Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0257
(721 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 233 2e-62
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 127 2e-30
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 124 2e-29
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 117 1e-27
SPACUNK4.12c |mug138||metallopeptidase|Schizosaccharomyces pombe... 34 0.018
SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 2.7
SPBC1861.09 |ppk22||serine/threonine protein kinase Ppk22 |Schiz... 27 2.7
SPAC4D7.03 |pop2|sud1|F-box/WD repeat protein Pop2|Schizosacchar... 27 3.6
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 26 4.7
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 26 4.7
SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyc... 26 6.2
SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 25 8.2
SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 25 8.2
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 233 bits (570), Expect = 2e-62
Identities = 102/140 (72%), Positives = 119/140 (85%)
Frame = +2
Query: 65 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGQVR 244
MREIVHIQAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GG+
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
Query: 245 APRLLVDLEPGTMDSVRSGPFGQIFRPXNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV 424
+LVDLEPGTMD+V+SG FG +FRP N ++GQSGAGN WAKGHYTEGAEL D+VLDVV
Sbjct: 61 PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120
Query: 425 RKEAESCDCLQGFQLTHSLG 484
R+EAE+CD LQGFQLTHSLG
Sbjct: 121 RREAEACDALQGFQLTHSLG 140
Score = 102 bits (245), Expect = 4e-23
Identities = 46/60 (76%), Positives = 53/60 (88%)
Frame = +3
Query: 504 GHLLISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDEXYCIDNXGL 683
G LL+SKIREEYPDR+M T+SV P+PK SDTVVEPYNATLS+HQLVEN+DE +CIDN L
Sbjct: 148 GTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEAL 207
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 127 bits (306), Expect = 2e-30
Identities = 60/142 (42%), Positives = 84/142 (59%), Gaps = 2/142 (1%)
Frame = +2
Query: 65 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLER--INVYYNEASGGQ 238
MREI+ I GQ G QIG WE+ EHGI P G + ++ Q + +++E G+
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 239 VRAPRLLVDLEPGTMDSVRSGPFGQIFRPXNFVFGQSGAGNNWAKGHYTEGAELVDSVLD 418
+ VDLEP +D VR+GP+ +F P + G+ A NN+A+GHYT G ELVD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 419 VVRKEAESCDCLQGFQLTHSLG 484
+R+ A++C LQGF + HS G
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFG 142
Score = 52.4 bits (120), Expect = 6e-08
Identities = 22/58 (37%), Positives = 37/58 (63%)
Frame = +3
Query: 501 YGHLLISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDEXYCIDN 674
+G LL+ ++ EY + +SV P+P+VS +VVEPYN+ L+ H ++ D + +DN
Sbjct: 149 FGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDN 206
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 124 bits (298), Expect = 2e-29
Identities = 58/146 (39%), Positives = 85/146 (58%), Gaps = 6/146 (4%)
Frame = +2
Query: 65 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 226
MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60
Query: 227 SGGQVRAPRLLVDLEPGTMDSVRSGPFGQIFRPXNFVFGQSGAGNNWAKGHYTEGAELVD 406
G+ + VDLEP +D VR+GP+ +F P V G+ A NN+A+GHYT G E++D
Sbjct: 61 GQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMID 120
Query: 407 SVLDVVRKEAESCDCLQGFQLTHSLG 484
SVL+ +R+ A++C LQGF + HS G
Sbjct: 121 SVLERIRRMADNCSGLQGFLVFHSFG 146
Score = 54.8 bits (126), Expect = 1e-08
Identities = 23/57 (40%), Positives = 38/57 (66%)
Frame = +3
Query: 504 GHLLISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDEXYCIDN 674
G LL+ ++ EY + +SV P+P+VS +VVEPYN+ L+ H ++N+D + +DN
Sbjct: 154 GALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDN 210
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 117 bits (282), Expect = 1e-27
Identities = 51/140 (36%), Positives = 89/140 (63%), Gaps = 2/140 (1%)
Frame = +2
Query: 68 REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGQVRA 247
REI+ +QAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62
Query: 248 PRLLVDLEPGTMDSVRSGPFGQIFRPXNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDV 421
+L+DLEP ++++ S +G ++ P N + ++ GAGNNWA G Y+ + + ++D+
Sbjct: 63 RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDM 121
Query: 422 VRKEAESCDCLQGFQLTHSL 481
+ +EA+ D L+GF L HS+
Sbjct: 122 IDREADGSDSLEGFSLLHSI 141
Score = 57.2 bits (132), Expect = 2e-09
Identities = 26/61 (42%), Positives = 38/61 (62%), Gaps = 1/61 (1%)
Frame = +3
Query: 504 GHLLISKIREEYPDRIMNTYSVVP-SPKVSDTVVEPYNATLSVHQLVENTDEXYCIDNXG 680
G L+ ++ + YP +I+ TYSV P S VSD VV+PYN+ L++ +L N D +DN
Sbjct: 150 GSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAA 209
Query: 681 L 683
L
Sbjct: 210 L 210
>SPACUNK4.12c |mug138||metallopeptidase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 969
Score = 34.3 bits (75), Expect = 0.018
Identities = 26/77 (33%), Positives = 42/77 (54%), Gaps = 11/77 (14%)
Frame = +3
Query: 474 TRSXWHRFRYGHLLISKIREEYP-DRIMNTYSVVP--SPKVSDTVVE---PYN--ATLSV 629
TR ++Y H++ SK++ EYP D+++ SV+ PK VVE P N A L+
Sbjct: 384 TRQKTPAYQYAHVVASKLQREYPRDKVLYYSSVLTEFDPKGIQEVVESLRPNNFFAILAA 443
Query: 630 HQL---VENTDEXYCID 671
H + ++N ++ Y ID
Sbjct: 444 HSIEKGLDNKEKFYGID 460
>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 428
Score = 27.1 bits (57), Expect = 2.7
Identities = 10/34 (29%), Positives = 22/34 (64%)
Frame = +2
Query: 104 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 205
N++G E+++++ +DPT A + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166
>SPBC1861.09 |ppk22||serine/threonine protein kinase Ppk22
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 526
Score = 27.1 bits (57), Expect = 2.7
Identities = 17/70 (24%), Positives = 32/70 (45%)
Frame = +2
Query: 179 DSDLQLERINVYYNEASGGQVRAPRLLVDLEPGTMDSVRSGPFGQIFRPXNFVFGQSGAG 358
DS L R++ + G + PR L++ M ++R + P N++FG++ G
Sbjct: 80 DSRPSLRRVSSAPDSHKGVEAPPPRPLIN-----MSNIRKAQVKILKNPGNYIFGRTEYG 134
Query: 359 NNWAKGHYTE 388
G+ T+
Sbjct: 135 KRTYSGNSTK 144
>SPAC4D7.03 |pop2|sud1|F-box/WD repeat protein
Pop2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 703
Score = 26.6 bits (56), Expect = 3.6
Identities = 21/63 (33%), Positives = 29/63 (46%), Gaps = 1/63 (1%)
Frame = +3
Query: 489 HRFRYGHLLISKIREEY-PDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDEXYC 665
H FR GH+ I + E P R++ + P V + TL V +L +NTD Y
Sbjct: 425 HIFR-GHISIIRCLEILVPSRLIRHGVEIVEPDQPYIVSGSRDHTLRVWKLPKNTDPPYL 483
Query: 666 IDN 674
DN
Sbjct: 484 PDN 486
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 26.2 bits (55), Expect = 4.7
Identities = 12/30 (40%), Positives = 19/30 (63%)
Frame = +2
Query: 368 AKGHYTEGAELVDSVLDVVRKEAESCDCLQ 457
A+GH G ELV + D +RK++E+ L+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 26.2 bits (55), Expect = 4.7
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = +2
Query: 299 GPFGQIFRPXNFVFGQSGAG-NNWAKGHYTEGAEL 400
GP+G +F P F+F +G NW+ Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190
>SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 587
Score = 25.8 bits (54), Expect = 6.2
Identities = 14/31 (45%), Positives = 15/31 (48%)
Frame = +3
Query: 192 SWSASMYTTMKPPAGKYVPRASSSTWSPAPW 284
S +AS PP SSST SPAPW
Sbjct: 396 SGAASPQAGHPPPWAAASTSVSSSTSSPAPW 426
>SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1063
Score = 25.4 bits (53), Expect = 8.2
Identities = 12/44 (27%), Positives = 20/44 (45%)
Frame = +2
Query: 320 RPXNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDC 451
RP +F G++ G + E D ++ + + ESCDC
Sbjct: 955 RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997
>SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 255
Score = 25.4 bits (53), Expect = 8.2
Identities = 13/32 (40%), Positives = 16/32 (50%)
Frame = -2
Query: 357 PAPDCPKTKLXGRKICPKGPERTESMVPGSKS 262
P+ PK L R I P GPE + + GS S
Sbjct: 20 PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,737,764
Number of Sequences: 5004
Number of extensions: 52866
Number of successful extensions: 169
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 153
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 164
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 337208592
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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