 SRQFVRRYALPEGAAPETVESRLSSDGVLTITAPR 507 S +F+RR+ LPE A E V++ + +GVL++T P+ Sbjct: 108 SGKFMRRFRLPENAKVEEVKASM-ENGVLSVTVPK 141 >At2g29500.1 68415.m03583 17.6 kDa class I small heat shock protein (HSP17.6B-CI) contains Pfam PF00011: Hsp20/alpha crystallin family; identified in Scharf, K-D., et al, Cell Stress & Chaperones (2001) 6: 225-237. Length = 153 Score = 33.1 bits (72), Expect = 0.20 Identities = 15/35 (42%), Positives = 23/35 (65%) Frame = +1 Query: 403 SRQFVRRYALPEGAAPETVESRLSSDGVLTITAPR 507 S QF RR+ LPE + V++ + +GVLT+T P+ Sbjct: 105 SGQFTRRFRLPENVKMDQVKAAM-ENGVLTVTVPK 138 >At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HSP18.1-CI) identical to 18.2 kDa class I heat shock protein (HSP 18.2) (SP:P19037)[Arabidopsis thaliana]; contains Pfam profile: PF00011 Hsp20/alpha crystallin family Length = 161 Score = 32.7 bits (71), Expect = 0.27 Identities = 15/35 (42%), Positives = 24/35 (68%) Frame = +1 Query: 403 SRQFVRRYALPEGAAPETVESRLSSDGVLTITAPR 507 S +F+RR+ LPE A E V++ + +GVLT+ P+ Sbjct: 111 SGKFMRRFRLPENAKMEEVKATM-ENGVLTVVVPK 144 >At1g53540.1 68414.m06074 17.6 kDa class I small heat shock protein (HSP17.6C-CI) (AA 1-156) identical to (17.6 kDa class I heat shock protein (HSP 17.6) (AA 1-156)(SP:P13853) (GI:4376161) (Arabidopsis thaliana) (Nucleic Acids Res. 17 (19), 7995 (1989)) Length = 157 Score = 32.3 bits (70), Expect = 0.35 Identities = 14/35 (40%), Positives = 24/35 (68%) Frame = +1 Query: 403 SRQFVRRYALPEGAAPETVESRLSSDGVLTITAPR 507 S +F RR+ LPE A E +++ + +GVL++T P+ Sbjct: 109 SGKFTRRFRLPENAKMEEIKASM-ENGVLSVTVPK 142 >At4g21870.1 68417.m03163 26.5 kDa class P-related heat shock protein (HSP26.5-P) contains Pfam profile: PF00011 Hsp20/alpha crystallin family: identified in Scharf, K-D., et al,Cell Stress & Chaperones (2001) 6: 225-237. Length = 134 Score = 31.1 bits (67), Expect = 0.81 Identities = 20/81 (24%), Positives = 33/81 (40%) Frame = +1 Query: 277 DKFQVNLDVQHFSPEEISVKTADGYIVVXXXXXXXXXXXXYISRQFVRRYALPEGAAPET 456 D ++D+ EEI V+ D ++ + F R++ LPE Sbjct: 35 DSHTFSVDLPGLRKEEIKVEIEDSIYLIIRTEATPMSPPDQPLKTFKRKFRLPESIDMIG 94 Query: 457 VESRLSSDGVLTITAPRRYPT 519 + + DGVLT+ P+R T Sbjct: 95 ISAGYE-DGVLTVIVPKRIMT 114 >At4g14830.1 68417.m02280 expressed protein Length = 152 Score = 29.1 bits (62), Expect = 3.3 Identities = 13/31 (41%), Positives = 16/31 (51%) Frame = +1 Query: 415 VRRYALPEGAAPETVESRLSSDGVLTITAPR 507 V R+ LPE PE V DG L +T P+ Sbjct: 109 VWRFRLPESTRPELVTVDCDGDGELIVTVPK 139 >At4g25434.1 68417.m03661 MutT/nudix family protein similar to SP|P53370 Nucleoside diphosphate-linked moiety X motif 6 {Homo sapiens}; contains Pfam profile PF00293: NUDIX domain Length = 304 Score = 28.7 bits (61), Expect = 4.3 Identities = 11/28 (39%), Positives = 14/28 (50%) Frame = -1 Query: 652 EQIKMHHCKPPYLFSASWVPSLWSLISL 569 E + HH +P YL W+P S I L Sbjct: 80 EGFRYHHAEPTYLMLVYWIPEAESTIPL 107 >At1g66720.1 68414.m07584 S-adenosyl-L-methionine:carboxyl methyltransferase family protein similar to defense-related protein cjs1 [Brassica carinata][GI:14009292][Mol Plant Pathol (2001) 2(3):159-169] Length = 352 Score = 28.3 bits (60), Expect = 5.7 Identities = 11/26 (42%), Positives = 16/26 (61%) Frame = +2 Query: 413 SSDVTRCLKARRLRLWNRDCHQTGFS 490 +S + + + R LWNRD H TGF+ Sbjct: 151 TSKIPKGISDRNSPLWNRDMHCTGFN 176 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,281,670 Number of Sequences: 28952 Number of extensions: 342027 Number of successful extensions: 976 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 954 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 976 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1653386488 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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